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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

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On-line 3-dimensional confocal imaging in vivo

On-line 3-dimensional confocal imaging in vivo

引用

Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)

作者： Li, J. Jester, J.V. Cavanagh, H.D. Petroll, W.M. Univ of Texas Southwestern Medical Cent Dallas United States

ISBN: (纸本)0780356756

Tandem Scanning Confocal microscopy (TSCM) is ideal for in vivo corneal imaging, but currently requires time-consuming off-line image processing. To overcome this limitation, we have developed a novel PC-based TSCM system. The system is the first to provide interactive 3-D on-line image acquisition and analysis of in vivo confocal images.

关键词： Medical imaging

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Multi-photon excitation fluorescence imaging microscopy in the biomedical sciences

引用

conference on Optical Diagnostics of Living Cells II

作者： Periasamy, A Univ Virginia Dept Biol Ctr Cellular Imaging Charlottesville VA 22903 USA

ISBN: (纸本)0819430749

Modem research in molecular, cellular, and developmental biology requires the precise measurement of cellular or subcellular activity in two- or three-dimensions. Many available fluorescence microscope techniques are yielding limited detail about the organization and dynamics of complex cellular structures. But the multiphoton (two- or more photon) excitation fluorescence imaging microscopy (MEFIM) system proposed here will provide a unique, state-of-the-art opportunity to integrate advances in optical microscopy, low-light video detection, and two- or three-dimensional image analysis in measuring the fluorescent signals from living cells. The MEFIM system uses longer excitation wavelength, which increases the penetration of the excitation of the sample, yet essentially reduces photobleaching and autofluorescence. But, more importantly, the infrared light excitation in the MEFIM techniques allows maintaining cell viability for longer periods of time and thus acquisition of more images. In our current work, we integrated the MEFIM system with one-photon laser scanning confocal microscope coupled to a verdi pumped tunable femtosecond pulsed ti-sapphire laser. Appropriate wavelength was tuned to excite the fluorescently labeled specimens and MEFIM images were acquired from living biological specimens at different optical sections. The importance of the MEFIM image acquisition and processing for biomedical sciences are discussed.

关键词： multi-photon (MP) excitation two-photon (2p) excitation three-photon (3p) excitation fluorescence microscopy developmental biology bleomycin focal adhesion proteins and fluorescence resonance energy transfer (FRET)

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On-line 3-dimensional confocal imaging in vivo

On-line 3-dimensional confocal imaging in vivo

引用

Annual International conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

作者： J. Li J.V. Jester H.D. Cavanagh W.M. Petroll University of Texas Southwestern Medical Centre Dallas TX USA Department of Opthamology University of Texas Southwestern Medical Centre Dallas TX USA

Tandem Scanning Confocal microscopy (TSCM) is ideal for in vivo corneal imaging, but currently requires time-consuming off-line image processing. To overcome this limitation, we have developed a novel PC-based TSCM system. The system is the first to provide interactive 3-D online image acquisition and analysis of in vivo confocal images.

关键词： In vivo Optical imaging Biomedical imaging Pixel Cornea microscopy Optical feedback Lenses Biomedical optical imaging image processing

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Optically sectioned images in widefield fluorescence microscopy

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Wilson, T Neil, MAA Juskaitis, R Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

An analysis is presented of the image formation in widefield fluorescence microscopy using standard light. The region of support of the resulting optical transfer function is discussed.

ISBN: (纸本)0819427004

An analysis is presented of the image formation in widefield fluorescence microscopy using standard light. The region of support of the resulting optical transfer function is discussed.

关键词： Luminescence microscopy Fringe analysis Spatial frequencies image acquisition Microscopes Imaging systems Optical transfer functions Point spread functions Roads

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image enhancement for a low cost TEM acquisition system.

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Randall, G Fernandez, A Trujillo-Cenoz, O Apelbaum, G Bertalmio, M Vazquez, L Malmierca, F Morelli, P Univ Republ Fac Ingn Inst Ingn Elect Montevideo Uruguay

This paper describes a method for the improvement of biological images acquired using a Transmission Electronic Microscope (TEM). Several techniques are presented that deal with noise reduction, artifact removal and n... 详细信息

ISBN: (纸本)0819427004

关键词： Transmission Electronic microscopy image processing non-uniform illumination

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microscopy using an interference pattern as illumination source

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Juskaitis, R Neil, MAA Wilson, T Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

ISBN: (纸本)0819427004

We describe a method of obtaining optically sectioned fluorescence images in a widefield conventional microscope by interfering two beams on an object so as to illuminate it with a single spatial frequency fringe pattern. images taken at three spatial positions of the fringe pattern are processed in real time to produce optically sectioned images which are substantially similar to those obtained with confocal microscopes.

关键词： 3D microscopy confocal microscopy fluorescence microscopy

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Polarised light microscopy

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Torok, P Higdon, PD Wilson, T Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

ISBN: (纸本)0819427004

In this work we discuss the role of polarisation in confocal microscopy. The effect of the optical system on light polarisation is analysed in the absence of a specimen. We also present numerical results on confocal microscopes imaging small dielectric scatterers and suggest an optimum pinhole size for crossed linear polars. Finally, imaging of large spherical scatterers and contrast optimisation of the optical system is discussed.

关键词： confocal microscopy polarised light vectorial theory high aperture theory

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Three-dimensional reconstruction by focus sensing from conventional 2D image slices

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Nahm, KB Shin, ES Ryoo, SM Hallym Univ Dept Phys Chunchon 200702 Kangwon South Korea

ISBN: (纸本)0819427004

We introduce an optical means of reconstructing the topology of rough objects observed under the microscope or at a relatively high magnification. First, 2D images were obtained under the conventional optical microscope or under the macroscopic imaging system, focused on differing height of the sample. The depth information was extracted by sensing the focus of each pixel of the conventional image slice. Each sample height associated with the best "focus figure" for each pixel was marked and were later utilized to generate the three dimensional coordinate map.

关键词： surface reconstruction microscopy focus sensing image slice

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acquisition of multiple image stacks with a confocal laser scanning microscope

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Zuschratter, W Steffen, T Braun, K Herzog, A Michaelis, B Scheich, H Fed Inst Neurobiol D-39118 Magdeburg Germany

ISBN: (纸本)0819427004

image acquisition at high magnification is inevitably correlated with a limited view over the entire tissue section. To overcome this limitation we designed software for multiple image-stack acquisition (3D-MISA) in confocal laser scanning microscopy (CLSM). The system consists of a 4 channel Leica CLSM equipped with a high resolution z-scanning stage (Leica Lasertechnik) mounted on a xy-motorized stage (Marzhauser). The 3D-MISA software is implemented into the microscope scanning software (Scanware, Leica Lasertechnik) and uses the microscope settings (magnification, zoom) for the movements of the xy-stage. It allows storage and recall of 70 xyz-positions and the automatic 3D-scanning of image arrays between selected xyz-coordinates. The number of images within one array is limited only by the amount of disk space or memory available. Although for most applications the accuracy of the xy-scanning stage is sufficient for a precise alignment of tiled views, the software provides the possibility of an adjustable overlap between two image stacks by shifting the moving steps of the xy-scanning stage. After scanning a tiled image gallery of the extended focus-images of each channel will be displayed on a graphic monitor. In addition, a tiled image gallery of individual focal planes can be created. In summary, the 3D-MISA allows 3D-image acquisition of coherent regions in combination with high resolution of single images.

关键词： multi-image-stacks confocal laserscanning microscopy 3D image acquisition

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Transmission confocal microscopy: making it a reality

引用

conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Arnison, MR Fekete, PW Serrano, M Nguyen, PTA Romagnoli, R Guan, L O'Byrne, JW Cogswell, CJ Univ Sydney Sch Phys Dept Phys Opt Sydney NSW 2006 Australia

ISBN: (纸本)0819427004

Imaging thick specimens in 3D transmission confocal modes presents two key problems. The first problem is variable aberrations introduced by changes in refractive index. The second problem is revealed when visualising acquired data, where thick 3D datasets are difficult to interpret. In this paper we present our emerging solutions to these problems. Aberrations can be classified as simple tip-tilt deflection of the beam, or more complicated higher order aberrations. We discuss our results which demonstrate successful on-the-fly detection and correction for tip-tilt For detecting higher order aberrations, we have chosen to investigate the wavefront curvature sensing technique. The second problem of rendering thick 3D datasets can be solved by extracting features of interest from the background. Simple intensity threshholding is not sufficient for complex biological specimens, and image processing in only two dimensions neglects any three dimensional structure. Use of Kohonen's self-organising map neural network in 3D results in clear segmentation of features for sample chromosome specimens.

关键词： 3D microscopy confocal transmission DIC adaptive optics neural network image processing

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