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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

共 561 条记录，以下是201-210 订阅

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Effects of a pupil filter on stimulated emission depletion microscopy

Effects of a pupil filter on stimulated emission depletion m...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing xiiI

作者： Yoo, Hongki Song, Incheon Kim, Taehoon Gweon, Daegab Korea Adv Inst Sci & Technol Dept Mech Engn Lab Nano Optomechatron Taejon 305701 South Korea Hanyang Univ Dept Biomed Engn Hanyang Peoples R China

ISBN: (纸本)0819461326

Recently, stimulated emission depletion microscopy has achieved high resolution in fluorescent imaging. In this paper, we present effects of a pupil filter on the performances of stimulated emission depletion microscopy. In stimulated emission depletion microscopy, a saturated zero-centered spot is usually used to achieve a high lateral resolution. Using a half-coated phase plate, a zero-centered spot was made with a narrow and steep gap at the center. Numerical and experimental results show that by simply inserting a central obstacle as a pupil filter, it is possible to reduce the central gap of the zero-centered spot. However in order to compensate inevitable loss of light, which is blocked by the obstacle, increased laser power is required.

关键词： STED microscopy confocal microscopy high resolution pupil filter phase plate annular aperture

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LABEL-FREE CLASSIFICATION OF CELL TYPES BY IMAGING OF CELL MEMBRANE FLUCTUATIONS USING LOW-COHERENT FULL-FIELD QUANTITATIVE PHASE microscopy

LABEL-FREE CLASSIFICATION OF CELL TYPES BY IMAGING OF CELL M...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Yamauchi, Toyohiko Sugiyama, Norikazu Sakurai, Takashi Iwai, Hidenao Yamashita, Yutaka Hamamatsu Photon KK Hamakita Ku 5000 Hirakuchi Hamamatsu Shizuoka 4348601 Japan MIT Spectroscopy Lab Cambridge MA 02139 USA Hamamatsu Univ Sch Med Photon Med Res Ctr Hamamatsu Shizuoka 4313192 Japan

ISBN: (纸本)9780819479662

Cell membrane motions of living cells are quantitatively measured in nanometer resolution by low-coherent full-field quantitative phase microscopy. Our setup is based on a full-field phase shifting interference microscope with a very low-coherent light source. The reflection mode configuration and the low-coherent illumination make it possible to differentiate the weak reflection light from the cell membrane from the strong reflection from the glass substrate. Two cell populations are quantitatively assessed by the power spectral density of the cell surface motion and show different trends.

关键词： quantitative phase microscope cell imaging plasma membrane

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Supercritical self-interference fluorescence microscopy for full-field membrane imaging

Supercritical self-interference fluorescence microscopy for ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Barroca, Thomas Bon, Pierre Leveque-Fort, Sandrine Fort, Emmanuel ESPCI ParisTech Inst Langevin Ctr Imageries Plasmon Appl CNRSUMR 7587 1 Rue Jussieu F-75231 Paris 05 France Univ Paris Sud CNRS CLUPS UMR 8214 F-91405 Orsay France

ISBN: (纸本)9780819493583

We present a new technique based on the self-interference of Supercritical Angle Fluorescence (SAF) emission in order to perform full-field cell membrane imaging. We show that our Point Spread Function (PSF) engineering technique allows us to obtain a 100 nm axial sectioning while conserving the original lateral resolution of the microscope. The images are acquired using an optical module that can be connected to any fluorescent microscope to simultaneously monitor in real time both the cell membrane and in-depth phenomena.

关键词： Cell membrane imaging Supercritical Angle Fluorescence (SAF) Fluorescence microscopy Dictyostelium discoideum

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Fabrication and characterization of novel microsphere-embedded optical devices for enhancing microscopy resolution 25

Fabrication and characterization of novel microsphere-embedd...

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conference on Three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Darafsheh, Arash Washington Univ Sch Med Dept Radiat Oncol St Louis MO 63110 USA

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Microsphere-assisted imaging can be incorporated onto conventional light microscopes allowing wide-field and flourescence imaging with enhanced resolution. We demonstrated that imaging of specimens containing sub diffraction-limited features is achievable through high-index microspheres embedded in a transparent thin film placed over the specimen. We fabricated novel microsphere-embedded microscope slides composed of barium titanate glass microspheres (with diameter 10-100 lam and refractive index-4.9-2.2) embedded in a transparent polydimethylsiloxane (PDMS) elastomer layer with controllable thickness. We characterized the imaging performance of such microsphere-embedded devices in white-light microscopies, by measuring the imaging resolution, field-of-view, and magnification as a function of microsphere size. Our results inform on the design of novel optical devices, such as microsphere-embedded microscope slides for imaging applications.

关键词： Super-resolution microscopy nanoscopy microsphere barium titanate PDMS

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Super-resolution optical microscopy by using dielectric microwires 13

Super-resolution optical microscopy by using dielectric micr...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XxiiI

作者： Darafsheh, Arash Wu, Gaoxiang Yang, Shu Finlay, Jarod C. Univ Penn Dept Radiat Oncol Philadelphia PA 19104 USA Univ Penn Dept Mat Sci & Engn 3231 Walnut St Philadelphia PA 19104 USA

ISBN: (纸本)9781628419474

We demonstrate that super-resolution imaging of specimens containing sub-diffraction-limited features is feasible by using dielectric microwires fabricated through capillary force lithography followed by photopatterning. As supplementary micron scale cylindrical lenses, we fabricated uniform-sized microwires with and 5 and 10 mu m diameters and refractive index similar to 1.3-1.6. The microwires are placed in contact with the specimen to collect the information of the sub-wavelength features of the specimen and transmit them to the far-field with magnification enabling imaging with two-fold resolution improvement. Potential applications of our imaging technique include biological imaging, microfluidics, and nanophotonics applications.

关键词： Super-resolution microscopy nanoscopy microwire

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FPGA Implementation of Spectral Fusing Gabor Domain Optical Coherence microscopy 27

FPGA Implementation of Spectral Fusing Gabor Domain Optical ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Meemon, Panomsak Lenaphet, Yutana Saetiew, Jadsada Suranaree Univ Technol Sch Phys Inst Sci Nakhon Ratchasima 30000 Thailand Suranaree Univ Technol Sch Elect Engn Inst Engn Nakhon Ratchasima 30000 Thailand

ISBN: (纸本)9781510632547

Gabor domain optical coherence microscopy (GD-OCM) is an emerging tomographic imaging technique that overcome the depth of focus (DOF) limit of high numerical objective lens by acquiring multiple cross-section images corresponded with different focal planes of the objective along an axial direction and combining them to form an invariant high resolution cross-section image. We previously reported an alternative processing method of GD-OCM called spectral fusing GD-OCM (SF-GD-OCM). SF-GD-OCM extracts in-focus information directly from acquired spectral interference signals. The advantage of SF-GD-OCM over the original GD-OCM is that the Gabor filtering and fusing can be implemented in an acquisition hardware instead of a host computer. Here, we have investigated the implementation of SF-GD-OCM by using FPGA frame grabber. The experimental setup of the acquisition system as well as the implementation of the spectral Gabor fusion in FPGA device have been developed and verified. Results show that the data processing of SF-GD-OCM in FPGA enabled acquisition device is possible and could lead to improvement in overall imaging speed of GD-OCM.

关键词： Gabor domain optical coherence microscopy Gabor fusion technique FPGA Extended depth of field

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Further developments in addressing depth-variant 3D fluorescence microscopy imaging

Further developments in addressing depth-variant 3D fluoresc...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Ghosh, Sreya Schaefer, Lutz Schuster, Dietwald Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Adv Imaging Methodol Consultat Kitchener ON Canada Regensburg Univ Appl Sci Regensburg Germany

ISBN: (纸本)9780819498625

Three-dimensional (3D) imaging with optical sectioning microscopy uses computational methods to obtain the true fluorescence distribution by ameliorating the effect of defocus, spherical aberration and noise. Inverse algorithms improve image quality at a fraction of the cost of implementing an optical system by accurate modeling of the imaging system. Good inverse imaging algorithms need to be accurate as well as fast. Better understanding of the image formation model is vital to obtain improved restoration through model-based algorithms. Forward imaging models based on a depth-varying point-spread function (DV-PSF) leads to a substantial improvement in the resulting images because it accounts for depth-induced aberrations present in the imaging system. PSFs at every layer can be represented using their principal components. Computation of the forward imaging model using a principal component analysis (PCA) representation of the DV-PSF requires fewer convolutions than a strata based approach investigated in the past. In this paper we present a new algorithm for maximum likelihood image restoration developed based on a PCA representation of the DV-PSF and an accelerated conjugate gradient (CG) iteration scheme. Results obtained with this PCA-CG algorithm from both simulated and experimental fluorescence microscope data are discussed and compared with results obtained from a CG iteration method based on the strata model and linear interpolation of the DV-PSF. The performance of the PCA-CG algorithms is shown to be promising for practical applications.

关键词： Point spread functions Principal component analysis 3D image processing Expectation maximization algorithms 3D modeling image restoration Algorithm development Computer simulations Luminescence Reconstruction algorithms

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processing and improvements in dynamic quantitative phase microscope

Processing and improvements in dynamic quantitative phase mi...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Creath, Katherine Goldstein, Goldie 4D Technol Corp Tucson AZ 85706 USA

ISBN: (纸本)9780819493583

This paper describes recent research and development related to data processing and imaging performance for a dynamic quantitative phase imaging microscope. This microscope provides instantaneous measurements of dynamic motions within and among live cells without labels or contrast agents. It utilizes a pixelated wire grid polarizer mask in front of the camera sensor that enables simultaneous measurement of multiple interference patterns. Optical path difference (OPD) and optical thickness (OT) data are obtained from phase images. Simulated DIC (gradient) and simulated dark field (gradient magnitude) images can be directly obtained from the phase enabling simultaneous capture of brightfield, phase contrast, quantitative phase, DIC and dark field. The OT is further processed to remove background shapes, and enhance topography. This paper presents a number of different processing routines to remove background surface shape enabling quantification of changes in cell position and volume over time. Data from a number of different moving biological organisms and cell cultures are presented.

关键词： phase imaging interference microscopy polarization interferometry cellular imaging cell dynamics optical thickness measurement label-free imaging 4D microscopy quantitative phase microscopy

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Focused Ion Beam techniques for the analysis of biological samples: a revolution in ultramicroscopy?

Focused Ion Beam techniques for the analysis of biological s...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Ballerini, M Milani, M Batani, D Squadrini, F Univ Milan Dipartimento Sci Mat I-20126 Milan Italy

ISBN: (纸本)0819439398

Focused ion Beam (FIB) is a novel technique that allows easy target cell selection, fast operation, high resolution, 3D imaging and sample manipulation during imaging. The FIB technique of microscopy and nanomachining, that is widely spread in semiconductor technology, is reshuffled to open new horizons in the field of life sciences at cellular and subcellular level. FIB is a source of ions which can be precisely oriented and focused on the sample, supported also by an electron source. The resolution can be as low as 15 nm. Two different operation modes are available: "cutting" and "etching" operations of very high precision and at the same time the resulting secondary ions and electrons provide follow up sample imaging. FIB tomography ability has been tested to provide information on cell characterisation, cell division time sequence, view of inner structures and investigation of membrane structural properties. We compare the performances and the advantages of different high-resolution microscopy techniques: Soft X-ray Contact microscopy (SXCM), Focused Ion Beam (FIB) and Transmission Electron microscopy (TEM). These have been used to image Saccharomyces cerevisiae yeast cells, a rather well known sample of great biological interest. TEM is an established technique used as a benchmark for comparison.

关键词： microscopy cell imaging Focused Ion Beam( FIB) cell ultrastructure

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Dual-detection confocal microscopy: high-speed surface profiling without depth scanning 13

Dual-detection confocal microscopy: high-speed surface profi...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XxiiI

作者： Lee, Dong-Ryoung Gweon, Dae-Gab Yoo, Hongki Korea Adv Inst Sci & Technol KAIST Dept Mech Engn Nano Optomechatron Lab 291 Daehak Ro Daejeon 34141 South Korea Hanyang Univ Dept Biomed Engn Biomed Opt & Photomed Lab 222 Wangsimni Ro Seoul 04763 South Korea

ISBN: (纸本)9781628419474

We propose a new method for three-dimensional (3-D) imaging without depth scanning that we refer to as the dual-detection confocal microscopy (DDCM). Compared to conventional confocal microscopy, DDCM utilizes two pinholes of different sizes. DDCM generates two axial response curves which have different stiffness according to the pinhole diameters. The two axial response curves can draw the characteristics curve of the system which shows the relationship between the axial position of the sample and the intensity ratio. Utilizing the characteristic curve, the DDCM reconstructs a 3-D surface profile with a single 2-D scanning. The height of each pixel is calculated by the intensity ratio of the pixel and the intensity ratio curve. Since the height information can be obtained directly from the characteristic curve without depth scanning, a major advantage of DDCM over the conventional confocal microscopy is a speed. The 3-D surface profiling time is dramatically reduced. Furthermore, DDCM can measure 3-D images without the influence of the sample condition since the intensity ratio is independent of the quantum yield and reflectance. We present two types of DDCM, such as a fluorescence microscopy and a reflectance microscopy. In addition, we extend the measurement range axially by varying the pupil function. Here, we demonstrate the working principle of DDCM and the feasibility of the proposed methods.

关键词： Confocal microscopy three-dimensional microscopy high speed imaging

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