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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

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A compact open-top light-sheet microscope for Optofluidic imaging 29

A compact open-top light-sheet microscope for Optofluidic im...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIX

作者： Son, Jeonghwan Mandracchia, Biagio Jia, Shu Georgia Inst Technol Wallace H Coulter Dept Biomed Engn Atlanta GA 30332 USA Emory Univ Atlanta GA 30332 USA

ISBN: (纸本)9781510648043;9781510648036

The fluorescence Optofluidic imaging have recently transformed conventional optical investigations of biological systems by exploiting the synergic combination of recent microscopy and integrated chips technologies. The implementation of light-sheet microscopy on Optofluidic imaging has further pushed the systemic capabilities towards fast and 3D-volumetric imaging. Here, we propose a new compact open-top light-sheet microscope to extend the study of 3D-fluorescence Optofluidic imaging. The proposed portable architecture of the system and customized reconstruction algorithms enable providing high throughput and volumetric reconstruction capability with an isometric single-cell resolution. The experimental results demonstrate the applicability of open-top light-sheet configuration to single-cell imaging in a flow. We anticipate the system to offer promising applications in clinical settings where the portability and 3D-volumetric analysis are required for fast diagnostics.

关键词： Light-sheet microscopy Open-top Light-sheet microscopy Portable microscopy Optofluidic Optofluidic imaging 3D-Volumetric imaging Volumetric imaging High-throughput imaging

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High resolution 3D-reconstruction from serial sections - Microscope instrumentation, software design and its implementations

High resolution 3D-reconstruction from serial sections - Mic...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing IX

作者： Verbeek, FJ Boon, PJ Netherlands Inst Dev Biol Hubrecht Lab NL-3584 CT Utrecht Netherlands

ISBN: (纸本)0819443603

This paper describes a set-up for high-resolution imaging with a conventional microscope that allows producing a 3D-image set from which a 3D model can be derived. The 3D-image set, is de facto, a gray value voxel model. In order to obtain such 3D image set a good administration needs to be maintained at acquisition and moreover, the acquisition must be realized in two steps. High-resolution images are built of image tiles and sophisticated algorithms are required to build a coherent image from the tiles.

关键词： image processing high-resolution image image tile image mosaic 3D reconstruction 3D image bioinformatics

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Star Test Polarimetry using Stress-Engineered Optical Elements

Star Test Polarimetry using Stress-Engineered Optical Elemen...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIX

作者： Ramkhalawon, Roshita Beckley, Amber M. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA

ISBN: (纸本)9780819488701

Stress-engineered optical elements have potential applications in snapshot polarimetry, in which a single irradiance image is used to measure a spatially varying polarization. In this paper, we present star test polarimetry which is a method of polarization retrieval by analyzing a single frame point spread function. A trigonally stressed window placed at the pupil plane of an imaging system is used to produce point spread functions which are then processed to extract the polarization state of the incoming beam under investigation. We outline several methods which are used to recover the Stokes parameters of a beam of unknown polarization from the irradiance distribution of its point spread function.

关键词： Polarimetry microscopy Unconventional Polarization states

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Performance evaluation of point-spread function engineering to reduce the impact of depth-induced aberrations on extended depth-of-field microscopy

Performance evaluation of point-spread function engineering ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIX

作者： Yuan, Shuai Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA

ISBN: (纸本)9780819488701

In this study, we evaluated a point-spread function (PSF) engineered using wave front encoding (WFE) and a generalized cubic phase mask (GCPM) design selected to reduce the impact of depth-induced spherical aberration (SA) on extended depth-of-field (EDOF) microscopy with high NA lenses. Mean-square-error based metrics computed from three-dimensional (3D) depth-variant WFE-PSFs with increasing amounts of SA were used to quantify the engineered PSF's sensitivity to SA and to compare it to the sensitivity of the cubic-phase mask (CPM) PSF traditionally used for EDOF microscopy. The potential performance of the engineered PSF with resilience to SA was further evaluated with simulations in which EDOF images of a 3D object were obtained by processing WFE images with and without SA. A qualitative and quantitative comparison of the EDOF images with the true object show that the WFE-PSF engineering with the selected GCPM design provides better performance in reducing the impact of SA. In addition, the GCPM-based EDOF images do not suffer from the known lateral shift of object features located away from the plane of focus encountered in traditional CPM-based EDOF images.

关键词： Extended depth-of-field microscopy point-spread function engineering wavefront encoding spherical aberration

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Use of bessel beams and position-sensitive detectors in scanning photon microscope for improved field depth and contrast

Use of bessel beams and position-sensitive detectors in scan...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing xiiI

作者： Khmaladze, Alexander Kim, Myung K. Univ S Florida Dept Phys 4202 E Fowler Ave Tampa FL 33620 USA

ISBN: (纸本)0819461326

The scanning photon microscope technique is a method of microscopic image formation that employs a laser beam focused on a sample, while non-imaging detector receives the scattered light. The scans are achieved by means of a galvanometer based scanning mirror and a motorized micrometer. The system produces images analogous to the scanning electron microscopy with three-dimensional effects of shadowing and reflection. Compared to a conventional wide-field imaging system, the method allows for a greater ease of operation and flexibility, as the image quality is dependent upon the characteristics of the laser beam, rather than imaging optics. The image resolution on the order of a micron is demonstrated. A further gain in terms of resolution and the depth of focus by employment of Bessel rather than Gaussian beams is discussed. Additionally, we used a position-sensitive quadrant photodiode detector to highlight the overall spatial orientation of the imaged surface as well as its roughness. This concept can be useful in many areas, such as coherence imaging and fluorescence.

关键词： scanning microscopy axicon surface profile

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Wide-Field Time-Correlated Single Photon Counting imaging for luminescence microscopy

Wide-Field Time-Correlated Single Photon Counting imaging fo...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Sergent, Nicolas Levitt, James A. Green, Mark A. Suhling, Klaus Kings Coll London Dept Phys London WC2R 2LS England

ISBN: (纸本)9780819470362

We demonstrate Wide-Field Time-Correlated Single Photon Counting (WiFi TCSPC) imaging based on an image intensifier and a high-speed camera running at 30,000 frames per second. The timing of photon events is thus performed in parallel, simultaneously on every pixel. The system is applied to lanthanide lifetime measurements and time-resolved imaging of the lanthanide complex Europium Polyoxometalate (Eu POMs). We measure a lifetime of 2.98 ms for Eu POMs in solid state, which is in excellent agreement with the literature value.

关键词： Time-Correlated Single Photon Counting Lifetime Imaging microchannel plate TCSPC

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Three-dimensional super-resolution imaging of single molecules by spectrally selective confocal microscopy.

Three-dimensional super-resolution imaging of single molecul...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VI

作者： Brakenhoff, GJ van Oijen, AM Kohler, J Muller, M Schmidt, J Univ Amsterdam Dept Mol Cytol BioCentrum Amsterdam NL-1098 SM Amsterdam Netherlands

ISBN: (纸本)0819430757

It is shown that based on spectrally selective excitation of individual molecules in the focus of a high NA lens together with position sensitive imaging sub-resolution imaging of three-dimensional structures can be realised. The feasibility of the idea is demonstrated with NA = 0.55 optics on a model system of pentacene molecules in p-terphenal host matrix.

关键词： three-dimensional superresolution fluorescence single molecule spectrally selective cryogenic

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Evaluation of a combined two-color phase plate forming three-dimensional dark holes in super resolution microscopy 27

Evaluation of a combined two-color phase plate forming three...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Nagai, Koumei Maruyama, Takashi Kodaira, Akira Kumagai, Hiroshi Bokor, Nandor Iketaki, Yoshinori NTT Adv Technol Corp 3-1 Morinosato Wakamiya Atsugi Kanagawa 2430124 Japan Kitasato Univ Sch Allied Hlth Sci Phys 1-15-1 Kitasato Sagamihara Kanagawa 2520373 Japan Budapest Univ Technol & Econ Dept Phys Budafoki Ut 8 H-1111 Budapest Hungary Olympus Corp 2-3 Kuboyama Cho Hachioji Tokyo 1928512 Japan

ISBN: (纸本)9781510632547

In this study, a combined two-color phase plate (CTPP) was designed for super-resolution microscopy based on up conversion fluorescence depletion (FD), fabricated, and evaluated. It is composed of two types of phase plates, a spiral phase plate and an annular phase plate. A two-color phase plate modulates the phase of the erase beam while maintaining the phase of the pump beam. SRM performed using the proposed CTPP is expected to enable super resolution in both the focal plane and in the optical axis direction. Despite its complex structure, a highly accurate CTPP was obtained by using the exposure and etching processes used in semiconductor manufacturing.

关键词： Super-resolution microscopy Phase modulation Fluorescence depletion Optical-axis resolution

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Fluorescence lifetime imaging using light emitting diodes

Fluorescence lifetime imaging using light emitting diodes

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Kennedy, Gordon T. Elson, Daniel S. Munro, Ian Poher, Vincent French, Paul M. W. Neil, Mark A. A. Imperial Coll London Blackett Lab S Kensington Campus London SW7 2AZ England Imperial Coll London Dept Bioengn London SW7 2AZ England

We demonstrate fluorescence lifetime imaging using cheap, high power light emitting diodes as excitation sources. Both time domain and frequency domain fluorescence lifetime imaging techniques have been implemented at... 详细信息

ISBN: (纸本)9780819465566

关键词： fluorescence lifetime imaging LED microscopy

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Novel contrast mechanism for label free super-resolution imaging 13

Novel contrast mechanism for label free super-resolution ima...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XxiiI

作者： Alexandrov, Sergey A. McGrath, James Subhash, Hrebesh Boccafoschi, Francesca Giannini, Cinzia Leahy, Martin Natl Univ Ireland Sch Phys Tissue Opt & Microcirculat Imaging Grp Galway Ireland Univ Piemonte Orientale Dept Hlth Sci I-28100 Novara Italy CNR Inst Crystallog Via Amendola 122-O I-70126 Bari Italy

ISBN: (纸本)9781628419474

We present a novel super-resolution spectral encoding of spatial frequency (srSESF) approach to break the diffraction limit and dramatically improve resolution in lateral direction. The idea is to utilize additional information about the internal structure of the object to resolve features in the lateral direction. The novel contrast mechanism is realized by reconstruction and comparison of the axial spatial frequency (period) profiles at each image point to form superresolution image. As a result, small features, unresolved by conventional microscopy, can be visualized. Numerical simulation and experiments confirm the super-resolution abilities of the srSESF approach without applying any labels.

关键词： Optical microscopy label-free imaging super-resolution

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