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Generation and imaging with the third harmonic

Generation and imaging with the third harmonic

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conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Brakenhoff, GJ Squier, J Univ Amsterdam BioCentrum Dept Mol Cyt NL-1098 SM Amsterdam Netherlands

ISBN: (纸本)081943535X

In this paper we study the spatial distribution and wavefront characteristics of third harmonic generation in relation to some material and interface conditions over the focal region of the fundamental beam. We investigate, mostly from an experimental point of view, the implications the physics of the THG generation process has in situations where THG may be employed for -3D- imaging. Due to the non-linear character of the THG generation process it is inherently suitable for this application. For the first time images of the distribution of the THG radiation, as the interface is moved through focus, are shown. Experiments on closely spaced interfaces or bilayers confirm unambiguously the correctness of the vector model for THG generation (Ward et al. 1969) in uniform media. In view of these and other data the image formation, especially for biological objects, with THG radiation will be discussed.

关键词： THG third harmonic microscopy imaging

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A study for accelerating the speed of all-in-focus image processing 27

A study for accelerating the speed of all-in-focus image pro...

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Wang, Lihui Tabata, Satoshi Takeuchi, Hirotoshi Ishikawa, Masatoshi Guangdong Acad Sci Guangdong Inst Semicond Ind Technol 363 Changxing Rd Guangzhou 510650 Peoples R China Univ Tokyo Grad Sch Informat Sci & Technol Bunkyo Ku 7-3-1 Hongo Tokyo 1138656 Japan

ISBN: (纸本)9781510632547

To capture an all-in-focus and 3D depth image, shape from focus method is widely used. The phase accuracy of the image candidates should be processed and adjusted beforehand Phase only correction method was employed in this work and to accelerate the processing speed, the image processing by Fast Fourier Transform (FFT) was optimized Meanwhile, the processing task was assigned in several parallel threads so that the performance would be improved. The method was used on a variable focus imaging system, and, as a result, the processing speed was improved to around 2.5-fps.

关键词： all-in-focus shape from focus phase-only correction method 3D imaging variable focus system

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High-resolution volumetric imaging using hybrid image of Fourier light-field microscopy and wide-field microscopy 29

High-resolution volumetric imaging using hybrid image of Fou...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIX

作者： Liu, Wenhao Jia, Shu Georgia Inst Technol Wallace H Coulter Dept Biomed Engn Atlanta GA 30332 USA Emory Univ Atlanta GA 30332 USA

ISBN: (纸本)9781510648043;9781510648036

We have developed wFLFM to further enhance the resolution by taking the advantage of both wide-field image and FLFM. We proposed the principle of wFLFM and verified the approach both numerically using simulated rings, and experimentally using phantom samples on the wFLFM system we constructed as a prototype. The principle of wFLFM requires only additional wild-field image at the aperture plane, making it readily compatible with various extended depth of focus wide-field modules, and the validating results showed a 2-3x enhancement in lateral resolution without cost in axial information, thereby advancing wFLFM as a promising high-resolution 3D imaging method.

关键词： Light-field microscopy Fourier light-field microscopy high resolution volumetric imaging

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3D microscopy with a double-helix point spread function

3D microscopy with a double-helix point spread function

引用

conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVI

作者： Pavani, Sri Rama Prasanna Piestun, Rafael Univ Colorado Dept Elect & Comp Engn Boulder CO 80309 USA

ISBN: (纸本)9780819474308

Double-helix point spread function (DH-PSF) is an engineered three-dimensional (3D) PSF specifically designed for 3D position estimation and imaging. It exhibits two lobes that rotate continuously around the optical axis with propagation. An information theoretical analysis shows that the DH-PSF carries higher and more uniform Fisher Information than the standard PSF through the 3D volume of interest. Experiments with DH-PSF demonstrate nanometer scale position localization accuracies in all three dimensions. Further, a variety of microscopy techniques such as bright-field, dark-field, and fluorescence can be directly transformed into their DH-PSF counterparts by placing an appropriate phase mask in the imaging path. The flexibility and performance make the DH-PSF attractive for 3D position localization in both photon-limited and photon-unlimited regimes.

关键词： Three-dimensional microscopy Point spread function engineering Bright-field Dark-field Fluorescence Computational Optical sensing and imaging

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Fuzzy Logic Components for Iterative Deconvolution Systems

Fuzzy Logic Components for Iterative Deconvolution Systems

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Northan, Brian M. True North Image Proc Guilderland NY 12084 USA

ISBN: (纸本)9780819493583

Deconvolution systems rely heavily on expert knowledge and would benefit from approaches that capture this expert knowledge. Fuzzy logic is an approach that is used to capture expert knowledge rules and produce outputs that range in degree. This paper describes a fuzzy-deconvolution-system that integrates traditional Richardson-Lucy deconvolution with fuzzy components. The system is intended for restoration of 3D widefield images taken under conditions of refractive index mismatch. The system uses a fuzzy rule set for calculating sample refractive index, a fuzzy median filter for inter-iteration noise reduction, and a fuzzy rule set for stopping criteria.

关键词： Deconvolution Fuzzy Logic Spherical Aberration

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High-speed focal modulation microscopy using acousto-optical modulators for visualization of thick biological specimens

High-speed focal modulation microscopy using acousto-optical...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Chong, Shau Poh Gao, Guangjun Sheppard, Colin J. R. Chen, Nanguang Natl Univ Singapore Div Bioengn Singapore 117576 Singapore

ISBN: (纸本)9780819484413

In recent development of fluorescence microscopy, the out-of-focus fluorescence background that arises when imaging deep inside biological tissues is critical in determining the image quality and penetration depth. Focal modulation microscopy (FMM) is an advanced fluorescence technique that can provide high subcellular resolution when imaging thick specimens mainly by preserving the signal-to-background ratio.

关键词： Fluorescence microscopy multiple scattering penetration depth

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3D Effects in DIC images of Extended Objects

3D Effects in DIC Images of Extended Objects

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVI

作者： Sierra, Heidy DiMarzio, Charles A. Brooks, Dana H. Northeastern Univ Dept Elect & Comp Engn Boston MA 02115 USA

ISBN: (纸本)9780819474308

Phase microscopy has been widely used to acquire information about unstained transparent biological objects. These objects are well visualized by techniques such as Differential Interference Contrast (DIC) microscopy, but standard DIC systems don't provide quantitative phase information. Quantitative analysis is limited by the nonlinear relation between the intensity image and the object phase. In this work we combine nonlinearity concepts of the image formation process to model 3D DIC images, specifically the point spread function (PSF) formulation;capturing behavior of measured images along the optical axis that are not obtained when using other models. We verify our model by comparing simulated images to real measured images. This model is designed so that the reconstruction of the 3D properties of imaged specimens can be done in a simple form.

关键词： Differential Interference Contrast (DIC) thick objects coherent microscopy image formation theory

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A Wavelet-Based Bayesian Framework for 3D Object Segmentation in microscopy

A Wavelet-Based Bayesian Framework for 3D Object Segmentatio...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIX

作者： Pan, Kangyu Corrigan, David Hillebrand, Jens Ramaswami, Mani Kokaram, Anil Univ Dublin Trinity Coll Dept Elect & Elect Engn Dublin 2 Ireland Trinity Coll Dublin Inst Neurosci Dublin 2 Ireland

ISBN: (纸本)9780819488701

In confocal microscopy, target objects are labeled with fluorescent markers in the living specimen, and usually appear with irregular brightness in the observed images. Also, due to the existence of out-of-focus objects in the image, the segmentation of 3-D objects in the stack of image slices captured at different depth levels of the specimen is still heavily relied on manual analysis. In this paper, a novel Bayesian model is proposed for segmenting 3-D synaptic objects from given image stack. In order to solve the irregular brightness and out-of-focus problems, the segmentation model employs a likelihood using the luminance-invariant 'wavelet features' of image objects in the dual-tree complex wavelet domain as well as a likelihood based on the vertical intensity profile of the image stack in 3-D. Furthermore, a smoothness 'frame' prior based on the a priori knowledge of the connections of the synapses is introduced to the model for enhancing the connectivity of the synapses. As a result, our model can successfully segment the in-focus target synaptic object from a 3D image stack with irregular brightness.

关键词： image segmentation Wavelets 3D modeling 3D image processing 3D acquisition Feature extraction Confocal microscopy Binary data Discrete wavelet transforms microscopy

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Optical design of a snapshot high-sampling image Mapping Spectrometer (IMS) for hyperspectral microscopy

Optical design of a snapshot high-sampling Image Mapping Spe...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Gao, Liang S. Kester, Robert T. Tkaczyk, Tomasz S. Rice Univ Dept Bioengn Houston TX 77005 USA

ISBN: (纸本)9780819479662

A snapshot high-sampling image Mapping Spectrometer (IMS) is developed for hyperspectral microscopy, measuring datacube of dimensions 285x285x60 (x,y,lambda). microscopy IMS is designed to work at the Nyquist sampling limit to realize high resolution imaging. The spatial resolution is similar to 0.45 mu m with FOV similar to 130 mu m. The spectral working range is 500-700nm with similar to 8.3nm average spectral resolution. Preliminary tests have been implemented on its imaging performances.

关键词： Hyperspectral microscopy image Mapping Spectrometer

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3D deconvolution of adaptive-optics corrected retinal images

3D deconvolution of adaptive-optics corrected retinal images

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing xiiI

作者： Chenegros, G. Mugnier, L. M. Lacombe, F. Glanc, M. Off Natl Etud & Rech Aerosp Dept Opt BP 72 F-92322 Chatillon France Mauna Kea Technol F-75010 Paris France Lab Etudes Spatiales Instrument Astrophys Paris France

ISBN: (纸本)0819461326

We report on a deconvolution method developed in a Bayesian framework for adaptive-optics corrected images of the human retina. The method takes into account the three-dimensional nature of the imaging process;it incorporates a positivity constraint and a regularization metric in order to avoid uncontrolled noise amplification. This regularization metric is designed to simultaneously smooth noise out and preserve edges, while staying convex in order to keep the solution unique. We demonstrate the effectiveness of the method, and in particular of the edge-preserving regularization, on realistic simulated data.

关键词： image restoration deconvolution three-dimensional imaging adaptive optics retinal imaging microscopy

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