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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

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Improved image-forming optics for transmission optical projection tomography

Improved image-forming optics for transmission optical proje...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Wang, Yi Wang, Ruikang Oregon Hlth & Sci Univ Dept Biomed Engn Beaverton OR 97006 USA Foshan Univ Dept Photoelect & Phys Foshan 528000 Peoples R China

ISBN: (纸本)9780819465566

We demonstrate an improved image-forming optics for transmission optical projection tomography (TOPT), with which the parallel integral throughout an object can be obtained. This is performed by introducing a shutter with an appropriate diameter at the back focus of the objective lens. We evaluate and compare the performances of the improved and the conventional TOPT systems with different parameters to obtain the optimal configuration. The optimal reconstruction is achieved by the improved configuration with alpha = 0.8 degrees similar to 1.6 degrees, and the spatial resolution reaches 25 mu n. The optimal configuration is validated by TOPT of a phantom sample and a five days chick embryo.

关键词： optical projection tomography point spread function parallel integral

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Tradeoff between insensitivity to depth-induced spherical aberration and resolution of 3D fluorescence imaging due to the use of wavefront encoding with a radially symmetric phase mask 25

Tradeoff between insensitivity to depth-induced spherical ab...

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conference on Three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Doblas, Ana Dutta, Ananya Saavedra, Genaro Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Univ Valencia Dept Opt E-46100 Burjassot Spain

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Previously, a wavefront encoded (WFE) imaging system implemented using a squared cubic (SQUBIC) phase mask has been verified to reduce the sensitivity of the imaging system to spherical aberration (SA). The strength of the SQUBIC phase mask and, as consequence, the performance of the WFE system are controlled by a design parameter, A. Although the higher the A-value, the more tolerant the WFE system is to SA, this is accomplished at the expense of the effective imaging resolution. In this contribution, we investigate this tradeoff in order to find an optimal A-value to balance the effect of SA and loss of resolution.

关键词： three-dimensional microscopy wavefront encoding point-spread function engineering resolution limit

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Improvement based on FDK reconstruction algorithm of conebeam CT

Improvement based on FDK reconstruction algorithm of conebea...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Miao, Hui Wang, Tingting Zhao, Huijuan Gao, Feng Tianjin Univ Coll Precis Instruments & Optoelect Engn Tianjin 300072 Peoples R China

ISBN: (纸本)9780819479662

As a typical 3D image algorithm, Feldkamp-David-Kress reconstruction algorithm is a filtered backprojection very similar to the 2D algorithm. Recent years, it has been widely used for its easy implementation and acceptable reconstruction precision for small cone-beam angle. For big cone-beam angles, however, the reconstruction exactness of horizontal planes will dramatically decrease with the distance from the central plane increases. Therefore, for improving the reconstruction precision in vertical direction, a similar reciprocal Gaussian function as the weighted factor was introduced into FDK algorithm in the article. The validity of the improved FDK algorithm was verified and evaluated through both the computer numerical simulation and the phantom model experiment. The quantitative analysis for the reconstruction results demonstrated that the reconstruction image using the improved FDK algorithm could primely revise the original reconstruction images and restore well closely to the tomography image of the prototype object.

关键词： FDK reconstruction cone-beam CT image reconstruction reconstruction algorithm

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Tissomics:: two- and threedimensional distribution of nuclei in brain tissue using Laser Scanning Cytometry (LSC)

Tissomics:: two- and threedimensional distribution of nuclei...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xii

作者： Lenz, D Mittag, A Mosch, B Bocsi, J Arendt, T Tárnok, A Univ Leipzig Heart Ctr Leipzig Leipzig Germany

ISBN: (纸本)0819456756

Automated quantitative (i.e. stochiometric) analysis of tissues is of eminent importance in the understanding of all interactions between cells in their natural environment. In tissue cytometry a solid trigger is necessary in order to unequivocally differentiate between cellular and non-cellular events. This can be best performed by nuclear staining. Aim of this study was to analyze a brain tissue section by laser scanning cytometry (LSC) in order to depict the threedimensional distribution of nuclei in the tissue. To this end the section was measured in several foci and different nuclei detected in several depths of the tissue were assigned to the respective layer. Frozen sections of formalin-fixed rat or human brain tissue (120 mu m thickness) were incubated with propidiumiodide (PI) (50 mu g/ml) and covered on slides. For analysis by the LSC propidiumiodide was used as trigger. After a first analysis focussed on the top of the tissue, the focus was adjusted in 30 mu m steps deeper into the tissue. Per analysis data of at least 50,000 cells were acquired. After finishing measurements from all depths of the field were merged, i.e. data were combined into a composite data file. With the special features of the LSC it was possible to develop a method depicting the threedimensional distribution of the nuclei in solid tissue sections. LSC can be useful tool for this relatively new field of solid tissue cytometry termed tissomics. After evaluation of methods like this, so far not available data can be analysed for diagnostic purposes. By these studies we intend to demonstrate the power of the LSC for the routine pathological use. This should add up to the bright versatility of applications for the LSC as a cytometric instrument suitable for high throughput and high content analysis.

关键词： Alzheimer's disease cell cycle DNA-polyploidy laser scanning cytometry cytomics

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3D imaging of the cleared intact murine colon with light sheet microscopy 13

3D imaging of the cleared intact murine colon with light she...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XxiiI

作者： Zufiria, B. Bocancea, D. I. Gomez-Gaviro, M. V. Vaquero, J. J. Desco, M. Fresno, M. Ripoll, J. Arranz, A. Univ Autonoma Madrid CSIC Ctr Mol Biol Severo Ochoa CBMSO Madrid Spain Univ Carlos III Madrid Dept Bioengn & Aerosp Engn Madrid 28911 Spain Inst Invest Sanitaria Gregorio Maranon Madrid 28007 Spain

ISBN: (纸本)9781628419474

We here show 3D light sheet microscopy images of fixed and cleared murine colon tissue in-toto, which offer relevant cellular information without the need for physically sectioning the tissue. We have applied the recently developed CUBIC protocol (Susaki et al. Cell 157: 726, 2014) for colon tissues and have found that this clearing protocol enables imaging all the way to the central part of the lumen with cellular resolution, thus opening new ways for 3D imaging of colon samples

关键词： light sheet microscopy CUBIC protocol tissue clearing

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Application of linear-scale differential analysis in phase correlation method of image stitching 13

Application of linear-scale differential analysis in phase c...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XxiiI

作者： Bezzubik, Vitalii V. Belashenkov, Nickolai R. Losev, Sergei V. ITMO Univ Kronverksky Pr 49 St Petersburg 197101 Russia

ISBN: (纸本)9781628419474

A robust method of creation of panoramic images which does not consume much processing resources is proposed and investigated. The phase correlation (PC) method is taken as a basic one because of simplicity of its algorithm and low computing time due to application of FFT technique. Standard PC procedure is modified by preprocessing of source frames of panoramic images in spatial domain. Preprocessing comprises Linear-Scale Differential Analysis (LSDA) with sequent content-dependent thresholding of intensity gradients. Method is proved for artificially blurred and noise corrupted images. It is shown that new robust algorithm allows to increase the productivity of creation of panoramic images keeping the probability of successive stitching close to maximum even for low-quality source frames.

关键词： Digital image image stitching phase correlation linear-scale differential analysis

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Development of a random access multi-photon microscope for fast three-dimensional functional recording of neuronal activity

Development of a random access multi-photon microscope for f...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Reddy, Duemani Saggau, Peter Rice Univ Dept Bioengn Houston TX 77251 USA Baylor Coll Med Dept Neurosci Houston TX 77030 USA

ISBN: (纸本)9780819465566

Over the past two decades, the dendritic processes of neurons have been shown to possess active and dynamic properties that give them the ability to modulate synaptic integration and shape individual synaptic responses. Effectively studying these properties at multiple locations on a live neuron in highly scattering brain. tissue requires an imaging/recording mechanism with high spatiotemporal resolution as well as optical sectioning and random access site selection capabilities. Our lab has made significant steps in developing such a system by combining the spatial resolution and optical sectioning ability of imaging techniques such as confocal and multi-photon microscopy with the temporal resolution and random access capability provided by acousto-optic laser scanning. However, all systems that have been developed to date restrict fast imaging to two-dimensional (2D) scan patterns. This severely limits the extent to which many neurons can be studied since they represent complex three-dimensional (3D) structures. We have previously demonstrated a scheme for fast 3D scanning which utilizes a unique arrangement of multiple acousto-optic deflectors and does not require axial movements of the objective lens. Here we couple this scanning scheme to a modified commercial research microscope and use the combined system to effectively image user-defined sites of interest on fluorescent 3D structures with positioning times that are in the low microsecond range. The resulting random-access scanning mechanism allows for functional imaging of complex 3D cellular structures such as neuronal dendrites at frames rates on the order of tens of kilohertz.

关键词： Neurons 3D scanning Microscopes 3D image processing Imaging systems Objectives Tissue optics Acousto-optics Confocal microscopy Laser scanners

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Single image structured illumination (SISIM) for in-vivo imaging 25

Single image structured illumination (SISIM) for in-vivo ima...

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conference on Three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Hoffman, Zachary R. Kivanc, Kose Di Marzio, Charles A. Northeastern Univ 360 Huntington Ave Boston MA 02115 USA Mem Sloan Kettering Canc Ctr 1275 York Ave New York NY 10021 USA

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Recently, it has been demonstrated that sectioning within a turbid media using structured illumination microscopy can be greatly enhanced through the use of a Hilbert transform algorithm. This technique, known as single image structured illumination microscopy (SISIM), provides high quality sectioning even within a strongly refracting/scattering medium, such as tissue. It is found that motion artifacts comprise a large portion of the error in in-vivo imaging. Thus, we expand on the SISIM technique by implementing an image registration algorithm which greatly mitigates motion induced artifacts. Multiple images and movies are developed to demonstrate SISIMs ability to provide real-time, in-vivo imaging of human tissue.

关键词： Structured Illumination optical sectioning thick specimen turbid media incoherent light image registration

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microscopy using source and detector arrays 13

Microscopy using source and detector arrays

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XxiiI

作者： Sheppard, Colin J. R. Castello, Marco Vicidomini, Giuseppe Duocastella, Marti Diaspro, Alberto Ist Italiano Tecnol Nanoscopy Nanophys Via Morego 30 I-16163 Genoa Italy Univ Genoa DIBRIS Via Opera Pia 13 I-16145 Genoa Italy

ISBN: (纸本)9781628419474

There are basically two types of microscope, which we call conventional and scanning. The former type is a full-field imaging system. In the latter type, the object is illuminated with a probe beam, and a signal detected. We can generalize the probe to a patterned illumination. Similarly we can generalize the detection to a patterned detection. Combining these we get a range of different modalities: confocal microscopy, structured illumination (with full-field imaging), spinning disk (with multiple illumination points), and so on. The combination allows the spatial frequency bandwidth of the system to be doubled. In general we can record a four dimensional (4D) image of a 2D object (or a 6D image from a 3D object, using an acoustic tuneable lens). The optimum way to directly reconstruct the resulting image is by image scanning microscopy (ISM). But the 4D image is highly redundant, so deconvolution-based approaches are also relevant. ISM can be performed in fluorescence, bright field or interference microscopy. Several different implementations have been described, with associated advantages and disadvantages. In two-photon microscopy, the illumination and detection point spread functions are very different. This is also the case when using pupil filters or when there is a large Stokes shift.

关键词： Confocal microscopy scanning optical microscopy fluorescence microscopy image scanning microscopy pixel reassignment

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Mueller polarimetric microscopy

Mueller polarimetric microscopy

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Laude-Boulesteix, B De Martino, A Le Naour, G Genestie, C Schwartz, L Garcia-Caurel, E Drévillon, B Ecole Polytech Phys Interfaces & Couches Minces Lab CNRS UMR 7647 F-91128 Palaiseau France

ISBN: (纸本)0819452327

We present a multispectral polarimetric imagine, system well suited for complete Mueller matrix microscopy. The source is a spectrally filtered halogen light bulb, and the image is formed on a fast CCD camera The light polarization is modulated before the sample and analyzed after the sample by using nematic liquid crystal modulators.. The whole Mueller matrix image of the sample is typically measured over 5 seconds for a good signal-to-noise ratio. The instrument design, together with an original and easy-to-operate calibration procedure provides a high polarimetric accuracy over wide ranges of wavelengths and magnifications. Mueller polarimetry provides separate images of scalar and vector retardation and dichroism of the sample, together with its depolarizing power, while all these effects do contribute simultaneously to the contrasts observed in standard polarized microsopy. Polarimetric images of several samples, namely an unstained rabbit cornea, a picrosirius red stained hepatic biopsy, and a rat artery specifically stained for collagen III are shown and discussed.

关键词： imaging systems polarimetry medical optics and biotechnology histology

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