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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 559 条记录，以下是11-20 订阅

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4D image processing in microscopy by combined methods

4D image processing in microscopy by combined methods

引用

conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Kriete, A Image Proc Lab Inst Anat & Cell Biol D-35385 Giessen Germany

ISBN: (纸本)081943535X

In time-lapse microscopy image quality is limited by instrumental and specimen characteristics. Moreover, an enormous amount of data may be accumulated. This challenges not only the development of new tools for image processing, but typically forces methods to be executed in combination. To remedy essential problems, an image processing pipeline is employed which features image restoration, image compression, visualization and analysis. To control the flow of information within this pipeline and to optimize the parameter settings like compression rates or number of iterations, the information content is evaluated on the basis of the ratio of signal and noise.

关键词： time-lapse microscopy 4D image processing compression image restoration visualization and analysis

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Optically sectioned images in widefield fluorescence microscopy

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Wilson, T Neil, MAA Juskaitis, R Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

An analysis is presented of the image formation in widefield fluorescence microscopy using standard light. The region of support of the resulting optical transfer function is discussed.

ISBN: (纸本)0819427004

An analysis is presented of the image formation in widefield fluorescence microscopy using standard light. The region of support of the resulting optical transfer function is discussed.

关键词： Luminescence microscopy Fringe analysis Spatial frequencies image acquisition Microscopes Imaging systems Optical transfer functions Point spread functions Roads

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Standards in digital microscopy

Standards in digital microscopy

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Kriete, A Shah, S Inst Anat & Cell Biol IPL D-35385 Giessen Germany

This paper discusses some aspects of standardization in digital microscopy. Included are image acquisition, use of image and graphics formats and data protocols in multidimensions, particular image analysis procedures... 详细信息

ISBN: (纸本)0819439398

关键词： Standards microscopy image acquisition image formats image processing

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image estimation for structured-illumination microscopy

Image estimation for structured-illumination microscopy

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Conchello, JA Oklahoma Med Res Fdn Program Mol Cell & Dev Biol Oklahoma City OK 73104 USA

ISBN: (纸本)0819456756

When recording three-dimensional (3D) images by the method of optical sectioning microscopy, each optical section contains the in-focus information plus out-of-focus contributions that obscure the in-focus detail and reduce contrast. There are several methods to remove or prevent the out-of-focus contributions from the stack of optical sections. One such method is image estimation -the use of a computer program based on a mathematical description of the microscope to remove the out-of-focus contributions. Another method is the use of structured illumination and a simple arithmetic operation to obtain a image that in which the out-of-focus contributions are greatly reduced. We derived a method for image estimation that uses the images collected from the structured-illumination microscope. The method improves the resolution of small detail over that possible with the structured illumination using the simple arithmetic formula.

关键词： image analysis Expectation maximization algorithms Microscopes microscopy 3D image processing Confocal microscopy image acquisition image processing Point spread functions Luminescence

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Fluorescence lifetime imaging microscopy based on a synchroscan streak camera

Fluorescence lifetime imaging microscopy based on a synchros...

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Qu, J Li, J Niu, HB Shenzhen Univ Inst Optoelect Shenzhen 518060 Guangdong Prov Peoples R China

ISBN: (纸本)0819452327

A new time-domain two-dimensional fluorescence lifetime detection method and a fluorescence lifetime imaging microscope that is based on a synchroscan streak camera are presented. This system can generate in parallel a complete two-dimensional fluorescence lifetime image without scanning the light. We demonstrate the acquisition of a fluorescence lifetime image of a semiconductor ET material sample at a temporal resolution of 2ps and spatial resolution of 4mum within 40ms.

关键词： fluorescence lifetime fluorescence lifetime imaging fluorescence lifetime imaging microscopy synchroscan scan streak camera temporal resolution spatial resolution

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High-resolution three-dimensional imaging using multiple nanometric probes

High-resolution three-dimensional imaging using multiple nan...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Dubois, A Moneron, G Lecaque, R Lequeux, F Boccara, AC Ecole Super Phys & Chim Ind Ville Paris CNRS UPR A0005 Lab Opt Phys F-75231 Paris 5 France

We present a new imaging modality using nanometric beads as multiple local probes. The positions of the beads are determined in three dimensions using white-light interference microscopy, by over-sampling and fitting ... 详细信息

ISBN: (纸本)0819447641

关键词： interference microscopy three-dimensional microscopy scanning probe microscopy

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Optical properties of spherical microlens

Optical properties of spherical microlens

引用

conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Volkov, UP Avetisyan, UA Baibyrin, VB Bespalova, NV Saratov State Tech Univ Saratov 410007 Russia

ISBN: (纸本)0819443603

A scanning optical far and near-field microscopes resolution is limited by focused light spot size used to scan a sample. As a focusing element in scanning microscopes are ordinary used lenses with dimensions of rather more than a light wavelength. The focus spot size of such lens is limited by well-known diffraction limit. However, if a lens dimension is comparable to a light wavelength the Fresnel's diffraction formula of focus spot size calculation is not suitable. At present, there are no data about theoretical or experimental studies of the small lenses and even about the ones fabrication methods. This paper is considers the fabrication method of small spherical glass lenses with diameters from I um to 100 um and discusses some theoretical approaches to the focus spot size calculation and it experimental measurement. The lenses fabrication method is based on melting of fine glass powder with oxygen-acetylene burner with subsequent lenses selection under conventional light microscope. To calculate a spot size of the small lens the Mie theory of light scattering in near field by small spherical particles has been used. The light intensity diagrams of sphere lens of various diameters calculated by developed program are presented.

关键词： spherical microlens scanning optical microscopy Mie theory optic

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Mapping organism expression levels at cellular resolution in developing Drosophila

Mapping organism expression levels at cellular resolution in...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Knowles, DW Keränen, S Biggin, MD Sudar, D Lawrence Berkeley Lab Dept Cell & Mol Biol Berkeley CA 94720 USA

ISBN: (纸本)0819443603

The development of an animal embryo is orchestrated by a network of genetically determined, temporal and spatial gene expression patterns that determine the animals final form. To understand such networks, we are developing novel quantitative optical imaging techniques to map gene expression levels at cellular and sub-cellular resolution within pregastrula Drosophila. Embryos at different stages of development are labeled for total DNA and specific gene products using different fluorophors and imaged in 3D with confocal microscopy. Innovative steps have been made which allow the DNA-image to be automatically segmented to produce a morphological mask of the individual nuclear boundaries. For each stage of development an "average" morphology is chosen to which images from different embryo are compared. The morphological mask is then used to quantify gene-product on a per nuclei basis. What results is an atlas of the relative amount of the specific gene product expressed within the nucleus of every cell in the embryo at the various stages of development. We are creating a quantitative database of transcription factor and target gene expression patterns in wild-type and factor mutant embryos with single cell resolution. Our goal is to uncover the rules determining how patterns of gene expression are generated.

关键词： confocal imaging image segmentation image registration quantitative fluorescence analysis regulatory gene networks Drosophila

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High resolution spectral self-interference fluorescence microscopy

High resolution spectral self-interference fluorescence micr...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Swan, AK Moiseev, L Tong, YJ Lipoff, SH Karl, WC Goldberg, BB Ünlü, MS Boston Univ Dept Elect & Comp Engn Boston MA 02215 USA

ISBN: (纸本)0819443603

We present a new method of fluorescence imaging, which yields nm-scale axial height determination and similar to15 nm axial resolution. The method uses the unique spectral signature of the fluorescent emission intensity well above a reflecting surface to determine vertical position unambiguously. We have demonstrated axial height determination with nm sensitivity by resolving the height difference of fluorescein directly on the surface or ontop of streptavidin. While different positions of fluorophores of different color are determined independently with nm precision, resolving the position of two fluorophores of the same color is a more convoluted problem due to the finite spectral emission widow of the fluorophores. Hence, for physically close (

关键词： fluorescence microscopy self interference spectroscopy

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Three-dimensional imagery using multiple nanometric probes

Three-dimensional imagery using multiple nanometric probes

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Moneron, G Dubois, A Boccara, AC Ecole Super Phys & Chim Ind Ville Paris Lab Opt Phys CNRS UPR A0005 F-75231 Paris 5 France

ISBN: (纸本)0819452327

We present a high-resolution imaging technique using nanometric beads as multiple scattering local probes. The positions of the beads are determined in three dimensions by white-light interference microscopy. The technique has been applied to study the deformation of gels under mechanical constraint. The location of Brownian moving beads has also been demonstrated with nanometer spatial precision and 10 mus acquisition time. High-resolution 3-D imaging of hollow structures explored by the beads in relatively transparent materials should be possible.

关键词： interference microscopy three-dimensional microscopy scanning probe microscopy

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