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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 559 条记录，以下是81-90 订阅

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Investigation of 3D microscopy using intensity diffraction tomography

Investigation of 3D microscopy using intensity diffraction t...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Anastasio, Mark A. Huang, Yin Gbur, Greg Carney, P. Scott IIT Dept Biomed Engn Chicago IL 60616 USA Univ North Carolina Charlotte Dept Phys & Opt Sci Charlotte NC USA Univ Illinois Dept Elect & Comp Engn Urbana IL USA

ISBN: (纸本)0819461326

Diffraction tomography (DT) is an established imaging technique for reconstructing the complex-valued refractive index distribution of a weakly scattering 3D sample. Due to experimental difficulties associated with the direct measurement of the phase of an optical wavefield, the effectiveness of DT for optical imaging applications has been limited. A theory of intensity diffraction tomography (I-DT) has been proposed to circumvent this phase retrieval problem. In this work, we review the features of I-DT reconstruction theory that are relevant to optical microscopy. Computer-simulation studies are conducted to investigate the performance of reconstruction algorithms for a proposed I-DT microscope. The effects of data noise are assessed, and statistically optimal reconstruction strategies that employ multiple detector planes are proposed.

关键词： diffraction tomography three-dimensional microscopy tomographic image reconstruction

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Single image sectioning using spatial frequency domain imaging 24

Single image sectioning using spatial frequency domain imagi...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIV

作者： Hoffman, Zachary R. DiMarzio, Charles A. Northeastern Univ 360 Huntington Ave Boston MA 02115 USA Draper Lab 555 Technol Sq Cambridge MA 02139 USA

ISBN: (数字)9781510605824

ISBN: (纸本)9781510605817;9781510605824

Structured illumination microscopy (SIM) works well to produce depth information by removing undesired light from out of focus planes within a specimen. However, it generally requires multiple images in order to produce sectioning. We show that, under the right conditions, we can leverage Spatial Frequency Domain Imaging (SFDI) to produce both sectioning and relative depth information about a specimen using only a single image. After processing, we find that the axial resolution is comparable to that of traditional 3 phase SIM. With this technique, we are able to produce real-time videos in-vivo at 21 frames per second. Additionally, no moving parts are needed to produce sectioning at depth.

关键词： Structured Illumination optical sectioning super-resolution incoherent SFDI

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Three dimensional imaging using phase retrieval with two focus planes 13

Three dimensional imaging using phase retrieval with two foc...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Ilovitsh, Tali Ilovitsh, Asaf Weiss, Aryeh Meir, Rinat Zalevsky, Zeev Bar Ilan Univ Fac Engn IL-5290002 Ramat Gan Israel Bar Ilan Univ Bar Ilan Inst Nanotechnol & Adv Mat IL-5290002 Ramat Gan Israel

ISBN: (纸本)9781628419474

This work presents a technique for a full 3D imaging of biological samples tagged with gold-nanoparticles (GNPs) using only two images, rather than many images per volume as is currently needed for 3D optical sectioning microscopy. The proposed approach is based on the Gerchberg-Saxton (GS) phase retrieval algorithm. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. In addition, since the method requires the capturing of two images only, it can be suitable for 3D live cell imaging. The proposed concept is presented and validated both on simulated data as well as experimentally.

关键词： Three-dimensional microscopy Cellular imaging gold nanoparticle Phase retrieval

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High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

High speed slit-scanning confocal laser microscopy with an a...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Han, SM Im, KB Park, H Kim, D Kim, BM Yonsei Univ Dept Biomed Engn Kangwon Do 220710 South Korea

ISBN: (纸本)0819456756

Using an acousto-optic beam deflector and a line scan camera, we constructed a non-mechanical, slit-scanning confocal microscope. It generates two-dimensional 512x512 images with speed of 60 frames/sec, which can be easily expanded, to 100 frames/sec. The measured axial and lateral resolutions of the system are 3.3 mu m and < 1 mu m, respectively, with a 50X objective. This simple design can produce frames rates faster than other commercial products with comparable resolution, which may be useful for analysis of rapid interactions in various biological applications.

关键词： confocal microscope three-dimensional microscopy acousto-optic deflector line scan CCD

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Phase mask optimization for 3D parallax EDF microscopy

Phase mask optimization for 3D parallax EDF Microscopy

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Beckers, Ingeborg E. Gierlak, Michael Hoeppel, Robert Landskron, Juergen Beuth Univ Appl Sci Berlin Dept Appl Phys Med Engn D-13347 Berlin Germany

ISBN: (纸本)9780819498625

Extended depth-of-field (EDF) microscopy is a well-investigated and very simple method to obtain projection images with an extended depth of focus. Despite its advantages of being a real-time method applicable to any microscopic mode with high lateral resolution that can be simply realized by extending a commercial microscope, the lack of z-correlation is still a problem. In this work we present a combined technique of EDF and stereomicroscopy. By cross-correlation depth information is obtained. Finally, 3D images are reconstructed for best phase masks and simulation results are evaluated experimentally.

关键词： 3D imaging real time imaging extended depth of field microscopy EDF space light modulation SLM stereoscopy parallax EDFP

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Dynamic structured illumination microscopy: focused imaging and optical sectioning for moving objects

Dynamic structured illumination microscopy: focused imaging ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Krzewina, Leo G. Kim, Myung K. Univ S Florida Dept Phys Tampa FL 33620 USA

ISBN: (纸本)0819461326

Structured illumination microscopy (SIM) is a valuable tool for three-dimensional microscopy and has numerous applications in bioscience. Its success has been limited to static objects, though, as three sequential image acquisitions are required per final processed, focused image. To overcome this problem we have developed a multicolored grid which when used in tandem with a color camera is capable of performing SIM with just a single exposure. images and movies demonstrating optical sectioning of three-dimensional objects are presented, and results of applying color SIM for wide-field focused imaging are compared to those of SIM. From computer modeling and analytical calculations a theoretical estimate of the maximum observable object velocity in both the lateral and axial directions is available, implying that the new method will be capable of imaging a variety of live objects. Sample images of the technique applied to lens paper and a pigeon feather are included to show both advantages and disadvantages of CSIM.

关键词： three-dimensional microscopy microscopy optical sectioning structured illumination

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Automated 3D dendritic spine detection and analysis from two-photon microscopy

Automated 3D dendritic spine detection and analysis from two...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Koh, IYY Lindquist, WB SUNY Stony Brook Dept Appl Math & Stat Stony Brook NY 11794 USA

ISBN: (纸本)0819439398

The functional significance of dendritic spines and their plasticity to a wide spectrum of developmental and pathological conditions has led to extensive studies based on spine morphology. The advances in image acquisition techniques and the associated generation of large 3D data sets of optical micrographs have not been accompanied by comparable advances in data analysis techniques. We present an automated 3D spine detection and quantification procedure suitable for images obtained by laser scanning microscopy. The image is first processed by deconvolution and the dendritic phase consisting of the neuronal cytoplasm is extracted by segmentation. Spines are detected as geometrical protrusions relative to the dendritic backbone, ks very thin necks may not be imaged, some spine 'heads' may be detached from the dendrite and are detected as detached components. These detected heads are merged with spine 'bases' where appropriate. Morphological characterizations on spine length, volume, density and shape classifications are obtained. For time-lapse data, images are registered and individual spines are traced through the image sequence. Successful comparison results on spine lengths and densities with manual analysis are obtained. This method is highly automatic and allows detailed and objective quantification of the structure and dynamics of dendritic spines, which can be important predictors for the function of neural networks.

关键词： image processing dendrite spine two-photon medial axis laser scanning microscopy

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Variable-focus microlenses as a potential technology for endoscopy

Variable-focus microlenses as a potential technology for end...

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conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Smith, PJ McCabe, EM Taylor, CM Selviah, DR Day, SE Commander, LG Univ Dublin Trinity Coll Dept Phys Dublin 2 Ireland

ISBN: (纸本)081943535X

Using a new device which contains an array of microlenses whose focal lengths can be electrically varied, we have been able to control the input from one microlens to a single mode fibre using an applied voltage. For such a microlens array many closely-spaced focal spots can be generated in parallel, and electrically switched to address, potentially, an array of receiver fibres. We show how the particular switching characteristics of the device, whereby the lenses switch from diverging to converging, serves in turn to disperse light and to focus it into the fibre.

关键词： microlenses endoscopy direct view microscopy

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Tomographic imagery by interference microscopy

Tomographic imagery by interference microscopy

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Dubois, A Guiot, E Schwartz, W Moneron, G Grieve, K Boccara, C CNRS UPR A0005 Ecole Super Phys & Chim Ind Lab Opt Phys F-75231 Paris France

ISBN: (纸本)0819456756

We have developed a white-light interference microscope as an alternative technique to conventional optical coherence tomography (OCT). The experimental setup is based on a Linnik interferometer illuminated with a tungsten halogen lamp. En face tomographic images are obtained in real-time without scanning by computing the difference of two phase-opposed interferometric images recorded by a CCD camera. The short coherence length of the source yields an optical sectioning ability with 0.7 mu m resolution (in water). Transverse resolution of 0.9 mu m is achieved by using high numerical aperture microscope objectives. A shot-noise limited detection sensitivity of 90 dB can be reached with similar to 1 s acquisition time. High-resolution images of mouse and tadpole embryos are shown.

关键词： interference microscopy three-dimensional microscopy optical coherence tomography medical and biomedical imaging

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A wide-field microscopy technique using a linear image sensor for obtaining 3D images

A wide-field microscopy technique using a linear image senso...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Macedo, MP Barata, AJ Fernandes, AG Correia, CM Inst Engn Coimbra Dept Phys & Math P-3030 Coimbra Portugal

ISBN: (纸本)0819456756

Three-dimensional (3D) microscopy is a huge field that includes several microscopy techniques. Among these techniques the confocal microscopy is widely known and used due to its improved axial resolution or optical sectioning ability. However its high cost and image acquisition time as well as low signal-to-noise ratio are important drawbacks. Other techniques use wide-field structured illumination to get depth information from the sample. The detectors in commercial microscopes are normally of the point detector type (PMTs) in confocal and area sensors (CCDs) in widefield microscopy. Examples of microscopes that take advantage of lower cost and faster readout cycles of linear image sensors are very uncommon. The purpose of this work is to develop a low-cost microscopy technique for obtaining 3D images with lower acquisition time and higher signal-to-noise ratio (SNR) than in classical confocal microcopy. It uses a linear CMOS image sensor and specific reconstruction algorithms will be able to extract 3D information from the distribution of light intensity collected. The sensor readout circuitry has been developed and tests are currently running in a laboratory prototype in reflection mode with an epi-illuminated configuration of scanning-stage type. The light source is a commercial incandescent lamp with regulated intensity. For the development, test and optimization of the reconstruction algorithms the object is mounted on a 3-axis translation stage to scan the object in three perpendicular directions. Contrast and resolution results obtained using a resolution test slide and preliminary images of integrated circuit (IC) bonding are presented.

关键词： 3D microscopy confocal microscopy reconstruction algorithms optical sectioning linear sensor

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