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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

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3D reconstruction methods using line-scanning microscopy with a linear sensor

3D reconstruction methods using line-scanning microscopy wit...

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conference on Advanced microscopy Techniques III

作者： Macedo, Milton P. Correia, C. M. B. A. Univ Coimbra Dept Phys Ctr Instrumentat Coimbra Portugal

ISBN: (纸本)9780819496461

Line-scanning microscopy is a technique with ability to deliver images with an higher acquisition rate than confocal microscopy. But it is accomplished at expense of the degradation of resolution for details parallel to sensor if slit detectors are used. With a linear image sensor it is possible to attenuate or even cancel this effect through the use of information stored in each pixel / light distribution across line pixels of the sensor. In spite of its great potential the use of linear image sensors and in particular the development of three-dimensional (3D) reconstruction methods that take into account its specificity is scarce. This led to our motivation to build a laboratory prototype of a bench stage-scanning microscope using a linear image sensor. We aim at improving lateral resolution isotropy but also image visualization and 3D mesh reconstruction using different optical setups particularly illumination modes, e. g., widefield and line-illumination. The versatility of the laboratory prototype namely its software for image acquisition, processing and visualization is important to attain this goal in the sense that it provides excellent means to develop and test algorithms. Several algorithms for 3D reconstruction were developed and are presented and discussed in this paper. Results of the application of these 3D reconstruction methods show the improvements on lateral resolution isotropy and depth discrimination achieved using algorithms integrating sensor geometry or spatial sampling rate. Also it is evidenced the impact of an insufficient spatial sampling rate from 3D mesh reconstructions.

关键词： Three-dimensional microscopy slit-scanning microscopy image reconstruction techniques linear image sensor lateral resolution depth discrimination

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Tunable thin-film optical filters for hyperspectral microscopy

Tunable thin-film optical filters for hyperspectral microsco...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Favreau, Peter F. Rich, Thomas C. Prabhat, Prashant Leavesley, Silas J. Univ S Alabama Biomol Chem engn Mobile AL 36688 USA Univ S Alabama Pharmacol Mobile AL 36688 USA Univ S Alabama Ctr Lung Biol Mobile AL 36688 USA Semrock Inc A Unit Index Mobile AL 36688 USA

ISBN: (纸本)9780819493583

Hyperspectral imaging was originally developed for use in remote sensing applications. More recently, it has been applied to biological imaging systems, such as fluorescence microscopes. The ability to distinguish molecules based on spectral differences has been especially advantageous for identifying fluorophores in highly autofluorescent tissues. A key component of hyperspectral imaging systems is wavelength filtering. Each filtering technology used for hyperspectral imaging has corresponding advantages and disadvantages. Recently, a new optical filtering technology has been developed that uses multi-layered thin-film optical filters that can be rotated, with respect to incident light, to control the center wavelength of the pass-band. Compared to the majority of tunable filter technologies, these filters have superior optical performance including greater than 90% transmission, steep spectral edges and high out-of-band blocking. Hence, tunable thin-film optical filters present optical characteristics that may make them well-suited for many biological spectral imaging applications. An array of tunable thin-film filters was implemented on an inverted fluorescence microscope (TE 2000, Nikon Instruments) to cover the full visible wavelength range. images of a previously published model, GFP-expressing endothelial cells in the lung, were acquired using a charge-coupled device camera (Rolera EM-C2, Q-Imaging). This model sample presents fluorescently-labeled cells in a highly autofluorescent environment. Linear unmixing of hyperspectral images indicates that thin-film tunable filters provide equivalent spectral discrimination to our previous acousto-optic tunable filter-based approach, with increased signal-to-noise characteristics. Hence, tunable multi-layered thin film optical filters may provide greatly improved spectral filtering characteristics and therefore enable wider acceptance of hyperspectral widefield microscopy.

关键词： Hyperspectral imaging spectral imaging microscopy AOTF acousto-optic tunable filter TFTF thin-film tunable filter VersaChrome

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Technique for real-time frontal face image acquisition using stereo system

Technique for real-time frontal face image acquisition using...

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conference on Videometrics, Range Imaging, and Applications xii;and Automated Visual Inspection

作者： Knyaz, Vladimir A. Vizilter, Yuri V. Kudryashov, Yuri I. State Res Inst Aviat Syst Moscow Russia

ISBN: (纸本)9780819496072

Most part of existing systems for face recognition is usually based on two-dimensional images. And the quality of recognition is rather high for frontal images of face. But for other kind of images the quality decreases significantly. It is necessary to compensate for the effect of a change in the posture of a person (the camera angle) for correct operation of such systems. There are methods of transformation of 2D image of the person to the canonical orientation. The efficiency of these methods depends on the accuracy of determination of specific anthropometric points. Problems can arise for cases of partly occlusion of the person's face. Another approach is to have a set of person images for different view angles for the further processing. But a need for storing and processing a large number of two-dimensional images makes this method considerably time-consuming. The proposed technique uses stereo system for fast generation of person face 3D model and obtaining face image in given orientation using this 3D model. Real-time performance is provided by implementing and graph cut methods for face surface 3D reconstruction and applying CUDA software library for parallel calculation.

关键词： face 3D model dynamic programming min cut/max flow CUDA technology

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3D reconstruction methods using line-scanning microscopy with a linear sensor

3D reconstruction methods using line-scanning microscopy wit...

引用

European conference on Biomedical Optics, ECBO 2013

作者： Macedo, Milton P. Correiaa, C.M.B.A. Center of Instrumentation Department of Physics University of Coimbra Coimbra Portugal ISEC Instituto Superior de Engenharia de Coimbra Coimbra Portugal

ISBN: (纸本)9780819496461

Line-scanning microscopy is a technique with ability to deliver images with an higher acquisition rate than confocal microscopy. But it is accomplished at expense of the degradation of resolution for details parallel to sensor if slit detectors are used. With a linear image sensor it is possible to attenuate or even cancel this effect through the use of information stored in each pixel / light distribution across line pixels of the sensor. In spite of its great potential the use of linear image sensors and in particular the development of three-dimensional (3D) reconstruction methods that take into account its specificity is scarce. This led to our motivation to build a laboratory prototype of a bench stage-scanning microscope using a linear image sensor. We aim at improving lateral resolution isotropy but also image visualization and 3D mesh reconstruction using different optical setups particularly illumination modes, e.g., widefield and line-illumination. The versatility of the laboratory prototype namely its software for image acquisition, processing and visualization is important to attain this goal in the sense that it provides excellent means to develop and test algorithms. Several algorithms for 3D reconstruction were developed and are presented and discussed in this paper. Results of the application of these 3D reconstruction methods show the improvements on lateral resolution isotropy and depth discrimination achieved using algorithms integrating sensor geometry or spatial sampling rate. Also it is evidenced the impact of an insufficient spatial sampling rate from 3D mesh reconstructions. © 2013 OSA-SPIE.

关键词： Pixels

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Lens-less holographic microscopy with high resolving power for 4D measurement of microorganism swimming in water

Lens-less holographic microscopy with high resolving power f...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIX

作者： Otani, Mayu Sato, Kunihiro Univ Hyogo Dept Elect Eng & Comp Sci Grad Sch Engn Himeji Hyogo 6712201 Japan

ISBN: (纸本)9780819488701

A lens-less holographic microscopy is developed for observation of high-resolution 4-D (spatio-temporal) images. A complex-amplitude in-line hologram with a large numerical aperture is extracted from a large off-axis hologram by applying one-shot digital holography. An angular spectrum method is developed for fast numerical reconstruction of precise 3-D image from the in-line hologram with a large numerical aperture. 3-D intensity and phase images with no distortion, with a resolution higher than 1 mu m, and with a depth larger than 1mm are recorded and reconstructed by the present holographic microscopy. Possibility is demonstrated to realize 4-D measurement of microorganisms swimming in water.

关键词： lens-less holographic microscopy 4-D measurement microorganism phase measurement

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Three dimensional refractive index imaging with differential interference contrast microscopy

Three dimensional refractive index imaging with differential...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIX

作者： Aung, Htet Buckley, Jared Kostyk, Piotr Rodriguez, Braulio Phelan, Shelley Xu, M. Fairfield Univ Dept Phys 1073 N Benson Rd Fairfield CT 06824 USA

ISBN: (纸本)9780819488701

We report here a new approach based on an extension of the transport of the intensity equation for three dimensional refractive index imaging of a weak phase object from a series of images recorded by a differential interference contrast microscope at different focus (z-stack). Our method is first validated by imaging polystyrene spheres. We then apply this method to monitor in vivo apoptosis of human breast MCF7 epithelial cells. The potential applications are discussed at the end.

关键词： DIC microscopy intensity propagation equation quantitative phase imaging 3D refractive index map

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A Wavelet-Based Bayesian Framework for 3D Object Segmentation in microscopy

A Wavelet-Based Bayesian Framework for 3D Object Segmentatio...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIX

作者： Pan, Kangyu Corrigan, David Hillebrand, Jens Ramaswami, Mani Kokaram, Anil Univ Dublin Trinity Coll Dept Elect & Elect Engn Dublin 2 Ireland Trinity Coll Dublin Inst Neurosci Dublin 2 Ireland

ISBN: (纸本)9780819488701

In confocal microscopy, target objects are labeled with fluorescent markers in the living specimen, and usually appear with irregular brightness in the observed images. Also, due to the existence of out-of-focus objects in the image, the segmentation of 3-D objects in the stack of image slices captured at different depth levels of the specimen is still heavily relied on manual analysis. In this paper, a novel Bayesian model is proposed for segmenting 3-D synaptic objects from given image stack. In order to solve the irregular brightness and out-of-focus problems, the segmentation model employs a likelihood using the luminance-invariant 'wavelet features' of image objects in the dual-tree complex wavelet domain as well as a likelihood based on the vertical intensity profile of the image stack in 3-D. Furthermore, a smoothness 'frame' prior based on the a priori knowledge of the connections of the synapses is introduced to the model for enhancing the connectivity of the synapses. As a result, our model can successfully segment the in-focus target synaptic object from a 3D image stack with irregular brightness.

关键词： image segmentation Wavelets 3D modeling 3D image processing 3D acquisition Feature extraction Confocal microscopy Binary data Discrete wavelet transforms microscopy

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Correction accuracy in image-based adaptive optics for nonlinear microscopy

Correction accuracy in image-based adaptive optics for nonli...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIX

作者： Facomprez, Aurelie Beaurepaire, Emmanuel Debarre, Delphine Ecole Polytech ParisTech Lab Opt & Biosci CNRS F-91128 Palaiseau France INSERM F-91128 Palaiseau France

ISBN: (纸本)9780819488701

We investigate the parameters governing the accuracy of correction in modal sensorless adaptive optics for microscopy. In this paper we focus on the case of two-photon excited fluorescence. Using analytical, numerical and experimental results, we show that using a suitable number of measurements, accurate correction can be achieved for up to 2 rad rms initial aberrations even without optimisation of the correction modes. We demonstrate that this correction can be achieved using low light levels to minimise photobleaching and toxicity, and we provide examples of such optimised correction.

关键词： Adaptive optics aberration correction multiphoton microscopy nonlinear microscopy

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Simultaneous quantitative depth mapping and extended depth of field for 4D microscopy through PSF engineering

Simultaneous quantitative depth mapping and extended depth o...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIX

作者： Zahreddine, Ramzi N. Cormack, Robert H. Cogswell, Carol J. Univ Colorado Dept Elect Comp & Energy Engn Boulder CO 80309 USA

ISBN: (纸本)9780819488701

An extended depth of field (EDF) microscope that allows for quantitative axial positioning has been constructed. Past work has shown that EDF microscopy allows for features in varying planes to appear sharply focused simultaneously, however an inherent consequence of this is that depth information is lost. Here, a specifically engineered phase plate is used to create a point spread function (PSF) that contains both of the necessary attributes for extended depth of field and quantitative depth mapping. A two-camera solution is used to separate and capture the information for individualized post processing. The result is a microscope that can serve as an essential tool for full 3D, real-time imaging.

关键词： 4D microscopy PSF engineering extended depth of field quantitative depth ranging

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Prospective gating for 3D imaging of the beating zebrafish heart in embryonic development studies

Prospective gating for 3D imaging of the beating zebrafish h...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIX

作者： Taylor, J. M. Saunter, C. D. Love, G. D. Girkin, J. M. Univ Durham Dept Phys Ctr Adv Instrumentat Durham DH1 3LE England

ISBN: (纸本)9780819488701

We demonstrate the use of prospective gating from continuously acquired brightfield images of zebrafish embryos to trigger the acquisition of fluorescence images with the heart at a precisely selected position in its cycle. The laser exposure of the sample is reduced by an order of magnitude compared to alternative techniques which acquire many separate fluorescence images for each section before selecting the most appropriate ones to build up a consistent 3D image stack. We present results obtained using our SPIM system including 3D reconstructions of the living, beating heart, acquired using optical gating without the need for any pharmacological or electrophysiological intervention, and discuss possible wider applications of our technique.

关键词： Optical microscopy Selective Plane Illumination Synchronization Fluorescence imaging

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