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conference on Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine ix

作者： Jenkins, MW Rothenberg, F Roy, D Nikolski, VP Wilson, DL Efimov, IR Rollins, AM Case Western Reserve Univ Dept Biomed Engn Cleveland OH 44106 USA

ISBN: (纸本)0819456640

Computed tomography (CT), ultrasound, and magnetic resonance imaging have been used to image and diagnose diseases of the human heart. By gating the acquisition of the images to the heart cycle (gated imaging), these modalities enable one to produce 3D images of the heart without significant motion artifact and to more accurately calculate various parameters such as ejection fractions [1-3]. Unfortunately, these imaging modalities give inadequate resolution when investigating embryonic development in animal models. Defects in developmental mechanisms during embryogenesis have long been thought to result in congenital cardiac anomalies. Our understanding of normal mechanisms of heart development and how abnormalities can lead to defects has been hampered by our inability to detect anatomic and physiologic changes in these small (<2mm) organs. Optical coherence tomography (OCT) has made it possible to visualize internal structures of the living embryonic heart with high-resolution in two- and threedimensions. OCT offers higher resolution than ultrasound (30 um axial, 90 um lateral) and magnetic resonance microscopy (25 um axial, 31 um lateral) [4, 5], with greater depth penetration over confocal microscopy (200 um). Optical coherence tomography (OCT) uses back reflected light from a sample to create an image with axial resolutions ranging from 2-15 um, while penetrating 1-2 mm in depth [6]. In the past, OCT groups estimated ejection fractions using 2D images in a Xenopus laevis [7], created 3D renderings of chick embryo hearts [8], and used a gated reconstruction technique to produce 2D Doppler OCT image of an in vivo Xenopus laevis heart [9]. In this paper we present a gated imaging system that allowed us to produce a 16-frame 3D movie of a beating chick embryo heart. The heart was excised from a day two (stage 13) chicken embryo and electrically paced at 1 Hz. We acquired 2D images (B-scans) in 62.5 ms, which provides enough temporal resolution to distinguish end-c

关键词： Heart Optical coherence tomography 3D image processing Gated imaging 3D acquisition 3D image reconstruction 3D metrology Visualization Computed tomography Ultrasonography

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Fluorescence lifetime imaging microscopy based on a synchroscan streak camera

Fluorescence lifetime imaging microscopy based on a synchros...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Qu, J Li, J Niu, HB Shenzhen Univ Inst Optoelect Shenzhen 518060 Guangdong Prov Peoples R China

ISBN: (纸本)0819452327

A new time-domain two-dimensional fluorescence lifetime detection method and a fluorescence lifetime imaging microscope that is based on a synchroscan streak camera are presented. This system can generate in parallel a complete two-dimensional fluorescence lifetime image without scanning the light. We demonstrate the acquisition of a fluorescence lifetime image of a semiconductor ET material sample at a temporal resolution of 2ps and spatial resolution of 4mum within 40ms.

关键词： fluorescence lifetime fluorescence lifetime imaging fluorescence lifetime imaging microscopy synchroscan scan streak camera temporal resolution spatial resolution

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Optimised reflection imaging for surface roughness analysis using confocal laser scanning microscopy and height encoded image processing

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Journal of Physics: conference Series 2005年第1期13卷

作者： S J Tomovich Z Peng School of Engineering James Cook University Townsville Qld 4811 Australia

Quantitative surface measurement is an important field in engineering. Due to the complexity of surface topography, accurate surface characterisation requires three-dimensional (3D) surface measurements not provided by many current measurement systems. A nondestructive and versatile technique for quantifying 3D surface features is Confocal Laser Scanning microscopy (CLSM). However, there is little documentation on standard CLSM hardware settings required to capture images of suitable quality for 3D surface measurements. Understanding the complex relationship between CLSM settings, specimen properties and image quality is crucial to optimising the acquisition process for quantitative 3D surface measurements. The response of image quality to variations in CLSM hardware settings and specimen properties has been investigated in the study. Through the investigations, criteria have been developed to select optimal CLSM hardware settings to minimise image noise, eliminate image distortion, maximise contrast and resolution for reliable and accurate 3D numerical surface measurements. A reliable 3D image analysis system has been developed for image processing and surface measurement of engineering surfaces and small particles. The image analysis system developed in Matlab for the confocal system provides a new means to quantitatively characterise a wide range of engineering surfaces with accuracy and efficiency.

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Three-dimensional imagery using multiple nanometric probes

Three-dimensional imagery using multiple nanometric probes

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Moneron, G Dubois, A Boccara, AC Ecole Super Phys & Chim Ind Ville Paris Lab Opt Phys CNRS UPR A0005 F-75231 Paris 5 France

ISBN: (纸本)0819452327

We present a high-resolution imaging technique using nanometric beads as multiple scattering local probes. The positions of the beads are determined in three dimensions by white-light interference microscopy. The technique has been applied to study the deformation of gels under mechanical constraint. The location of Brownian moving beads has also been demonstrated with nanometer spatial precision and 10 mus acquisition time. High-resolution 3-D imaging of hollow structures explored by the beads in relatively transparent materials should be possible.

关键词： interference microscopy three-dimensional microscopy scanning probe microscopy

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Fast 3D blind deconvolution of even point spread functions

Fast 3D blind deconvolution of even point spread functions

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Yagle, AE Shah, S Univ Michigan Dept EECS Ann Arbor MI 48109 USA

ISBN: (纸本)0819452327

3-D mages obtained from optical sectioning microscopy are usually degraded by a point-spread function that is known to be an even function but is otherwise only approximately known, or even entirely unknown. We present a new algorithm for 3-D blind deconvolution of even point-spread functions that is both fast and (in the absence of noise) exact. Fourier transforms decouple the problem into 2-D, then 1-D blind deconvolution problems, greatly increasing computational speed. Numerical simulations demonstrate that the blind algorithm seems to perform both faster and more accurately than the non-blind iterative Lucy-Richardson algorithm.

关键词： Deconvolution Point spread functions 3D image processing Signal to noise ratio Convolution microscopy Fourier transforms Confocal microscopy image filtering Matrices

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Automatic quantification of neurite outgrowth by means of image analysis

Automatic quantification of neurite outgrowth by means of im...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Van de Wouwer, G Nuydens, R Meert, T Weyn, B Univ Antwerp Visielab Dept Phys B-2020 Antwerp Belgium

ISBN: (纸本)0819452327

A system for quantification of neurite outgrowth in in-vitro experiments is described. The system is developed for routine use in a high-throughput setting and is therefore needs fast, cheap, and robust. It relies on automated digital microscopical imaging of microtiter plates. image analysis is applied to extract features for characterisation of neurite outgrowth. The system is tested in a dose-response experiment on PC12 cells + Taxol. The performance of the system and its ability to measure changes on neuronal morphology is studied.

关键词： automation microscopy image analysis neurite outgrowth

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Point spread function models for digital holographic microscopy

Point spread function models for digital holographic microsc...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Marian, A Depeursinge, C Swiss Fed Inst Technol Inst Biomed Imaging Opt & Engn CH-1015 Lausanne Switzerland

ISBN: (纸本)0819452327

Few models, based on the diffraction theory, are proposed in order to evaluate the point spread function of different microscope objectives used in a digital holographic microscope. Because in holography the phase information is essential, a 3D amplitude point spread function (APSF), modulus and phase, is necessary, in order to properly deconvolute the 3D images obtained. Scalar Debye theory, paraxial approximation and vectorial Debye theory are used to solve the diffraction problem and the theoretical predicted 3D APSFs obtained. with these models are compared.

关键词： point spread function diffraction digital holographic microscopy

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Nano-scale optical imaging technique

Nano-scale optical imaging technique

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Wu, S Tang, YC PEER Ctr Covina CA 91722 USA

ISBN: (纸本)0819452327

The optical resolution limits the observation of fine features with size below diffraction limit, e.g. 500nm. We propose and demonstrate a technique that uses collimated laser scattering to characterize such features. We use a new illumination technique that let us image bio-molecules down to 50nm with optical microscopy. We observed vesicles with size down to 50nm and visualize the flow of such tiny molecules in the live cells. We could also characterize the detailed size and molecular weight of such particles.

关键词： single particle laser scattering microscopy

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Real-time digital design for an optical coherence tomography acquisition and processing system

Real-time digital design for an optical coherence tomography...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Ralston, TS Mayen, JA Marks, D Boppart, SA Univ Illinois Beckman Inst Adv Sci & Technol Urbana IL 61801 USA

ISBN: (纸本)0819452327

We present a real-time, multi-dimensional, digital, optical coherence tomography (OCT) acquisition and imaging system. The system consists of conventional OCT optics, a rapid scanning optical delay (RSOD) line to support fast data acquisition rates, and a high-speed A/D converter for sampling the interference waveforms. A 1M-gate Virtex-II field programmable gate array (FPGA) is designed to perform digital down conversion. This is analogous to demodulating and low-pass filtering the continuous time signal. The system creates in-phase and quadrature-phase components using a tunable quadrature mixer. Multistage polyphase finite impulse response (FIR) filtering and down sampling is used to remove unneeded high frequencies. A floating-point digital signal processor (DSP) computes the magnitude and phase shifts. The data is read by a host machine and displayed on screen at real-time rates commensurate with the data acquisition rate. This system offers flexible acquisition and processing parameters for a wide range of multi-dimensional optical microscopy techniques.

关键词： digital signal processing optical coherence tomography Doppler spectroscopic real-time

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A phase-shifting DIC technique for measuring 3D phase objects: Experimental verification

A phase-shifting DIC technique for measuring 3D phase object...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： King, SV Cogswell, CJ Univ Colorado Dept Elect & Comp Engn Boulder CO 80309 USA

ISBN: (纸本)0819452327

Experimental verification of our previously proposed linear phase imaging technique for differential interference contrast microscopy (DIC) microscopy is presented. This technique first applies phase-shifting methods to DIC to acquire linear phase gradient images in two orthogonal directions. A special Fourier integration algorithm is then applied to the combined phase gradient images to create a single linear phase image in which intensity is proportional to phase. This overcomes the limitations of traditional DIC, which cannot accurately measure the phase (i.e. refractive index or thickness) of embedded 3D phase objects. The linear phase imaging technique is implemented using a standard DIC microscope altered to allow controlled phase shifting, a low noise CCD camera, and post-processing in Matlab. The results presented confirm the linear proportionality of intensity to phase in these images.

关键词： DIC phase phase gradient phase imaging spiral phase interference microscopy

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