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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

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Dynamic phase imaging and processing of moving biological organisms

Dynamic phase imaging and processing of moving biological or...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Creath, Katherine Goldstein, Goldie 4D Technol Corp Tucson AZ 85706 USA

ISBN: (纸本)9780819488701

This paper describes recent advances in developing a new, novel interference Linnik microscope system and presents images and data of live biological samples. The specially designed optical system enables instantaneous 4-dimensional video measurements of dynamic motions within and among live cells without the need for contrast agents. "Label-free" measurements of biological objects in reflection using harmless light levels are possible without the need for scanning and vibration isolation. This instrument utilizes a pixelated phase mask enabling simultaneous measurement of multiple interference patterns taking advantage of the polarization properties of light enabling phase image movies in real time at video rates to track dynamic motions and volumetric changes. Optical thickness data are derived from phase images after processing to remove the background surface shape to quantify changes in cell position and volume. Data from a number of different pond organisms will be presented, as will measurements of human breast cancer cells with the addition of various agents that break down the cells. These data highlight examples of the image processing involved and the monitoring of different biological processes.

关键词： phase imaging interference microscopy polarization interferometry cellular imaging cell dynamics optical thickness measurement label-free imaging 4D microscopy

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Star Test Polarimetry using Stress-Engineered Optical Elements

Star Test Polarimetry using Stress-Engineered Optical Elemen...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Ramkhalawon, Roshita Beckley, Amber M. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA

ISBN: (纸本)9780819488701

Stress-engineered optical elements have potential applications in snapshot polarimetry, in which a single irradiance image is used to measure a spatially varying polarization. In this paper, we present star test polarimetry which is a method of polarization retrieval by analyzing a single frame point spread function. A trigonally stressed window placed at the pupil plane of an imaging system is used to produce point spread functions which are then processed to extract the polarization state of the incoming beam under investigation. We outline several methods which are used to recover the Stokes parameters of a beam of unknown polarization from the irradiance distribution of its point spread function.

关键词： Polarimetry microscopy Unconventional Polarization states

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Performance evaluation of point-spread function engineering to reduce the impact of depth-induced aberrations on extended depth-of-field microscopy

Performance evaluation of point-spread function engineering ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Yuan, Shuai Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA

ISBN: (纸本)9780819488701

In this study, we evaluated a point-spread function (PSF) engineered using wave front encoding (WFE) and a generalized cubic phase mask (GCPM) design selected to reduce the impact of depth-induced spherical aberration (SA) on extended depth-of-field (EDOF) microscopy with high NA lenses. Mean-square-error based metrics computed from three-dimensional (3D) depth-variant WFE-PSFs with increasing amounts of SA were used to quantify the engineered PSF's sensitivity to SA and to compare it to the sensitivity of the cubic-phase mask (CPM) PSF traditionally used for EDOF microscopy. The potential performance of the engineered PSF with resilience to SA was further evaluated with simulations in which EDOF images of a 3D object were obtained by processing WFE images with and without SA. A qualitative and quantitative comparison of the EDOF images with the true object show that the WFE-PSF engineering with the selected GCPM design provides better performance in reducing the impact of SA. In addition, the GCPM-based EDOF images do not suffer from the known lateral shift of object features located away from the plane of focus encountered in traditional CPM-based EDOF images.

关键词： Extended depth-of-field microscopy point-spread function engineering wavefront encoding spherical aberration

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Measurement of the Polarimetric Response of Suspended Gallium Doped Silicon Nanowires

Measurement of the Polarimetric Response of Suspended Galliu...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Theisen, Michael J. McIntyre, Brian L. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA Univ Rochester URnano Rochester NY USA

ISBN: (纸本)9780819488701

We describe an investigation of the polarimetric properties of suspended gallium doped silicon (Si:Ga) nanowires. Wire fabrication has been done with a combined gallium implantation (using a focused ion beam) and subsequent reactive ion and wet etches. A polarimetric microscope has been built and calibrated. Measurement of the polarimetric response shows a high reflectivity and strong retardance on reflection, with some samples showing low diattenuation, in contrast to conventional wire grid polarizers.

关键词： Polarimetry microscopy Nanowires

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Restoration of high-resolution AFM images captured with broken probes

Restoration of high-resolution AFM images captured with brok...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Wang, Y. F. Corrigan, D. Forman, C. Jarvis, S. Kokaram, A. Univ Dublin Trinity Coll Dept Elect Engn Dublin 2 Ireland Univ Coll Dublin Conway Grp Nanoscale Funct Grp Dublin Ireland

ISBN: (纸本)9780819488701

A type of artefact is induced by damage of the scanning probe when the Atomic Force Microscope (AFM) captures a material surface structure with nanoscale resolution. This artefact has a dramatic form of distortion rather than the traditional blurring artefacts. Practically, it is not easy to prevent the damage of the scanning probe. However, by using natural image deblurring techniques in image processing domain, a comparatively reliable estimation of the real sample surface structure can be generated. This paper introduces a novel Hough Transform technique as well as a Bayesian deblurring algorithm to remove this type of artefact. The deblurring result is successful at removing blur artefacts in the AFM artefact images. And the details of the fibril surface topography are well preserved.

关键词： Atomic force microscopy Distortion Hough transforms Deconvolution image analysis image processing Convolution Gaussian filters image resolution Atomic force microscope

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Cell morphology classification in phase contrast microscopy image reducing halo artifact

Cell morphology classification in phase contrast microscopy ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Kang, Mi-Sun Song, Soo-Min Lee, Hana Kim, Myoung-Hee Ewha Womans Univ Dept Comp Sci & Engn Seoul 120750 South Korea

ISBN: (纸本)9780819488701

Since the morphology of tumor cells is a good indicator of their invasiveness, we used time-lapse phase-contrast microscopy to examine the morphology of tumor cells. This technique enables long-term observation of the activity of live cells without photobleaching and phototoxicity which is common in other fluorescence-labeled microscopy. However, it does have certain drawbacks in terms of imaging. Therefore, we first corrected for non-uniform illumination artifacts and then we use intensity distribution information to detect cell boundary. In phase contrast microscopy image, cell is normally appeared as dark region surrounded by bright halo ring. Due to halo artifact is minimal around the cell body and has non-symmetric diffusion pattern, we calculate cross sectional plane which intersects center of each cell and orthogonal to first principal axis. Then, we extract dark cell region by analyzing intensity profile curve considering local bright peak as halo area. Finally, we examined cell morphology to classify tumor cells as malignant and benign.

关键词： cell morphology phase contrast microscopy image halo artifact cell classification

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Time-Resolved Single Molecule microscopy coupled with Atomic Force microscopy

Time-Resolved Single Molecule Microscopy coupled with Atomic...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Koenig, M. Koberling, F. Schulz, O. Ros, R. Fore, S. Sackrow, M. Trautmann, S. Erdmann, R. PicoQuant GmbH Rudower Chaussee 29 D-12489 Berlin Germany Arizona State Univ Dept Phys Tempe AZ 85287 USA PicoQuant Photon North Amer Inc Westfield MA 01085 USA

ISBN: (纸本)9780819488701

Time-resolved confocal microscopy is well established to image spectral and spatial properties of samples in biology and material science. Atomic Force microscopy (AFM) in addition enables to investigate properties which are not optically addressable or are hidden by the diffraction limited optical resolution. We present a straight forward combination of single molecule sensitive time-resolved confocal microscopy with different commercially available AFMs. Besides an extra of information about for example a cell surface, the AFM tip can also be used to manipulate the sample on a nanometer scale down to the single molecule level.

关键词： Confocal microscopy Atomic Force microscopy AFM quenching time-resolved fluorescence sub diffraction single molecule

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Background and speckle suppression with a divided pupil and Nomarski prism for reflectance line-scanning confocal microscopy of human tissues

Background and speckle suppression with a divided pupil and ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Glazowski, Christopher Zavislan, James Rajadhyaksha, Milind Mem Sloan Kettering Canc Ctr Dermatol Serv New York NY 10022 USA Univ Rochester Inst Opt Rochester NY 14623 USA

ISBN: (纸本)9780819488701

We present a design of a line-scanning confocal microscope for reduced speckle and improved sectioning performance by the use of two pupil-modification techniques. The first is a divided-pupil configuration in which the illumination and detection paths are separate in object space except in the focal (optical sectioning) plane. The second technique is a novel implementation of a Nomarski prism and quarter-wave retarder, termed NRDIC, which has shown good results in point-scanning confocal microscopy and we will present its translation to line-scanning confocal microscopy. A stable turbid phantom that simulates the background-driven speckle was used for quantitative characterization. Compared to standard full pupil line-scanning, we show improvements in signal to background of 1.8 and 9 for NRDIC and divided pupil, respectively. Preliminary imaging in human skin in-vivo demonstrates the improvements in contrast and reduction of speckle for both the NRDIC and divided pupil modes.

关键词： microscopy turbid speckle confocal line-scanning divided pupil NRDIC

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Multimodal light-sheet microscopy for fluorescence live imaging

Multimodal light-sheet microscopy for fluorescence live imag...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Oshima, Y. Kajiura-Kobayashi, H. Nonaka, S. Ehime Univ Hosp Shitukawa Toon City Ehime 7910295 Japan Natl Inst Basic Biol Okazaki Aichi 4448585 Japan

ISBN: (纸本)9780819488701

Light-sheet microscopy, it is known as single plane illumination microscope (SPIM), is a fluorescence imaging technique which can avoid phototoxic effects to living cells and gives high contrast and high spatial resolution by optical sectioning with light-sheet illumination in developmental biology. We have been developed a multifunctional light-sheet fluorescence microscopy system with a near infrared femto-second fiber laser, a high sensitive image sensor and a high throughput spectrometer. We performed that multiphoton fluorescence images of a transgenic fish and a mouse embryo were observed on the light-sheet microscope. As the results, two photon images with high contrast and high spatial resolution were successfully obtained in the microscopy system. The system has multimodality, not only mutiphoton fluorescence imaging, but also hyperspectral imaging, which can be applicable to fluorescence unmixing analysis and Raman imaging. It enables to obtain high specific and high throughput molecular imaging in vivo and in vitro.

关键词： Light-sheet microscopy fluorescence microscopy SPIM DSLM Multiphoton microscopy hyperspectral imaging Raman imaging in vivo imaging

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Analysis of the Effects of Different Resampling Techniques for Optical Coherence Tomography

Analysis of the Effects of Different Resampling Techniques f...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Copeland, Christopher N. Ellerbee, Audrey K. Stanford Univ EL Ginzton Lab Stanford CA 94305 USA Stanford Univ Dept Elect Engn Stanford CA 94305 USA

ISBN: (纸本)9780819488701

Fourier domain optical coherence tomography (FD-OCT) uses interferometry and spatially coherent, polychromatic light to acquire cross-sectional images of scattering media such as biological tissue. Phase-sensitive derivatives of FD-OCT, such as spectral domain phase microscopy (SDPM), can perform quantitative phase imaging of cellular dynamics with sub-Angstrom sensitivity. FD-OCT and SDPM images are generated by taking the Fourier Transform of the raw spectral interferogram;the accuracy of the reconstruction depends on the choice of processing algorithms used, system noise, calibration and quantization errors. To decrease the processing time, the Fast Fourier transform (FFT) algorithm can be applied when the data are evenly sampled in wavenumber. Unfortunately, most OCT systems are designed for constant wavelength sampling, not constant wavenumber sampling. While there is general agreement on the qualitative superiority of some resampling methods over others, to the best of our knowledge there has been no study that compares these methods for OCT phase data. We examine the effects of various resampling techniques on simulated phase and amplitude OCT data. Given the trend towards high-speed imaging with OCT, the choice of resampling methods is critical, as one need balance processing time and image accuracy - not merely quality - when analyzing medical image data.

关键词： optical coherence tomography low coherence interferometry phase retrieval microscopy

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