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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

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New Light Field Camera based on Physical Based Rendering Tracing

New Light Field Camera based on Physical Based Rendering Tra...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Chung, Ming-Han Chang, Shan-Ching Lee, Chih-Kung Natl Taiwan Univ Inst Appl Mech Taipei Taiwan

ISBN: (纸本)9780819498625

Even though light field technology was first invented more than 50 years ago, it did not gain popularity due to the limitation imposed by the computation technology. With the rapid advancement of computer technology over the last decade, the limitation has been uplifted and the light field technology quickly returns to the spotlight of the research stage. In this paper, PBRT (Physical Based Rendering Tracing) was introduced to overcome the limitation of using traditional optical simulation approach to study the light field camera technology. More specifically, traditional optical simulation approach can only present light energy distribution but typically lack the capability to present the pictures in realistic scenes. By using PBRT, which was developed to create virtual scenes, 4D light field information was obtained to conduct initial data analysis and calculation. This PBRT approach was also used to explore the light field data calculation potential in creating realistic photos. Furthermore, we integrated the optical experimental measurement results with PBRT in order to place the real measurement results into the virtually created scenes. In other words, our approach provided us with a way to establish a link of virtual scene with the real measurement results. Several images developed based on the above-mentioned approaches were analyzed and discussed to verify the pros and cons of the newly developed PBRT based light field camera technology. It will be shown that this newly developed light field camera approach can circumvent the loss of spatial resolution associated with adopting a micro-lens array in front of the image sensors. Detailed operational constraint, performance metrics, computation resources needed, etc. associated with this newly developed light field camera technique were presented in detail.

关键词： Physical Based Rendering Tracing PBRT Light field Ray tracing image processing

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image acquisition, processing and reconstruction in holographic and tomographic diffractive microscopy

Image acquisition, processing and reconstruction in holograp...

引用

Digital Holography and Three-dimensional Imaging, DH 2014

作者： Bailleul, Jonathan Liu, Hui Simon, Bertrand Debailleul, Matthieu Haeberlé, Olivier Laboratoire MIPS EA-2332 Université de Haute-Alsace 61 rue Albert Camus 68093 Mulhouse Cedex France

ISBN: (纸本)9781557523082

Tomographic diffractive microscopy allows for imaging unlabeled specimens, with a better resolution than conventional microscopes, giving access to the index of refraction distribution within the specimen, and possibly at high speed. Principles of image formation and reconstruction are presented, and hint towards real-time, three-dimensional acquisition, image reconstruction and final display, are discussed. © 2014 OSA.

关键词： image reconstruction

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The Gray Institute Open Microscopes applied to radiobiology and protein interaction studiesZ

The Gray Institute Open Microscopes applied to radiobiology ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Barber, P. R. Tullis, I. D. C. Rowley, M. I. Martins, C. D. Weitsman, G. Lawler, K. Coffey, M. Woodman, N. Gillett, C. E. Ng, T. Vojnovic, B. Univ Oxford Gray Inst Radiat Oncol & Biol Dept Oncol Oxford OX1 2JD England Inst Math & Molecular Biomed Kings Coll London London England Kings Coll London Div Cancer Studies London England Kings Coll London Randall Div London England Kings Coll London Breakthrough Breast Cancer Res Unit London England Kings Coll London Guys & St Thomas Breast Tissue & Data Bank London England

ISBN: (纸本)9780819498625

We describe an 'open' design methodology for wide-field fluorescence, confocal and fluorescence lifetime imaging microscopy (FLIM), and how the resulting microscopes are being applied to radiation biology and protein activity studies in cells and human tissue biopsies. The design approach allows easy expansion as it moves away from the use of a monolithic microscope body to small, commercial off-the-shelf and custom made modular components. Details have been made available under an open license for non-commercial use at http://***/similar to atdgroup. Two radiobiology 'end-stations' have been constructed which enable fast radiation targeting and imaging of biological material opening up completely novel studies, where the consequences of ionising radiation (signaling and protein recruitment) can be studied in situ, at short times following irradiation. One is located at Surrey University, UK, where radiation is a highly focused in beam (e.g. protons, helium or higher mass ions). The second is installed at the Gray Institute linear accelerator facility, Oxford University, which uses sub-microsecond pulses of 6 MeV electrons. FLIM capabilities have enhanced the study of protein-protein interactions in cells and tissues via Frster Resonance Energy Transfer (FRET). Extracting FRET signals from breast cancer tissue is challenging because of endogenous and fixation fluorescence;we are investigating novel techniques to measure this robustly. Information on specific protein interactions from large numbers of patient tumors will reveal prognostic and diagnostic information.

关键词： Tissues Fluorescence resonance energy transfer Microscopes image filtering Atrial fibrillation Fluorescence lifetime imaging Optical filters Proteins Luminescence Linear filtering

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A high performance biometric signal and image processing method to reveal blood perfusion towards 3D oxygen saturation mapping

A high performance biometric signal and image processing met...

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conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues xii

作者： Imms, Ryan Hu, Sijung Azorin-Peris, Vicente Trico, Michael Summers, Ron Univ Loughborough Sch Elect Elect & Syst Engn Ashby Rd Loughborough LE11 3TU Leics England Paris Sud Univ Polytech Paris Sud Engn Sch F-91440 Orsay France

ISBN: (纸本)9780819498601

Non-contact imaging photoplethysmography (PPG) is a recent development in the field of physiological data acquisition, currently undergoing a large amount of research to characterize and define the range of its capabilities. Contact-based PPG techniques have been broadly used in clinical scenarios for a number of years to obtain direct information about the degree of oxygen saturation for patients. With the advent of imaging techniques, there is strong potential to enable access to additional information such as multi-dimensional blood perfusion and saturation mapping. The further development of effective opto-physiological monitoring techniques is dependent upon novel modelling techniques coupled with improved sensor design and effective signal processing methodologies. The biometric signal and imaging processing platform (bSIPP) provides a comprehensive set of features for extraction and analysis of recorded iPPG data, enabling direct comparison with other biomedical diagnostic tools such as ECG and EEG. Additionally, utilizing information about the nature of tissue structure has enabled the generation of an engineering model describing the behaviour of light during its travel through the biological tissue. This enables the estimation of the relative oxygen saturation and blood perfusion in different layers of the tissue to be calculated, which has the potential to be a useful diagnostic tool.

关键词： imaging photoplethysmography pulse oximetry oxygen saturation mapping signal acquisition and processing

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Hyperspectral holographic fourier-microscopy

Hyperspectral holographic fourier-microscopy

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Digital Holography and Three-dimensional Imaging, DH 2014

作者： Kalenkov, G.S. Kalenkov, S.G. Shtanko, A.E. 141700 9 Institutskiy per Dolgoprudny Moscow Region Russia 107023 B. Semenovskaya 38 Moscow Russia Moscow State University of Technology 'Stankin' 127994 Vadkovskiy per. 1 Moscow Russia

New lens free holographic image acquisition method in polychromatic, unpolarized light is presented. Hyperspectral amplitude and average-phase profile images of standard microscope samples obtained experimentally are ... 详细信息

ISBN: (纸本)9781557523082

关键词： Noise abatement

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Three-dimensional surface reconstruction by combining a pico-digital projector for structured light illumination and an imaging system with high magnification and high depth of field.

Three-dimensional surface reconstruction by combining a pico...

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conference on Optical Micro- and Nanometrology V

作者： Leong-Hoi, A. Serio, B. Twardowski, P. Montgomery, P. UDS CNRS UMR 7357 Lab Sci Ingenieur Informat & Imagerie ICube 300 Bd Sebastien BrantCS 10413 F-67412 Illkirch Graffenstaden France LEME F-92410 Paris France

ISBN: (纸本)9781628410808

Based on a miniature digital light projector (pico-DLP), a prototype of a Structured Illumination Microscope (SIM) has been developed. The pico-DLP is used to project fringes onto a sample and applying the three-step phase shifting algorithm together with the absolute phase retrieval method, the 3D shape of the object surface is extracted. By using a specific optical system instead of a conventional microscope objective, the device allows 3D reconstructions of surfaces with both a 10x magnification and a high depth of field obtained thanks to a small numerical aperture of 0.06 offering an acceptable lateral resolution of 6.2 mu m. An image processing algorithm has been developed to reduce the noise in the acquired images before applying the reconstruction algorithm and so optimize the reconstruction method. Compared with interference microscopy and confocal microscopy that have a shallower depth of field per XY image, the microscope developed achieves a depth of field about 700 mu m and requires no vertical scanning, which greatly reduces the acquisition time. Although the system at this stage does not have the same resolution performance as interference microscopy, it is nonetheless faster and cheaper. One possible application of this SIM technique would be to first reconstruct in real-time parts of an object before performing higher resolution 3D measurements with interference microscopy. As with all classical optical instruments, the lateral resolution is limited by diffraction. Work is being carried out with the prototype SIM system to be able to exceed the lateral resolution limits and thus achieve super resolution.

关键词： Structured Illumination microscopy (SIM) Mini projector Pico DLP 3D surface reconstruction Phase shifting

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Phase imaging with partially coherent light

Phase imaging with partially coherent light

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Waller, Laura Univ Calif Berkeley Dept Elect Engn & Comp Sci Berkeley CA 94702 USA

We review several quantitative phase imaging methods that retrieve phase from a through-focus series of intensity images (specifically, iterative methods and Transport of Intensity) and compare their performance when ... 详细信息

ISBN: (纸本)9780819493583

关键词： Phase imaging partial coherence microscopy transport of intensity

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A new expanded point information content design approach for 3D live-cell microscopy at video rates

A new expanded point information content design approach for...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Zahreddine, Ramzi N. Cormack, Robert H. Cogswell, Carol J. Univ Colorado Dept Elect Comp & Energy Engn Boulder CO 80309 USA

ISBN: (纸本)9780819493583

Through a combination of optical design and algorithm development, a new expanded point information content ( EPIC) microscope has been developed that is capable of extending the depth of field while simultaneously super locating the depth position of complex biological objects to within an accuracy of 75 nm. The data is then combined to form 3D animations of live-cell biological specimens. This is accomplished without the need to acquire multi-focal image stacks and is thus well suited for high-speed imaging.

关键词： 4D microscopy PSF engineering extended depth of field quantitative depth ranging computational optics live-cell imaging fluorescence microscopy

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Digital holographic confocal microscope

Digital holographic confocal microscope

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Goy, Alexandre S. Psaltis, Demetri Ecole Polytech Fed Lausanne CH-1015 Lausanne Switzerland

ISBN: (纸本)9780819493583

We demonstrate experimentally a scanning confocal microscopy technique based on digital holographic recording of the scanned spot. The data collected in this way contains all the information to produce three-dimensional images. Several methods to treat the data are presented, such as the dynamic placement of the pinhole. Examples of reflection and transmission images of epithelial cells and mouse brain tissue are shown. The computations can be performed in real time, the speed being limited only by the frame rate of the camera. This method enables a convenient implementation of confocal microscopy, especially in transmission as no de-scan device is required.

关键词： digital holography confocal microscopy three-dimensional imaging

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GPU-based image Registration in Aperture Correlation microscopy, and Reflection Mode Correlation microscopy

GPU-based Image Registration in Aperture Correlation Microsc...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Fafchamps, Lionel J. Neil, Mark A. A. Juskaitis, Rimas Imperial Coll London London England Aurox Ltd Abingdon Oxon England

ISBN: (纸本)9780819493583

Aperture Correlation microscopy (ACM) is a fluorescence microscopy technique capable of depth resolved imaging and enhanced lateral resolution at real-time acquisition rates. It relies on the subtraction of 2 separate images from different cameras which must be registered to the sub-pixel level. In order to achieve real-time registration and subtraction, the graphics processing unit (GPU) is used to apply a transformation from one frame to the other, resulting in a system capable of processing over 200 frames per second on modest hardware (GeForce 330M). Currently, this rate is limited by camera acquision to 16fps. Additionally, a novel reflection mode correlation microscope is introduced which functions on similar principles as the fluorescent system but can be used to examine reflective samples. images and z-stacks taken with this system are presented here.

关键词： Cameras microscopy image registration Microscopes Confocal microscopy image processing Objectives Polarizers image transmission Reflectivity

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