An analysis is presented of the image formation in widefield fluorescence microscopy using standard light. The region of support of the resulting optical transfer function is discussed.
ISBN:
(纸本)0819427004
An analysis is presented of the image formation in widefield fluorescence microscopy using standard light. The region of support of the resulting optical transfer function is discussed.
This paper describes a method for the improvement of biological images acquired using a Transmission Electronic Microscope (TEM). Several techniques are presented that deal with noise reduction, artifact removal and n...
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ISBN:
(纸本)0819427004
This paper describes a method for the improvement of biological images acquired using a Transmission Electronic Microscope (TEM). Several techniques are presented that deal with noise reduction, artifact removal and non-uniform illumination correction. Experimental results are shown.
We describe a method of obtaining optically sectioned fluorescence images in a widefield conventional microscope by interfering two beams on an object so as to illuminate it with a single spatial frequency fringe patt...
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ISBN:
(纸本)0819427004
We describe a method of obtaining optically sectioned fluorescence images in a widefield conventional microscope by interfering two beams on an object so as to illuminate it with a single spatial frequency fringe pattern. images taken at three spatial positions of the fringe pattern are processed in real time to produce optically sectioned images which are substantially similar to those obtained with confocal microscopes.
We introduce an optical means of reconstructing the topology of rough objects observed under the microscope or at a relatively high magnification. First, 2D images were obtained under the conventional optical microsco...
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ISBN:
(纸本)0819427004
We introduce an optical means of reconstructing the topology of rough objects observed under the microscope or at a relatively high magnification. First, 2D images were obtained under the conventional optical microscope or under the macroscopic imaging system, focused on differing height of the sample. The depth information was extracted by sensing the focus of each pixel of the conventional image slice. Each sample height associated with the best "focus figure" for each pixel was marked and were later utilized to generate the three dimensional coordinate map.
In this work we discuss the role of polarisation in confocal microscopy. The effect of the optical system on light polarisation is analysed in the absence of a specimen. We also present numerical results on confocal m...
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ISBN:
(纸本)0819427004
In this work we discuss the role of polarisation in confocal microscopy. The effect of the optical system on light polarisation is analysed in the absence of a specimen. We also present numerical results on confocal microscopes imaging small dielectric scatterers and suggest an optimum pinhole size for crossed linear polars. Finally, imaging of large spherical scatterers and contrast optimisation of the optical system is discussed.
In time-lapse microscopyimage quality is limited by instrumental and specimen characteristics. Moreover, an enormous amount of data may be accumulated. This challenges not only the development of new tools for image ...
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ISBN:
(纸本)081943535X
In time-lapse microscopyimage quality is limited by instrumental and specimen characteristics. Moreover, an enormous amount of data may be accumulated. This challenges not only the development of new tools for imageprocessing, but typically forces methods to be executed in combination. To remedy essential problems, an imageprocessing pipeline is employed which features image restoration, image compression, visualization and analysis. To control the flow of information within this pipeline and to optimize the parameter settings like compression rates or number of iterations, the information content is evaluated on the basis of the ratio of signal and noise.
This paper discusses some aspects of standardization in digital microscopy. Included are imageacquisition, use of image and graphics formats and data protocols in multidimensions, particular image analysis procedures...
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ISBN:
(纸本)0819439398
This paper discusses some aspects of standardization in digital microscopy. Included are imageacquisition, use of image and graphics formats and data protocols in multidimensions, particular image analysis procedures and use of industry standards for software development.
When recording three-dimensional (3D) images by the method of optical sectioning microscopy, each optical section contains the in-focus information plus out-of-focus contributions that obscure the in-focus detail and ...
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ISBN:
(纸本)0819456756
When recording three-dimensional (3D) images by the method of optical sectioning microscopy, each optical section contains the in-focus information plus out-of-focus contributions that obscure the in-focus detail and reduce contrast. There are several methods to remove or prevent the out-of-focus contributions from the stack of optical sections. One such method is image estimation -the use of a computer program based on a mathematical description of the microscope to remove the out-of-focus contributions. Another method is the use of structured illumination and a simple arithmetic operation to obtain a image that in which the out-of-focus contributions are greatly reduced. We derived a method for image estimation that uses the images collected from the structured-illumination microscope. The method improves the resolution of small detail over that possible with the structured illumination using the simple arithmetic formula.
En-face optical coherence tomography (OCT) employing parallel heterodyne detection technique is demonstrated for three-dimensionalmicroscopy. To enable the use of commercially available CCD cameras as two-dimensional...
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ISBN:
(纸本)0819443603
En-face optical coherence tomography (OCT) employing parallel heterodyne detection technique is demonstrated for three-dimensionalmicroscopy. To enable the use of commercially available CCD cameras as two-dimensional heterodyne detector arrays in OCT imaging, a frequency synchronous detection method is Employed. Depth-resolved, full-field images are acquired at 30 Hz video-rate without lateral scanning.
A new time-domain two-dimensional fluorescence lifetime detection method and a fluorescence lifetime imaging microscope that is based on a synchroscan streak camera are presented. This system can generate in parallel ...
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ISBN:
(纸本)0819452327
A new time-domain two-dimensional fluorescence lifetime detection method and a fluorescence lifetime imaging microscope that is based on a synchroscan streak camera are presented. This system can generate in parallel a complete two-dimensional fluorescence lifetime image without scanning the light. We demonstrate the acquisition of a fluorescence lifetime image of a semiconductor ET material sample at a temporal resolution of 2ps and spatial resolution of 4mum within 40ms.
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