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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

共 561 条记录，以下是151-160 订阅

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Focused beam scatterometry for deep subwavelength metrology

Focused beam scatterometry for deep subwavelength metrology

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Brown, Thomas G. Alonso, Miguel A. Vella, Anthony Theisen, Michael J. Head, Stephen T. Gillmer, Steven R. Ellis, Jonathan D. Univ Rochester Inst Opt Rochester NY 14627 USA Univ Rochester Dept Mech Engn Rochester NY 14627 USA

ISBN: (纸本)9780819498625

It is known that far-field scattered light requires a priori sample information in order to reconstruct nm-scale information such as is required in semiconductor metrology. We describe an approach to scatterometry that uses unconventional polarization states in the pupil of a high NA objective lens. We call this focused beam scatterometry;we will discuss the sensitivity limits to this approach and how it relates to micro-ellipsometry as well as low-NA scatterometry.

关键词： Spatial Light Modulators Polarization Control microscopy Pupil Engineering

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Experimental analysis of focal fields in laser scanning fluorescence stereomicroscopy

Experimental analysis of focal fields in laser scanning fluo...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Yang, Yanlong Peng, Tong Lei, Ming Li, Runze Zhou, Xing Wu, Di Yao, Baoli Ye, Tong Chinese Acad Sci Xian Inst Opt & Precis Mech State Key Lab Transient Opt & Photon Xian Shaanxi Peoples R China Clemson Univ Ctr Optic Mat Sci & Engn Technol Dept Bioengineering Clemson SC 29631 USA

ISBN: (纸本)9780819498625

Bessel beams have been proved to have the ability to extend the depth of focus in fluorescence microscopy. But the depth discrimination was not investigated thoroughly. Following our previous work(2), we investigated focal fields of Bessel-Gauss beams at different scanning angles. We found that the central focusing lines were tilted differently at different scanning angles. This effect manifests the ability of the true perspective view in the fluorescence stereomicroscopy.

关键词： Bessel-Gauss beams extended depth of field stereomicroscopy laser scanning three-dimensional imaging volume imaging

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Correction of image artifacts caused by refractive index gradients in scanning laser optical tomography

Correction of image artifacts caused by refractive index gra...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Antonopoulos, Georgios Christian Pscheniza, Dimitri Lorbeer, Raoul-Amadeus Heidrich, Marko Schwanke, Kristin Zweigerdt, Robert Ripken, Tammo Meyer, Heiko Laser Zentrum Hannover eV Biomed Opt Dept Hannover Germany LEBAO Hannover Med Sch Hannover Germany HTTG Dept Cardiothorac Transplantat & Vascular Surg Hannover Med Sch Hannover Germany

ISBN: (纸本)9780819498625

We present a technique for correcting image artifacts caused by refractive index distributions in Scanning Laser Optical Tomography (SLOT) and Optical Projection Tomography (OPT). Projection images can be distorted due to the presence of a refractive index distribution around the sample. We consider the special case of a refractive index distribution given by a capillary around a sample. The particular application we are interested in is in vitro imaging of cell spheroids in a glass capillary. Numerical simulations and experimental results are used to illustrate the connection between the Radon transform and the refracted projection. Thereupon we will describe a technique that transforms refracted projections to parallel ray Radon projections and thus allows artifact free reconstruction within the sample volume.

关键词： 3D fuorescence microscopy optical projection tomography scanning laser optical tomography refraction refractive index aberration correction artifact correction

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Further developments in addressing depth-variant 3D fluorescence microscopy imaging

Further developments in addressing depth-variant 3D fluoresc...

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Ghosh, Sreya Schaefer, Lutz Schuster, Dietwald Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Adv Imaging Methodol Consultat Kitchener ON Canada Regensburg Univ Appl Sci Regensburg Germany

ISBN: (纸本)9780819498625

Three-dimensional (3D) imaging with optical sectioning microscopy uses computational methods to obtain the true fluorescence distribution by ameliorating the effect of defocus, spherical aberration and noise. Inverse algorithms improve image quality at a fraction of the cost of implementing an optical system by accurate modeling of the imaging system. Good inverse imaging algorithms need to be accurate as well as fast. Better understanding of the image formation model is vital to obtain improved restoration through model-based algorithms. Forward imaging models based on a depth-varying point-spread function (DV-PSF) leads to a substantial improvement in the resulting images because it accounts for depth-induced aberrations present in the imaging system. PSFs at every layer can be represented using their principal components. Computation of the forward imaging model using a principal component analysis (PCA) representation of the DV-PSF requires fewer convolutions than a strata based approach investigated in the past. In this paper we present a new algorithm for maximum likelihood image restoration developed based on a PCA representation of the DV-PSF and an accelerated conjugate gradient (CG) iteration scheme. Results obtained with this PCA-CG algorithm from both simulated and experimental fluorescence microscope data are discussed and compared with results obtained from a CG iteration method based on the strata model and linear interpolation of the DV-PSF. The performance of the PCA-CG algorithms is shown to be promising for practical applications.

关键词： Point spread functions Principal component analysis 3D image processing Expectation maximization algorithms 3D modeling image restoration Algorithm development Computer simulations Luminescence Reconstruction algorithms

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Quantifying melanin distribution using pump-probe microscopy and a 2D morphological autocorrelation transformation for melanoma diagnosis

Quantifying melanin distribution using pump-probe microscopy...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Robles, Francisco E. Wilson, Jesse W. Warren, Warren S. Duke Univ Dept Chem Durham NC 27708 USA

ISBN: (纸本)9780819498625

Pump-probe microscopy is a quantitative molecular imaging technique that yields diagnostically relevant information from endogenous pigments, like melanin, by probing their ultrafast photodynamic properties. Previously, the method was applied to image thin, pigmented, cutaneous samples at different stages of melanoma, and results have shown a correlation between melanin photodynamic behavior and malignancy. Here, we add to the diagnostic power of the method by applying principles of mathematical morphology to parameterize melanins' image structure. Along with bulk melanin chemical information, results show that this method can differentiate invasive melanomas from non-invasive and benign lesions with high sensitivity and specificity (92.3% and 97.5%, respectively, with N = 53). The mathematical method and the statistical analysis are described in detail and results from cutaneous and ocular conjuctival melanocytic lesions are presented.

关键词： Melanoma microscopy Diagnostics Molecules Chemical analysis Modulation Optical parametric oscillators Statistical analysis Molecular imaging Ultrafast imaging

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Community detection for fluorescent lifetime microscopy image segmentation

Community detection for fluorescent lifetime microscopy imag...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Hu, Dandan Sarder, Pinaki Ronhovde, Peter Achilefu, Samuel Nussinov, Zohar Washington Univ Dept Phys One Brookings DrCampus Box 1105 St Louis MO 63130 USA Washington Univ Sch Med Dept Radiol St Louis MO 63110 USA

ISBN: (纸本)9780819498625

Multiresolution community detection (CD) method has been suggested in a recent work as an efficient method for performing unsupervised segmentation of fluorescence lifetime (FLT) images of live cell images containing fluorescent molecular probes. 1 In the current paper, we further explore this method in FLT images of e x vivo tissue slices. The image processing problem is framed as identifying clusters with respective average FLTs against a background or "solvent" in FLT imaging microscopy (FLIM) images derived using NIR fluorescent dyes. We have identified significant multiresolution structures using replica correlations in these images, where such correlations are manifested by information theoretic overlaps of the independent solutions ("replicas") attained using the multiresolution CD method from different starting points. In this paper, our method is found to be more efficient than a current state-of-the-art image segmentation method based on mixture of Gaussian distributions. It offers more than 1 : 25 times diversity based on Shannon index than the latter method, in selecting clusters with distinct average FLTs in NIR FLIM images.

关键词： image segmentation Fluorescence lifetime imaging Near infrared 3D image processing Critical dimension metrology Expectation maximization algorithms microscopy image processing algorithms and systems Tissues image resolution

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Investigation of the SQUBIC phase mask design for depth-invariant widefield microscopy point-spread function engineering

Investigation of the SQUBIC phase mask design for depth-inva...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Doblas, Ana King, Sharon V. Patwary, Nurmohammed Saavedra, Genaro Martinez-Corral, Manuel Preza, Chrysanthe Univ Valencia Dept Opt E-46100 Burjassot Spain Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA

ISBN: (纸本)9780819498625

Point-spread function engineering (PSF), achieved by placing a phase mask at the pupil plane of the imaging lens to encode the wavefront emerging from an imaging system, can be employed to reduce the impact of spherical aberration (SA) in 3D microscopy. In a previous study, the effect of SA on a confocal scanning microscope using a squared cubic phase mask (SQUBIC) was investigated using computer simulations. Here the effect of the SQUBIC design parameter on the insensitivity of the engineered PSF to SA is investigated using a metric based on the second-order moment of the axial variability of the PSF. We show that it is possible to find the optimum SQUBIC for the insensitization to SA.

关键词： Point-spread function engineering wavefront coding spherical aberration

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Customized Profile Lens Based Linear Response Fast Spinning On-axis Optical Scanners for 3D Laser Scanning microscopy

Customized Profile Lens Based Linear Response Fast Spinning ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Lai, Zhenhua Mega, Yair J. Yang, Xibin Zhu, Jianfeng Gu, Zetong Xiong, Daxi DiMarzio, Charles A. Northeastern Univ Dept Elect & Comp Engn Boston MA 02115 USA Northeastern Univ Dept Mech & Ind Engn Boston MA 02115 USA Chinese Acad Sci Suzhou Inst Biomed Engn & Technol Jiangsu Key Lab Med Optic Suzhou Peoples R China

ISBN: (纸本)9780819498625

Previously, we presented an on-axis linear-response linear-motion optical scanner. While the linear design is highly desired for engineering consideration, it was still lacking the scanning speed required for imaging applications. We here present a customized profile lens (CPL), tailored for high speed performance while maintaining the advantages of a linear response on-axis optical scanner. The device was built and tested experimentally on an optical bench. The test results demonstrate precise linear response and fast scanning speed, and revealed video frame rate scanning ability. The implementation of the CPLs in laser scanning systems is promising in improving the current 3D laser scanning microscopy systems by reducing the size, error, and complexity of the system, as well as other systems unitizing high speed laser scanning technique.

关键词： Beam Steering Laser Scanning microscopy Optical Scanner

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Full Field Photothermal Dynamics microscopy

Full Field Photothermal Dynamics Microscopy

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Tang, Guichen Kong, Fanting Chen, Y. C. Xu, M. Fairfield Univ Dept Phys 1073 North Benson Rd Fairfield CT 06824 USA Clemson Univ Ctr Opt Mat Sci & Engn Technol Clemson SC 29625 USA Univ New York Hunter Coll Dept Phys New York NY 10065 USA

ISBN: (纸本)9780819498625

We present here a novel full field pump-probe photothermal dynamics microscopy (PTDM) which uses a numerical lock-in mechanism for capturing full field photothermal responses and is capable of imaging 2D thermal dissipation dynamics by varying the time delay between the probing and pump nano-second pulses. PTDM may find interesting applications in biology and medicine. As one example, we report PTDM imaging nuclei contained on intact hematoxylin and eosin (H&E) stained prostate cancer specimens may potentially be used to distinguish low grade and high grade prostate cancer.

关键词： photothermal microscopy dynamics full-field microscope cancer diagnosis

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Reducing depth induced spherical aberration in 3D widefield fluorescence microscopy by wavefront coding using the SQUBIC phase mask

Reducing depth induced spherical aberration in 3D widefield ...

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Patwary, Nurmohammed Doblas, Ana King, Sharon V. Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Univ Valencia Dept Opt E-46100 Burjassot Spain

ISBN: (纸本)9780819498625

Imaging thick biological samples introduces spherical aberration (SA) due to refractive index (RI) mismatch between specimen and imaging lens immersion medium. SA increases with the increase of either depth or RI mismatch. Therefore, it is difficult to find a static compensator for SA(1). Different wavefront coding methods(2,3) have been studied to find an optimal way of static wavefront correction to reduce depth-induced SA. Inspired by a recent design of a radially symmetric squared cubic (SQUBIC) phase mask that was tested for scanning confocal microscopy(1) we have modified the pupil using the SQUBIC mask to engineer the point spread function (PSF) of a wide field fluorescence microscope. In this study, simulated images of a thick test object were generated using a wavefront encoded engineered PSF (WFE-PSF) and were restored using space-invariant (SI) and depth-variant (DV) expectation maximization (EM) algorithms implemented in the COSMOS software(4). Quantitative comparisons between restorations obtained with both the conventional and WFE PSFs are presented. Simulations show that, in the presence of SA, the use of the SIEM algorithm and a single SQUBIC encoded WFE-PSF can yield adequate image restoration. In addition, in the presence of a large amount of SA, it is possible to get adequate results using the DVEM with fewer DV-PSFs than would typically be required for processing images acquired with a clear circular aperture (CCA) PSF. This result implies that modification of a widefield system with the SQUBIC mask renders the system less sensitive to depth-induced SA and suitable for imaging samples at larger optical depths.

关键词： Three-dimensional microscopy wavefront encoding point-spread function engineering image restoration phase mask

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