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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

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New Approaches for the analysis of confluent cell layers with quantitative phase digital holographic microscopy 13

New Approaches for the analysis of confluent cell layers wit...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Pohl, L. Kaiser, M. Ketelhut, S. Pereira, S. Goycoolea, F. Kemper, Bjoern Univ Munster Fac Med Biomed Technol Ctr Mendelstr 17 D-48149 Munster Germany Univ Munster Inst Plant Biol & Biotechnol Schlossgarten 3 D-48149 Munster Germany

ISBN: (纸本)9781628419474

Digital holographic microscopy (DHM) enables high resolution non-destructive inspection of technical surfaces and minimally-invasive label-free live cell imaging. However, the analysis of confluent cell layers represents a challenge as quantitative DHM phase images in this case do not provide sufficient information for image segmentation, determination of the cellular dry mass or calculation of the cell thickness. We present novel strategies for the analysis of confluent cell layers with quantitative DHM phase contrast utilizing a histogram based-evaluation procedure. The applicability of our approach is illustrated by quantification of drug induced cell morphology changes and it is shown that the method is capable to quantify reliable global morphology changes of confluent cell layers.

关键词： digital holographic microscopy quantitative phase imaging live cell imaging minimally-invasive label-free stain-free confluent cell layers

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A comparison of methods for optical sectioning using structured illumination microscopy

A comparison of methods for optical sectioning using structu...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Thomas, Benjamin Momany, Michelle Kner, Peter Univ Georgia Coll Engn Athens GA 30602 USA Univ Georgia Dept Plant Biol Athens GA 30602 USA

ISBN: (纸本)9780819493583

Structured Illumination microscopy is a simple and effective method to remove out-of-focus light in widefield fluorescence microscopy. Neil et al. originally proposed a simple square-law method for calculating the optically sectioned image from the three raw images with the structured illumination pattern super-imposed. However, the Neil method does not make the most efficient use of the three raw images. The three structured illumination images can also be used to separate three copies of the image covering shifted regions of frequency space in a similar manner to that developed by Gustafsson et al. These can then be combined using a generalized Wiener filter to create an image with a well-behaved optical transfer function in which the missing cone has been filled in, providing optical sectioning. Here, we compare the Neil and Gustafsson methods and show that the Gustafsson method provides an image with higher fidelity and a better Signal to Noise Ratio (SNR) at low photon counts. We apply the two methods to images of fluorescent beads and GFP labeled septins in Aspergillus nidulans.

关键词： fluorescence microscopy structured illumination microscopy three-dimensional imaging bioimaging

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Adaptive Optics for Wide-field microscopy

Adaptive Optics for Wide-field Microscopy

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Bourgenot, C. Saunter, C. D. Girkin, J. M. Love, G. D. Univ Durham Dept Phys Ctr Adv Instrumentat Durham DH1 3LE England

ISBN: (纸本)9780819484413

We report on recent developments in the use of adaptive optics (AO) in wide-field microscopy to remove both system and sample induced aberrations. We describe progress on using both a full AO system and image optimization techniques (wavefront sensorless AO). In the latter system the determination of the best mirror shape is found via two routes. In the first an optimization algorithm using a Simplex search pattern is used with an initial random set of mirror shapes. We then explore the use of specific Zernike terms as our starting basis set. In both cases the final optimization performance is not affected by the choice of optimization metric. We then describe an open loop AO system in which the equivalent of a laser guide star is used as the light source for the wavefront sensor.

关键词： Optical microscopy Aberration correction Adaptive Optics

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Wide-Field microscopy using Microcamera Arrays

Wide-Field Microscopy using Microcamera Arrays

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Marks, Daniel L. Youn, Seo Ho Son, Hui S. Kim, Jungsang Brady, David J. Duke Univ Dept Elect & Comp Engn Durham NC 27708 USA

ISBN: (纸本)9780819493583

A microcamera is a relay lens paired with image sensors. Microcameras are grouped into arrays to relay overlapping views of a single large surface to the sensors to form a continuous synthetic image. The imaged surface may be curved or irregular as each camera may independently be dynamically focused to a different depth. Microcamera arrays are akin to microprocessors in supercomputers in that both join individual processors by an optoelectronic routing fabric to increase capacity and performance. A microcamera may image ten or more megapixels and grouped into an array of several hundred, as has already been demonstrated by the DARPA AWARE Wide-Field program with multiscale gigapixel photography. We adapt gigapixel microcamera array architectures to wide-field microscopy of irregularly shaped surfaces to greatly increase area imaging over 1000 square millimeters at resolutions of 3 microns or better in a single snapshot. The system includes a novel relay design, a sensor electronics package, and a FPGA-based networking fabric. Biomedical applications of this include screening for skin lesions, wide-field and resolution-agile microsurgical imaging, and microscopic cytometry of millions of cells performed in situ.

关键词： Wide-field microscopy camera array image processing agile resolution

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Measurement of the Polarimetric Response of Suspended Gallium Doped Silicon Nanowires

Measurement of the Polarimetric Response of Suspended Galliu...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Theisen, Michael J. McIntyre, Brian L. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA Univ Rochester URnano Rochester NY USA

ISBN: (纸本)9780819488701

We describe an investigation of the polarimetric properties of suspended gallium doped silicon (Si:Ga) nanowires. Wire fabrication has been done with a combined gallium implantation (using a focused ion beam) and subsequent reactive ion and wet etches. A polarimetric microscope has been built and calibrated. Measurement of the polarimetric response shows a high reflectivity and strong retardance on reflection, with some samples showing low diattenuation, in contrast to conventional wire grid polarizers.

关键词： Polarimetry microscopy Nanowires

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Confocal Fluorescence lifetime imaging microscopy based on a realtime sampling method

Confocal Fluorescence lifetime imaging microscopy based on a...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Bae, Yoon. S. Lee, Dongsoo Moon, Sucbei Won, Young Jae Kim, Dug Y. Gwangju Inst Sci & Technol Dept Informat & Commun 1 Oryong Dong Kwangju 500712 South Korea

ISBN: (纸本)9780819465566

We present a fluorescence lifetime imaging microscope (FLIM) based on a real-time waveform acquisition method. The fluorophores were excited by a 635-mn gain-switched laser diode,, which produced short pulses with duration similar to 50 ps in a 20-MEz repetition rate. The fluorescence signals were detected by a silicon avalanche photo-diode (APD) in addition to a wide-band electric amplifier. The converted electric pulses were sampled by a high-speed digitizer of which sampling rate was 2 GS/s. In order to reduce the sampling interval for analyzing sub-nanosecond lifetimes, an interleaved data acquisition technique was used. The effective sampling rate was increased to 10 GS/s. In addition, the impulse response was measured simultaneously with the lifetime signals by an interleaving manner and was used in calibration of the system. By using these methods, accurate lifetime information was acquired in a short time less than 8 mu s.

关键词： confocal microscopy fluorescence lifetime imaging microscopy interleaved data acquisition

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Confocal scanning microscopy with multiple optical probes for high speed measurements and better imaging - art. no. 686115

Confocal scanning microscopy with multiple optical probes fo...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Chun, Wanhee Lee, SeungWoo Gweon, Dae-Gab Korea Adv Inst Sci & Technol Div Mech Engn Nano Opto Mechatron Lab Taejon 305701 South Korea

ISBN: (纸本)9780819470362

Confocal scanning microscopy (CSM) needs a scanning mechanism because only one point information of specimen can be obtained. Therefore the speed of the confocal scanning microscopy is limited by the speed of the scanning tool. To overcome this limitation from scanning tool we propose another scanning mechanism. We make three optical probes in the specimen under confocal condition of each point. Three optical probes are moved by beam scanning mechanism with shared resonant scanning mirror (RM) and galvanometer driven mirror (GM). As each optical probe scan allocated region of the specimen, information from three points is obtained simultaneously and image acquisition time is reduced. Therefore confocal scanning microscopy with multiple optical probes is expected to have three times faster speed of the image acquisition than conventional one. And as another use, multiple optical probes to which different light wavelength is applied can scan whole same region respectively. It helps to obtain better contrast image in case of specimens having different optical characteristics for specific light wavelength. In conclusion confocal scanning microscopy with multiple optical probes is useful technique for views of image acquisition speed and image quality.

关键词： confocal scanning microscopy multi point scanning multiple optical probes high speed measurement better image quality color imaging

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Reducing noise in extended depth of field microscope images by optical manipulation of the point spread function

Reducing noise in extended depth of field microscope images ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Zahreddine, Ramzi N. Cormack, Robert H. Cogswell, Carol J. Univ Colorado Dept Elect Comp & Energy Engn Boulder CO 80309 USA

ISBN: (纸本)9780819484413

This work describes improved methods and algorithms for implementing extended depth of field (EDF) microscopy through point spread function (PSF) engineering. It utilizes adaptive optics to create a test bed on which to evaluate new phase shapes for EDF. Being able to quickly and cheaply design novel PSFs is essential to overcome limitations of EDF that have prevented the technology from reaching mainstream use. Further improvement is made by reducing the noise normally seen in EDF images. Computational optics principles are used to first encode the noise with an identifiable pattern and a specially-tailored non-linear algorithm then removes the noise. This approach improves a microscope's imaging capabilities in photon-starved applications such as live-cell fluorescence and object tracking.

关键词： Extended Depth of Field PSF Engineering 3D microscopy Adaptive Optics Neural Networks Noise Removal

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Object segmentation, quantification, counting, and tracking

Object segmentation, quantification, counting, and tracking

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Abu-Tarif, A Khiznichenko, V Northan, B Holmes, T Yoon, P Girroir, J Brathwaite, P AutoQuant Imaging Inc Watervliet NY 12189 USA

ISBN: (纸本)0819452327

A complete system for object segmentation, counting, quantification, and tracking from microscopic images was implemented. We found that image deconvolution and reconstruction operations are essential to the success of any general-purpose segmentation algorithm and hence are of paramount importance for a counting and tracking software system. Wavelet-based image enhancement, background equalization, and noise suppression routines are the components in our novel general-purpose segmentation algorithm. Simple object recognition based on averages and preset tolerances suffices for most applications. As expected, boundary smoothing is important if watershed-based blob separation is to be used. One of the challenges of a general-purpose counting and tracking system is the need for a large number of object quantification components (features). In tracking we found that incorporating weighted features into an error function improves the accuracy over just the path coherence criterion and that evaluating correspondences over multiple time frames improves the accuracy over using only two consecutive time frames.

关键词： deconvolution image reconstruction segmentation object counting tracking

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Compressive sensing spectral domain optical coherence tomography with dispersion compensation

Compressive sensing spectral domain optical coherence tomogr...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Xu, Daguang Huang, Yong Kang, Jin U. Johns Hopkins Univ Dept Elect & Comp Engn Baltimore MD 21218 USA

ISBN: (纸本)9780819498625

In this paper, we describe a novel CS method that incorporates dispersion compensation into the CS reconstruction of spectral domain OCT (SD OCT) signal. We show that A-scans with dispersion compensation can be obtained by multiplying the dispersion correcting term to the undersampled linear-in-wavenumber spectral data before the CS reconstruction. We also implemented fast CS reconstruction by taking the advantage of fast Fourier transform (FFT). The matrix-vector multiplication commonly used in the CS reconstruction is implemented by a two-step procedure. Compared to the CS reconstruction with matrix multiplication, our method can obtain dispersion compensated A-scan at least 5 times faster. Experimental results show that the proposed method can achieve high quality image with dispersion compensation.

关键词： Compressive sensing optical coherence tomography reconstruction algorithm image processing dispersion compensation

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