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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

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Quantitative Characterization of Deformation and Damage in Shale Under Compression Utilizing Digital image Correlation

Quantitative Characterization of Deformation and Damage in S...

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2022 SPE/AAPG/SEG Unconventional Resources Technology conference, URTC 2022

作者： Nath, Fatick Aguirre, Edgardo Aguirre, Gabriel Perez, Jose L. Netro, Cesar Borjas, Flavio Limon, Valeria School of Engineering Texas A&M International University LaredoTX United States

Shale formations are constantly facing challenges ensuring safe drilling and effective fracturing to unlock potential. One of the key influencing factors is accurately characterizing the deformation and damage due to its intrinsic lamination and natural fractures. Moreover, shale formations exhibit fine-scale heterogeneity while the conventional laboratory methods (LVDT, strain gauges, etc.) are unable to distinguish such variation. Besides, methods like acoustic emission (AE), X-ray, computed tomography (CT), and scanning electron microscopy (SEM) are lengthy, costly, and functional with only small test ranges. This study aims to investigate the damage and deformation characteristics in shale samples using three-dimensional digital image correlation (3D-DIC) to overcome such limitations. A set of samples from Wolfcamp, Mancos, and Eagle Ford shale are tested under uniaxial (D×L=2"×2") and diametrical (D×L=2"×1") compression using a precision 100kN Instron electromechanical load frame with a constant displacement of 0.05 mm/min. Specimens had distinct lamination and natural fractures. An ARAMIS 3D-DIC image acquisition system was positioned to monitor the samples at a rate of 2 frames per second in a non-contact manner while conducting the uniaxial and diametrical compression experiment. A black-in-white dot pattern is utilized on the specimen to track the deformation while loading. The ARAMIS 3D-DIC system is used for image processing, visualization, and analysis of the shale damage process with varying load conditions. DIC generated quantitative full-field strain maps with load (tension, compression, and shear) show all sequences in the damage process and strain evolution. The entire damage progress over time of shale samples is split into four stages: initial damage stage, linear elastic, elastic-plastic, and plastic damage stage, which are evaluated by damage variables. In uniaxial and diametrical compression, Eagle Ford shale exhibits central failure due to ten

关键词： Computerized tomography

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Focus stacking and 3D visualization in multispectral microscopy of thick samples

Focus stacking and 3D visualization in multispectral microsc...

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3D image acquisition and Display: Technology, Perception and Applications, 3D 2021 - Part of Imaging and Applied Optics Congress 2021

作者： Alonso, Julia R. Silva, Alejandro Fernández, Ariel Arocena, Miguel Instituto de Física Facultad de Ingeniería Universidad de la República J. Herrera y Reissig 565 Montevideo Uruguay Cátedra de Bioquímica y Biofísica Facultad de Odontología Universidad de la República Montevideo Uruguay

A custom built fluorescence microscope with an electrically tunable lens is used to acquire multi-focus z-stacks of thick biological samples under multispectral illumination. Acquired information allows for 3D visuali... 详细信息

ISBN: (纸本)9781557528209

关键词： Three dimensional computer graphics

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Excitation-scan Mirror Array System Advancements to Hyperspectral Imaging Applications 27

Excitation-scan Mirror Array System Advancements to Hyperspe...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Browning, Craig M. Mayes, Sam A. Deal, Joshua Gunn-Mayes, Samantha Rich, Thomas C. Leavesley, Silas J. Parker, Marina Univ S Alabama Chem & Biomol Engn Mobile AL 36688 USA Univ S Alabama Pharmacol Mobile AL 36688 USA Univ S Alabama Ctr Lung Biol Mobile AL 36688 USA Univ S Alabama Dept Syst Engn Mobile AL 36688 USA

ISBN: (纸本)9781510632547

Hyperspectral imaging (HSI) technology has been applied in a range of fields for target detection and mixture analysis. While its original applications were in remote sensing, modern uses include agriculture, historical document authentications and medicine. HSI has shown great utility in fluorescence microscopy;however, acquisition speeds have been slow due to light losses associated with spectral filtering. We are currently developing a rapid hyperspectral imaging platform for 5-dimensional imaging (RHIP-5D), a confocal imaging system that will allow users to obtain simultaneous measurements of many fluorescent labels. We have previously reported on optical modeling performance of the system. This previous model investigated geometrical capability of designing a multifaceted mirror imaging system as an initial approach to sample light at many wavelengths. The design utilized light-emitting diodes (LEDs) and a multifaceted mirror array to combine light sources into a liquid light guide (LLG). The computational model was constructed using Monte Carlo optical ray software (TracePro, Lambda Research Corp.). Recent results presented here show transmission has increased up to 9% through parametric optimization of each component. Future work will involve system validation using a prototype engineered based on our optimized model. System requirements will be evaluated to determine if potential design changes are needed to improve the system. We will report on-spectral resolution to demonstrate feasibility of the RHIP-5D as a promising solution for overcoming current HSI acquisition speed and sensitivity limitations.

关键词： Systems Design LED Tissue imaging HSI Spectroscopy microscopy Transmission Spectral Fluorescence Molecules Cells

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Wave-optical calibration of a light-field fluorescence microscopy 26

Wave-optical calibration of a light-field fluorescence micro...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVI

作者： Pribosek, J. Steinbrener, J. Baumgart, M. Bereczki, T. Harms, K. Tortschanoff, A. Kenda, A. Carinthian Tech Res Europastr 12 A-9524 Villach Austria Mol Devicess Lagerhausstr 45 A-5071 Wals Austria

ISBN: (纸本)9781510624092

In light-field microscopy, a single point emitter gives rise to a complex diffraction pattern, which varies with the position of the emitter in object space. In order to use deconvolution-based wave-optical reconstruction schemes for light-field imaging systems, established methods rely on theoretical estimation of such diffraction patterns. In this paper we propose a novel method for direct experimental estimation of the light-field point spread function. Our approach relies on a modified reversed micro-Hartmann test to acquire a composite light-field point spread function of several thousand point emitters in the object plane simultaneously. By using fiducial markers and a custom image processing algorithm we separate the contributions of individual point emitters directly in raw light-field images and allow the construction of the forward imaging process without any prior assumption about the optical system required. The constructed forward imaging model can finally be applied in the 3D-deconvolution based wave-optical reconstruction scheme.

关键词： light-field microscopy light-field point spread function wave-optical calibration fluorescence imaging

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Projection multiplexing for enhanced acquisition speed in holographic tomography 26

Projection multiplexing for enhanced acquisition speed in ho...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVI

作者： Kus, Arkadiusz Baczewska, Maria Ziemczonok, Michal Kujawinska, Malgorzata Warsaw Univ Technol Inst Micromech & Photon Sw Boboli 8 St PL-02525 Warsaw Poland

ISBN: (纸本)9781510624092

In holographic tomography (HT), the 3D refractive index distribution within weakly-scattering, phase-only biological object is retrieved. This key property of the technique is one of its most significant strengths compared to labelling-based methods of cell analysis such as fluorescence microscopy. As a consequence, however, it is required to acquire a set of holograms at several viewing directions, which hinders the measurement speed. In this paper we explore the prospect of multiplexing projections in order to decrease the number of scanning positions required for the full measurements. The presented analysis is based on experimental data acquired in a limited-angle holographic tomography system and emulates the performance of a spatial-light-modulator-based system in which multiple projections may be acquired simultaneously by generating a distribution of multiple point sources in the Fourier plane of the condenser lens. For this reason, the increase in acquisition speed strictly depends on the number of multiplexed holograms and results in decreased reconstruction quality. The performance of the system is demonstrated and analyzed with biological objects - human keratinocyte cells.

关键词： Holographic tomography optical diffraction tomography tomographic phase microscopy quantitative phase imaging 3D refractive index hologram multiplexing

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Refractive index properties of the retina accessed by multi-wavelength digital holographic microscopy 26

Refractive index properties of the retina accessed by multi-...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVI

作者： Barroso, Alvaro Ketelhut, Steffi Heiduschka, Peter Nettels-Hackert, Gerburg Schnekenburger, Juergen Kemper, Bjoern Univ Munster Med Fac Biomed Technol Ctr Mendelstr 17 D-48149 Munster Germany Univ Munster Dept Ophtalmol Med Ctr Domagkstr 15 D-48149 Munster Germany

ISBN: (纸本)9781510624092

The refractive index (RI) of the retina and its dispersion are essential parameters in ophthalmologic imaging. However, the spatial RI distribution in retinal tissue is difficult to access. We explored the capabilities of multispectral quantitative phase imaging (QPI) with digital holographic microscopy (DHM) for label-free refractive index characterization of dissected murine retina. The retrieved tissue refractive indices are in agreement with previously reported values for living cells and dissected tissues. Moreover, the detected spatial refractive index distributions correlate with results from complementary conducted OCT investigations. In summary, multispectral DHM is a promising tool for label-free characterization of optical retina properties.

关键词： digital holographic microscopy quantitative phase imaging multi-wavelength hyperspectral imaging retina refractive index

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Enhanced spectral lightfield fusion microscopy via deep computational optics for whole-slide pathology 26

Enhanced spectral lightfield fusion microscopy via deep comp...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVI

作者： Kazemzadeh, Farnoud Li-Chang, Hector Wong, Alexander Univ Waterloo Waterloo ON Canada Waterloo Artificial Intelligence Inst Waterloo ON Canada Royal Victoria Reg Hlth Ctr Barrie ON Canada

ISBN: (纸本)9781510624092

Lensfree on-chip microscopy, which harnesses holography principles to capture interferometric light-field encodings without the need of lenses, is an emerging microscopy modality with widespread interest given the large field-of-view (FOV) compared to lens-based microscopy systems. In particular, there is a growing interest on the development of high-quality lensfree on-chip color microscopy. In this study, we propose a multi-laser spectral lightfield fusion microscopy using deep computational optics for achieving lensfree on-chip color microscopy. We will demonstrate that leveraging deep computational optics can enable imaging resolution beyond the diffraction limit without the use of any complex hardware-based super-resolution techniques, such as aperture scanning. The capabilities of the microscope are examined for whole-slide pathology. The superior imaging resolution of the instrument is demonstrated by imaging of a series of biological specimens demonstrating the true color imaging capability of the instrument while showcasing the large FOV of the instrument.

关键词： on-chip microscopy deep computational optics light-field fusion

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Temporal focusing-based multiphoton excitation fluorescence images with background noise cancellation via Hilbert-Huang transform 26

Temporal focusing-based multiphoton excitation fluorescence ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVI

作者： Hu, Yvonne Yuling Luo, Yuan-Rong Lin, Chun-Yu Chang, Chia-Yuan Chen, Shean-Jen Natl Cheng Kung Univ Dept Photon Tainan Taiwan Natl Cheng Kung Univ Dept Engn Sci Tainan Taiwan Natl Cheng Kung Univ Dept Mech Engn Tainan Taiwan Natl Chiao Tung Univ Inst Imaging & Biomed Photon Coll Photon Tainan Taiwan

ISBN: (纸本)9781510624092

Temporal focusing multiphoton excitation microscopy has wide field-of-view and optical sectioning. By using digital micromirror device, it provides patterned illumination. However, without filling the back aperture of objective lens, the axial confinement is limited to micron-meters, leading the out-of-focus fluorophores excited and image blurred. In this study, Hilbert-Huang transform is proposed to reduce the background noise. The empirical mode decomposition is first applied to disassemble the image into intrinsic mode functions and then reconstruct by Hilbert transform after diminishing background residues. The axial confinement can be enhanced from 2.79 mu m to 0.73 mu m with structure frequency in 1.06 mu m(-1).

关键词： Temporal focusing Digital micromirror device Structured illumination microscopy Hilbert-Huang transform

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Three-dimensional Fluorescence Imaging with an Automated Light-field Microscope 26

Three-dimensional Fluorescence Imaging with an Automated Lig...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVI

作者： Steinbrener, J. Pribosek, J. Baumgart, M. Bereczki, T. Harms, K. Ripper, D. Tortschanoff, A. Kenda, A. CTR Carinthian Tech Res AG Europastr 12 A-9524 Villach Austria IMC Fachhsch Krems GmbH Piaristengasse 1 A-3500 Krems Austria Mol Devices Austria GmbH Lagerhausstr 45 A-5071 Salzburg Austria

ISBN: (纸本)9781510624092

In addition to the two-dimensional intensity distribution in the image plane, light field microscopes capture information about the angle of the incident radiation. This information can be used to extract depth information about the object, calculate all-in-focus images and perform three-dimensional reconstructions from a single exposure. In combination with automated microscopy setups, this makes the technique a promising tool for high-throughput, three-dimensional cell assay evaluation which could substantially improve drug development and screening. To this end, we have developed a novel generalized calibration and three-dimensional reconstruction scheme for a light-field fluorescence microscope setup. The scheme can handle Keplerian and Galilean light field camera configurations added to infinity corrected microscopes configured to be telecentric as well as non-telecentric or hypercentric. The latter provides a significant advantage over the state of the art as it allows for an application specific optimization of lateral and axial resolution, field-of-view, and depth-of-focus. The reconstruction itself is performed iteratively using an expectation maximization algorithm. Super-resolved reconstructions can be achieved by including experimentally measured point-spread-functions. To reduce the required computational power, sparsity and periodicity of the system matrix relating object space to light field space is exploited. This is particularly challenging for the non-telecentric cases, where the voxel size of the reconstructed object space depends on the axial coordinate. We provide details on the experimental setup and the reconstruction algorithm, and present results on the experimental verification of theoretical performance parameters as well as successful reconstructions of fluorescent beads and three-dimensional cell spheroids.

关键词： Light-field microscopy 3D fluorescence imaging Reconstruction algorithms

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Compressed ultrafast transmission electron microscopy: a simulation study 26

Compressed ultrafast transmission electron microscopy: a sim...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVI

作者： Liu, Xianglei Zhang, Shian Yurtsever, Aycan Liang, Jinyang Inst Natl Rech Sci Ctr Energie Mat Telecommun 1650 Blvd Lionel Boulet Varennes PQ J3X 1S2 Canada East China Normal Univ State Key Lab Precis Spect 3663 North Zhongshan Rd Shanghai 200062 Peoples R China

ISBN: (纸本)9781510624092

Bringing ultrafast temporal resolution to transmission electron microscopy (TEM) has historically been challenging. Despite significant recent progress in this direction, it remains difficult to achieve sub-nanosecond temporal resolution with a single electron pulse imaging. To address this limitation, here, we propose a methodology that combines laser-assisted TEM with computational imaging methodologies based on compressed sensing (CS). In this technique, a two-dimensional (2D) transient event [i.e. (x, y) frames that vary in time] is recorded through a CS paradigm. The 2D streak image generated on a camera is used to reconstruct the datacube of the ultrafast event, with two spatial and one temporal dimensions, via a CS-based image reconstruction algorithm. Using numerical simulation, we find that the reconstructed results are in good agreement with the ground truth, which demonstrates the applicability of CS-based computational imaging methodologies to laser-assisted TEM. Our proposed method, complementing the existing ultrafast stroboscopic and nanosecond single-shot techniques, opens up the possibility for single-shot, spatiotemporal imaging of irreversible structural phenomena with sub-nanosecond temporal resolution.

关键词： Transmission electron microscopy ultrafast imaging compressed sensing streak imaging

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