Computer-Generated Holography (CGH) algorithms identify Three-dimensional (3D) holograms by utilizing focal stacks as their target images. However, periodically slicing depth planes to generate a focal stack can lead ...
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ISBN:
(纸本)9781510686069;9781510686076
Computer-Generated Holography (CGH) algorithms identify Three-dimensional (3D) holograms by utilizing focal stacks as their target images. However, periodically slicing depth planes to generate a focal stack can lead to artifacts and reduced contrast in visually salient parts on a 3D scene. We introduce a content-adaptive targeting scheme that prioritizes contrast and optimizes slicing of the depth planes. This way, our new targeting scheme reduces the slicing artifacts in reconstructed 3D holographic images.
image compression in microscopy is a valuable technique, in particular if applied to multidimensional data. Information-preserving and competitive information-losing compression is applied to microscopical data and th...
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ISBN:
(纸本)0819430757;9780819430755
image compression in microscopy is a valuable technique, in particular if applied to multidimensional data. Information-preserving and competitive information-losing compression is applied to microscopical data and the resulting image quality is evaluated on a quantitative basis both in the spatial and frequency domain. Included are image data featuring different signal-to-noise ratios, but also voxel data for volume representations as well as graphical data for surface representations. The effect of compression on 3-D visualization and image management with data bases is included.
Confocal theta microscopy improves the resolution of confocal laser scanning microscopes by instrumentally solving the problem of the inferior axial resolution. A technical variation of this microscopy technique, the ...
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ISBN:
(纸本)0819430757;9780819430755
Confocal theta microscopy improves the resolution of confocal laser scanning microscopes by instrumentally solving the problem of the inferior axial resolution. A technical variation of this microscopy technique, the Single-Lens Theta microscopy (SLTM), is designed to be easily adapted to any common confocal laser scanning microscope. With SLTM, different kinds of other microscopical techniques are possible.
An affordable, robust and easy-to-use fluorescence lifetime imaging microscopy (FLIM) workstation that is completely automated and does not need any difficult calibration procedure is introduced. The workstation consi...
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ISBN:
(纸本)0819430757;9780819430755
An affordable, robust and easy-to-use fluorescence lifetime imaging microscopy (FLIM) workstation that is completely automated and does not need any difficult calibration procedure is introduced. The workstation consists of a standard fluorescence microscope, a modulated excitation light source, a camera, a modulation signal generator and acquisition/processing software. An evaluation of the system and its critical components is presented.
We present a novel light efficient technique for obtaining optically sectioned images in wide-field microscopy. The technique is a further development of correlation microscopy and is based on using complementary stru...
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ISBN:
(纸本)0819430757;9780819430755
We present a novel light efficient technique for obtaining optically sectioned images in wide-field microscopy. The technique is a further development of correlation microscopy and is based on using complementary structured light patterns to illuminate the specimen together with uncomplicated post-processing of the captured images.
It is shown that based on spectrally selective excitation of individual molecules in the focus of a high NA lens together with position sensitive imaging sub-resolution imaging of three-dimensional structures can be r...
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ISBN:
(纸本)0819430757;9780819430755
It is shown that based on spectrally selective excitation of individual molecules in the focus of a high NA lens together with position sensitive imaging sub-resolution imaging of three-dimensional structures can be realised. The feasibility of the idea is demonstrated with NA=0.55 optics on a model system of pentacene molecules in p-terphenal host matrix.
A new approach to imaging degradation and annealing in situ in a range of II-vi materials is presented. The approach uses confocal photoluminescence imaging in a confocal microscope. Using this imaging technique, it i...
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ISBN:
(纸本)0819430757;9780819430755
A new approach to imaging degradation and annealing in situ in a range of II-vi materials is presented. The approach uses confocal photoluminescence imaging in a confocal microscope. Using this imaging technique, it is observed that rapid degradation is caused by the presence of macroscopic defects.
It is shown that based on spectrally selective excitation of individual molecules in the focus of a high NA lens together with position sensitive imaging sub-resolution imaging of three-dimensional structures can be r...
详细信息
ISBN:
(纸本)0819430757
It is shown that based on spectrally selective excitation of individual molecules in the focus of a high NA lens together with position sensitive imaging sub-resolution imaging of three-dimensional structures can be realised. The feasibility of the idea is demonstrated with NA = 0.55 optics on a model system of pentacene molecules in p-terphenal host matrix.
The use of three-dimensional reconstruction methods to estimate the cell volume of astroglial cells in primary culture was studied. Two-dimensional microscopic images were collected by using an automated image-acquisi...
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ISBN:
(纸本)0819430757;9780819430755
The use of three-dimensional reconstruction methods to estimate the cell volume of astroglial cells in primary culture was studied. Two-dimensional microscopic images were collected by using an automated image-acquisition system. The images were reconstructed by the Linear Maximum a Posteriori method and the non-linear Maximum Likelihood Expectation Maximization (ML-EM) method. A fast variant of the ML-EM method was also developed. Results of this study show that the ML-EM reconstructed images are adequate for the determination of volume changes in cells or parts thereof.
When recording three-dimensional (3D) images by the method of optical sectioning microscopy, each optical section contains the in-focus information plus out-of-focus contributions that obscure the in-focus detail and ...
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ISBN:
(纸本)0819456756
When recording three-dimensional (3D) images by the method of optical sectioning microscopy, each optical section contains the in-focus information plus out-of-focus contributions that obscure the in-focus detail and reduce contrast. There are several methods to remove or prevent the out-of-focus contributions from the stack of optical sections. One such method is image estimation -the use of a computer program based on a mathematical description of the microscope to remove the out-of-focus contributions. Another method is the use of structured illumination and a simple arithmetic operation to obtain a image that in which the out-of-focus contributions are greatly reduced. We derived a method for image estimation that uses the images collected from the structured-illumination microscope. The method improves the resolution of small detail over that possible with the structured illumination using the simple arithmetic formula.
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