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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 559 条记录，以下是261-270 订阅

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Pupil Engineering for a Confocal Reflectance Line-Scanning Microscope

Pupil Engineering for a Confocal Reflectance Line-Scanning M...

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Patel, Yogesh G. Rajadhyaksha, Milind DiMarzio, Charles A. Northeastern Univ Dept Elect & Comp Engn 440 Dana Bldg360 Huntington Ave Boston MA 02115 USA Northeastern Univ Dept Mech & Ind Engn Boston MA 02115 USA Serv Dermatol Dept Med Memorial Sloan Kettering Canc Ctr New York NY 10022 USA

ISBN: (纸本)9780819484413

Confocal reflectance microscopy may enable screening and diagnosis of skin cancers noninvasively and in real-time, as an adjunct to biopsy and pathology. Current confocal point-scanning systems are large, complex, and expensive. A confocal line-scanning microscope, utilizing a of linear array detector can be simpler, smaller, less expensive, and may accelerate the translation of confocal microscopy in clinical and surgical dermatology. A line scanner may be implemented with a divided-pupil, half used for transmission and half for detection, or with a full-pupil using a beamsplitter. The premise is that a confocal line-scanner with either a divided-pupil or a full-pupil will provide high resolution and optical sectioning that would be competitive to that of the standard confocal point-scanner. We have developed a confocal line-scanner that combines both divided-pupil and full-pupil configurations. This combined-pupil prototype is being evaluated to determine the advantages and limitations of each configuration for imaging skin, and comparison of performance to that of commercially available standard confocal point-scanning microscopes. With the combined configuration, experimental evaluation of line spread functions (LSFs), contrast, signal-to-noise ratio, and imaging performance is in progress under identical optical and skin conditions. Experimental comparisons between divided-pupil and full-pupil LSFs will be used to determine imaging performance. Both results will be compared to theoretical calculations using our previously reported Fourier analysis model and to the confocal point spread function (PSF). These results may lead to a simpler class of confocal reflectance scanning microscopes for clinical and surgical dermatology.

关键词： confocal microscopy Fourier optics point-scanning line-scanning full-pupil divided-pupil

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Semi-automated Algorithm for Localization of Dermal/Epidermal Junction in Reflectance Confocal microscopy images of Human Skin

Semi-automated Algorithm for Localization of Dermal/Epiderma...

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Kurugol, Sila Dy, Jennifer G. Rajadhyaksha, Milind Gossage, Kirk W. Weissman, Jesse Brooks, Dana H. Northeastern Univ 360 Huntington Av Boston MA 02115 USA Mem Sloan Kettering Canc Ctr Serv Dermatol New York NY 10065 USA Unilever Res Labs Trumbull CT 06611 USA

ISBN: (纸本)9780819484413

The examination of the dermis/epidermis junction (DEJ) is clinically important for skin cancer diagnosis. Reflectance confocal microscopy (RCM) is an emerging tool for detection of skin cancers in vivo. However, visual localization of the DEJ in RCM images, with high accuracy and repeatability, is challenging, especially in fair skin, due to low contrast, heterogeneous structure and high inter-and intra-subject variability. We recently proposed a semi-automated algorithm to localize the DEJ in z-stacks of RCM images of fair skin, based on feature segmentation and classification. Here we extend the algorithm to dark skin. The extended algorithm first decides the skin type and then applies the appropriate DEJ localization method. In dark skin, strong backscatter from the pigment melanin causes the basal cells above the DEJ to appear with high contrast. To locate those high contrast regions, the algorithm operates on small tiles (regions) and finds the peaks of the smoothed average intensity depth profile of each tile. However, for some tiles, due to heterogeneity, multiple peaks in the depth profile exist and the strongest peak might not be the basal layer peak. To select the correct peak, basal cells are represented with a vector of texture features. The peak with most similar features to this feature vector is selected. The results show that the algorithm detected the skin types correctly for all 17 stacks tested (8 fair, 9 dark). The DEJ detection algorithm achieved an average distance from the ground truth DEJ surface of around 4.7 mu m for dark skin and around 7-14 mu m for fair skin.

关键词： confocal reflectance microscopy image analysis skin classification

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Optical design of a snapshot high-sampling image Mapping Spectrometer (IMS) for hyperspectral microscopy

Optical design of a snapshot high-sampling Image Mapping Spe...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Gao, Liang S. Kester, Robert T. Tkaczyk, Tomasz S. Rice Univ Dept Bioengn Houston TX 77005 USA

ISBN: (纸本)9780819479662

A snapshot high-sampling image Mapping Spectrometer (IMS) is developed for hyperspectral microscopy, measuring datacube of dimensions 285x285x60 (x,y,lambda). microscopy IMS is designed to work at the Nyquist sampling limit to realize high resolution imaging. The spatial resolution is similar to 0.45 mu m with FOV similar to 130 mu m. The spectral working range is 500-700nm with similar to 8.3nm average spectral resolution. Preliminary tests have been implemented on its imaging performances.

关键词： Hyperspectral microscopy image Mapping Spectrometer

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Pupil Polarimetry using Stress-Engineered Optical Elements

Pupil Polarimetry using Stress-Engineered Optical Elements

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Beckley, Amber M. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA

ISBN: (纸本)9780819479662

Stress-engineered optical elements show fascinating and potentially useful effects when placed at the pupil plane of an imaging system. When illuminated by a beam of spatially uniform polarization, a snapshot (single measurement) polarimetry method can be constructed. We expand upon this method to perform snapshot pupil polarimetry for spatially varying pupil polarization. We present the theory for snapshot non-uniform pupil polarization measurement using a stress-engineered optical element, as well as simulation results.

关键词： Polarimetry microscopy Cylindrical Vector Beams

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Extended depth-of-field microscopy

Extended depth-of-field microscopy

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Smith, Christopher Botcherby, Edward Booth, Martin Juskaitis, Rimas Wilson, Tony Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

ISBN: (纸本)9780819479662

In this paper, we present details of a scanning two-photon fluorescence microscope we have built with a near-isotropic scan rate. This means that the focal spot can be scanned at high speed along any direction in the specimen, without introducing systematic aberrations. We present experimental point spread function measurements for this system using an Olympus 1.4 NA 60X oil immersion lens that demonstrates an axial range of operation greater than 70 mu m. We give details of a novel actuator device used to displace the focusing element and demonstrate axial scan responses up to 3.5 kHz. Finally, we present an application of this system in liquid crystal research to image the dynamic response of a nematic device during switching. Information about the director field at different levels in the device can be inferred from images acquired with a temporal resolution of 2.5 ms.

关键词： optical refocusing two-photon microscopy

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Gabor wavelet transform for dynamic analysis in digital holographic microscopy

Gabor wavelet transform for dynamic analysis in digital holo...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Zhong, Jingang Weng, Jiawen Hu, Cuiying Jinan Univ Inst Optoelect Engn Guangzhou 510632 Guangdong Peoples R China

ISBN: (纸本)9780819479662

Real time dynamic analysis of micro-object is one of the significant advantages of digital holographic microscopy, but the process of the spatial filtering limits the application of dynamic and automatic analysis in digital holographic microscopy. In this paper, a numerical reconstruction technique by means of Gabor wavelet transform (GWT) is presented for real time dynamic analysis. Appling the GWT to the hologram, the quantitative phase information of the micro-object is obtained automatically without the process of the spatial filtering. An experiment with a sequence of holograms of an animalcule is proposed for the dynamic and automatic analysis by employing the GWT method.

关键词： Digital holographic microscopy dynamic analysis wavelet transform full-field quantitative phase imaging

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Dual-modal optical projection tomography microscopy for cancer diagnosis

Dual-modal optical projection tomography microscopy for canc...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Miao, Qin Yu, Julia Meyer, Michael G. Rahn, J. Richard Neumann, Thomas Nelson, Alan C. Seibel, Eric J. Univ Washington Dept Bioengn Seattle WA 98195 USA VisGate Inc Gig Harbor WA 98335 USA Univ Washington Dept Engn Mech Seattle WA 98195 USA

ISBN: (纸本)9780819479662

A dual-modal optical projection tomography microscope (OPTM) is presented, which produces three-dimensional images of single cells with isometric high resolution both in fluorescence and absorption mode. Depth of field of a high numerical aperture objective is extended by scanning the focal plane through the sample in order to enable reconstruction by back-projection method. Cells are fixed, stained, and mixed with optical gel and injected into the capillary for imaging. Combining absorption and fluorescence mode allows us to image different aspects of the disease process. images of cells stained with both hematoxylin and fluorescence probes are shown. Registrations between two modes are discussed.

关键词： Three-dimensional microscopy Tomographic imaging Fluorescence biomarker imaging Coregistration

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Photobleaching Property of Confocal Laser Scanning microscopy with Masked Illumination

Photobleaching Property of Confocal Laser Scanning Microscop...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Kim, DongUk Moon, Sucbei Song, Hoseong Yang, Wenzhong Kim, Dug Y. Gwangju Inst Sci & Technol Dept Informat & Commun Kwangju 500712 South Korea

ISBN: (纸本)9780819479662

Confocal laser scanning microscopy (CLSM) has become the tool of choice for high-contrast fluorescence imaging in the study of the three-dimensional and dynamic properties of biological system. However, the high cost and complexity of commercial CLSMs urges many researchers to individually develop low cost and flexible confocal microscopy systems. The high speed scanner is an influential factor in terms of cost and system complexity. Resonant galvo scanners at several kHz have been commonly used in custom-built CLSMs. However, during the repeated illumination for live cell imaging or 3D image formation, photobleaching and image distortion occurred at the edges of the scan field may be more serious than the center due to an inherent property (e. g. sinusoidal angular velocity) of the scan mirror. Usually, no data is acquired at the edges due to large image distortion but the excitation beam is still illuminated. Here, we present the photobleaching property of CLSM with masked illumination, a simple and low cost method, to exclude the unintended excitation illumination at the edges. The mask with a square hole in its center is disposed at the image plane between the scan lens and the tube lens in order to decrease photobleaching and image distortion at the edges. The excluded illumination section is used as the black level of the detected signals for a signal quantizing step. Finally, we demonstrated the reduced photobleaching at the edges on a single layer of fluorescent beads and real-time image acquisition without a standard composite video signal by using a frame grabber.

关键词： Photobleaching confocal laser scanning microscopy video-rate 3D image masked illumination

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Improvement based on FDK reconstruction algorithm of conebeam CT

Improvement based on FDK reconstruction algorithm of conebea...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Miao, Hui Wang, Tingting Zhao, Huijuan Gao, Feng Tianjin Univ Coll Precis Instruments & Optoelect Engn Tianjin 300072 Peoples R China

ISBN: (纸本)9780819479662

As a typical 3D image algorithm, Feldkamp-David-Kress reconstruction algorithm is a filtered backprojection very similar to the 2D algorithm. Recent years, it has been widely used for its easy implementation and acceptable reconstruction precision for small cone-beam angle. For big cone-beam angles, however, the reconstruction exactness of horizontal planes will dramatically decrease with the distance from the central plane increases. Therefore, for improving the reconstruction precision in vertical direction, a similar reciprocal Gaussian function as the weighted factor was introduced into FDK algorithm in the article. The validity of the improved FDK algorithm was verified and evaluated through both the computer numerical simulation and the phantom model experiment. The quantitative analysis for the reconstruction results demonstrated that the reconstruction image using the improved FDK algorithm could primely revise the original reconstruction images and restore well closely to the tomography image of the prototype object.

关键词： FDK reconstruction cone-beam CT image reconstruction reconstruction algorithm

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Closed loop adaptive optics for microscopy without a wavefront sensor

Closed loop adaptive optics for microscopy without a wavefro...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Kner, Peter Winoto, Lukman Agard, David A. Sedat, John W. Univ Georgia Fac Engn Athens GA 30602 USA Univ Calif San Francisco Dept Biochem & Biophys Keck Adv Microscopy Lab San Francisco CA 94158 USA Univ Calif San Francisco Howard Hughes Med Inst San Francisco CA 94143 USA

ISBN: (纸本)9780819479662

A three-dimensional wide-field image of a small fluorescent bead contains more than enough information to accurately calculate the wavefront in the microscope objective back pupil plane using the phase retrieval technique. The phase-retrieved wavefront can then be used to set a deformable mirror to correct the point-spread function (PSF) of the microscope without the use of a wavefront sensor. This technique will be useful for aligning the deformable mirror in a widefield microscope with adaptive optics and could potentially be used to correct aberrations in samples where small fluorescent beads or other point sources are used as reference beacons. Another advantage is the high resolution of the retrieved wavefont as compared with current Shack-Hartmann wavefront sensors. Here we demonstrate effective correction of the PSF in 3 iterations. Starting from a severely aberrated system, we achieve a Strehl ratio of 0.78 and a greater than 10-fold increase in maximum intensity.

关键词： Adaptive Optics microscopy Biomedical Imaging

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