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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

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3,3′-thiodipropanol as a refractive index matching mounting medium for fluorescence microscopy 27

3,3′-thiodipropanol as a refractive index matching mounting...

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Alata Tejedo, Milvia Iris Martinez Cervantes, Juan Carlos Piazza, Valeria Ctr Invest Opt AC Loma Bosque 115 Guanajuato Mexico

ISBN: (纸本)9781510632547

In this work, several tests of the performance of 3,3'-thiodipropanol (TDP) as a mounting medium for fluorescence microscopy of biological samples were performed. Besides optical properties like the dispersion curve of TDP and the effect of the embedding medium on the fluorescence of commonly used dyes, the interaction with biological specimens, including the labeling of filamentous actin with fluorescent phalloidins, was tested: TDP showed to represent an interesting alternative to commercial mounting media. By mixing TDP with 2,2'-thiodiethanol (TDE), it was possible not only to fine tune the refractive index of the resulting solution, but to preserve the compatibility with fluorescent phalloidins.

关键词： Mounting media fluorescence microscopy filamentous actin

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Point spread functions with extended depth of focus

Point spread functions with extended depth of focus

引用

conference on 3-dimensional and multidimensional microscopy: image acquisition and processing IX

作者： Wilson, T Neil, MAA Massoumian, F Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

ISBN: (纸本)0819443603

The use of an annular pupil plane filter may be used to increase the depth of focus of an objective lens without significant deterioration of the lateral resolution. However this approach is very inefficient since most of the illumination light is blocked by the annular filter. We describe a method which uses a diffractive optical element to increase significantly the depth of focus but with dramatically increased light efficiency.

关键词： Axicons Point spread functions Optical components Binary data Optical filters Objectives Beam shaping Diffractive optical elements image filtering Roads

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Agile optical confocal microscopy instrument architectures for high flexibility imaging

Agile optical confocal microscopy instrument architectures f...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Riza, NA Bokhari, A Nuonics Inc Orlando FL 32817 USA

ISBN: (纸本)0819452327

Ideally, a no-moving parts fast and agile scanning confocal microscope system is required that can produce true realtime 3-D scans with precision and repeatability. In this paper, such agile optical confocal microsopy designs are proposed that enable high speed precise non-invasive 3-D imaging. These compact confocal microscopes can provide real-time pin-point focussed imaging to enable confocal slices in-vivo, thus greatly reducing motion artifacts. These microscopes can be modified into interferometric microscopes for phase contrast imaging. The proposed microscopes can also greatly improve confocal fluorescence imaging as needed for cancer detection. An ultracompact confocal probe tip connected to a single ultra-thin fiber is another design option allowing flexibility for usage in tight cavities.

关键词： confocal microscopy fiber-optics instrument 3-D imaging

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Deformable mirror based remote focusing for fast three-dimensional microscopy 13

Deformable mirror based remote focusing for fast three-dimen...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XxiiI

作者： Zurauskas, Mantas Frade, Maria Booth, Martin Univ Oxford Ctr Neural Circuits & Behav Mansfield Rd Oxford OX1 3SR England Univ Oxford Dept Engn Sci Parks Rd Oxford OX1 3PJ England

ISBN: (纸本)9781628419474

We present a deformable mirror based remote focusing method for three-dimensional imaging in high-resolution microscopy systems. The method relies on predefined mirror mode arrays that are obtained during initial mirror training step with a low complexity wavefront-sensing module. The imaging plane can be refocused over distances over a hundred times greater than the original depth of field of the objective lens along the optical axis at millisecond rates. We will demonstrate the combination of the remote focusing method with spatiotemporally focused two-photon excitation applied to three-dimensional imaging of biological samples.

关键词： Remote focusing adaptive optics three dimensional microscopy two photon microscopy

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Efficient calibration algorithm and calibration pattern for correcting distortions for three-dimensional image acquisition systems for microscopic applications

Efficient calibration algorithm and calibration pattern for ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Walecki, Wojtek J. Szondy, Fanny Sunrise Opt LLC Sunrise FL 33351 USA

ISBN: (纸本)9780819470362

We present novel method for fully automated detection of calibration features. It reduces computation effort when compared to traditional corner, edge, and conic detection algorithms. Our method employs generalized method based on properties of prospective projection invariants [1], but is also applicable for the optical systems suffering high order, non-spherical distortions. We also show an example when in presence of significant barrel distortion procedure developed for ideal pinhole camera system outlined in [1] does not converge to Unique solution. We suggest new modified algorithm which produces unique solution.

关键词： machine vision camera calibration image processing

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microscopy environment for quantitative spatial and temporal analysis of multicellular interactions

Microscopy environment for quantitative spatial and temporal...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing IX

作者： Sudar, D Parvin, B Callahan, DE Schwarz, RI Knowles, DW de Solorzano, CO Barcellos-Hoff, MH Lawrence Berkeley Lab Div Life Sci Berkeley CA 94720 USA

ISBN: (纸本)0819443603

Quantitative analysis of spatial and temporal concurrent responses of multiple markers in 3-dimensional cell cultures is hampered by the routine mode of sequential image acquisition, measurement and analysis of specific targets. A system was developed for detailed analysis of multi-dimensional, time-sequence responses and in order to relate features in novel and meaningful ways that will further our understanding of basic biology. Optical sectioning of the 3-dimensional structures is achieved with structured light illumination using the Wilson grating as described by Lanni. The automated microscopy system can image multicellular structures and track dynamic events, and is equipped for simultaneous/ sequential imaging of multiple fluorescent markers. Computer-controlled perfusion of external stimuli into the culture system allows (i) real-time observations of multiple cellular responses and (ii) automatic and intelligent adjustment of experimental parameters. This creates a feedback loop in real-time that directs desired responses in a given experiment. On-line image analysis routines provide cell-by-cell measurement results through segmentation and feature extraction (i.e. intensity, localization, etc.), and quantitation of meta-features such as dynamic responses of cells or correlations between different cells. Off-line image and data analysis is used to derive models of the processes involved, which will deepen the understanding of the basic biology.

关键词： live-cell microscopy 3-dimensional imaging real-time feedback image analysis image bioinformatics

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Tracking fluorescent spots in biological video microscopy

Tracking fluorescent spots in biological video microscopy

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Genovesio, A Olivo-Marin, JC Inst Pasteur Quantitat Image Anal Grp F-75724 Paris France

ISBN: (纸本)0819447641

The analysis of cell and pathogen movement and motility is a major topic in cell biology for which computerized methods are most needed. This study proposes a method to detect and track multiple moving biological objects in image sequences acquired through fluorescence video microscopy. The method enables the analysis of video microscopy image sequences in order to obtain reliable quantitative data such as number, position, speed and movement phases. The method consists of three stages. A stage of detection is performed through a multi-scale analysis of images using an undecimated wavelet transform. The next stage is the prediction of the state of each detected spot in the next frame using a Kalman filter and an adapted model. Then comes a stage of data association which constructs the tracks and refines the filters. Once all moving objects have been assigned with unique spatio-temporal paths, trajectories are analyzed in terms of different parameters relevant to the motility analysis of biological objects.

关键词： image processing tracking wavelets Kalman filter data associations biological video microscopy fluorescent spots

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Near-field optical and atomic force constraints for super-resolution 3D deconvolution in far-field optical microscopy

Near-field optical and atomic force constraints for super-re...

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conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Axelrod, N Radko, A Ben-Yosef, N Khatchatouriants, A Treinin, M Lewis, A Hebrew Univ Jerusalem Dept Appl Phys IL-91904 Jerusalem Israel

ISBN: (纸本)081943535X

We demonstrate that near-field optical and atomic force microscopy data can be used for super-resolution three-dimensional (3-D) image restoration in optical sectioning fluorescence microscopy. A crucial feature in this approach is the full integration of such data sets with digital far-field images. The scanned probe data is used to provide modalities for boundary constraints which define surface optical information and spatial domains of optical alterations in a sample with a spatial precision that has been unachievable in the past. A restoration algorithm that can use such a complex of data for 3-D image deconvolution is presented. It uses a Tikhonov-Miller regularization scheme and allows for the imposition of different types of constraints to obtain super-resolution deconvolved images. Performance was tested by using simulated 3-D imaging. An example is given of the restoration of a 3-D wide field optical image of a biological specimen in the presence of atomic force constraints.

关键词： image restoration deconvolution algorithm constraints probe microscopy NSOM super-resolution

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Quasi-confocal fluorescence sectioning with dynamic speckle illumination

Quasi-confocal fluorescence sectioning with dynamic speckle ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing xiiI

作者： Ventalon, Cathie Mertz, Jerome Boston Univ Dept Biomed Engn 44 Cummington St Boston MA 02215 USA

ISBN: (纸本)0819461326

We present a new fluorescence microscopy technique that provides depth discrimination in thick tissue. The technique relies on a simple modification to a conventional wide-field microscope, and consists in illuminating the sample with a sequence of speckle patterns and displaying the RMS or the variance of the resultant sequence of fluorescent images. We demonstrate quasi-confocal optical sectioning with an axial resolution of 5 microns FWHM. The lateral resolution is identical to the widefield microscope, namely 0.6 micron FWHM. images of a mouse brain are compared with standard wide-field images, demonstrating an efficient quasi-sectioning capacity in thick tissue throughout approximatively 100 microns depth. This is achieved because of the high contrast maintained by speckle in a scattering media.

关键词： fluorescence microscopy optical sectioning 3D imaging linear microscopy confocal microscopy speckle structured light illumination thick tissue imaging

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Fast scanning confocal microscopy with a rotary mirror array

Fast scanning confocal microscopy with a rotary mirror array

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Chen, NG Zhu, Q Univ Connecticut Dept Elect & Comp Engn Storrs CT 06269 USA

ISBN: (纸本)0819447641

We have developed a novel confocal microscopy system, which can perform both axial and lateral scanning at an ultra high speed. A rotary mirror array (RMA) consists of 36 small mirrors deployed on the surface of a round plate with a small tilting angle and a discrete rotational symmetry. When the RMA rotates at a constant speed, the focal point is scanned inside a sample periodically and linearly. We have demonstrated a scanning rate of 2400 lines per second, which can be readily improved to more than 20000 lines per second. Our scheme can also be used in a fast scanning multi-photon microscope.

关键词： rotary mirror array fast scanning confocal microscopy optical coherence microscopy

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