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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 559 条记录，以下是61-70 订阅

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Deformable mirror based remote focusing for fast three-dimensional microscopy 13

Deformable mirror based remote focusing for fast three-dimen...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Zurauskas, Mantas Frade, Maria Booth, Martin Univ Oxford Ctr Neural Circuits & Behav Mansfield Rd Oxford OX1 3SR England Univ Oxford Dept Engn Sci Parks Rd Oxford OX1 3PJ England

ISBN: (纸本)9781628419474

We present a deformable mirror based remote focusing method for three-dimensional imaging in high-resolution microscopy systems. The method relies on predefined mirror mode arrays that are obtained during initial mirror training step with a low complexity wavefront-sensing module. The imaging plane can be refocused over distances over a hundred times greater than the original depth of field of the objective lens along the optical axis at millisecond rates. We will demonstrate the combination of the remote focusing method with spatiotemporally focused two-photon excitation applied to three-dimensional imaging of biological samples.

关键词： Remote focusing adaptive optics three dimensional microscopy two photon microscopy

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Efficient calibration algorithm and calibration pattern for correcting distortions for three-dimensional image acquisition systems for microscopic applications

Efficient calibration algorithm and calibration pattern for ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Walecki, Wojtek J. Szondy, Fanny Sunrise Opt LLC Sunrise FL 33351 USA

ISBN: (纸本)9780819470362

We present novel method for fully automated detection of calibration features. It reduces computation effort when compared to traditional corner, edge, and conic detection algorithms. Our method employs generalized method based on properties of prospective projection invariants [1], but is also applicable for the optical systems suffering high order, non-spherical distortions. We also show an example when in presence of significant barrel distortion procedure developed for ideal pinhole camera system outlined in [1] does not converge to Unique solution. We suggest new modified algorithm which produces unique solution.

关键词： machine vision camera calibration image processing

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Tracking fluorescent spots in biological video microscopy

Tracking fluorescent spots in biological video microscopy

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Genovesio, A Olivo-Marin, JC Inst Pasteur Quantitat Image Anal Grp F-75724 Paris France

ISBN: (纸本)0819447641

The analysis of cell and pathogen movement and motility is a major topic in cell biology for which computerized methods are most needed. This study proposes a method to detect and track multiple moving biological objects in image sequences acquired through fluorescence video microscopy. The method enables the analysis of video microscopy image sequences in order to obtain reliable quantitative data such as number, position, speed and movement phases. The method consists of three stages. A stage of detection is performed through a multi-scale analysis of images using an undecimated wavelet transform. The next stage is the prediction of the state of each detected spot in the next frame using a Kalman filter and an adapted model. Then comes a stage of data association which constructs the tracks and refines the filters. Once all moving objects have been assigned with unique spatio-temporal paths, trajectories are analyzed in terms of different parameters relevant to the motility analysis of biological objects.

关键词： image processing tracking wavelets Kalman filter data associations biological video microscopy fluorescent spots

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Using out-of-focus light to improve image acquisition time in confocal microscopy

Using out-of-focus light to improve image acquisition time i...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Davis, BJ Karl, WC Goldberg, BB Swan, AK Ünlü, MS Boston Univ Dept Elect & Comp Engn Boston MA 02215 USA

ISBN: (纸本)0819456756

Light that would typically be discarded at a confocal microscope's detector pinhole will be collected and processed to allow a reduced spatial sampling rate and thus an improved image acquisition time. It is shown that collecting and appropriately processing the out-of-focus light will allow an axial sampling rate below that specified by the Nyquist criterion. To achieve this, a central detector pinhole and a number of out-of-focus regions are collected concurrently. This corresponds to imaging through several different channels, with differing point spread functions, in parallel. Since the spatial sampling rate is below the Nyquist frequency, aliasing occurs in the data from each of the channels. However, since the point spread functions are different, the aliasing effects are different in each channel. This allows the ensemble of aliased images to be processed into a sin-le dealiased and deconvolved image. This potential utility of out-of-focus light is demonstrated through simulated examples for differing collection schemes and scanning rates. Results are shown for under-sampling by up to a factor of four. Collecting the out-of-focus light also improves instrument collection efficiency.

关键词： confocal microscopy image acquisition time sampling rate aliasing out-of-focus light confocal pinhole high speed microscopy confocal collection efficiency

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Near-field optical and atomic force constraints for super-resolution 3D deconvolution in far-field optical microscopy

Near-field optical and atomic force constraints for super-re...

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conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Axelrod, N Radko, A Ben-Yosef, N Khatchatouriants, A Treinin, M Lewis, A Hebrew Univ Jerusalem Dept Appl Phys IL-91904 Jerusalem Israel

ISBN: (纸本)081943535X

We demonstrate that near-field optical and atomic force microscopy data can be used for super-resolution three-dimensional (3-D) image restoration in optical sectioning fluorescence microscopy. A crucial feature in this approach is the full integration of such data sets with digital far-field images. The scanned probe data is used to provide modalities for boundary constraints which define surface optical information and spatial domains of optical alterations in a sample with a spatial precision that has been unachievable in the past. A restoration algorithm that can use such a complex of data for 3-D image deconvolution is presented. It uses a Tikhonov-Miller regularization scheme and allows for the imposition of different types of constraints to obtain super-resolution deconvolved images. Performance was tested by using simulated 3-D imaging. An example is given of the restoration of a 3-D wide field optical image of a biological specimen in the presence of atomic force constraints.

关键词： image restoration deconvolution algorithm constraints probe microscopy NSOM super-resolution

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Quasi-confocal fluorescence sectioning with dynamic speckle illumination

Quasi-confocal fluorescence sectioning with dynamic speckle ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Ventalon, Cathie Mertz, Jerome Boston Univ Dept Biomed Engn 44 Cummington St Boston MA 02215 USA

ISBN: (纸本)0819461326

We present a new fluorescence microscopy technique that provides depth discrimination in thick tissue. The technique relies on a simple modification to a conventional wide-field microscope, and consists in illuminating the sample with a sequence of speckle patterns and displaying the RMS or the variance of the resultant sequence of fluorescent images. We demonstrate quasi-confocal optical sectioning with an axial resolution of 5 microns FWHM. The lateral resolution is identical to the widefield microscope, namely 0.6 micron FWHM. images of a mouse brain are compared with standard wide-field images, demonstrating an efficient quasi-sectioning capacity in thick tissue throughout approximatively 100 microns depth. This is achieved because of the high contrast maintained by speckle in a scattering media.

关键词： fluorescence microscopy optical sectioning 3D imaging linear microscopy confocal microscopy speckle structured light illumination thick tissue imaging

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Fast scanning confocal microscopy with a rotary mirror array

Fast scanning confocal microscopy with a rotary mirror array

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Chen, NG Zhu, Q Univ Connecticut Dept Elect & Comp Engn Storrs CT 06269 USA

ISBN: (纸本)0819447641

We have developed a novel confocal microscopy system, which can perform both axial and lateral scanning at an ultra high speed. A rotary mirror array (RMA) consists of 36 small mirrors deployed on the surface of a round plate with a small tilting angle and a discrete rotational symmetry. When the RMA rotates at a constant speed, the focal point is scanned inside a sample periodically and linearly. We have demonstrated a scanning rate of 2400 lines per second, which can be readily improved to more than 20000 lines per second. Our scheme can also be used in a fast scanning multi-photon microscope.

关键词： rotary mirror array fast scanning confocal microscopy optical coherence microscopy

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Generation and imaging with the third harmonic

Generation and imaging with the third harmonic

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conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Brakenhoff, GJ Squier, J Univ Amsterdam BioCentrum Dept Mol Cyt NL-1098 SM Amsterdam Netherlands

ISBN: (纸本)081943535X

In this paper we study the spatial distribution and wavefront characteristics of third harmonic generation in relation to some material and interface conditions over the focal region of the fundamental beam. We investigate, mostly from an experimental point of view, the implications the physics of the THG generation process has in situations where THG may be employed for -3D- imaging. Due to the non-linear character of the THG generation process it is inherently suitable for this application. For the first time images of the distribution of the THG radiation, as the interface is moved through focus, are shown. Experiments on closely spaced interfaces or bilayers confirm unambiguously the correctness of the vector model for THG generation (Ward et al. 1969) in uniform media. In view of these and other data the image formation, especially for biological objects, with THG radiation will be discussed.

关键词： THG third harmonic microscopy imaging

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A study for accelerating the speed of all-in-focus image processing 27

A study for accelerating the speed of all-in-focus image pro...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Wang, Lihui Tabata, Satoshi Takeuchi, Hirotoshi Ishikawa, Masatoshi Guangdong Acad Sci Guangdong Inst Semicond Ind Technol 363 Changxing Rd Guangzhou 510650 Peoples R China Univ Tokyo Grad Sch Informat Sci & Technol Bunkyo Ku 7-3-1 Hongo Tokyo 1138656 Japan

ISBN: (纸本)9781510632547

To capture an all-in-focus and 3D depth image, shape from focus method is widely used. The phase accuracy of the image candidates should be processed and adjusted beforehand Phase only correction method was employed in this work and to accelerate the processing speed, the image processing by Fast Fourier Transform (FFT) was optimized Meanwhile, the processing task was assigned in several parallel threads so that the performance would be improved. The method was used on a variable focus imaging system, and, as a result, the processing speed was improved to around 2.5-fps.

关键词： all-in-focus shape from focus phase-only correction method 3D imaging variable focus system

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High-resolution volumetric imaging using hybrid image of Fourier light-field microscopy and wide-field microscopy 29

High-resolution volumetric imaging using hybrid image of Fou...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXix

作者： Liu, Wenhao Jia, Shu Georgia Inst Technol Wallace H Coulter Dept Biomed Engn Atlanta GA 30332 USA Emory Univ Atlanta GA 30332 USA

ISBN: (纸本)9781510648043;9781510648036

We have developed wFLFM to further enhance the resolution by taking the advantage of both wide-field image and FLFM. We proposed the principle of wFLFM and verified the approach both numerically using simulated rings, and experimentally using phantom samples on the wFLFM system we constructed as a prototype. The principle of wFLFM requires only additional wild-field image at the aperture plane, making it readily compatible with various extended depth of focus wide-field modules, and the validating results showed a 2-3x enhancement in lateral resolution without cost in axial information, thereby advancing wFLFM as a promising high-resolution 3D imaging method.

关键词： Light-field microscopy Fourier light-field microscopy high resolution volumetric imaging

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