Polarization control and analysis have proven to be a powerful tool both in conventional and confocal microscopies, but most of these approaches have used homogeneously polarized pupils. Other groups have had varying ...
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ISBN:
(纸本)081943535X
Polarization control and analysis have proven to be a powerful tool both in conventional and confocal microscopies, but most of these approaches have used homogeneously polarized pupils. Other groups have had varying levels of success producing beams with spatially inhomogeneous polarization. In this paper, we describe the generation and focusing properties of such beams and their application to scanning laser microscopy. We specifically consider microscopy using the lowest-order azimuthally polarized beam.
In this work, we are proposing the use of digital image enhancement in three dimensional (3D) Integral Imaging (InI) applying in small objects visualization. We used a well known image filter algorithm for enhance the...
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ISBN:
(纸本)0819461326
In this work, we are proposing the use of digital image enhancement in three dimensional (3D) Integral Imaging (InI) applying in small objects visualization. We used a well known image filter algorithm for enhance the edges and detail information of the 3D reconstruction InI image via unsharp masking. Small objects as bugs were recorded in an elemental image array, image processed and digital reconstructed as 3D objects. We implemented the algorithm over the elemental image array as usual and using an innovative technique that involves a simple digital reconstruction algorithm by quadruple pixel extraction. Digital results show an improvement in details visualization, which have potential application in 3D microscopy.
Few models, based on the diffraction theory, are proposed in order to evaluate the point spread function of different microscope objectives used in a digital holographic microscope. Because in holography the phase inf...
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ISBN:
(纸本)0819452327
Few models, based on the diffraction theory, are proposed in order to evaluate the point spread function of different microscope objectives used in a digital holographic microscope. Because in holography the phase information is essential, a 3D amplitude point spread function (APSF), modulus and phase, is necessary, in order to properly deconvolute the 3D images obtained. Scalar Debye theory, paraxial approximation and vectorial Debye theory are used to solve the diffraction problem and the theoretical predicted 3D APSFs obtained. with these models are compared.
We report a novel microscopy technique, utilizing our previously reported expanded point information content (EPIC) concept [1], to extend the technique into the coherent regime. Preliminary data shows coherent EPIC (...
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ISBN:
(数字)9781510605824
ISBN:
(纸本)9781510605817;9781510605824
We report a novel microscopy technique, utilizing our previously reported expanded point information content (EPIC) concept [1], to extend the technique into the coherent regime. Preliminary data shows coherent EPIC (CoEPIC) can image reflective samples successfully, and can recover the 3D structure without the need to acquire an image stack at multiple depths. Numerical simulation demonstrates the potential super-resolution capability of CoEPIC.
We present an easy-to-use multi-immersion open-top light-sheet microscope designed specifically for high-throughput imaging of a diverse set of tissues prepared with a variety of clearing protocols.
ISBN:
(纸本)9781510632547
We present an easy-to-use multi-immersion open-top light-sheet microscope designed specifically for high-throughput imaging of a diverse set of tissues prepared with a variety of clearing protocols.
The optical resolution limits the observation of fine features with size below diffraction limit, e.g. 500nm. We propose and demonstrate a technique that uses collimated laser scattering to characterize such features....
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ISBN:
(纸本)0819452327
The optical resolution limits the observation of fine features with size below diffraction limit, e.g. 500nm. We propose and demonstrate a technique that uses collimated laser scattering to characterize such features. We use a new illumination technique that let us image bio-molecules down to 50nm with optical microscopy. We observed vesicles with size down to 50nm and visualize the flow of such tiny molecules in the live cells. We could also characterize the detailed size and molecular weight of such particles.
We review several quantitative phase imaging methods that retrieve phase from a through-focus series of intensity images (specifically, iterative methods and Transport of Intensity) and compare their performance when ...
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ISBN:
(纸本)9780819493583
We review several quantitative phase imaging methods that retrieve phase from a through-focus series of intensity images (specifically, iterative methods and Transport of Intensity) and compare their performance when using partially coherent illumination, such as in the case in a brightfield microscope or X-ray imaging system.
A system for quantification of neurite outgrowth in in-vitro experiments is described. The system is developed for routine use in a high-throughput setting and is therefore needs fast, cheap, and robust. It relies on ...
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ISBN:
(纸本)0819452327
A system for quantification of neurite outgrowth in in-vitro experiments is described. The system is developed for routine use in a high-throughput setting and is therefore needs fast, cheap, and robust. It relies on automated digital microscopical imaging of microtiter plates. image analysis is applied to extract features for characterisation of neurite outgrowth. The system is tested in a dose-response experiment on PC12 cells + Taxol. The performance of the system and its ability to measure changes on neuronal morphology is studied.
We describe a method of obtaining optically sectioned fluorescence images in a widefield conventional microscope by interfering two beams on an object so as to illuminate it with a single spatial frequency fringe patt...
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ISBN:
(纸本)0819427004
We describe a method of obtaining optically sectioned fluorescence images in a widefield conventional microscope by interfering two beams on an object so as to illuminate it with a single spatial frequency fringe pattern. images taken at three spatial positions of the fringe pattern are processed in real time to produce optically sectioned images which are substantially similar to those obtained with confocal microscopes.
We describe a simple modification to a rigid endoscope so as to provide both high quality conventional endoscopic as well as and confocal endoscopic images of reasonably accessible regions of the body in real time. Th...
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ISBN:
(纸本)0819447641
We describe a simple modification to a rigid endoscope so as to provide both high quality conventional endoscopic as well as and confocal endoscopic images of reasonably accessible regions of the body in real time. The systems are based around either host lenslet-array tandem scanning microscope together with laser illumination or a structured illumination approach together with a conventional incoherent illumination source. images taken in fluorescence are presented using this combined conventional and confocal endoscope.
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