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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

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High-contrast 3D microscopic imaging of deep layers in a biological medium

High-contrast 3D microscopic imaging of deep layers in a bio...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Faridian, Ahmad Pedrini, Giancarlo Osten, Wolfgang Univ Stuttgart Inst Tech Opt SCoPE Pfaffenwaldring 9 D-70569 Stuttgart Germany

ISBN: (纸本)9780819498625

Multilayer imaging of biological specimens is a demanding field of research, but scattering is one of the major obstacles in imaging the internal layers of a specimen. Although in many studies the biological object is assumed to be a weak scatterer, this condition is hardly satisfied for sub-millimeter sized organisms. The scattering medium is inhomogeneously distributed inside the specimen. Therefore, the scattering which occurs in the upper layers of a given internal layer of interest is different from the lower layers. That results in a different amount of collectable information for a specific point in the layer from each view. An opposed view dark-field digital holographic microscope (DHM) has been implemented in this work to collect the information concurrently from both views and increase the image quality. Implementing a DHM system gives the possibility to perform digital refocusing process and obtain multilayer images from each side without depth scanning of the object. The results have been presented and discussed here for a Drosophila embryo.

关键词： Digital holographic microscopy Multilayer imaging Three-dimensional microscopy image enhancement image fusion

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Autostereoscopic visualization of 3D time-varying complex objects in volumetric image sequences

Autostereoscopic visualization of 3D time-varying complex ob...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Benassarou, A. Valette, G. Debons, D. Remion, Y. Lucas, L. CReSTIC SIC Rue CrayeresBP 1035 F-51687 Reims 2 France 3DTV Solut F-51100 Reims France

ISBN: (纸本)9780819484413

This paper presents 4dVizMed, a framework for interactive analysis and autostereoscopic visualization of 3d time-varying objects in volumetric image sequences. It combines a deformable surface model which automatically tracks volumetric features, real-time multi-view stereo volume rendering, and some interactive tools for manipulation and quantization. Our method is based on a topological feature tracking process, using a flow-based paradigm and a deformable surface model. It tracks through time the evolution of the components of an isosurface and their interaction with other components. We focus on the difficulties of visualizing 4d volume data, and we report the results of preliminary experiments designed to evaluate the utility of autostereoscopic displays for this purpose.

关键词： Visualization 3D modeling 3D image processing Autostereoscopic displays image visualization image segmentation 3D displays 3D visualizations image processing Volume rendering

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Application of phase retrieved pupil functions in wide-field fluorescence microscopy

Application of phase retrieved pupil functions in wide-field...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing IX

作者： Hanser, BM Gustafsson, MGL Agard, DA Sedat, JW Univ Calif San Francisco Grad Grp Biophys San Francisco CA 94143 USA

ISBN: (纸本)0819443603

multidimensional fluorescence microscopy data are often collected under imaging conditions that cause aberrations. For proper deconvolution of such data, one needs to generate a Point Spread Function (PSF) that includes the aberrations. Even if it is possible to model the imaging aberrations theoretically, there remains the problem of modifying the PSF accordingly. Measured PSFs are difficult to modify, and theoretical PSFs lack lens- and system-specific features. Phase retrieval approaches can be used to produce a compact and modifiable description of a microscope system, including its measured, system-specific performance. These pupil functions can be used in a depth-dependent deconvolution to correct for geometric distortions and improve restoration of relative intensities compared to spatially invariant deconvolution using unaberrated PSFs.

关键词： phase retrieval Point Spread Function (PSF) wide-field microscopy fluorescence microscopy Optical Transfer Function (OTF) phase diversity optical microscopy pupil function depth aberrations spherical aberrations

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Confocal microscopy with variable-focal length microlenses

Confocal microscopy with variable-focal length microlenses

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Mac Raighne, A Yang, LS Dunbar, LA McCabe, EM Scharf, T Trinity Coll Dublin Dept Phys Dublin 2 Ireland

ISBN: (纸本)0819452327

Confocal microscopy has a unique optical sectioning property, which allows three-dimensional images at different depths. Use of a microlens array is a potential alternative to the Nipkow disk for parallel imaging with high throughput in real-time confocal microscopy. The use of variable-focal-length microlenses can provide a way to axially scan the foci electronically avoiding the inflexible mechanical movement of the lens or the sample. Here we demonstrate a combination of a variable-focal-length microlens array and a fibre optic bundle as a way to create a high throughput aperture array that would be potentially applied as confocal imaging in vivo biological specimens. The incident side of the microlens array was determined by examining the focus distribution in the axial direction. The variation of the focal length obtained by changing the voltage and corresponding focus intensity were measured through a conventional microscope. Meanwhile, the fibre bundle was characterized by coupling with either coherent or incoherent light source. We use the fibre bundle as both a multiple aperture and an image-carrying element and combine it with a microlens array to built up a confocal system.

关键词： confocal microscopy endoscopy microlenses fibre bundle

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Pupil Polarimetry using Stress-Engineered Optical Elements

Pupil Polarimetry using Stress-Engineered Optical Elements

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Beckley, Amber M. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA

ISBN: (纸本)9780819479662

Stress-engineered optical elements show fascinating and potentially useful effects when placed at the pupil plane of an imaging system. When illuminated by a beam of spatially uniform polarization, a snapshot (single measurement) polarimetry method can be constructed. We expand upon this method to perform snapshot pupil polarimetry for spatially varying pupil polarization. We present the theory for snapshot non-uniform pupil polarization measurement using a stress-engineered optical element, as well as simulation results.

关键词： Polarimetry microscopy Cylindrical Vector Beams

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Microscopic tomography by digital interference holography

Microscopic tomography by digital interference holography

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Kim, MK Univ S Florida Dept Phys Tampa FL 33620 USA

ISBN: (纸本)0819452327

We are developing the digital interference holography (DIH) as a novel method of microscopic tomographic imaging by numerical superposition of a number of holographic fields taken with varying wavelengths. The digital interference holography does not involve pixel-by-pixel mechanical scanning of three-dimensional volume and yet achieves high lateral and longitudinal resolutions. The holographic interference pattern of an ob ect is generated optically and recorded digitally using a CCD camera. The hologrpahic image field is numerically calculated using basic diffraction formulas and the process is repeated for a range of varying wavelengths at regular intervals. Numerical superposition, or digital interference, of the holographic image fields yields the desired three-dimensional representation of the object. Experiments have demonstrated a few-micron lateral and axial resolutions. Furthermore, since the DIH is a coherent imaging system, one can form true tomographic images of sub-surface structures, in the presence of diffuse scattering from overlying layers. By being able to generate true tomographic images of subsurface structures, without the need for three-dimensional mechanical scannig, the DIH method can provide a very efficient and versatile imaging modality for a wide range of applications in materials science and biomedical imaging.

关键词： digital holography tomography microscopy multi-wavelength imaging

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Fluorescence microscopy with isotropic resolution using three objectives 13

Fluorescence microscopy with isotropic resolution using thre...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XxiiI

作者： Huelsnitz, Thomas Kner, Peter Univ Georgia Coll Engn Athens GA 30602 USA

ISBN: (纸本)9781628419474

Widefield and confocal fluorescence microscopy using a single objective suffer from poor resolution and a strong anisotropy between the lateral and axial resolution. Coherently combining the excitation and emission from two coaxial objectives improves the axial resolution up to sevenfold, but leaves the lateral resolution unchanged. Here we investigate the coherent combination of three objectives to create a point spread function (PSF) that is isotropic with higher resolution in the plane of the objectives. We develop a theoretical framework for simulating the performance of interferometric imaging with three objectives. Using three identical objectives with a large working distance and 0.9 numerical aperture (NA), the full-width half maximum of the confocal PSF is 135 nm compared to the lateral FWHM of 274 nm for imaging with a single objective at a wavelength of 515 nm.

关键词： microscopy fluorescence microscopy interferometric imaging PSF Engineering

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Improved contrast by modal illumination in scanning reflectance confocal microscopy

Improved contrast by modal illumination in scanning reflecta...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIX

作者： Glazowski, Christopher Zavislan, James Univ Rochester Inst Opt Rochester NY 14623 USA

ISBN: (纸本)9780819488701

Scanning reflectance confocal microscopy (SRCM) is a flexible technology that provides cellular resolution images of tissue morphology with tailored resolutions and fields of view. However, how accurately an object is represented, in other words its fidelity, is critical in medical imaging and is not represented simply by optical resolution. In this work we characterize the SRCMs fidelity of images derived within turbid media. We present theoretical and experimental results showing the improved fidelity when using modal illumination. We investigated the use of TEM10 illumination and a novel implementation of Nomarski differential-interference-contrast (DIC). Using a repeatable, stable turbid phantom the system fidelity was characterized.

关键词： microscopy turbid speckle confocal NRDIC pupil engineering phantom

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Gabor wavelet transform for dynamic analysis in digital holographic microscopy

Gabor wavelet transform for dynamic analysis in digital holo...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Zhong, Jingang Weng, Jiawen Hu, Cuiying Jinan Univ Inst Optoelect Engn Guangzhou 510632 Guangdong Peoples R China

ISBN: (纸本)9780819479662

Real time dynamic analysis of micro-object is one of the significant advantages of digital holographic microscopy, but the process of the spatial filtering limits the application of dynamic and automatic analysis in digital holographic microscopy. In this paper, a numerical reconstruction technique by means of Gabor wavelet transform (GWT) is presented for real time dynamic analysis. Appling the GWT to the hologram, the quantitative phase information of the micro-object is obtained automatically without the process of the spatial filtering. An experiment with a sequence of holograms of an animalcule is proposed for the dynamic and automatic analysis by employing the GWT method.

关键词： Digital holographic microscopy dynamic analysis wavelet transform full-field quantitative phase imaging

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Programmable array microsope employing two ferroelectric liquid crystal spatial light modulators

Programmable array microsope employing two ferroelectric liq...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Taylor, CM McCabe, EM Univ Dublin Trinity Coll Dept Phys Dublin 2 Ireland

ISBN: (纸本)0819439398

We present a programmable array microscope (PAM) which uses a pair of ferroelectric liquid crystal spatial light modulators (FLC SLMs). The system is similar to a confocal imaging system called a direct-view microscope (DVM), in which scanned aperture arrays are used to obtain real-time confocal images. The PAM, unlike the DVM, allows arrays to be scanned electronically rather than mechanically. In our system, one SLM is placed in the source plane of a conventional microscope system;the other is placed in the detector plane. Confocal aperture arrays are displayed and scanned synchronously on the SLMs and confocal imaging results. The resolution is improved when compared to a similar previously presented system which employed a single SLR I. We present axial resolution measurements for a variety of array dimensions and investigate the use of aperture correlation techniques to improve the light throughput in the devices.

关键词： programmable array microscopy ferroelectric liquid crystal spatial light modulator confocal microscope direct-view microscopy

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