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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

共 561 条记录，以下是181-190 订阅

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Fuzzy Logic Components for Iterative Deconvolution Systems

Fuzzy Logic Components for Iterative Deconvolution Systems

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Northan, Brian M. True North Image Proc Guilderland NY 12084 USA

ISBN: (纸本)9780819493583

Deconvolution systems rely heavily on expert knowledge and would benefit from approaches that capture this expert knowledge. Fuzzy logic is an approach that is used to capture expert knowledge rules and produce outputs that range in degree. This paper describes a fuzzy-deconvolution-system that integrates traditional Richardson-Lucy deconvolution with fuzzy components. The system is intended for restoration of 3D widefield images taken under conditions of refractive index mismatch. The system uses a fuzzy rule set for calculating sample refractive index, a fuzzy median filter for inter-iteration noise reduction, and a fuzzy rule set for stopping criteria.

关键词： Deconvolution Fuzzy Logic Spherical Aberration

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Wide-Field microscopy using Microcamera Arrays

Wide-Field Microscopy using Microcamera Arrays

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Marks, Daniel L. Youn, Seo Ho Son, Hui S. Kim, Jungsang Brady, David J. Duke Univ Dept Elect & Comp Engn Durham NC 27708 USA

ISBN: (纸本)9780819493583

A microcamera is a relay lens paired with image sensors. Microcameras are grouped into arrays to relay overlapping views of a single large surface to the sensors to form a continuous synthetic image. The imaged surface may be curved or irregular as each camera may independently be dynamically focused to a different depth. Microcamera arrays are akin to microprocessors in supercomputers in that both join individual processors by an optoelectronic routing fabric to increase capacity and performance. A microcamera may image ten or more megapixels and grouped into an array of several hundred, as has already been demonstrated by the DARPA AWARE Wide-Field program with multiscale gigapixel photography. We adapt gigapixel microcamera array architectures to wide-field microscopy of irregularly shaped surfaces to greatly increase area imaging over 1000 square millimeters at resolutions of 3 microns or better in a single snapshot. The system includes a novel relay design, a sensor electronics package, and a FPGA-based networking fabric. Biomedical applications of this include screening for skin lesions, wide-field and resolution-agile microsurgical imaging, and microscopic cytometry of millions of cells performed in situ.

关键词： Wide-field microscopy camera array image processing agile resolution

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Intensity-based segmentation and visualization of cells in 3D microscopic images using the GPU

Intensity-based segmentation and visualization of cells in 3...

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Kang, Mi-Sun Lee, Jeong-Eom Jeon, Woong-Ki Choi, Heung-Kook Kim, Myoung-Hee Ewha Womans Univ Dept Comp Sci & Engn Seoul 120750 South Korea Inje Univ UHRC Sch Comp Engn Gyeong 621749 South Korea

ISBN: (纸本)9780819493583

3D microscopy images contain abundant astronomical data, rendering 3D microscopy image processing time-consuming and laborious on a central processing unit (CPU). To solve these problems, many people crop a region of interest (ROI) of the input image to a small size. Although this reduces cost and time, there are drawbacks at the image processing level, e. g., the selected ROI strongly depends on the user and there is a loss in original image information. To mitigate these problems, we developed a 3D microscopy image processing tool on a graphics processing unit (GPU). Our tool provides efficient and various automatic thresholding methods to achieve intensity-based segmentation of 3D microscopy images. Users can select the algorithm to be applied. Further, the image processing tool provides visualization of segmented volume data and can set the scale, transportation, etc. using a keyboard and mouse. However, the 3D objects visualized fast still need to be analyzed to obtain information for biologists. To analyze 3D microscopic images, we need quantitative data of the images. Therefore, we label the segmented 3D objects within all 3D microscopic images and obtain quantitative information on each labeled object. This information can use the classification feature. A user can select the object to be analyzed. Our tool allows the selected object to be displayed on a new window, and hence, more details of the object can be observed. Finally, we validate the effectiveness of our tool by comparing the CPU and GPU processing times by matching the specification and configuration.

关键词： GPU CUDA 3D microscopic image processing visualization

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High speed phase distortion measurement and compensation for focusing in space and time

High speed phase distortion measurement and compensation for...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Fiolka, Reto Cui, Meng Howard Hughes Med Inst Ashburn VA 20147 USA

ISBN: (纸本)9780819493583

Random scattering and aberrations severely limit the imaging depth in optical microscopy. We introduce a rapid, parallel wavefront compensation technique that efficiently compensates even highly complex phase distortions. Using coherence gated backscattered light as a feedback signal, we focus light deep inside highly scattering brain tissue. We demonstrate that the same wavefront optimization technique can also be used to compensate spectral phase distortions in ultrashort laser pulses using nonlinear iterative feedback. We can restore transform limited pulse durations at any selected target location and compensate for dispersion that has occurred in the optical train and within the sample.

关键词： Fluorescence microscopy adaptive optics imaging through turbid media ultrafast optics

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Time-resolved wide-field optically sectioned fluorescence microscopy

Time-resolved wide-field optically sectioned fluorescence mi...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Dupuis, Guillaume Benabdallah, Nadia Chopinaud, Aurelien Mayet, Celine Leveque-Fort, Sandrine Univ Paris 11 CPBM Federat LUMAT FR 2764 Orsay France CNRS Orsay France Univ Paris 11 Inst Sci Mol Orsay UMR 8214 Orsay France

ISBN: (纸本)9780819493583

We present the implementation of a fast wide-field optical sectioning technique called HiLo microscopy on a fluorescence lifetime imaging microscope. HiLo microscopy is based on the fusion of two images, one with structured illumination and another with uniform illumination. Optically sectioned images are then digitally generated thanks to a fusion algorithm. HiLo images are comparable in quality with confocal images but they can be acquired faster over larger fields of view. We obtain 4-D imaging by combining HiLo optical sectioning, time-gated detection, and z-displacement. We characterize the performances of this set-up in terms of 3-D spatial resolution and time-resolved capabilities in both fixed- and live-cell imaging modes.

关键词： fluorescence microscopy fluorescence lifetime imaging microscopy (FLIM) structured illumination microscopy (HiLo) wide-field optical sectioning

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Comparison of computational methods developed to address depth-variant imaging in fluorescence microscopy

Comparison of computational methods developed to address dep...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Rahman, Muhammad Mizanur Schaefer, Lutz H. Schuster, Dietwald Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Adv Imaging Mathodol Consultat Ontario ON Canada Univ Appl Sci Dept Math Regensburg Germany

ISBN: (纸本)9780819493583

In three-dimensional microscopy, the image formation process is inherently depth variant (DV) due to the refractive index mismatch between the imaging layers. In this study, we present a quantitative comparison among different image restoration techniques developed based on a depth-variant (DV) imaging model for fluorescence microscopy. The imaging models employed by these methods approximate DV imaging by either stratifying the object space (analogous to the discrete Fourier transform (DFT) "overlap-add" method) or image space (analogous to the DFT "overlap-save" method). We compare DV implementations based on maximum likelihood (ML) estimation and a previously developed expectation maximization algorithm to a ML conjugate gradient algorithm, using both these stratification approaches in order to assess their impact on the restoration methods. Simulations show that better restoration results are achieved with iterative methods implemented using the overlap-add method than with their implementation using the overlap-save method. However, the overlap-save method makes it possible to implement a non-iterative DV inverse filter that can trade off accuracy of the achieved result for computational speed. Results from a non-iterative regularized inverse filtering approach are also presented.

关键词： Depth-variant imaging 3D fluorescence microscopy spherical aberration image de-blurring

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Light sheet adaptive optics microscope for 3D live imaging

Light sheet adaptive optics microscope for 3D live imaging

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Bourgenot, C. Taylor, J. M. Saunter, C. D. Girkin, J. M. Love, G. D. Univ Durham Dept Phys Ctr Adv Instrumentat Durham DH1 3LE England

ISBN: (纸本)9780819493583

We report on the incorporation of adaptive optics (AO) into the imaging arm of a selective plane illumination microscope (SPIM). SPIM has recently emerged as an important tool for life science research due to its ability to deliver high-speed, optically sectioned, time-lapse microscope images from deep within in vivo selected samples. SPIM provides a very interesting system for the incorporation of AO as the illumination and imaging paths are decoupled and AO may be useful in both paths. In this paper, we will report the use of AO applied to the imaging path of a SPIM, demonstrating significant improvement in image quality of a live GFP-labeled transgenic zebrafish embryo heart using a modal, wavefront sensorless approach and a heart synchronization method. These experimental results are linked to a computational model showing that significant aberrations are produced by the tube holding the sample in addition to the aberration from the biological sample itself.

关键词： Optical microscopy Aberration correction Adaptive Optics Light sheet microscopy zebra fish

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A comparison of methods for optical sectioning using structured illumination microscopy

A comparison of methods for optical sectioning using structu...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Thomas, Benjamin Momany, Michelle Kner, Peter Univ Georgia Coll Engn Athens GA 30602 USA Univ Georgia Dept Plant Biol Athens GA 30602 USA

ISBN: (纸本)9780819493583

Structured Illumination microscopy is a simple and effective method to remove out-of-focus light in widefield fluorescence microscopy. Neil et al. originally proposed a simple square-law method for calculating the optically sectioned image from the three raw images with the structured illumination pattern super-imposed. However, the Neil method does not make the most efficient use of the three raw images. The three structured illumination images can also be used to separate three copies of the image covering shifted regions of frequency space in a similar manner to that developed by Gustafsson et al. These can then be combined using a generalized Wiener filter to create an image with a well-behaved optical transfer function in which the missing cone has been filled in, providing optical sectioning. Here, we compare the Neil and Gustafsson methods and show that the Gustafsson method provides an image with higher fidelity and a better Signal to Noise Ratio (SNR) at low photon counts. We apply the two methods to images of fluorescent beads and GFP labeled septins in Aspergillus nidulans.

关键词： fluorescence microscopy structured illumination microscopy three-dimensional imaging bioimaging

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Tomographic incoherent phase imaging, a diffraction tomography alternative for any white-light microscope

Tomographic incoherent phase imaging, a diffraction tomograp...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Bon, Pierre Aknoun, Sherazade Savatier, Julien Wattellier, Benoit Monneret, Serge Aix Marseille Univ Inst Fresnel Ecole Cent Marseille CNRSUMR7249 Domaine Univ St Jerome F-13397 Marseille France CNRS ESPCI UMR 7587 F-75005 Paris France PHSICS SA Campus Ecole Polytech F-91128 Palaiseau France

ISBN: (纸本)9780819493583

In this paper, we discuss the possibility of making tomographic reconstruction of the refractive index of a microscopic sample using a quadriwave lateral shearing interferometer, under incoherent illumination. A Z-stack is performed and the acquired incoherent elecromagnetic fields are deconvoluted before to retrieve in a quantitative manner the refractive index. The results are presented on polystyrene beads and can easily be expanded to biological samples. This technique is suitable to any white-light microscope equipped with nanometric Z-stack module.

关键词： Wavefront sensor Quantitative phase microscopy Diffraction Tomography Unlabeled biological samples

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Adapting phasor analysis for nonlinear pump-probe microscopy

Adapting phasor analysis for nonlinear pump-probe microscopy

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Robles, Francisco E. Wilson, Jesse W. Fischer, Martin C. Warren, Warren S. Duke Univ Dept Chem Durham NC 27708 USA

ISBN: (纸本)9780819493583

Phasor analysis has become a powerful tool for examining signals in fluorescence life-time microscopy (FLIM), where the analysis provides a fast, robust and intuitive means of separating different fluorescent species and mixtures thereof. In this work we adapt this analysis for pump-probe microscopy, a method that provides molecular contrast of pigmented samples by probing their excited state dynamic properties. The bipolar nature of the pump-probe signals presents important differences in the resulting phasors compared to FLIM-here, we discuss these differences and describe the behavior of bipolar signals in phasor analysis. Results show that this method is indeed able to separate multiple molecular species of interests and allows facile assessment of pigment chemistry and its distribution in samples.

关键词： microscopy Molecules Terahertz radiation Signal analyzers Statistical analysis Chemistry Fluorescence lifetime imaging Biological research Laser beam diagnostics Modulation

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