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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 559 条记录，以下是21-30 订阅

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image enhancement for a low cost TEM acquisition system.

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Randall, G Fernandez, A Trujillo-Cenoz, O Apelbaum, G Bertalmio, M Vazquez, L Malmierca, F Morelli, P Univ Republ Fac Ingn Inst Ingn Elect Montevideo Uruguay

This paper describes a method for the improvement of biological images acquired using a Transmission Electronic Microscope (TEM). Several techniques are presented that deal with noise reduction, artifact removal and n... 详细信息

ISBN: (纸本)0819427004

关键词： Transmission Electronic microscopy image processing non-uniform illumination

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Three-dimensional multiple nanometric local probes microscopy

Three-dimensional multiple nanometric local probes microscop...

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Moneron, G Dubois, A Boccara, AC CNRS UPA A0005 Ecole Super Phys Chim Ind Lab Opt Phys F-75231 Paris France

ISBN: (纸本)0819456756

We present a microscope set-up designed to produce three-dimensional images of the internal structures of various samples with high spatial resolution (a few tens of nanometers in axial and transverse directions). This level of resolution is reached by the use of nanometric sub-wavelength spherical gold beads as multiple local probes, dispersed in the hollow structures. The exploration, by Brownian motion, of the internal structures allows their three-dimensional reconstruction.

关键词： interference microscopy three-dimensional microscopy optical coherence tomography white-light interferometry scanning probe microscopy

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Scanning microscopy with extended depth of focus

Scanning microscopy with extended depth of focus

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Juskaitis, R Botcherby, EJ Wilson, T Univ Oxford Dept Engn Sci Oxford England

We demonstrate a simple and light-efficient way of generating non-diffracting Bessel beams for use in confocal microscopy. A number of imaging modalities using such beams is discussed. Preliminary experimental results... 详细信息

ISBN: (纸本)0819456756

关键词： confocal extended depth of focus Bessel beam fluorescence microscopy polarisation multi-photon

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Fast 3D blind deconvolution of even point spread functions

Fast 3D blind deconvolution of even point spread functions

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Yagle, AE Shah, S Univ Michigan Dept EECS Ann Arbor MI 48109 USA

ISBN: (纸本)0819452327

3-D mages obtained from optical sectioning microscopy are usually degraded by a point-spread function that is known to be an even function but is otherwise only approximately known, or even entirely unknown. We present a new algorithm for 3-D blind deconvolution of even point-spread functions that is both fast and (in the absence of noise) exact. Fourier transforms decouple the problem into 2-D, then 1-D blind deconvolution problems, greatly increasing computational speed. Numerical simulations demonstrate that the blind algorithm seems to perform both faster and more accurately than the non-blind iterative Lucy-Richardson algorithm.

关键词： Deconvolution Point spread functions 3D image processing Signal to noise ratio Convolution microscopy Fourier transforms Confocal microscopy image filtering Matrices

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The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes

The three-dimensional imaging of microspheres in confocal an...

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Yang, LS Taylor, CM Rakovich, Y McCabe, EM Univ Dublin Trinity Coll Dept Phys Dublin 2 Ireland

ISBN: (纸本)0819447641

images of the microspheres are studied in three-dimensions by using the confocal and conventional scanning polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.

关键词： three-dimensional microscopy polarization microsphere

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Depolarization by high-aperture focusing

Depolarization by high-aperture focusing

引用

conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Bahlmann, K Hell, SW MIT Dept Mech Engn Cambridge MA 02139 USA

ISBN: (纸本)0819443603

We propose and demonstrate a method employing ferroelectric monomolecular layers, by which it is possible to precisely measure the planar light field polarization in the focus of a lens. This method allowed us to establish for the first time to our knowledge, the perpendicularly oriented field that is anticipated at high apertures. For a numerical aperture 1.4 oil immersion lens illuminated with linearly polarized plane waves, the integral of the modulus square of the perpendicular component amounts to (1.51 (r)0.2)% of that of the initial polarization. It is experimentally proven that depolarization decreases with decreasing aperture angle and increases when using annular apertures. Annuli formed by a central obstruction with a diameter of 89% of that of the entrance pupil raise the integral to 5.5%. This compares well with the value of 5.8% predicted by electromagnetic focusing theory;however, the depolarization is also due to imperfections connected with focusing by refraction. Besides fluorescence microscopy and single molecule spectroscopy, the measured intensity of the depolarized component in the focal plane is relevant to all forms of light spectroscopy combining strong focusing with polarization analysis.

关键词： Luminescence Polarization Molecules Objectives microscopy Single molecule spectroscopy Refraction Electromagnetism Wavefronts Optical microscopy

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Three-dimensional state space realization algorithm: noise suppression of fluorescence microscopy images and point spread functions

Three-dimensional state space realization algorithm: noise s...

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Lai, XM Ward, ES Lin, ZP Ober, RJ Univ Texas SW Med Ctr Ctr Immunol Dallas TX 75235 USA

ISBN: (纸本)0819456756

A recently developed algorithm is applied to calculate a state space realization of a 3D microscopy image set. It is based on interpreting the image set as the impulse response of a 3D se arable system. As an application it is shown how this algorithm. combined with approximation steps, can be used to suppress noise in 3D experimental point spread functions. The approach was motivated by a well known problem that a noisy point spread function degrades the results of deconvolution algorithms for the restoration of 3D fluorescence microscopy image sets. The proposed approach can also be applied to 3D fluorescence microscopy image sets of cells.

关键词： point spread function estimation state space algorithm noise suppression

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Lateral resolution enhancement in confocal self-interference microscopy

Lateral resolution enhancement in confocal self-interference...

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Kang, D Gweon, D Korea Adv Inst Sci & Technol Med Engn Dept NOM Lab Taejon 305701 South Korea

ISBN: (纸本)0819456756

Lateral resolution enhancement in confocal self-interference microscopy (CSIM) is evaluated. CSIM, which uses the birefringence of the calcite plate to generate self-interference pattern, sharpens the central lobe of the effective spot. Numerical simulation results of two-dimensional imaging performances are presented. Two-point resolution of 149nm is achieved, which is enhanced by nearly 100% compared to that of confocal microscopy.

关键词： confocal microscopy superresolution birefringence self-interference

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High-throughput hyperspectral microscopy

High-throughput hyperspectral microscopy

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Gehm, M. E. Brady, D. J. Duke Univ Fitzpatrick Ctr Photon Durham NC 27708 USA

ISBN: (纸本)0819461326

A hyperspectral imager provides a 3-D data cube in which the spatial information (2-D) of the image is complemented by spectral information (1-D) about each spatial location. A static, high-throughput spectrometer design previously developed by our group can be used as the spectral engine in a high-throughput hyperspectral imager that avoids the Fourier undersampling issues present in previous dispersive designs. We present the theory for both pushbroom and tomographic operation and describe experimental results from our proof-of-concept implementation of a hyperspectral microscope.

关键词： hyperspectral imaging multidimensional microscopy

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image estimation accounting for point-spread function depth variation in three-dimensional fluorescence microscopy

Image estimation accounting for point-spread function depth ...

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Preza, C Conchello, JA Washington Univ Dept Elect Engn St Louis MO 63130 USA

ISBN: (纸本)0819447641

An approximate model for optical-sectioning microscopy describing depth-varying imaging is developed. The model incorporates changes in the point-spread function due to refractive index mismatch between the immersion medium and the specimen. which causes spherical aberration that worsens with increasing depth tinder the coverslip. Comparison of model predictions to measured images from a bead phantom shows that the approximate model captures the main features in the data. The model presented in this paper is the first step towards depth-variant image estimation for optical-sectioning microscopy. An expectation maximization algorithm for maximum-likelihood restoration based on this model is also presented.

关键词： 3D microscopy depth-variant imaging model maximum likelihood estimation

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