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IEEE International conference on image processing

作者： A. Accardo G. Candido R. Toffanin F. Vittur V. Jellus DEEI DEEI University of Trieste Italy Dept. BBCM University of Trieste Italy IMS Slovak Academy of Sciences Bratislava Slovakia

Magnetic resonance imaging (MRI) has recently been applied to the study of osteoporosis. Accurate acquisition techniques and image analysis protocols for the determination of trabecular bone structure are yet to be defined. Two different approaches for three-dimensional acquisition and reconstruction are exploited to derive the structural parameters of trabecular bone explants. In particular, MR microscopy (/spl mu/MRI) is used to obtain conventional (FT) and projection reconstruction (PR) spin-echo images (41/spl times/41/spl times/41 /spl mu/m/sup 3//voxel). Segmentation of the high-resolution images into bone and bone marrow is obtained by using a suitable threshold. The main bone structural parameters are then derived by using a 3D version of the directed secant algorithm applied to a series of contiguous sections. The efficiency of the two methods in the assessment of trabecular bone architecture is discussed.

关键词： Cancellous bone Magnetic resonance imaging image analysis image resolution Osteoporosis image reconstruction microscopy image segmentation Histograms Protocols

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The HISCORE camera a real time three dimensional and color camera

The HISCORE camera a real time three dimensional and color c...

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IEEE International conference on image processing

作者： F. Forster P. Rummel M. Lang B. Radig CT MS3 Siemens AG Munich Germany Institute for Human-Machine Communication Technische Universität München Munich Germany Informatics IX Image Understanding and Knowledge-Based Systems Technische Universität München Munich Germany

A new real time 3D-data and color acquisition system for the mid-distance range (0.5-3 m) employing color-encoded structured light is presented. The system is integrated using low-cost components and allows the combination of 2D and 3D image processing algorithms since it provides a 2D-color image of the scene in addition to the range data. Its design is focused on enabling the system to operate reliably in real-world scenarios, i.e. in uncontrolled environments and with arbitrary scenes. To that end novel approaches for encoding and recognizing the projected light are used which make the system practically independent of intrinsic object colors and minimize the influence of the ambient light conditions. Experimental results obtained for the first applications of the system, 3D face and gesture recognition tasks, are presented.

关键词： Cameras Layout Real time systems Face recognition Computed tomography Man machine systems Knowledge based systems image processing image coding Data acquisition

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Quantitative Characterization of Deformation and Damage in Shale Under Compression Utilizing Digital image Correlation

Quantitative Characterization of Deformation and Damage in S...

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2022 SPE/AAPG/SEG Unconventional Resources Technology conference, URTC 2022

作者： Nath, Fatick Aguirre, Edgardo Aguirre, Gabriel Perez, Jose L. Netro, Cesar Borjas, Flavio Limon, Valeria School of Engineering Texas A&M International University LaredoTX United States

Shale formations are constantly facing challenges ensuring safe drilling and effective fracturing to unlock potential. One of the key influencing factors is accurately characterizing the deformation and damage due to its intrinsic lamination and natural fractures. Moreover, shale formations exhibit fine-scale heterogeneity while the conventional laboratory methods (LVDT, strain gauges, etc.) are unable to distinguish such variation. Besides, methods like acoustic emission (AE), X-ray, computed tomography (CT), and scanning electron microscopy (SEM) are lengthy, costly, and functional with only small test ranges. This study aims to investigate the damage and deformation characteristics in shale samples using three-dimensional digital image correlation (3D-DIC) to overcome such limitations. A set of samples from Wolfcamp, Mancos, and Eagle Ford shale are tested under uniaxial (D×L=2"×2") and diametrical (D×L=2"×1") compression using a precision 100kN Instron electromechanical load frame with a constant displacement of 0.05 mm/min. Specimens had distinct lamination and natural fractures. An ARAMIS 3D-DIC image acquisition system was positioned to monitor the samples at a rate of 2 frames per second in a non-contact manner while conducting the uniaxial and diametrical compression experiment. A black-in-white dot pattern is utilized on the specimen to track the deformation while loading. The ARAMIS 3D-DIC system is used for image processing, visualization, and analysis of the shale damage process with varying load conditions. DIC generated quantitative full-field strain maps with load (tension, compression, and shear) show all sequences in the damage process and strain evolution. The entire damage progress over time of shale samples is split into four stages: initial damage stage, linear elastic, elastic-plastic, and plastic damage stage, which are evaluated by damage variables. In uniaxial and diametrical compression, Eagle Ford shale exhibits central failure due to ten

关键词： Computerized tomography

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On-line 3-dimensional confocal imaging in vivo

On-line 3-dimensional confocal imaging in vivo

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Annual International conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

作者： J. Li J.V. Jester H.D. Cavanagh W.M. Petroll University of Texas Southwestern Medical Centre Dallas TX USA Department of Opthamology University of Texas Southwestern Medical Centre Dallas TX USA

Tandem Scanning Confocal microscopy (TSCM) is ideal for in vivo corneal imaging, but currently requires time-consuming off-line image processing. To overcome this limitation, we have developed a novel PC-based TSCM system. The system is the first to provide interactive 3-D online image acquisition and analysis of in vivo confocal images.

关键词： In vivo Optical imaging Biomedical imaging Pixel Cornea microscopy Optical feedback Lenses Biomedical optical imaging image processing

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Multi-dimensional microscopy and its application in investigating instant effects of radiations on living cells

Multi-dimensional microscopy and its application in investig...

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The 3rd Asian and Oceanian conference on Photobiology

作者： Yao-Xiong Huang Institute of Biomedical Engineering Ji Nan University Guang Zhou China.

＜正＞The present paper introduces our newly developed techniques of multi-dimensional microscopy for measuring the structure and functions of living cells, simultaneously at cellular, sub-cellular, and intracellular molecule levels. The technical system is based on an inverted fluorescent microscope, its information acquisition and processing is organized in 8-dimensions: 3 dimensional image is acquired by a 3 dimensional relief image construction system;the 4th dimension （wave length） is taken by a fast micro-spectrophotometry;which, accompanied with a dynamic image analyzing system, also gives the information in the 5th dimension （time） by giving the absorption spectra and images in a time-lapse mode;the 6th -8th dimensions are organized by the lights from the sample: the transmitted light, gives the information of image and absorption spectroscopy;the scattered light, gives the information about the dynamic parameters of intracellular molecule and the cell membrane, as well as the chemical structure and concentration of the molecules;the fluorescence, gives the information of the labeled molecules in the cell. Therefore, the multi-dimensional microscopy is capable of performing measurement on the parameters of single intact cells include: 1) the chemical structure and concentration as well as the hydrodynamic radius of the intracellular molecules;2) the 3-D morphology and molecular distribution of the cell;3) the functional change of the intracellular protein;4) the flexibility and rigidity of the cell membrane;5) the differentiation, proliferation and the activity of the cell.

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Enhancing the Visualization of the Microvasculature of Extrahepatic Bile Ducts Obtained from Confocal microscopy images

Enhancing the Visualization of the Microvasculature of Extra...

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Brazilian Symposium on Computer Graphics and image processing (SIBGRAPI)

作者： Lizeth A. C. Beltran Jorge Luiz dos Santos Carolina Uribe Cruzz Carla M. D. S. Freitas Institute of Informatics Federal University of Rio Grande do Sul UFRGS Porto Alegre Brazil Universidade da Beira Interior Covilha Castelo Branco PT Lab. Exp. de Hepatologia e Gastroenterologia Porto Alegre Brazil

ISBN: (纸本)9781509035694;9781509035687

Confocal microscopy is an important tool for visualizing 3D datasets of fluorescent specimens, and has been used to investigate the structure of biological specimens. However, such images are affected by the noise introduced during the specimen preparation and image acquisition processes. Anisotropic diffusion is a non-linear filter that can significantly improve image quality while removing noise without blurring edges. This study investigates the application of anisotropic diffusion in confocal microscopy images by exploring different models for parameters' calculation. Our data consists of several slices of extrahepatic bile ducts containing a network of small vessels named Peribiliary Vascular Plexus (PVP), which are affected by several sources of noise. Experimental results show that anisotropic diffusion improved the volumetric visualization of the PVP. We validated the results using MSE and PSNR quantitative approaches and qualitative description by an expert user.

关键词： microscopy Ducts Anisotropic magnetoresistance Mathematical model Estimation Mice Three-dimensional displays

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A study on image processing Method for Noise Reduction in Digital Holographic microscopy

A study on Image Processing Method for Noise Reduction in Di...

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International Symposium on Environmentally Conscious Design and Inverse Manufacturing (EcoDesign)

作者： Masaharu Nagashima Hyun-Woo Kim Myungjin Cho Min-Chul Lee Graduate School of Computer Science and Systems Engineering Kyushu Institute of Technology Iizuka city Japan School of ICT Robotics and Mechanical Engineering IITC Hankyong National University Anseong-si Korea

ISBN: (数字)9798350363043

ISBN: (纸本)9798350363050

Digital Holographic microscopy (DHM) can obtain three-dimensional (3D) information about the fine structure of an object by utilizing the phase information of coherent light. In DHM, during the process of reconstructing the shape of an object, phase information is extracted by windowing the sideband containing the phase information. The 3D information of the object is then obtained from the acquired phase information. When windowing the sideband in a narrow range, it can remove noise caused by the DC spectrum, but detailed shape information of the object is lost. On the other hand, when windowing in a wide range, detailed shape information of the object can be obtained, but noise from the DC spectrum increases. To solve this trade-off relationship, we propose a method of determining the range for windowing the sideband using a function and then averaging the windowed sidebands. This proposed method can reduce the impact of noise from the DC spectrum and enables the acquisition of the detailed shape information. In this paper, we explain the reconstruction algorithm of this method and compare it with conventional methods and the previous study, phase differences averaging (PDA).

关键词： Three-dimensional displays Filtering Shape microscopy Noise Noise reduction Reconstruction algorithms Amplitude modulation Data mining Informatics

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Mechanical vibration compensation method for 3D+t multi-particle tracking in microscopic volumes

Mechanical vibration compensation method for 3D+t multi-part...

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Annual International conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

作者： A. Pimentel G. Corkidi Engineering and Computer Science Universidad Nacional Auténoma de México Mexico Laboratorio de Imágenes y Visión por Computadora Instituto de Biotecnología Universidad Nacional Auténoma de México Cuernavaca Mexico

The acquisition and analysis of data in microscopic systems with spatiotemporal evolution is a very relevant topic. In this work, we describe a method to optimize an experimental setup for acquiring and processing spatiotemporal (3D+t) data in microscopic systems. The method is applied to a three-dimensional multi-tracking and analysis system of free-swimming sperm trajectories previously developed. The experimental set uses a piezoelectric device making oscillate a large focal-distance objective mounted on an inverted microscope (over its optical axis) to acquire stacks of images at a high frame rate over a depth on the order of 250 microns. A problem arise when the piezoelectric device oscillates, in such a way that a vibration is transmitted to the whole microscope, inducing undesirable 3D vibrations to the whole set. For this reason, as a first step, the biological preparation was isolated from the body of the microscope to avoid modifying the free swimming pattern of the microorganism due to the transmission of these vibrations. Nevertheless, as the image capturing device is mechanically attached to the ldquovibratingrdquo microscope, the resulting acquired data are contaminated with an undesirable 3D movement that biases the original trajectory of these high speed moving cells. The proposed optimization method determines the functional form of these 3D oscillations to neutralize them from the original acquired data set. Given the spatial scale of the system, the added correction increases significantly the data accuracy. The optimized system may be very useful in a wide variety of 3D+t applications using moving optical devices.

关键词： Vibrations Spatiotemporal phenomena Optimization methods Piezoelectric devices Optical microscopy Optical devices Data analysis Evolution (biology) Biomedical optical imaging High speed optical techniques

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Optimised reflection imaging for surface roughness analysis using confocal laser scanning microscopy and height encoded image processing

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Journal of Physics: conference Series 2005年第1期13卷

作者： S J Tomovich Z Peng School of Engineering James Cook University Townsville Qld 4811 Australia

Quantitative surface measurement is an important field in engineering. Due to the complexity of surface topography, accurate surface characterisation requires three-dimensional (3D) surface measurements not provided by many current measurement systems. A nondestructive and versatile technique for quantifying 3D surface features is Confocal Laser Scanning microscopy (CLSM). However, there is little documentation on standard CLSM hardware settings required to capture images of suitable quality for 3D surface measurements. Understanding the complex relationship between CLSM settings, specimen properties and image quality is crucial to optimising the acquisition process for quantitative 3D surface measurements. The response of image quality to variations in CLSM hardware settings and specimen properties has been investigated in the study. Through the investigations, criteria have been developed to select optimal CLSM hardware settings to minimise image noise, eliminate image distortion, maximise contrast and resolution for reliable and accurate 3D numerical surface measurements. A reliable 3D image analysis system has been developed for image processing and surface measurement of engineering surfaces and small particles. The image analysis system developed in Matlab for the confocal system provides a new means to quantitatively characterise a wide range of engineering surfaces with accuracy and efficiency.

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