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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 559 条记录，以下是461-470 订阅

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Scanning aperture polarized light microscope: observation of small calcite crystals using oblique illumination

Scanning aperture polarized light microscope: observation of...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Shribak, M Oldenbourg, R Marine Biol Lab Woods Hole MA 02543 USA

ISBN: (纸本)0819443603

The article presents a new design of a polarization microscope with a scanning liquid crystal aperture. The scanning device is based on the earlier reported Pol-Scope technique and includes a liquid crystal universal compensator. It is mounted in the front focal plane of the high numerical aperture condenser lens on the microsccope. By occluding different portions of the aperture, an oblique beam of variable tilt angle and azimuth is created for illuminating the specimen. Birefringence measurements are recorded for different mask configurations and results are evaluated to determine the retardance magnitude, azimuth and direction of optic axis of the specimen. We report measurements using small calcite crystals that confirm our theoretical predictions.

关键词： polarization microscopy conoscopy birefringence crystal optic

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Source coherence and optical sectioning strength in direct-view microscopy

Source coherence and optical sectioning strength in direct-v...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Taylor, CM Yang, L McCabe, EM Univ Dublin Trinity Coll Dept Phys Dublin 2 Ireland

ISBN: (纸本)0819443603

The direct-view microscope is a confocal microscope which allows faster image acquisition rates than typical confocal scanning optical microscopes through the use of a pinhole array rather than the usual single pinhole. We present a theoretical investigation of the effects of source coherence on optical sectioning in direct-view microscopy. As a first step we present an equation which describes the optical sectioning strength of a coherent source brightfield DVM employing an infinite pinhole array. By simulation of both this and the finite array equation which show the existence of certain 'principal' sidelobes which are likely to represent the most problematic artifact of coherent source imaging. By further analysis of the infinite Arran, equation, we arrive at an expression which describes the defocus positions where the principal sidelobes occur. Finally, we move on to show how rectangular arrays are predicted, by the infinite array equation, to outperform square arrays and we show examples of this.

关键词： optical sectioning source coherence Real-Time imaging direct-niew microscopy Confocal microscopy

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Application of phase retrieved pupil functions in wide-field fluorescence microscopy

Application of phase retrieved pupil functions in wide-field...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Hanser, BM Gustafsson, MGL Agard, DA Sedat, JW Univ Calif San Francisco Grad Grp Biophys San Francisco CA 94143 USA

ISBN: (纸本)0819443603

multidimensional fluorescence microscopy data are often collected under imaging conditions that cause aberrations. For proper deconvolution of such data, one needs to generate a Point Spread Function (PSF) that includes the aberrations. Even if it is possible to model the imaging aberrations theoretically, there remains the problem of modifying the PSF accordingly. Measured PSFs are difficult to modify, and theoretical PSFs lack lens- and system-specific features. Phase retrieval approaches can be used to produce a compact and modifiable description of a microscope system, including its measured, system-specific performance. These pupil functions can be used in a depth-dependent deconvolution to correct for geometric distortions and improve restoration of relative intensities compared to spatially invariant deconvolution using unaberrated PSFs.

关键词： phase retrieval Point Spread Function (PSF) wide-field microscopy fluorescence microscopy Optical Transfer Function (OTF) phase diversity optical microscopy pupil function depth aberrations spherical aberrations

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Proceedings of SPIE: Three-dimensional and multidimensional microscopy: image acquisition and processing ix

Proceedings of SPIE: Three-dimensional and Multidimensional ...

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Three-dimensional and multidimensional microscopy: image acquisition and processing ix

This Volume 4621 of the conference proceedings contains 16 papers. Topics discussed include three dimensional and multidimensional microscopy, new methods and instruments, microscope characterization and assessment an... 详细信息

关键词： Microscopic examination

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Modeling ECM fiber formation: Structure information extracted by analysis of 2D and 3D image sets

Modeling ECM fiber formation: Structure information extracte...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Wu, J Voytik-Harbin, SL Filmer, DL Hoffmann, CM Yuan, B Chiang, CS Sturgis, J Robinson, JP Purdue Univ Sch Vet Med Dept BMS W Lafayette IN 47907 USA

ISBN: (纸本)0819443603

Recent evidence supports the notion that biological functions of extracellular matrix (ECM) are highly correlated to its structure. Understanding this fibrous structure is very crucial in tissue engineering to develop the next generation of biomaterials for restoration of tissues and organs. In this paper, we integrate confocal microscopy imaging and image-processing techniques to analyze the structural properties of ECM. We describe a 2D fiber middle-line tracing algorithm and apply it via Euclidean distance maps (EDM) to extract accurate fibrous structure information, such as fiber diameter, length, orientation, and density, from single slices. Based on a 2D tracing algorithm, we extend our analysis to 3D tracing via Euclidean distance maps to extract 3D fibrous structure information. We use computer simulation to construct the 3D fibrous structure which is subsequently used to test our tracing algorithms. After further image processing, these models are then applied to a variety of ECM constructions from which results of 2D and 3D traces are statistically analyzed.

关键词： extracellular matrix (ECM) 3D structure model tracing software computer simulation confocal microscopy

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High resolution 3D-reconstruction from serial sections - Microscope instrumentation, software design and its implementations

High resolution 3D-reconstruction from serial sections - Mic...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Verbeek, FJ Boon, PJ Netherlands Inst Dev Biol Hubrecht Lab NL-3584 CT Utrecht Netherlands

ISBN: (纸本)0819443603

This paper describes a set-up for high-resolution imaging with a conventional microscope that allows producing a 3D-image set from which a 3D model can be derived. The 3D-image set, is de facto, a gray value voxel model. In order to obtain such 3D image set a good administration needs to be maintained at acquisition and moreover, the acquisition must be realized in two steps. High-resolution images are built of image tiles and sophisticated algorithms are required to build a coherent image from the tiles.

关键词： image processing high-resolution image image tile image mosaic 3D reconstruction 3D image bioinformatics

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Optical properties of spherical microlens

Optical properties of spherical microlens

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Volkov, UP Avetisyan, UA Baibyrin, VB Bespalova, NV Saratov State Tech Univ Saratov 410007 Russia

ISBN: (纸本)0819443603

A scanning optical far and near-field microscopes resolution is limited by focused light spot size used to scan a sample. As a focusing element in scanning microscopes are ordinary used lenses with dimensions of rather more than a light wavelength. The focus spot size of such lens is limited by well-known diffraction limit. However, if a lens dimension is comparable to a light wavelength the Fresnel's diffraction formula of focus spot size calculation is not suitable. At present, there are no data about theoretical or experimental studies of the small lenses and even about the ones fabrication methods. This paper is considers the fabrication method of small spherical glass lenses with diameters from I um to 100 um and discusses some theoretical approaches to the focus spot size calculation and it experimental measurement. The lenses fabrication method is based on melting of fine glass powder with oxygen-acetylene burner with subsequent lenses selection under conventional light microscope. To calculate a spot size of the small lens the Mie theory of light scattering in near field by small spherical particles has been used. The light intensity diagrams of sphere lens of various diameters calculated by developed program are presented.

关键词： spherical microlens scanning optical microscopy Mie theory optic

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High resolution spectral self-interference fluorescence microscopy

High resolution spectral self-interference fluorescence micr...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Swan, AK Moiseev, L Tong, YJ Lipoff, SH Karl, WC Goldberg, BB Ünlü, MS Boston Univ Dept Elect & Comp Engn Boston MA 02215 USA

ISBN: (纸本)0819443603

We present a new method of fluorescence imaging, which yields nm-scale axial height determination and similar to15 nm axial resolution. The method uses the unique spectral signature of the fluorescent emission intensity well above a reflecting surface to determine vertical position unambiguously. We have demonstrated axial height determination with nm sensitivity by resolving the height difference of fluorescein directly on the surface or ontop of streptavidin. While different positions of fluorophores of different color are determined independently with nm precision, resolving the position of two fluorophores of the same color is a more convoluted problem due to the finite spectral emission widow of the fluorophores. Hence, for physically close (

关键词： fluorescence microscopy self interference spectroscopy

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Mapping organism expression levels at cellular resolution in developing Drosophila

Mapping organism expression levels at cellular resolution in...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Knowles, DW Keränen, S Biggin, MD Sudar, D Lawrence Berkeley Lab Dept Cell & Mol Biol Berkeley CA 94720 USA

ISBN: (纸本)0819443603

The development of an animal embryo is orchestrated by a network of genetically determined, temporal and spatial gene expression patterns that determine the animals final form. To understand such networks, we are developing novel quantitative optical imaging techniques to map gene expression levels at cellular and sub-cellular resolution within pregastrula Drosophila. Embryos at different stages of development are labeled for total DNA and specific gene products using different fluorophors and imaged in 3D with confocal microscopy. Innovative steps have been made which allow the DNA-image to be automatically segmented to produce a morphological mask of the individual nuclear boundaries. For each stage of development an "average" morphology is chosen to which images from different embryo are compared. The morphological mask is then used to quantify gene-product on a per nuclei basis. What results is an atlas of the relative amount of the specific gene product expressed within the nucleus of every cell in the embryo at the various stages of development. We are creating a quantitative database of transcription factor and target gene expression patterns in wild-type and factor mutant embryos with single cell resolution. Our goal is to uncover the rules determining how patterns of gene expression are generated.

关键词： confocal imaging image segmentation image registration quantitative fluorescence analysis regulatory gene networks Drosophila

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Depolarization by high-aperture focusing

Depolarization by high-aperture focusing

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Bahlmann, K Hell, SW MIT Dept Mech Engn Cambridge MA 02139 USA

ISBN: (纸本)0819443603

We propose and demonstrate a method employing ferroelectric monomolecular layers, by which it is possible to precisely measure the planar light field polarization in the focus of a lens. This method allowed us to establish for the first time to our knowledge, the perpendicularly oriented field that is anticipated at high apertures. For a numerical aperture 1.4 oil immersion lens illuminated with linearly polarized plane waves, the integral of the modulus square of the perpendicular component amounts to (1.51 (r)0.2)% of that of the initial polarization. It is experimentally proven that depolarization decreases with decreasing aperture angle and increases when using annular apertures. Annuli formed by a central obstruction with a diameter of 89% of that of the entrance pupil raise the integral to 5.5%. This compares well with the value of 5.8% predicted by electromagnetic focusing theory;however, the depolarization is also due to imperfections connected with focusing by refraction. Besides fluorescence microscopy and single molecule spectroscopy, the measured intensity of the depolarized component in the focal plane is relevant to all forms of light spectroscopy combining strong focusing with polarization analysis.

关键词： Luminescence Polarization Molecules Objectives microscopy Single molecule spectroscopy Refraction Electromagnetism Wavefronts Optical microscopy

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