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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 559 条记录，以下是181-190 订阅

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Studying the illumination puzzle towards an isotropic increase of optical resolution - art. no. 686112

Studying the illumination puzzle towards an isotropic increa...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Cella, Francesca Ronzitti, Emiliano Vicidomini, Giuseppe Mondal, Parta Pratim Diaspro, Alberto Univ Genoa LAMBS IFOM MicroScoBio Res Ctr Dept Phys DIFI I-16145 Genoa Italy

ISBN: (纸本)9780819470362

The aim of this work is to propose and analyze optical schemes to obtain an improvement of resolution in optical fluorescence microscopy. This goal can be achieved by implementing interfering illumination beams. We start from the insertion, on the illumination arm of the confocal microscope, of appropriately phase plates inducing laterally interfering beams, and then we propose to exploit two-photon excitation, too. We plan to implement solutions for shaping also the axial component of the point spread function by use of phase-only pupil filters and binary filters. In order to implement such schemes we use a computational simulation mainly based on a vectorial approach coupled to experimental procedures utilizing ultra-thin fluorescent layers and thick gels containing immobile fluorescent molecules as 2D and 3D phantoms, respectively. As well, image processing and successive views can be recombined to get a final isotropic improvement of resolution.

关键词： confocal microscopy two-photon excitation microscopy superresolution filters

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Evaluation of a combined two-color phase plate forming three-dimensional dark holes in super resolution microscopy 27

Evaluation of a combined two-color phase plate forming three...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Nagai, Koumei Maruyama, Takashi Kodaira, Akira Kumagai, Hiroshi Bokor, Nandor Iketaki, Yoshinori NTT Adv Technol Corp 3-1 Morinosato Wakamiya Atsugi Kanagawa 2430124 Japan Kitasato Univ Sch Allied Hlth Sci Phys 1-15-1 Kitasato Sagamihara Kanagawa 2520373 Japan Budapest Univ Technol & Econ Dept Phys Budafoki Ut 8 H-1111 Budapest Hungary Olympus Corp 2-3 Kuboyama Cho Hachioji Tokyo 1928512 Japan

ISBN: (纸本)9781510632547

In this study, a combined two-color phase plate (CTPP) was designed for super-resolution microscopy based on up conversion fluorescence depletion (FD), fabricated, and evaluated. It is composed of two types of phase plates, a spiral phase plate and an annular phase plate. A two-color phase plate modulates the phase of the erase beam while maintaining the phase of the pump beam. SRM performed using the proposed CTPP is expected to enable super resolution in both the focal plane and in the optical axis direction. Despite its complex structure, a highly accurate CTPP was obtained by using the exposure and etching processes used in semiconductor manufacturing.

关键词： Super-resolution microscopy Phase modulation Fluorescence depletion Optical-axis resolution

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Efficient and versatile aberration correction through sensorless adaptive optics 29

Efficient and versatile aberration correction through sensor...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXix

作者： Hu, Qi Hailstone, Martin Booth, Martin Univ Oxford Oxford England

ISBN: (纸本)9781510648043;9781510648036

Wavefront-sensorless adaptive optics methods are often used to correct phase aberrations in optical systems and thus to improve imaging quality. However, sensorless methods have an intrinsic disadvantage of requiring multiple images that can lead to non-desirable photo-bleaching. We have proposed a machine learning assisted aberration correction method which could correct aberrations consisting of not fewer than five Zernike modes with as few as two images. We showed that our method could be used in microscopes to provide instant aberration predictions when imaging biological samples of non-specific structures. We showed that compared to conventional function fitting sensorless adaptive optics methods, the new method corrected much faster with observable advantages. This novel method has a great potential to be used in any adaptive optics equipped microscopy for efficient sensorless aberration correction for biomedical microscopy.

关键词： Aberrations adaptive optics microscopy

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Development of micro-optical projection tomography for 3D analysis of single cells

Development of micro-optical projection tomography for 3D an...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Fauver, M Seibel, EJ Rahn, JR Patten, FW Nelson, AC Univ Washington Coll Engn Human Interface Technol Lab Seattle WA 98195 USA

ISBN: (纸本)0819452327

The Micro-Optical Projection Tomographic Microscope (muOPTM) is an instrument that is being developed for three-dimensional (3D) imaging of cells and subcellular components. The target application for the muOPTM is the early detection of lung cancer by revealing the complex 3D information about chromatin redistribution in the nucleus. The muOPTM employs a scanning objective lens (100x, N.A.=1.25) to create an extended depth-of-field image, similar to a shadowgram or projection, that we call a pseudo-projection. A large number of pseudoprojections (90+) are acquired, from which a tomographically reconstructed 3D image is computed using a filtered backprojection algorithm. The prototype muOPTM uses a single objective lens, so the object (cell) must be rotated for each new pseudo-projection. A custom microtube stage minimizes the lateral and axial motion of the sample tube during scanning and rotation so that registration between successive pseudo-projections is maintained. image processing methods are used to correct any remaining registration errors. The media inside and outside the tube are refractive index-matched to each other and to the tube (Deltan(avg) < 0.02). The index-matched materials are pressed between two flat parallel windows, providing a nearly distortion-free image. Custom phantoms using microspheres have been constructed and images of these 3D test targets acquired. The minimum resolvable feature size is better than 3 microns. The first 3D image of a cell using muOPTM is also shown.

关键词： optical tomography optical projection tomography three-dimensional imaging computed tomography microscopy depth of field cancer screening

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Extension of multidimensional microscopy to ultra-sensitive applications with maximum-likelihood analysis

Extension of multidimensional microscopy to ultra-sensitive ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Davis, Lloyd M. Shen, Guoqing Univ Tennessee Inst Space Ctr Laser Applicat Tullahoma TN 37388 USA

ISBN: (纸本)9780819465566

multidimensional fluorescence microscopy is finding service in forefront biological studies that require separation of images from different fluorophores. For example, commercial microscopes are available with multi-band analog detectors and user-friendly software for "linear unmixing" of species with overlapping emission spectra. To extend such techniques to ultrasensitive and single-molecule applications, we have developed a custom-built microscope, which incorporates two tunable-wavelength picosecond dye lasers for pulse-interleaved laser excitation, angle-tuned reflection of the laser beams from narrow-band Raman notch filters to introduce epi-illumination and provide strong rejection of scattered laser wavelengths, diffraction-limited confocal imaging with 3-dimensional piezo-scanning, an adjustable prism spectrometer for high-throughput resolution of collected fluorescence into 4 spectral bands, and a 4-channel high-quantum efficiency avalanche diode for sub-nanosecond-resolved single-photon detection. Custom software enables multi-band fluorescence correlation spectroscopy and identification of photon bursts for single-molecule detection. For umnixing of spectrally-overlapping signatures for ultrasensitive molecular imaging applications, we find that maximum-likelihood analysis can out-perform least-squares-based linear unmixing in the regime of low photon numbers per spectral/temporal channel. Also, the likelihood surface provides the confidence of the parameter estimates and the covariance of the species contributions. Monte Carlo simulations show that bias in the results of the analysis, which stems from the constraint that photon numbers should be positive, becomes more pronounced at low signal levels, for both maximum-likelihood and least-squares based uninixing.

关键词： fluorescence microscopy spectral-FLIM maximum-likelihood multidimensional microscopy photon counting

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A multiplexed confocal FLIM microscope with 4-taps time-gated imager 27

A multiplexed confocal FLIM microscope with 4-taps time-gate...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Richards, Morgan Shirakawa, Yuya Badr, Fares Kagawa, Keiichiro Kawahito, Shoji Fang, Qiyin McMaster Univ Dept Engn Phys 1280 Main St West Hamilton ON L8S 4L7 Canada Shizuoka Univ Res Inst Elect 3-5-1 Johoku Hamamatsu Shizuoka 4328011 Japan McMaster Univ Sch Biomed Engn 1280 Main St West Hamilton ON L8S 4L7 Canada

ISBN: (纸本)9781510632547

Combined with confocal imaging, Fluorescence lifetime imaging microscopy (FLIM) can achieve 3-dimensional optical sectional capability with sub-nanosecond lifetime information. As confocal FLIM acquires multi-dimensional data 4D (3D space + time), it is inherently slow. Recent developments in lock-in pixel imagers with time gated pixels show such detectors are capable of collecting as many as 8-time gates in a single pixel cycle. We present a multiplexed confocal FLIM microscope, equipped with a 4-taps time-gated lock-in pixel imager. The multiplexing setup allows the use of the sparse array with sub-nanosecond time-gating to achieve high throughput FLIM acquisition.

关键词： Multipoint Confocal Fluorescence FLIM Time-resolved CMOS image sensor lock-in pixel biomedical imaging

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Proceedings of Three-dimensional and multidimensional microscopy: image acquisition and processing V

Proceedings of Three-Dimensional and Multidimensional Micros...

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Proceedings of Three-dimensional and multidimensional microscopy: image acquisition and processing V

This Volume 3261 of the conference proceedings contains 36 papers. Topics discussed include optical instrumentation, microscopies for challenging biological imaging and measurement, improved methods and instruments fo... 详细信息

关键词： Imaging techniques

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Comparison of computational methods developed to address depth-variant imaging in fluorescence microscopy

Comparison of computational methods developed to address dep...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Rahman, Muhammad Mizanur Schaefer, Lutz H. Schuster, Dietwald Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Adv Imaging Mathodol Consultat Ontario ON Canada Univ Appl Sci Dept Math Regensburg Germany

ISBN: (纸本)9780819493583

In three-dimensional microscopy, the image formation process is inherently depth variant (DV) due to the refractive index mismatch between the imaging layers. In this study, we present a quantitative comparison among different image restoration techniques developed based on a depth-variant (DV) imaging model for fluorescence microscopy. The imaging models employed by these methods approximate DV imaging by either stratifying the object space (analogous to the discrete Fourier transform (DFT) "overlap-add" method) or image space (analogous to the DFT "overlap-save" method). We compare DV implementations based on maximum likelihood (ML) estimation and a previously developed expectation maximization algorithm to a ML conjugate gradient algorithm, using both these stratification approaches in order to assess their impact on the restoration methods. Simulations show that better restoration results are achieved with iterative methods implemented using the overlap-add method than with their implementation using the overlap-save method. However, the overlap-save method makes it possible to implement a non-iterative DV inverse filter that can trade off accuracy of the achieved result for computational speed. Results from a non-iterative regularized inverse filtering approach are also presented.

关键词： Depth-variant imaging 3D fluorescence microscopy spherical aberration image de-blurring

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On-line multi-spectral image analysis using unsupervised neural network

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Lu, T Tan, SZ Lu, RQ Lerner, JM In Harmony Technol Corp Petaluma CA 94952 USA

ISBN: (纸本)0819427004

The overwhelming size of a hyperspectral image creates serious problem for users to understand and utilize the data. A novel unsupervised neural network (UNN) model is presented. The UNN is designed to analyze the spectral contents of the multi-spectral images. The UNN automatically grows its layers and neurons by scanning the training images and by learning spectral features. The learning strategy is optimized to ensure fast convergence. At the end of learning, the UNN provides a table of the spectral content of the images. The image contents are categorized based on spectral similarities. The resulting spectral classes are then mapped onto the image, thus the UNN effectively compresses a hyperspectral image cube into a single image. The UNN is also able to automatically recognize objects by their spectral features. The ability of the UNN in identifying subtle spectral differences is shown.

关键词： hyperspectral multispectral image analysis unsupervised neural networks automated object recognition

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Novel contrast mechanism for label free super-resolution imaging 13

Novel contrast mechanism for label free super-resolution ima...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Alexandrov, Sergey A. McGrath, James Subhash, Hrebesh Boccafoschi, Francesca Giannini, Cinzia Leahy, Martin Natl Univ Ireland Sch Phys Tissue Opt & Microcirculat Imaging Grp Galway Ireland Univ Piemonte Orientale Dept Hlth Sci I-28100 Novara Italy CNR Inst Crystallog Via Amendola 122-O I-70126 Bari Italy

ISBN: (纸本)9781628419474

We present a novel super-resolution spectral encoding of spatial frequency (srSESF) approach to break the diffraction limit and dramatically improve resolution in lateral direction. The idea is to utilize additional information about the internal structure of the object to resolve features in the lateral direction. The novel contrast mechanism is realized by reconstruction and comparison of the axial spatial frequency (period) profiles at each image point to form superresolution image. As a result, small features, unresolved by conventional microscopy, can be visualized. Numerical simulation and experiments confirm the super-resolution abilities of the srSESF approach without applying any labels.

关键词： Optical microscopy label-free imaging super-resolution

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