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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

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Point spread function models for digital holographic microscopy

Point spread function models for digital holographic microsc...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Marian, A Depeursinge, C Swiss Fed Inst Technol Inst Biomed Imaging Opt & Engn CH-1015 Lausanne Switzerland

ISBN: (纸本)0819452327

Few models, based on the diffraction theory, are proposed in order to evaluate the point spread function of different microscope objectives used in a digital holographic microscope. Because in holography the phase information is essential, a 3D amplitude point spread function (APSF), modulus and phase, is necessary, in order to properly deconvolute the 3D images obtained. Scalar Debye theory, paraxial approximation and vectorial Debye theory are used to solve the diffraction problem and the theoretical predicted 3D APSFs obtained. with these models are compared.

关键词： point spread function diffraction digital holographic microscopy

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Nano-scale optical imaging technique

Nano-scale optical imaging technique

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Wu, S Tang, YC PEER Ctr Covina CA 91722 USA

ISBN: (纸本)0819452327

The optical resolution limits the observation of fine features with size below diffraction limit, e.g. 500nm. We propose and demonstrate a technique that uses collimated laser scattering to characterize such features. We use a new illumination technique that let us image bio-molecules down to 50nm with optical microscopy. We observed vesicles with size down to 50nm and visualize the flow of such tiny molecules in the live cells. We could also characterize the detailed size and molecular weight of such particles.

关键词： single particle laser scattering microscopy

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Real-time digital design for an optical coherence tomography acquisition and processing system

Real-time digital design for an optical coherence tomography...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Ralston, TS Mayen, JA Marks, D Boppart, SA Univ Illinois Beckman Inst Adv Sci & Technol Urbana IL 61801 USA

ISBN: (纸本)0819452327

We present a real-time, multi-dimensional, digital, optical coherence tomography (OCT) acquisition and imaging system. The system consists of conventional OCT optics, a rapid scanning optical delay (RSOD) line to support fast data acquisition rates, and a high-speed A/D converter for sampling the interference waveforms. A 1M-gate Virtex-II field programmable gate array (FPGA) is designed to perform digital down conversion. This is analogous to demodulating and low-pass filtering the continuous time signal. The system creates in-phase and quadrature-phase components using a tunable quadrature mixer. Multistage polyphase finite impulse response (FIR) filtering and down sampling is used to remove unneeded high frequencies. A floating-point digital signal processor (DSP) computes the magnitude and phase shifts. The data is read by a host machine and displayed on screen at real-time rates commensurate with the data acquisition rate. This system offers flexible acquisition and processing parameters for a wide range of multi-dimensional optical microscopy techniques.

关键词： digital signal processing optical coherence tomography Doppler spectroscopic real-time

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A phase-shifting DIC technique for measuring 3D phase objects: Experimental verification

A phase-shifting DIC technique for measuring 3D phase object...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： King, SV Cogswell, CJ Univ Colorado Dept Elect & Comp Engn Boulder CO 80309 USA

ISBN: (纸本)0819452327

Experimental verification of our previously proposed linear phase imaging technique for differential interference contrast microscopy (DIC) microscopy is presented. This technique first applies phase-shifting methods to DIC to acquire linear phase gradient images in two orthogonal directions. A special Fourier integration algorithm is then applied to the combined phase gradient images to create a single linear phase image in which intensity is proportional to phase. This overcomes the limitations of traditional DIC, which cannot accurately measure the phase (i.e. refractive index or thickness) of embedded 3D phase objects. The linear phase imaging technique is implemented using a standard DIC microscope altered to allow controlled phase shifting, a low noise CCD camera, and post-processing in Matlab. The results presented confirm the linear proportionality of intensity to phase in these images.

关键词： DIC phase phase gradient phase imaging spiral phase interference microscopy

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Agile optical confocal microscopy instrument architectures for high flexibility imaging

Agile optical confocal microscopy instrument architectures f...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Riza, NA Bokhari, A Nuonics Inc Orlando FL 32817 USA

ISBN: (纸本)0819452327

Ideally, a no-moving parts fast and agile scanning confocal microscope system is required that can produce true realtime 3-D scans with precision and repeatability. In this paper, such agile optical confocal microsopy designs are proposed that enable high speed precise non-invasive 3-D imaging. These compact confocal microscopes can provide real-time pin-point focussed imaging to enable confocal slices in-vivo, thus greatly reducing motion artifacts. These microscopes can be modified into interferometric microscopes for phase contrast imaging. The proposed microscopes can also greatly improve confocal fluorescence imaging as needed for cancer detection. An ultracompact confocal probe tip connected to a single ultra-thin fiber is another design option allowing flexibility for usage in tight cavities.

关键词： confocal microscopy fiber-optics instrument 3-D imaging

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Confocal microscopy with variable-focal length microlenses

Confocal microscopy with variable-focal length microlenses

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Mac Raighne, A Yang, LS Dunbar, LA McCabe, EM Scharf, T Trinity Coll Dublin Dept Phys Dublin 2 Ireland

ISBN: (纸本)0819452327

Confocal microscopy has a unique optical sectioning property, which allows three-dimensional images at different depths. Use of a microlens array is a potential alternative to the Nipkow disk for parallel imaging with high throughput in real-time confocal microscopy. The use of variable-focal-length microlenses can provide a way to axially scan the foci electronically avoiding the inflexible mechanical movement of the lens or the sample. Here we demonstrate a combination of a variable-focal-length microlens array and a fibre optic bundle as a way to create a high throughput aperture array that would be potentially applied as confocal imaging in vivo biological specimens. The incident side of the microlens array was determined by examining the focus distribution in the axial direction. The variation of the focal length obtained by changing the voltage and corresponding focus intensity were measured through a conventional microscope. Meanwhile, the fibre bundle was characterized by coupling with either coherent or incoherent light source. We use the fibre bundle as both a multiple aperture and an image-carrying element and combine it with a microlens array to built up a confocal system.

关键词： confocal microscopy endoscopy microlenses fibre bundle

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Phase imaging digital holography for biological microscopy

Phase imaging digital holography for biological microscopy

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Kim, MK Parshall, D Univ S Florida Dept Phys Tampa FL 33620 USA

ISBN: (纸本)0819452327

The digital holographic microscopy is being developed, to offer a number of significant advantages and capabilities in phase imaging. The direct availability of both the amplitude and phase information offers a range of versatile processing techniques that can be applied to the complex field data, including the phase imaging which is particularly straightforward in digital holography. One of the techniques we are developing addresses the problem of 2-pi phase discontinuity in the phase image, where most of the conventional phase unwrapping algorithms require subjective intervention for multi-lambda discontinuity. In the present system, we generate two phase maps by digital holography using two different wavelengths. Numerical superposition of the two phase maps results in a new phase map whose effective wavelength is inversely proportional to the difference of the two wavelengths. The axial range can in principle be arbitrarily large compared to the wavelength, while maintaining axial resolution to a fraction of wavelength. We apply the phase imaging digital holography to a number of systems, including the imaging of thin films and biological cells. Preliminary results from these experiments are presented and future development and applications are discussed.

关键词： phase imaging digital holography phase unwrapping microscopy multi-wavelength imaging

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Optimization of spinning disk confocal microscopy: Synchronization with the ultra-sensitive EMCCD

Optimization of spinning disk confocal microscopy: Synchroni...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Chong, FK Coates, CG Denvir, DJ McHale, N Thornbury, K Hollywood, M Andor Technol Ltd Belfast BT12 7AL Antrim North Ireland

ISBN: (纸本)0819452327

The advent of Electron Multiplying Charge Coupled Device (EMCCD) technology and it's ability to overcome previous hurdles in low-light fluorescence microscopy, such as phototoxicity to live cells, photobleaching of fluorophores and exposure time restrictions, has resulted in a significant resurgence of interest in use of confocal spinning disk techniques for live cell microscopy. Here provide an understanding of, and technical solutions to, the issues of synchronization that have previously marred the coupling of fast CCD camera technology to confocal spinning disk arrangements. We examine the challenges arising from both old and new models of the Nipkow spinning disk confocal unit and suggest solutions throughout based on a sound comprehension of both (a) relative scan/exposure times;(b) relative orientation of the coupled devices;(c) optimisation of EMCCD clocking parameters.

关键词： CCD EMCCD Nipkow confocal spinning disk confocal microscopy live cell microscopy electron multiplying CCD single photon sensitivity

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Bright field segmentation tomography (BFST) for use as surface identification in stereomicroscopy

Bright field segmentation tomography (BFST) for use as surfa...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Thiesse, J Namati, E de Ryk, J Hoffman, E McLennan, G Univ Iowa Iowa City IA 52242 USA

ISBN: (纸本)0819452327

Stereomicroscopy is an important method for use in image acquisition because it provides a 3D image of an object when other microscopic techniques can only provide the image in 2D. One challenge with this type of imaging is determining the top surface of a sample that has otherwise indistinguishable surface and planar characteristics. We have developed a system that creates a form of oblique illumination and in conjunction with image processing, the top surface can be viewed. The use of BFST consists of the Leica MZ16A stereomicroscope with a unique attached lighting source, and is part of the Large image Microscope Array (LIMA) [1]. The lighting source consists of eight light emitting diodes (LED's) that are separated by 45-degree angles. Each LED in this system illuminates with a 20-degree viewing angle once per cycle with a shadow over the rest of the sample. Subsequently, eight segmented images are taken per cycle. After the images are captured they are stacked through image addition to achieve the full field of view, and the surface is then easily identified. image processing techniques, such as skeletonization, can be used for further enhancement and measurement. With the use of BFST, advances can be made in detecting surface features from metals to tissue samples, such as in the analytical assessment of pulmonary emphysema using mean linear intercept.

关键词： stereomicroscopy illumination surface identification image processing

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Design and performance evaluation of reflection confocal microscopy using acousto-optical deflector and slit detector

Design and performance evaluation of reflection confocal mic...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Lee, SW Kang, DK Yoo, HK Gweon, DG Lee, SW Kim, KS Korea Adv Inst Sci & Technol Dept Mech Engn Taejon South Korea

ISBN: (纸本)0819452327

We describe the design and the implementation of reflection confocal scanning microscopy (CSM) using an acousto-optical deflector (AOD) for the fast horizontal scan and a galvanometer mirror (GM) for the slow vertical scan. In the beam scanning system it is important to maintain the lateral and the axial performance during scanning operation. We propose a simple method to design a scanning system using the finite ray tracing and the diffraction theory. We define a cost function which contains the effect of aberrations on the performance of microscopy. We construct the designed system and evaluate its performance. The OSLO simulation shows that the performances of CSM are not changed with deflection angle. So we conclude that the beam scanning system is properly designed. In addition, we propose an image formation method and show images obtained with the system.

关键词： confocal microscopy beam scanning system acousto-optical deflector slit detector finite ray tracing relay optics image formation

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