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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 559 条记录，以下是331-340 订阅

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Real time autonomous video image registration for endomicroscopy: Fighting the compromises

Real time autonomous video image registration for endomicros...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Vercauteren, Tom Meining, Alexander Lacombe, Francois Perchant, Aymeric Mauna Kea Technol 9 Rue Enghien Paris France INRIA Asclepios Res Grp Sophia Antipolis France TUM Klinikum Rechts Isar Munich Germany

ISBN: (纸本)9780819470362

Confocal endomicroscopy provides tools for in vivo imaging of human cell architecture endoscopically. These technologies are a tough challenge since multiple trade-offs have to be overcome: resolution versus field of view, dynamic versus stability, contrast versus low laser power or low contrast agent doses. Many difficult clinical applications, such as lung, bile duct, urethral imaging and NOTES applications, need to optimize miniaturization, resolution, frame rate and contrast agent dose simultaneously. We propose one solution based on real-time video image processing to efficiently address these trade-offs. Dynamic imaging provides a flow of images that we process in real time. images are aligned using efficient algorithms specifically adapted to confocal devices. From the displacement that we find across the images, instantaneous velocities are computed and used to compensate for motion distortions. All images are stitched together onto the same reference space and displayed in real-time to reconstruct an image of the entire surface explored during the clinical procedure. This representation brings both stability and an increased field of view. Moreover, because a given area can be imaged by several frames, the contrast can be improved using temporal adaptive averaging. Such processing enhances the visualization of the video sequence, overcoming most classical trade-offs. The stability and increased field of view help the clinician better focus his attention on his practice which improves the patient benefit. Our tools are currently evaluated in a multicenter clinical trial to assess the improvement of the clinical practice.

关键词： Cellvizio (R) microscopy mosaicing real-time

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Advantages of digital holographic microscopy for real-time full field absolute phase imaging

Advantages of digital holographic microscopy for real-time f...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Colomb, Tristan Charriere, Florian Kuehn, Jonas Marquet, Pierre Depeursinge, Christian CHUV Dept Psychiat Ctr Neurosci Psychiat Site Cery CH-1008 Prilly Switzerland Ecole Polytech Fed Lausanne Inst Imaging & Appl Opt CH-1015 Lausanne Switzerland

ISBN: (纸本)9780819470362

Different interferometric techniques were developed last decade to obtain full field, quantitative, and absolute phase imaging, such as phase-shifting, Fourier phase microscopy, Hilbert phase microscopy or digital holographic microscopy (DHM). Although, these techniques are very similar, DHM combines several advantages. In contrast, to phase shifting, DHM is indeed capable of single-shot hologram recording allowing a real-time absolute phase imaging. On the other hand, unlike to Fourier phase or Hilbert phase microscopy, DHM does not require to record in focus images of the specimen on the digital detector (CCD or CMOS camera), because a numerical focalization adjustment can be performed by a numerical wavefront propagation. Consequently, the depth of view of high NA microscope objectives is numerically extended. For example, two different biological cells, floating at different depths in a liquid, can be focalized numerically from the same digital hologram. Moreover, the numerical propagation associated to digital optics and automatic fitting procedures, permits vibrations insensitive full-field phase imaging and the complete compensation for a priori any image distortion or/and phase aberrations introduced for example by imperfections of holders or perfusion chamber. Examples of real-time full field phase images of biological cells have been demonstrated.

关键词： digital holography absolute phase measurement aberration compensation microscopy

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Resolution in 3D in multifocal plane microscopy

Resolution in 3D in multifocal plane microscopy

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Chao, Jerry Ram, Sripad Abraham, Anish V. Ward, E. Sally Ober, Raimund J. Univ Texas Dallas Dept Elect Engn Richardson TX 75083 USA Univ Texas Southwestern Med Ctr Dallas Dept Immunol Dallas TX 75390 USA Univ Texas Dallas Dept Comp Sci Richardson TX 75083 USA

ISBN: (纸本)9780819470362

Using single molecule microscopy, biological interactions can be imaged and studied at the level of individual biomolecules. When characterizing an imaged biological interaction, the distance separating the two participating biomolecules can provide valuable information. Therefore, the resolvability of an imaging setup is of practical significance in the analysis of the acquired image data. Importantly, the resolvability of the imaging setup needs evaluation in the 3D context, since in general biomolecules reside in 3D space within the cellular environment. We recently introduced an information-theoretic 2D resolution measure which shows that the resolution limit due to Rayleigh's criterion can be overcome. This new result predicts that the resolution of optical microscopes is not limited, but rather can be improved with increased photon counts detected from the single molecules. The 2D result was subsequently extended to the 3D context, and the proposed information-theoretic 3D resolution measure can readily be used to determine the resolvability of a conventional single focal plane imaging setup. Here, we consider the 3D resolution measure for a multifocal plane microscope setup, an imaging system which allows the concurrent imaging of multiple focal planes within a specimen. The technique is useful in applications such as the tracking of subcellular objects in 3D. By comparing their 3D resolution measures, we find a two-plane setup to outperform a comparable conventional single-plane setup in resolvability over a range of axial locations for the single molecule pair. Moreover, we investigate and compare the impact of noise on the resolvability of the two setups.

关键词： 3D resolution measure Fisher information matrix Cramer-Rao inequality multifocal plane microscopy

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Three-dimensional image study on the vascular structure after angiopoietin-1 transduction in isolated mouse pancreatic islets

Three-dimensional image study on the vascular structure afte...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： He, Jing Su, Dongming Trucco, Massimo Univ Pittsburgh Sch Med Dept Pediat Div ImmunogenetRangos Res Ctr 5th Ave 3460 Pittsburgh PA 15213 USA

ISBN: (纸本)9780819470362

Angiopoletin-1 (Ang-1) is essential for remodeling the primitive vascular plexus during embryonic development and for reducing plasma leakage in inflammation of adult vasculature. However, the role for Ang-1 in maintenance of vascular stability in isolated pancreatic islets is not fully understood. In this study, we compared the difference of vascular morphology between Ang-1 treated (n=5) and control mouse islets (n=5) using both two- and three-dimensional optical image analysis. Isolated mouse islets were transduced with Ang-1 or Lac Z (control) vector at 37 degrees C for 16 hours. Islets were incubated with both rat anti-CD31 antibody and rabbit anti-insulin antibody followed by incubation with Rhodamine-conjugated goat anti-rat IgG and Alexa-488 conjugated goat anti-rabbit IgG. Islets were viewed under a Nikon confocal microscope. Serial optical section images were captured and reconstructed using Nikon EZ-C1 software. Individual two-D and reconstructed three-D images were analyzed using MetaMorph image Analysis software. Islet vascular density was determined. In two-D images, there was no significant difference of vascular density between the two groups. The vascular morphology didn't show any obvious differences in two-D images either. However, in the three-D images, we found higher vascular density and more vascular branches in the Ang-1 transducted islets and vascular dilation in control group. In conclusion, using three-D image analysis, Ang-1 displayed functions in maintenance of vascular stability and in stimulating growth of vascular branches in isolated mouse pancreatic islets. In order to study further the regeneration of different cell contents in the spherical pancreatic islet, three-D image analysis is an effective method to approach this goal.

关键词： pancreatic islet three-D image two-D image 3D-reconstruction Ang-1 vascular structure confocal microscope mouse pancreas diabetics transplantation

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An optical technique for fast focusing applied to high-aperture microscopy

An optical technique for fast focusing applied to high-apert...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Botcherby, Edward Juskaitis, Rimas Booth, Martin Wilson, Tony Univ Oxford Dept Engn Sci Parks Rd Oxford OX1 3PJ England

In this paper we describe a simple method of optical refocusing for high numerical aperture imaging systems. As the first stage of this new method satisfies both the sine and Herschel conditions of geometric optics, i... 详细信息

ISBN: (纸本)9780819465566

关键词： optical refocusing sine condition Herschel condition confocal imaging

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A compact polarization converter to observe molecular orientation

A compact polarization converter to observe molecular orient...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Hashimoto, Mamoru Yoshiki, Keisuke Kanamaru, Ryosuke Hashimoto, Nobuyuki Araki, Tsutomu Osaka Univ Grad Sch Engn Sci 1-3 Machikaneyama Toyonaka Osaka 5608531 Japan Citizen Watch Co Ltd MHT R&D Dept Tokorozawa Saitama 3598511 Japan

ISBN: (纸本)9780819465566

We developed a compact polarization-converter using two liquid-crystal spatial-light-modulators with eight electrodes. The converter converted a linearly polarized beam to two orthogonal linearly polarized beams and a radially polarized beam, and the direction of the electric filed at the focal point were controlled three-dimensionally. We constructed a second-harmonic-generation microscope using the polarization-converter to observe three-dimensional molecular orientation and demonstrated the detectability of molecular orientation.

关键词： molecular orientation nonlinear microscopy second-harmonics-generation pupil filtering radially polarized beam

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Effects of specimen morphology on adaptive confocal and multiphoton microscopy

Effects of specimen morphology on adaptive confocal and mult...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Booth, Martin J. Wilson, Tony Univ Oxford Dept Engn Sci Parks Rd Oxford OX1 3PJ England

ISBN: (纸本)9780819465566

microscopy of thick biological specimens is often detrimentally affected by specimen-induced aberrations. In the simplest case, these aberrations arise from a refractive index mismatch between the immersion and mounting media. In other situations, the aberrations arise from variations in refractive index within the specimen. These aberrations cause loss of signal and reduced resolution. Aberrations can be corrected using adaptive optics, where a deformable mirror introduces equal but opposite aberrations into the optical path. Aberration correction can be performed by reconfiguring the deformable mirror and using the fluorescence signal as feedback, effectively maximising the fluorescence intensity. However, the degree to which aberrations affect the intensity is related to the distribution of fluorescence in the specimen. Signals from point-like objects are affected more than equivalent signals from from planar or volume objects. We investigate this effect and discuss the implications for adaptive optical microscopy of biological specimens.

关键词： confocal microscopy multiphoton microscopy aberrations adaptive optics

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Confocal Fluorescence lifetime imaging microscopy based on a realtime sampling method

Confocal Fluorescence lifetime imaging microscopy based on a...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Bae, Yoon. S. Lee, Dongsoo Moon, Sucbei Won, Young Jae Kim, Dug Y. Gwangju Inst Sci & Technol Dept Informat & Commun 1 Oryong Dong Kwangju 500712 South Korea

ISBN: (纸本)9780819465566

We present a fluorescence lifetime imaging microscope (FLIM) based on a real-time waveform acquisition method. The fluorophores were excited by a 635-mn gain-switched laser diode,, which produced short pulses with duration similar to 50 ps in a 20-MEz repetition rate. The fluorescence signals were detected by a silicon avalanche photo-diode (APD) in addition to a wide-band electric amplifier. The converted electric pulses were sampled by a high-speed digitizer of which sampling rate was 2 GS/s. In order to reduce the sampling interval for analyzing sub-nanosecond lifetimes, an interleaved data acquisition technique was used. The effective sampling rate was increased to 10 GS/s. In addition, the impulse response was measured simultaneously with the lifetime signals by an interleaving manner and was used in calibration of the system. By using these methods, accurate lifetime information was acquired in a short time less than 8 mu s.

关键词： confocal microscopy fluorescence lifetime imaging microscopy interleaved data acquisition

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Fluorescence lifetime imaging using light emitting diodes

Fluorescence lifetime imaging using light emitting diodes

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Kennedy, Gordon T. Elson, Daniel S. Munro, Ian Poher, Vincent French, Paul M. W. Neil, Mark A. A. Imperial Coll London Blackett Lab S Kensington Campus London SW7 2AZ England Imperial Coll London Dept Bioengn London SW7 2AZ England

We demonstrate fluorescence lifetime imaging using cheap, high power light emitting diodes as excitation sources. Both time domain and frequency domain fluorescence lifetime imaging techniques have been implemented at... 详细信息

ISBN: (纸本)9780819465566

关键词： fluorescence lifetime imaging LED microscopy

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High-resolution laser scanning microscopy with saturated excitation of fluorescence

High-resolution laser scanning microscopy with saturated exc...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Fujita, Katsumasa Kawano, Shogo Kobayashi, Minoru Kawata, Satoshi Osaka Univ Dept Appl Phys 2-2 Yamadaoka Suita Osaka 5650871 Japan Osaka Univ Dept Frontier Biosci Suita Osaka 5650871 Japan Nanophoton Corp Suita Osaka 5650871 Japan RIKEN Wako Saitama 3510198 Japan

ISBN: (纸本)9780819465566

We propose the use of saturated excitation to improve the spatial resolution of a laser scanning confocal fluorescence microscope. The saturated excitation induces nonlinear response in fluorescence emission that gives higher-order spatial frequency components in the point spread fuction of the microscope. To extract the nonlinear responses in fluorescence emission, we modulate the excitation intensity temporally at a single frequency (omega) and demodulate the fluorescence signal at the harmonic frequencies (2 omega, 3 omega,...). We found that the fluorescence signal demodulated at nth harmonic frequency is proportional to nth power of the excitation intensity, where n-fold improvement of the spatial resolution can be achieved. We experimentally confirmed that the demodulated signal at the second harmonic frequency was proportional to the square of the excitation intensity. The improvement of spatial resolution was also confirmed by observing a fluorescent sample.

关键词： laser scanning microscopy confocal microscopy fluorescence saturated excitation high-resolution microscopy

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