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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

共 561 条记录，以下是481-490 订阅

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Three-dimensional imaging with a quadrature tomographic microscope

Three-dimensional imaging with a quadrature tomographic micr...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Stott, JJ Bennett, RE Warner, CM DiMarzio, CA Northeastern Univ Ctr Subsurface Sensing & Imaging Syst Boston MA 02115 USA

ISBN: (纸本)0819439398

The quadrature tomographic microscope combines conventional microscopy with interferometric quadrature detection techniques to yield a simultaneous measurement of both the amplitude and phase of the electric field. This is of tremendous utility in imaging nearly-transparent objects since changes in phase can be directly attributed to changes in the object's index of refraction while the small amplitude changes provide additional structural information. In this paper we will discuss the progress we have made towards reconstructing three dimensional images from the measured two-dimensional complex electric field. BS applying an artificial curvature to the measured field and back-propagating the Gaussian beams, we are able to digitally manipulate the imaging kernel and image different focal planes.

关键词： microscopy optical quadrature tomography quantitative biology

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Programmable array microsope employing two ferroelectric liquid crystal spatial light modulators

Programmable array microsope employing two ferroelectric liq...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Taylor, CM McCabe, EM Univ Dublin Trinity Coll Dept Phys Dublin 2 Ireland

ISBN: (纸本)0819439398

We present a programmable array microscope (PAM) which uses a pair of ferroelectric liquid crystal spatial light modulators (FLC SLMs). The system is similar to a confocal imaging system called a direct-view microscope (DVM), in which scanned aperture arrays are used to obtain real-time confocal images. The PAM, unlike the DVM, allows arrays to be scanned electronically rather than mechanically. In our system, one SLM is placed in the source plane of a conventional microscope system;the other is placed in the detector plane. Confocal aperture arrays are displayed and scanned synchronously on the SLMs and confocal imaging results. The resolution is improved when compared to a similar previously presented system which employed a single SLR I. We present axial resolution measurements for a variety of array dimensions and investigate the use of aperture correlation techniques to improve the light throughput in the devices.

关键词： programmable array microscopy ferroelectric liquid crystal spatial light modulator confocal microscope direct-view microscopy

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Focused Ion Beam techniques for the analysis of biological samples: a revolution in ultramicroscopy?

Focused Ion Beam techniques for the analysis of biological s...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Ballerini, M Milani, M Batani, D Squadrini, F Univ Milan Dipartimento Sci Mat I-20126 Milan Italy

ISBN: (纸本)0819439398

Focused ion Beam (FIB) is a novel technique that allows easy target cell selection, fast operation, high resolution, 3D imaging and sample manipulation during imaging. The FIB technique of microscopy and nanomachining, that is widely spread in semiconductor technology, is reshuffled to open new horizons in the field of life sciences at cellular and subcellular level. FIB is a source of ions which can be precisely oriented and focused on the sample, supported also by an electron source. The resolution can be as low as 15 nm. Two different operation modes are available: "cutting" and "etching" operations of very high precision and at the same time the resulting secondary ions and electrons provide follow up sample imaging. FIB tomography ability has been tested to provide information on cell characterisation, cell division time sequence, view of inner structures and investigation of membrane structural properties. We compare the performances and the advantages of different high-resolution microscopy techniques: Soft X-ray Contact microscopy (SXCM), Focused Ion Beam (FIB) and Transmission Electron microscopy (TEM). These have been used to image Saccharomyces cerevisiae yeast cells, a rather well known sample of great biological interest. TEM is an established technique used as a benchmark for comparison.

关键词： microscopy cell imaging Focused Ion Beam( FIB) cell ultrastructure

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Proceeding of SPIE - The International Society for Optical Engineering

Proceeding of SPIE - The International Society for Optical E...

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Three-dimensional and multidimensional microscopy: image Asquisition and Processin VIII

The proceedings contains 18 papers from SPIE 2001 conference on Three dimensional and multidimensional microscopy: image acquisition and processing VIII. The topics discussed include: inverse scattering;fiber optic co... 详细信息

关键词： Optical microscopy

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One-step calibration of specimen induced focal shifts and spherical aberration for quantitative 3D microscopy:: Approach and first results

One-step calibration of specimen induced focal shifts and sp...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Thomann, D Stüssi, E Danuser, G Swiss Fed Inst Technol Biomech Lab CH-8952 Schlieren Switzerland

ISBN: (纸本)0819439398

With the advent of laser scanning confocal and multiphoton microscopy, 3D life tissue characterization has been rendered possible. This involves the restoration of thick section images (in the depth range of 100 microns) of biological samples. In contrast to thin samples new effects become important when imaging thick samples: Because of changes of the refractive index across the specimen or the embedding medium, strong (> 1 micron) focal shifts and spherical aberration occur and scattering effects get more prominent. For tissue mapping it is essential to correct for such aberrations and distortion effects. In this paper. Re propose a calibration framework, which allows us to determine thick section focal shifts and spherical aberrations in a one-step-procedure, Gradients in the focal shift induce a scaling in the Z-direction of the observed sample. A second effect arises with depth-dependent spherical aberration. We model our microscope as a linear-shift-NON-invariant system (LSNI) where multiple depth classes are assigned distinct point spread functions (PSFs). We measure these two effects in a 3D sample of randomly distributed fluorescent focal check beads. The beads are embedded in gelatine, a medium with high resemblance to real biological tissue. The PSF is approximated by a mathematical parametric model. While estimating the parameters of the PSF with object-constrained deconvolution, we track depth dependent changes of the observed bead diameter. This allows us to determine the gradient of the focal shift across a thick section. By numerical integration along the optical axis we obtain the focal shift values as a function of sample depth. In the end, these values will be applied as a correction to compensate depth distortions in the tissue images.

关键词： confocal microscopy calibration spherical aberration focal shifts deconvolution

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Quantification of trabecular bone structure from three-dimensional μMR images

Quantification of trabecular bone structure from three-dimen...

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International conference on image processing (ICIP 2001)

作者： Accardo, A Candido, G Toffanin, R Vittur, F Jellús, V Univ Trieste DEEI Trieste Italy

ISBN: (纸本)0780367251

Magnetic resonance imaging (MRI) has recently been applied to the study of osteoporosis. Accurate acquisition techniques and image analysis protocols for the determination of trabecular bone structure are yet to be defined. In this study two different approaches of three-dimensional acquisition and reconstruction were exploited to derive the structural parameters of trabecular bone explants. In particular, MR microscopy (muMRI) was used to obtain conventional (FT) and projection reconstruction (PR) spin-echo images (41x41x41 mum(3)/voxel). Segmentation of the high resolution images into bone and bone marrow was obtained by using a suitable threshold. The main bone structural parameters were then derived by using a 3D version of the directed secant algorithm applied to a series of contiguous sections. The efficiency of the two methods in the assessment of trabecular bone architecture is discussed.

关键词： Magnetic resonance imaging

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Three dimensional simulations of SEM imaging and charging

Three dimensional simulations of SEM imaging and charging

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conference on Metrology, Inspection, and Process Control for Microlithography XV

作者： Grella, L Lorusso, G Niemi, T Chuang, TC Adler, DL KLA Tencor Corp San Jose CA 95134 USA

ISBN: (纸本)0819440302

SEM based CD control and wafer inspection has an increasingly active role in the semiconductor industry. Current design rules require a CD control with a precision in the nanometer range. In order to achieve this precision, a complete model of the image formation mechanism is desirable. For this reason we present a three-dimensional simulation of scanning electron microscope (SEM) images. The simulations include Monte Carlo electron scattering, charging in the substrate and electron ray-tracing in the column. We investigate some specific cases in CD-SEM metrology: We will describe the effect of scan orientation relative to the orientation of the imaged feature on the apparent beam width (ABW), the effect of magnification on contact imaging, and the effect of residue in resist trenches. Our results, regarding these examples, clearly indicate that a fully three-dimensional numerical simulation is needed to obtain an understanding of image formation and resolution limiting factors.

关键词： Monte Carlo methods Scanning electron microscopy 3D image processing 3D modeling image acquisition 3D metrology Computer simulations image resolution Metrology Scattering

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Applying DIP techniques to microscopic biological images

Applying DIP techniques to microscopic biological images

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conference on Nonlinear image processing and Pattern Analysis xii

作者： Araújo, AA Faria, BM Silva, MAR Reis, HJ Univ Fed Minas Gerais Dept Ciencias Computacao Nucl Proc Digital Imagens BR-31270010 Belo Horizonte MG Brazil

ISBN: (纸本)0819439827

This work reports and illustrates the application of enhancement techniques to animal nervous system images from a Laser Scanning Confocal Microscope. images obtained from this equipment are used to help researchers on localizing several organelles and proteins. Different image components of the same tissue sample can be acquired varying the confocal microscope laser bean wavelength. Due to non-ideal acquisition, numerous images contain artifacts, poor distribution of gray levels and unsystematic contrast gradient. Several techniques have been implemented in order to enhance the images, including noise and artifacts reduction, contrast expansion and enhancements on organelles borders, such as emboss and 3D-visualization. A methodology to accurately solve the frequent contrast gradient problem has been implemented. The approach is based on blurring filter, histogram equalization and arithmetic operations. image coloring is another issue. Each of the acquired components must be merged into one single image with its respective color. The final phase of the work consisted of gathering all implemented techniques to elaborate an application that enclosed facilities to automatically open files from confocal file format (.pic format), apply the developed methodologies to enhance the images, build the multicomponent artificial color image and save the results in common formats. This application must deal with large amounts of images easily, providing facilities to batch processing and image indexing and labeling.

关键词： confocal microscopy image processing image editor image enhancement

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Quantification of trabecular bone structure from three-dimensional /spl mu/MR images

Quantification of trabecular bone structure from three-dimen...

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IEEE International conference on image processing

作者： A. Accardo G. Candido R. Toffanin F. Vittur V. Jellus DEEI DEEI University of Trieste Italy Dept. BBCM University of Trieste Italy IMS Slovak Academy of Sciences Bratislava Slovakia

Magnetic resonance imaging (MRI) has recently been applied to the study of osteoporosis. Accurate acquisition techniques and image analysis protocols for the determination of trabecular bone structure are yet to be defined. Two different approaches for three-dimensional acquisition and reconstruction are exploited to derive the structural parameters of trabecular bone explants. In particular, MR microscopy (/spl mu/MRI) is used to obtain conventional (FT) and projection reconstruction (PR) spin-echo images (41/spl times/41/spl times/41 /spl mu/m/sup 3//voxel). Segmentation of the high-resolution images into bone and bone marrow is obtained by using a suitable threshold. The main bone structural parameters are then derived by using a 3D version of the directed secant algorithm applied to a series of contiguous sections. The efficiency of the two methods in the assessment of trabecular bone architecture is discussed.

关键词： Cancellous bone Magnetic resonance imaging image analysis image resolution Osteoporosis image reconstruction microscopy image segmentation Histograms Protocols

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4D image processing in microscopy by combined methods

4D image processing in microscopy by combined methods

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conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Kriete, A Image Proc Lab Inst Anat & Cell Biol D-35385 Giessen Germany

ISBN: (纸本)081943535X

In time-lapse microscopy image quality is limited by instrumental and specimen characteristics. Moreover, an enormous amount of data may be accumulated. This challenges not only the development of new tools for image processing, but typically forces methods to be executed in combination. To remedy essential problems, an image processing pipeline is employed which features image restoration, image compression, visualization and analysis. To control the flow of information within this pipeline and to optimize the parameter settings like compression rates or number of iterations, the information content is evaluated on the basis of the ratio of signal and noise.

关键词： time-lapse microscopy 4D image processing compression image restoration visualization and analysis

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