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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

共 561 条记录，以下是131-140 订阅

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Adaptive compensation of aberrations in ultrafast 3D microscopy using a deformable mirror

Adaptive compensation of aberrations in ultrafast 3D microsc...

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conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Sherman, L Albert, O Schmidt, C Vdovin, G Mourou, G Norris, TB Univ Michigan Ctr Ultrafast Opt Sci Ann Arbor MI 48109 USA

ISBN: (纸本)081943535X

3D imaging using a multiphoton scanning confocal microscope is ultimately Limited by aberrations of the system. We describe a system to adaptively compensate the aberrations with a deformable mirror. We have increased the transverse scanning range of the microscope by three with compensation of off-axis aberrations. We have also significantly increased the longitudinal scanning depth with compensation of spherical aberrations from the penetration into the sample. Our correction is based on a genetic algorithm that uses second harmonic or two-photon fluorescence signal excited by femtosecond pulses from the sample as the enhancement parameter. This allows us to globally optimize the wavefront without a wavefront measurement. To improve the speed of the optimization we use Zernike polynomials as the basis for correction. Corrections can be stored in a database for look-up with future samples.

关键词： scanning confocal microscopy aberration correction

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A compact open-top light-sheet microscope for Optofluidic imaging 29

A compact open-top light-sheet microscope for Optofluidic im...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIX

作者： Son, Jeonghwan Mandracchia, Biagio Jia, Shu Georgia Inst Technol Wallace H Coulter Dept Biomed Engn Atlanta GA 30332 USA Emory Univ Atlanta GA 30332 USA

ISBN: (纸本)9781510648043;9781510648036

The fluorescence Optofluidic imaging have recently transformed conventional optical investigations of biological systems by exploiting the synergic combination of recent microscopy and integrated chips technologies. The implementation of light-sheet microscopy on Optofluidic imaging has further pushed the systemic capabilities towards fast and 3D-volumetric imaging. Here, we propose a new compact open-top light-sheet microscope to extend the study of 3D-fluorescence Optofluidic imaging. The proposed portable architecture of the system and customized reconstruction algorithms enable providing high throughput and volumetric reconstruction capability with an isometric single-cell resolution. The experimental results demonstrate the applicability of open-top light-sheet configuration to single-cell imaging in a flow. We anticipate the system to offer promising applications in clinical settings where the portability and 3D-volumetric analysis are required for fast diagnostics.

关键词： Light-sheet microscopy Open-top Light-sheet microscopy Portable microscopy Optofluidic Optofluidic imaging 3D-Volumetric imaging Volumetric imaging High-throughput imaging

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Effects of specimen morphology on adaptive confocal and multiphoton microscopy

Effects of specimen morphology on adaptive confocal and mult...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Booth, Martin J. Wilson, Tony Univ Oxford Dept Engn Sci Parks Rd Oxford OX1 3PJ England

ISBN: (纸本)9780819465566

microscopy of thick biological specimens is often detrimentally affected by specimen-induced aberrations. In the simplest case, these aberrations arise from a refractive index mismatch between the immersion and mounting media. In other situations, the aberrations arise from variations in refractive index within the specimen. These aberrations cause loss of signal and reduced resolution. Aberrations can be corrected using adaptive optics, where a deformable mirror introduces equal but opposite aberrations into the optical path. Aberration correction can be performed by reconfiguring the deformable mirror and using the fluorescence signal as feedback, effectively maximising the fluorescence intensity. However, the degree to which aberrations affect the intensity is related to the distribution of fluorescence in the specimen. Signals from point-like objects are affected more than equivalent signals from from planar or volume objects. We investigate this effect and discuss the implications for adaptive optical microscopy of biological specimens.

关键词： confocal microscopy multiphoton microscopy aberrations adaptive optics

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Three-dimensional imaging with a quadrature tomographic microscope

Three-dimensional imaging with a quadrature tomographic micr...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Stott, JJ Bennett, RE Warner, CM DiMarzio, CA Northeastern Univ Ctr Subsurface Sensing & Imaging Syst Boston MA 02115 USA

ISBN: (纸本)0819439398

The quadrature tomographic microscope combines conventional microscopy with interferometric quadrature detection techniques to yield a simultaneous measurement of both the amplitude and phase of the electric field. This is of tremendous utility in imaging nearly-transparent objects since changes in phase can be directly attributed to changes in the object's index of refraction while the small amplitude changes provide additional structural information. In this paper we will discuss the progress we have made towards reconstructing three dimensional images from the measured two-dimensional complex electric field. BS applying an artificial curvature to the measured field and back-propagating the Gaussian beams, we are able to digitally manipulate the imaging kernel and image different focal planes.

关键词： microscopy optical quadrature tomography quantitative biology

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Performance evaluation of point-spread function engineering to reduce the impact of depth-induced aberrations on extended depth-of-field microscopy

Performance evaluation of point-spread function engineering ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIX

作者： Yuan, Shuai Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA

ISBN: (纸本)9780819488701

In this study, we evaluated a point-spread function (PSF) engineered using wave front encoding (WFE) and a generalized cubic phase mask (GCPM) design selected to reduce the impact of depth-induced spherical aberration (SA) on extended depth-of-field (EDOF) microscopy with high NA lenses. Mean-square-error based metrics computed from three-dimensional (3D) depth-variant WFE-PSFs with increasing amounts of SA were used to quantify the engineered PSF's sensitivity to SA and to compare it to the sensitivity of the cubic-phase mask (CPM) PSF traditionally used for EDOF microscopy. The potential performance of the engineered PSF with resilience to SA was further evaluated with simulations in which EDOF images of a 3D object were obtained by processing WFE images with and without SA. A qualitative and quantitative comparison of the EDOF images with the true object show that the WFE-PSF engineering with the selected GCPM design provides better performance in reducing the impact of SA. In addition, the GCPM-based EDOF images do not suffer from the known lateral shift of object features located away from the plane of focus encountered in traditional CPM-based EDOF images.

关键词： Extended depth-of-field microscopy point-spread function engineering wavefront encoding spherical aberration

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Optimization of spinning disk confocal microscopy: Synchronization with the ultra-sensitive EMCCD

Optimization of spinning disk confocal microscopy: Synchroni...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Chong, FK Coates, CG Denvir, DJ McHale, N Thornbury, K Hollywood, M Andor Technol Ltd Belfast BT12 7AL Antrim North Ireland

ISBN: (纸本)0819452327

The advent of Electron Multiplying Charge Coupled Device (EMCCD) technology and it's ability to overcome previous hurdles in low-light fluorescence microscopy, such as phototoxicity to live cells, photobleaching of fluorophores and exposure time restrictions, has resulted in a significant resurgence of interest in use of confocal spinning disk techniques for live cell microscopy. Here provide an understanding of, and technical solutions to, the issues of synchronization that have previously marred the coupling of fast CCD camera technology to confocal spinning disk arrangements. We examine the challenges arising from both old and new models of the Nipkow spinning disk confocal unit and suggest solutions throughout based on a sound comprehension of both (a) relative scan/exposure times;(b) relative orientation of the coupled devices;(c) optimisation of EMCCD clocking parameters.

关键词： CCD EMCCD Nipkow confocal spinning disk confocal microscopy live cell microscopy electron multiplying CCD single photon sensitivity

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Star Test Polarimetry using Stress-Engineered Optical Elements

Star Test Polarimetry using Stress-Engineered Optical Elemen...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIX

作者： Ramkhalawon, Roshita Beckley, Amber M. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA

ISBN: (纸本)9780819488701

Stress-engineered optical elements have potential applications in snapshot polarimetry, in which a single irradiance image is used to measure a spatially varying polarization. In this paper, we present star test polarimetry which is a method of polarization retrieval by analyzing a single frame point spread function. A trigonally stressed window placed at the pupil plane of an imaging system is used to produce point spread functions which are then processed to extract the polarization state of the incoming beam under investigation. We outline several methods which are used to recover the Stokes parameters of a beam of unknown polarization from the irradiance distribution of its point spread function.

关键词： Polarimetry microscopy Unconventional Polarization states

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Use of bessel beams and position-sensitive detectors in scanning photon microscope for improved field depth and contrast

Use of bessel beams and position-sensitive detectors in scan...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing xiiI

作者： Khmaladze, Alexander Kim, Myung K. Univ S Florida Dept Phys 4202 E Fowler Ave Tampa FL 33620 USA

ISBN: (纸本)0819461326

The scanning photon microscope technique is a method of microscopic image formation that employs a laser beam focused on a sample, while non-imaging detector receives the scattered light. The scans are achieved by means of a galvanometer based scanning mirror and a motorized micrometer. The system produces images analogous to the scanning electron microscopy with three-dimensional effects of shadowing and reflection. Compared to a conventional wide-field imaging system, the method allows for a greater ease of operation and flexibility, as the image quality is dependent upon the characteristics of the laser beam, rather than imaging optics. The image resolution on the order of a micron is demonstrated. A further gain in terms of resolution and the depth of focus by employment of Bessel rather than Gaussian beams is discussed. Additionally, we used a position-sensitive quadrant photodiode detector to highlight the overall spatial orientation of the imaged surface as well as its roughness. This concept can be useful in many areas, such as coherence imaging and fluorescence.

关键词： scanning microscopy axicon surface profile

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High resolution 3D-reconstruction from serial sections - Microscope instrumentation, software design and its implementations

High resolution 3D-reconstruction from serial sections - Mic...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing IX

作者： Verbeek, FJ Boon, PJ Netherlands Inst Dev Biol Hubrecht Lab NL-3584 CT Utrecht Netherlands

ISBN: (纸本)0819443603

This paper describes a set-up for high-resolution imaging with a conventional microscope that allows producing a 3D-image set from which a 3D model can be derived. The 3D-image set, is de facto, a gray value voxel model. In order to obtain such 3D image set a good administration needs to be maintained at acquisition and moreover, the acquisition must be realized in two steps. High-resolution images are built of image tiles and sophisticated algorithms are required to build a coherent image from the tiles.

关键词： image processing high-resolution image image tile image mosaic 3D reconstruction 3D image bioinformatics

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Wide-Field Time-Correlated Single Photon Counting imaging for luminescence microscopy

Wide-Field Time-Correlated Single Photon Counting imaging fo...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Sergent, Nicolas Levitt, James A. Green, Mark A. Suhling, Klaus Kings Coll London Dept Phys London WC2R 2LS England

ISBN: (纸本)9780819470362

We demonstrate Wide-Field Time-Correlated Single Photon Counting (WiFi TCSPC) imaging based on an image intensifier and a high-speed camera running at 30,000 frames per second. The timing of photon events is thus performed in parallel, simultaneously on every pixel. The system is applied to lanthanide lifetime measurements and time-resolved imaging of the lanthanide complex Europium Polyoxometalate (Eu POMs). We measure a lifetime of 2.98 ms for Eu POMs in solid state, which is in excellent agreement with the literature value.

关键词： Time-Correlated Single Photon Counting Lifetime Imaging microchannel plate TCSPC

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