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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing V"

共 708 条记录，以下是21-30 订阅

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Multi-immersion open-top light-sheet microscopy 27

Multi-immersion open-top light-sheet microscopy

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXvII

作者： Glaser, Adam K. Liu, Jonathan T. C. Univ Washington Dept Mech Engn Seattle WA 98195 USA

We present an easy-to-use multi-immersion open-top light-sheet microscope designed specifically for high-throughput imaging of a diverse set of tissues prepared with a variety of clearing protocols.

ISBN: (纸本)9781510632547

We present an easy-to-use multi-immersion open-top light-sheet microscope designed specifically for high-throughput imaging of a diverse set of tissues prepared with a variety of clearing protocols.

关键词： light-sheet microscopy selective-plane illumination microscopy fluorescence microscopy

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A study for accelerating the speed of all-in-focus image processing 27

A study for accelerating the speed of all-in-focus image pro...

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXvII

作者： Wang, Lihui Tabata, Satoshi Takeuchi, Hirotoshi Ishikawa, Masatoshi Guangdong Acad Sci Guangdong Inst Semicond Ind Technol 363 Changxing Rd Guangzhou 510650 Peoples R China Univ Tokyo Grad Sch Informat Sci & Technol Bunkyo Ku 7-3-1 Hongo Tokyo 1138656 Japan

ISBN: (纸本)9781510632547

To capture an all-in-focus and 3D depth image, shape from focus method is widely used. The phase accuracy of the image candidates should be processed and adjusted beforehand Phase only correction method was employed in this work and to accelerate the processing speed, the image processing by Fast Fourier Transform (FFT) was optimized Meanwhile, the processing task was assigned in several parallel threads so that the performance would be improved. The method was used on a variable focus imaging system, and, as a result, the processing speed was improved to around 2.5-fps.

关键词： all-in-focus shape from focus phase-only correction method 3D imaging variable focus system

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Auto-correlation for multi-view deconvolved reconstruction in light sheet microscopy 28

Auto-correlation for multi-view deconvolved reconstruction i...

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Three-dimensional and multidimensional microscopy: image acquisition and processing XXvIII 2021

作者： Ancora, Daniele valentini, Gianluca Pifferi, Antonio Giovanni Bassi, Andrea Dipartimento di Fisica Politecnico di Milano Piazza Leonardo da Vinci 32 Milano20133 Italy Istituto di Fotonica e Nanotecnologie Consiglio Nazionale delle ricerche Piazza Leonardo da Vinci 32 Milano20133 Italy

ISBN: (数字)9781510641341

ISBN: (纸本)9781510641334

Tomographic inspection of fluorescent labels distributed within a specimen is an important aspect in biology. Light sheet fluorescent microscopy (LSFM) offers a powerful and simple tool to selectively slice the sample and let us directly obtain a tomographic view of the specimen. However, due to non-isotropic resolution of the technique along the axial scanning, one may want to combine different views of the object and add deconvolution to the process in order to achieve higher resolution. Typically, multi-view Bayesian methods based on Richardson-Lucy deconvolution are used for this task once the datasets are exactly registered against each other. In this work, instead, we begin to investigate how to avoid the alignment procedure and use a direct algorithm to form a multi-view tomographic reconstruction. To do this, we developed a new framework based on auto-correlation analysis that let us achieve deconvolved reconstructions starting from blurred auto-correlations. Since the latter are insensitive to shifts, we can combine the auto-correlations coming from multi-view acquisitions without taking care of the registration procedure. © 2021 Copyright SPIE.

关键词： Inverse problems

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3,3′-thiodipropanol as a refractive index matching mounting medium for fluorescence microscopy 27

3,3′-thiodipropanol as a refractive index matching mounting...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXvII

作者： Alata Tejedo, Milvia Iris Martinez Cervantes, Juan Carlos Piazza, valeria Ctr Invest Opt AC Loma Bosque 115 Guanajuato Mexico

ISBN: (纸本)9781510632547

In this work, several tests of the performance of 3,3'-thiodipropanol (TDP) as a mounting medium for fluorescence microscopy of biological samples were performed. Besides optical properties like the dispersion curve of TDP and the effect of the embedding medium on the fluorescence of commonly used dyes, the interaction with biological specimens, including the labeling of filamentous actin with fluorescent phalloidins, was tested: TDP showed to represent an interesting alternative to commercial mounting media. By mixing TDP with 2,2'-thiodiethanol (TDE), it was possible not only to fine tune the refractive index of the resulting solution, but to preserve the compatibility with fluorescent phalloidins.

关键词： Mounting media fluorescence microscopy filamentous actin

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Computational super-resolution microscopy: leveraging noise model, regularization and sparsity to achieve highest resolution 27

Computational super-resolution microscopy: leveraging noise ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXvII

作者： Xing, Jian Chen, Simeng Becker, Stephen Yu, Jiun-Yann Cogswell, Carol Univ Colorado Dept Elect Comp & Energy Engn Boulder CO 80309 USA Univ Colorado Dept Appl Math Boulder CO 80309 USA

ISBN: (纸本)9781510632547

We report progress in algorithm development for a computation-based super-resolution microscopy technique. Building upon previous results, we examine our recently implemented microscope system and construct alternative processing algorithms. Based on numerical simulations results, we evaluate the performance of each algorithm and determine the one most suitable for our super-resolution microscope.

关键词： Super-resolution microscopy regularization computational microscopy

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Multifocus imaging via polarization wavefront shaping 27

Multifocus imaging via polarization wavefront shaping

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXvII

作者： Li, Chen Ding, Changqin Li, Minghe Rong, Jiayue Ulcickas, James Simpson, Garth J. Purdue Univ Dept Chem W Lafayette IN 47907 USA

ISBN: (纸本)9781510632547

Conventional three-dimensional (3D) images of biological samples are typically assembled from a stack of two-dimensional images acquired sequentially at different focal planes. This time-consuming manner hinders the application of 3D imaging techniques to the investigation of fast biochemical dynamics and light-sensitive biological events. The concept of multifocus imaging, which enables simultaneous acquisition of images from multiple focal planes, was introduced to achieve rapid 3D imaging. In the present study, we achieved multifocus imaging through polarization wavefront shaping via a micro-retarder array which splits the incident linearly polarized light into three beamlets that are focused to three axially-offset focal planes with 100 itm separation. Append to an existing beam-scanning microscope, this multifocus system enables rapid 3D imaging compatible with a variety of optical microscopic approaches including laser transmittance, two-photon excited fluorescence, and second harmonic generation microscopy.

关键词： Multifocus imaging beam scanning microscopy polarization wavefront shaping Jones calculus

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PHASE RETRIEvAL FROM 4-dimensional ELECTRON DIFFRACTION DATASETS

PHASE RETRIEVAL FROM 4-DIMENSIONAL ELECTRON DIFFRACTION DATA...

引用

IEEE International conference on image processing (ICIP)

作者： Friedrich, Thomas Yu, Chu-Ping verbeek, Johan Pennycook, Timothy van Aert, Sandra Univ Antwerp Electron Microscopy Mat Sci EMAT Groenenborgerlaan 171 B-2020 Antwerp Belgium Univ Antwerp NANOlab Ctr Excellence Groenenborgerlaan 171 B-2020 Antwerp Belgium

ISBN: (纸本)9781665441155

We present a computational imaging mode for large scale electron microscopy data, which retrieves a complex wave from noisy/sparse intensity recordings using a deep learning approach and subsequently reconstructs an image of the specimen from the Convolutional Neural Network (CNN) predicted exit waves. We demonstrate that an appropriate forward model in combination with open data frameworks can be used to generate large synthetic datasets for training. In combination with augmenting the data with Poisson noise corresponding to varying dose-values, we effectively eliminate overfitting issues. The U-NET[1] based architecture of the CNN is adapted to the task at hand and performs well while maintaining a relatively small size and fast performance. The validity of the approach is confirmed by comparing the reconstruction to well-established methods using simulated, as well as real electron microscopy data. The proposed method is shown to be effective particularly in the low dose range, evident by strong suppression of noise, good spatial resolution, and sensitivity to different atom types, enabling the simultaneous visualisation of light and heavy elements and making different atomic species distinguishable. Since the method acts on a very local scale and is comparatively fast it bears the potential to be used for near-real-time reconstruction during data acquisition.

关键词： phase retrieval inverse problem electron diffraction 4D-STEM CBED

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COMPUTATIONAL IMAGING IN 3D X-RAY microscopy: RECONSTRUCTION, image SEGMENTATION AND TIME-EvOLvED EXPERIMENTS

COMPUTATIONAL IMAGING IN 3D X-RAY MICROSCOPY: RECONSTRUCTION...

引用

IEEE International conference on image processing (ICIP)

作者： Niverty, Sridhar Torbatissaraf, Hamid Nikitin, v De Andrade, vincent Niauzorau, S. Kublik, N. Azeredo, B. Tekawade, A. De Carlo, Francesco Chawla, Nikhilesh Purdue Univ Sch Mat Engn W Lafayette IN 47907 USA Argonne Natl Lab Adv Photon Source Argonne IL 60439 USA Arizona State Univ Sch Engn Matter Transport & Energy Tempe AZ USA

ISBN: (纸本)9781665441155

Time-dependent X-ray microscopy (XRM) is an excellent technique to develop a fundamental mechanistic understanding of material behavior. Computational imaging plays a critical role in XRM, in a variety of ways. 2D projections are acquired and the resulting datasets are reconstructed using a filtered back projection algorithm. Several imaging artifacts are typically present, such as beam hardening, misalignment of the data, drift during time-evolved experiments (particularly at high temperatures and/or nanometer resolution scans), etc. Minimizing and removing these artifacts is, thus, very important. This is all the more important, because image segmentation is then done to quantify the statistics of the microstructure (often, as a function of time). The efficiency and accuracy of image segmentation is directly proportional to the quality of the initial reconstructed data. Thus, there is a need to develop efficient, robust algorithms that can handle large datasets obtained by 4D, time-dependent x-ray microscopy. In this paper, we will describe the challenges associated with computation imaging during x- ray microscopy. The use of Convolutional Neural Network (CNN) architectures based on a deep learning approach, as means of automating and handling x-ray microscopy data sets, in both lab-scale and synchrotron, will be discussed. The use of CNN techniques to robustly process ultra-large volumes of data in relatively small-time frames can exponentially accelerate tomographic data analysis, opening up novel avenues for performing 4D characterization experiments.

关键词： image segmentation Temperature distribution Three-dimensional displays microscopy Synchrotrons Tomography Convolutional neural networks

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Evaluation of a combined two-color phase plate forming three-dimensional dark holes in super resolution microscopy 27

Evaluation of a combined two-color phase plate forming three...

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXvII

作者： Nagai, Koumei Maruyama, Takashi Kodaira, Akira Kumagai, Hiroshi Bokor, Nandor Iketaki, Yoshinori NTT Adv Technol Corp 3-1 Morinosato Wakamiya Atsugi Kanagawa 2430124 Japan Kitasato Univ Sch Allied Hlth Sci Phys 1-15-1 Kitasato Sagamihara Kanagawa 2520373 Japan Budapest Univ Technol & Econ Dept Phys Budafoki Ut 8 H-1111 Budapest Hungary Olympus Corp 2-3 Kuboyama Cho Hachioji Tokyo 1928512 Japan

ISBN: (纸本)9781510632547

In this study, a combined two-color phase plate (CTPP) was designed for super-resolution microscopy based on up conversion fluorescence depletion (FD), fabricated, and evaluated. It is composed of two types of phase plates, a spiral phase plate and an annular phase plate. A two-color phase plate modulates the phase of the erase beam while maintaining the phase of the pump beam. SRM performed using the proposed CTPP is expected to enable super resolution in both the focal plane and in the optical axis direction. Despite its complex structure, a highly accurate CTPP was obtained by using the exposure and etching processes used in semiconductor manufacturing.

关键词： Super-resolution microscopy Phase modulation Fluorescence depletion Optical-axis resolution

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A multiplexed confocal FLIM microscope with 4-taps time-gated imager 27

A multiplexed confocal FLIM microscope with 4-taps time-gate...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXvII

作者： Richards, Morgan Shirakawa, Yuya Badr, Fares Kagawa, Keiichiro Kawahito, Shoji Fang, Qiyin McMaster Univ Dept Engn Phys 1280 Main St West Hamilton ON L8S 4L7 Canada Shizuoka Univ Res Inst Elect 3-5-1 Johoku Hamamatsu Shizuoka 4328011 Japan McMaster Univ Sch Biomed Engn 1280 Main St West Hamilton ON L8S 4L7 Canada

ISBN: (纸本)9781510632547

Combined with confocal imaging, Fluorescence lifetime imaging microscopy (FLIM) can achieve 3-dimensional optical sectional capability with sub-nanosecond lifetime information. As confocal FLIM acquires multi-dimensional data 4D (3D space + time), it is inherently slow. Recent developments in lock-in pixel imagers with time gated pixels show such detectors are capable of collecting as many as 8-time gates in a single pixel cycle. We present a multiplexed confocal FLIM microscope, equipped with a 4-taps time-gated lock-in pixel imager. The multiplexing setup allows the use of the sparse array with sub-nanosecond time-gating to achieve high throughput FLIM acquisition.

关键词： Multipoint Confocal Fluorescence FLIM Time-resolved CMOS image sensor lock-in pixel biomedical imaging

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