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检索条件"任意字段=Conference on 3-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XII"

共 561 条记录，以下是81-90 订阅

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A compact polarization converter to observe molecular orientation

A compact polarization converter to observe molecular orient...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Hashimoto, Mamoru Yoshiki, Keisuke Kanamaru, Ryosuke Hashimoto, Nobuyuki Araki, Tsutomu Osaka Univ Grad Sch Engn Sci 1-3 Machikaneyama Toyonaka Osaka 5608531 Japan Citizen Watch Co Ltd MHT R&D Dept Tokorozawa Saitama 3598511 Japan

ISBN: (纸本)9780819465566

We developed a compact polarization-converter using two liquid-crystal spatial-light-modulators with eight electrodes. The converter converted a linearly polarized beam to two orthogonal linearly polarized beams and a radially polarized beam, and the direction of the electric filed at the focal point were controlled three-dimensionally. We constructed a second-harmonic-generation microscope using the polarization-converter to observe three-dimensional molecular orientation and demonstrated the detectability of molecular orientation.

关键词： molecular orientation nonlinear microscopy second-harmonics-generation pupil filtering radially polarized beam

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Multiple beam interference confocal microscopy - a tool for morphological investigation of living cells and tissues

Multiple beam interference confocal microscopy - a tool for ...

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conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Joshi, NV Medina, H Univ Los Andes Fac Med Dept Physiol Merida 5101 Venezuela

ISBN: (纸本)081943535X

Multiple beam interference system is used in conjunction with a conventional scanning confocal microscope to examine the morphology and construction of 3-D images of Histolytic Ameba and parasite Candida Albicans. The present combination permits to adjoin advantages of both systems, namely the vertical high contrast and optical sectioning. The interference pattern obtained from a multiple internal reflection of a sample, sandwiched between the glass plate and the cover plate, was focussed on an objective of a scanning confocal microscope. According to optical path differences, morphological details were revealed. The combined features, namely improved resolution in z axis, originated from the interference pattern and the optical sectioning of the confocal scanning system, enhance the resolution and contrast dramatically. These features permitted to obtain unprecedented images of Histolytic Ameba and parasite Candida Albicans. Because of the improved contrast, several details like double wall structure of candida, internal structure of ameba are clearly visible.

关键词： Confocal microscopy Microscopes Digital image correlation Glasses Tissues Refractive index 3D image processing microscopy Optical scanning systems Phase contrast

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Investigation of 3D microscopy using intensity diffraction tomography

Investigation of 3D microscopy using intensity diffraction t...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing xiiI

作者： Anastasio, Mark A. Huang, Yin Gbur, Greg Carney, P. Scott IIT Dept Biomed Engn Chicago IL 60616 USA Univ North Carolina Charlotte Dept Phys & Opt Sci Charlotte NC USA Univ Illinois Dept Elect & Comp Engn Urbana IL USA

ISBN: (纸本)0819461326

Diffraction tomography (DT) is an established imaging technique for reconstructing the complex-valued refractive index distribution of a weakly scattering 3D sample. Due to experimental difficulties associated with the direct measurement of the phase of an optical wavefield, the effectiveness of DT for optical imaging applications has been limited. A theory of intensity diffraction tomography (I-DT) has been proposed to circumvent this phase retrieval problem. In this work, we review the features of I-DT reconstruction theory that are relevant to optical microscopy. Computer-simulation studies are conducted to investigate the performance of reconstruction algorithms for a proposed I-DT microscope. The effects of data noise are assessed, and statistically optimal reconstruction strategies that employ multiple detector planes are proposed.

关键词： diffraction tomography three-dimensional microscopy tomographic image reconstruction

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Single image sectioning using spatial frequency domain imaging 24

Single image sectioning using spatial frequency domain imagi...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXIV

作者： Hoffman, Zachary R. DiMarzio, Charles A. Northeastern Univ 360 Huntington Ave Boston MA 02115 USA Draper Lab 555 Technol Sq Cambridge MA 02139 USA

ISBN: (数字)9781510605824

ISBN: (纸本)9781510605817;9781510605824

Structured illumination microscopy (SIM) works well to produce depth information by removing undesired light from out of focus planes within a specimen. However, it generally requires multiple images in order to produce sectioning. We show that, under the right conditions, we can leverage Spatial Frequency Domain Imaging (SFDI) to produce both sectioning and relative depth information about a specimen using only a single image. After processing, we find that the axial resolution is comparable to that of traditional 3 phase SIM. With this technique, we are able to produce real-time videos in-vivo at 21 frames per second. Additionally, no moving parts are needed to produce sectioning at depth.

关键词： Structured Illumination optical sectioning super-resolution incoherent SFDI

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Three dimensional imaging using phase retrieval with two focus planes 13

Three dimensional imaging using phase retrieval with two foc...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XxiiI

作者： Ilovitsh, Tali Ilovitsh, Asaf Weiss, Aryeh Meir, Rinat Zalevsky, Zeev Bar Ilan Univ Fac Engn IL-5290002 Ramat Gan Israel Bar Ilan Univ Bar Ilan Inst Nanotechnol & Adv Mat IL-5290002 Ramat Gan Israel

ISBN: (纸本)9781628419474

This work presents a technique for a full 3D imaging of biological samples tagged with gold-nanoparticles (GNPs) using only two images, rather than many images per volume as is currently needed for 3D optical sectioning microscopy. The proposed approach is based on the Gerchberg-Saxton (GS) phase retrieval algorithm. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. In addition, since the method requires the capturing of two images only, it can be suitable for 3D live cell imaging. The proposed concept is presented and validated both on simulated data as well as experimentally.

关键词： Three-dimensional microscopy Cellular imaging gold nanoparticle Phase retrieval

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Source coherence and optical sectioning strength in direct-view microscopy

Source coherence and optical sectioning strength in direct-v...

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conference on 3-dimensional and multidimensional microscopy: image acquisition and processing IX

作者： Taylor, CM Yang, L McCabe, EM Univ Dublin Trinity Coll Dept Phys Dublin 2 Ireland

ISBN: (纸本)0819443603

The direct-view microscope is a confocal microscope which allows faster image acquisition rates than typical confocal scanning optical microscopes through the use of a pinhole array rather than the usual single pinhole. We present a theoretical investigation of the effects of source coherence on optical sectioning in direct-view microscopy. As a first step we present an equation which describes the optical sectioning strength of a coherent source brightfield DVM employing an infinite pinhole array. By simulation of both this and the finite array equation which show the existence of certain 'principal' sidelobes which are likely to represent the most problematic artifact of coherent source imaging. By further analysis of the infinite Arran, equation, we arrive at an expression which describes the defocus positions where the principal sidelobes occur. Finally, we move on to show how rectangular arrays are predicted, by the infinite array equation, to outperform square arrays and we show examples of this.

关键词： optical sectioning source coherence Real-Time imaging direct-niew microscopy Confocal microscopy

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Phase mask optimization for 3D parallax EDF microscopy

Phase mask optimization for 3D parallax EDF Microscopy

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Beckers, Ingeborg E. Gierlak, Michael Hoeppel, Robert Landskron, Juergen Beuth Univ Appl Sci Berlin Dept Appl Phys Med Engn D-13347 Berlin Germany

ISBN: (纸本)9780819498625

Extended depth-of-field (EDF) microscopy is a well-investigated and very simple method to obtain projection images with an extended depth of focus. Despite its advantages of being a real-time method applicable to any microscopic mode with high lateral resolution that can be simply realized by extending a commercial microscope, the lack of z-correlation is still a problem. In this work we present a combined technique of EDF and stereomicroscopy. By cross-correlation depth information is obtained. Finally, 3D images are reconstructed for best phase masks and simulation results are evaluated experimentally.

关键词： 3D imaging real time imaging extended depth of field microscopy EDF space light modulation SLM stereoscopy parallax EDFP

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Dynamic structured illumination microscopy: focused imaging and optical sectioning for moving objects

Dynamic structured illumination microscopy: focused imaging ...

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conference on Three-dimensional and multidimensional microscopy - image acquisition and processing xiiI

作者： Krzewina, Leo G. Kim, Myung K. Univ S Florida Dept Phys Tampa FL 33620 USA

ISBN: (纸本)0819461326

Structured illumination microscopy (SIM) is a valuable tool for three-dimensional microscopy and has numerous applications in bioscience. Its success has been limited to static objects, though, as three sequential image acquisitions are required per final processed, focused image. To overcome this problem we have developed a multicolored grid which when used in tandem with a color camera is capable of performing SIM with just a single exposure. images and movies demonstrating optical sectioning of three-dimensional objects are presented, and results of applying color SIM for wide-field focused imaging are compared to those of SIM. From computer modeling and analytical calculations a theoretical estimate of the maximum observable object velocity in both the lateral and axial directions is available, implying that the new method will be capable of imaging a variety of live objects. Sample images of the technique applied to lens paper and a pigeon feather are included to show both advantages and disadvantages of CSIM.

关键词： three-dimensional microscopy microscopy optical sectioning structured illumination

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Automated 3D dendritic spine detection and analysis from two-photon microscopy

Automated 3D dendritic spine detection and analysis from two...

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conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Koh, IYY Lindquist, WB SUNY Stony Brook Dept Appl Math & Stat Stony Brook NY 11794 USA

ISBN: (纸本)0819439398

The functional significance of dendritic spines and their plasticity to a wide spectrum of developmental and pathological conditions has led to extensive studies based on spine morphology. The advances in image acquisition techniques and the associated generation of large 3D data sets of optical micrographs have not been accompanied by comparable advances in data analysis techniques. We present an automated 3D spine detection and quantification procedure suitable for images obtained by laser scanning microscopy. The image is first processed by deconvolution and the dendritic phase consisting of the neuronal cytoplasm is extracted by segmentation. Spines are detected as geometrical protrusions relative to the dendritic backbone, ks very thin necks may not be imaged, some spine 'heads' may be detached from the dendrite and are detected as detached components. These detected heads are merged with spine 'bases' where appropriate. Morphological characterizations on spine length, volume, density and shape classifications are obtained. For time-lapse data, images are registered and individual spines are traced through the image sequence. Successful comparison results on spine lengths and densities with manual analysis are obtained. This method is highly automatic and allows detailed and objective quantification of the structure and dynamics of dendritic spines, which can be important predictors for the function of neural networks.

关键词： image processing dendrite spine two-photon medial axis laser scanning microscopy

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Variable-focus microlenses as a potential technology for endoscopy

Variable-focus microlenses as a potential technology for end...

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conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Smith, PJ McCabe, EM Taylor, CM Selviah, DR Day, SE Commander, LG Univ Dublin Trinity Coll Dept Phys Dublin 2 Ireland

ISBN: (纸本)081943535X

Using a new device which contains an array of microlenses whose focal lengths can be electrically varied, we have been able to control the input from one microlens to a single mode fibre using an applied voltage. For such a microlens array many closely-spaced focal spots can be generated in parallel, and electrically switched to address, potentially, an array of receiver fibres. We show how the particular switching characteristics of the device, whereby the lenses switch from diverging to converging, serves in turn to disperse light and to focus it into the fibre.

关键词： microlenses endoscopy direct view microscopy

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