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检索条件"任意字段=Conference on Adaptive Optics and Wavefront Control for Biological Systems IX"

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adaptive optics and wavefront control for biological systems ix

Adaptive Optics and Wavefront Control for Biological Systems...

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adaptive optics and wavefront control for biological systems ix 2023

ISBN: (纸本)9781510658813

The proceedings contain 15 papers. The topics discussed include: performance metrics and active temperature control of spatial light modulators;3D imaging in two photon microscopy using deformable lenses;light sheet fluorescence microscopy with sensor-based adaptive optics and a confocal guide star;large FoV correction using adaptive lenses and deconvolution;simultaneous control of 3D volume speckle field and 3D holography through biological tissue;enhancing speckle statistics for imaging inside scattering tissue;enhancing speckle statistics for imaging inside scattering tissue;imaging through specialty optical fibers;neural-network-based multimode fiber imaging and position sensing under thermal perturbations;beam shaping with multimode fiber based on real-valued intensity transmission matrix for endoscopic applications;and rapid fluorescent wavefront shaping using incoherent power iterations.

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Large FoV correction using adaptive Lenses and Deconvolution 9

Large FoV correction using Adaptive Lenses and Deconvolution

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conference on adaptive optics and wavefront control for biological systems ix

作者： Furieri, Tommaso Calisesi, Gianmaria Ancora, Daniele Bassi, Andrea Bonora, Stefano Univ Padua Dept Informat Engn Via Trasea 7 I-35131 Padua Italy Politecn Milan Dipartimento Fis Piazza Leonardo da Vinci 32 I-20133 Milan Italy CNR IFN Via Trasea 7 I-35131 Padua Italy

In this presentation we show a method capable of measuring and correcting field dependent aberration in a microscope setup without a dedicated wavefront sensor using a pupil conjugated deformable lenses in combination... 详细信息

ISBN: (纸本)9781510658813;9781510658820

关键词： Deconvolution Lenses Microscopy adaptive optics Microscopes Optical microscopy Point spread functions Sensors Signal to noise ratio wavefronts

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Navigating stable light fields through moving complex scattering media 9

Navigating stable light fields through moving complex scatte...

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conference on adaptive optics and wavefront control for biological systems ix

作者： Phillips, David B. Mididoddi, Chaitanya Sharp, Christina Horsley, Simon Univ Exeter Dept Phys & Astron Exeter EX4 4QL Devon England

ISBN: (纸本)9781510658813;9781510658820

The scattering of light distorts imaging through opaque materials. A technique called wavefront shaping enables complicated scattering effects to be characterised and subsequently cancelled out - as long as the turbid sample stays static. Here we study light control through dynamic complex media. We concentrate on a particular class of time-varying scattering media: partially moving scattering systems, which possess time-varying local regions embedded at unknown locations throughout a static matrix. We present both theoretical and experimental results demonstrating the navigation of light around moving parts of a scattering medium. These techniques may find use in future deep-tissue imaging and atmospheric optical communication scenarios.

关键词： Light scattering Tissues Scattering media wavefronts biological samples control systems Matrices Optical fibers Reflection Tissue optics

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Light Sheet Fluorescence Microscopy with Sensor-based adaptive optics and a confocal guide star 9

Light Sheet Fluorescence Microscopy with Sensor-based Adapti...

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conference on adaptive optics and wavefront control for biological systems ix

作者： Liu, Bingxi Liu, Yang Duffy, Carly Lauderdale, James D. Kner, Peter Univ Georgia Sch Elect & Comp Engn Athens GA 30602 USA Univ Georgia Dept Cellular Biol Athens GA 30602 USA

ISBN: (纸本)9781510658813;9781510658820

Light Sheet Fluorescence Microscopy is an excellent tool for imaging larger and thicker model organisms such as zebrafish larvae. Samples can be imaged with high spatial and temporal resolution over large fields of view without photodamage. But Light Sheet Microscopy still suffers from optical aberrations due to the thickness of the samples and the high resolution. We have previously demonstrated imaging of the zebrafish central nervous system and correction of aberrations using sensorless adaptive optics. Sensorless AO is slow because many images are required to achieve a correction. In this work, we demonstrate measuring the wavefront using a Shack-Hartmann wavefront Sensor and a confocal spot as the guide star. This approach does not require special sample preparation or an additional laser. Here we demonstrate our approach by imaging fluorescent beads and inducing wavefront errors with the deformable mirror.

关键词： adaptive optics Light sheet microscopy Neuroimaging wavefront Sensing

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Simultaneous control of 3D volume speckle field and 3D holography through biological tissue 9

Simultaneous control of 3D volume speckle field and 3D holog...

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conference on adaptive optics and wavefront control for biological systems ix

作者： Kumar, Amit Thakur, Sarvesh Biswas, S. K. Indian Inst Sci Educ & Res Mohali Dept Phys Sci BionanoPhoton Lab Knowledge CitySector 81Manauli PO SAS Nagar 140306 India

ISBN: (纸本)9781510658813;9781510658820

Formation of complex light structures and 3-dimensional complex holography through turbid media is a challenging task due to random variation in refractive index, scattering of light, and formation of speckle noise field. We present a binary phase based FLC-SLM in binary mode assisted GA algorithm where the R-squared optimization function is introduced for wavefront shaping. An advanced system design has been developed from scratch with FLC-SLM (ferroelectric liquid crystal spatial light modulator) and dual camera to construct multiple complex structures simultaneously in 3D volume independently without any co-linearity. The formation of multiple complex structures simultaneously in 3D volume has been demonstrated with our prototype system using fresh chicken tissue of thickness 612 mu m as well as a 220 grit ground glass diffuser.

关键词： adaptive optics 3D holography wavefront shaping biological tissue

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Photonic lantern wavefront reconstruction in a multi-wavefront sensor single-conjugate adaptive optics system 9

Photonic lantern wavefront reconstruction in a multi-wavefro...

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conference on adaptive optics systems ix

作者： Sengupta, Aditya B. Diaz, Jordan Gerard, Benjamin L. Jensen-Clem, Rebecca Dillon, Daren DeMartino, Matthew Bundy, Kevin Cetre, Sylvain Chambouleyron, Vincent Univ Calif Santa Cruz Dept Astron & Astrophys Santa Cruz CA 95064 USA Lawrence Livermore Natl Lab Livermore CA 94550 USA Wakea Consulting Durham England

ISBN: (纸本)9781510675186;9781510675179

Exoplanet direct imaging using adaptive optics (AO) is often limited by non-common path aberrations (NCPAs) and aberrations that are invisible to traditional pupil-plane wavefront sensors (WFSs). This can be remedied by focal-plane (FP) WFSs that characterize aberrations directly from a final science image. Photonic lanterns (PLs) can act as low-order FPWFSs with the ability to direct some light to downstream science instruments. Using a PL on the SEAL (Santa Cruz Extreme AO Laboratory) high-contrast imaging testbed, we demonstrate (1) linear ranges and (2) closed-loop control. Additionally, we simulate the use of the PL in a multi-wavefront sensor AO system, in which multiple WFSs feed back to the same common-path deformable mirror. Building on previous multi-WFS AO demonstrations on SEAL, we simulate a modulated pyramid WFS to sense aberrations of high spatial order and large amplitude, and the PL to sense low order aberrations including NCPAs. We assess adaptive optics performance in this setting using three different PL wavefront reconstruction algorithms. We also provide a new method to experimentally identify the propagation matrix of a PL, making advanced model-based algorithms practical. This work demonstrates the role of photonic technologies and multi-stage wavefront sensing in the context of extreme AO and high contrast imaging.

关键词： Astrophotonics photonic lantern wavefront sensing wavefront control adaptive optics multi-stage adaptive optics

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Deep self-supervised learning for computational adaptive optics in widefield microscopy 9

Deep self-supervised learning for computational adaptive opt...

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conference on adaptive optics and wavefront control for biological systems ix

作者： Kang, Iksung Zhang, Qinrong Ji, Na Univ Calif Berkeley Dept Mol & Cell Biol Berkeley CA 94720 USA Univ Calif Berkeley Dept Phys Berkeley CA 94720 USA Univ Calif Berkeley Helen Wills Neurosci Inst Berkeley CA 94720 USA Lawrence Berkeley Natl Lab Mol Biophys & Integrated Bioimaging Div Berkeley CA 94720 USA

ISBN: (纸本)9781510658813;9781510658820

Optical microscopy with adaptive optics (AO) allows high-resolution noninvasive imaging of subcellular structures in living organisms. As alternatives to hardware-based AO methods, supervised deep-learning approaches have recently been developed to estimate optical aberrations. However, these approaches are often limited in their generalizability due to discrepancies between training and imaging settings. Moreover, a corrective device is still required to compensate for aberrations in order to obtain high-resolution images. Here we describe a deep self-supervised learning approach, called CoCoA, for simultaneous aberration estimation and structural information recovery from a single 3D image stack acquired by widefield microscopy. The approach utilizes coordinate-based neural representations to represent highly complex structures. We experimentally validated our approach with direct-wavefront-sensing-based AO in the same samples and showed the approach is applicable to in vivo mouse brain imaging.

关键词： Machine learning adaptive optics Widefield microscopy

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Performance metrics and active temperature control of spatial light modulators 9

Performance metrics and active temperature control of spatia...

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conference on adaptive optics and wavefront control for biological systems ix

作者： Wolenski, Connor Linnenberger, Anna Meadowlark Opt 5964 Iris Pkwy Frederick CO 80504 USA

ISBN: (纸本)9781510658813;9781510658820

Liquid crystal on silicon (LCoS) spatial light modulators (SLMs) are versatile scientific tools relevant to an increasingly wide variety of research and technological applications including digital holography, wavefront correction, optical tweezing, and non-mechanical beam steering to name a few. Since SLMs are used in a multitude of different ways, some aspects of device performance (e.g., response time) are crucial to certain applications while being irrelevant to others. In this work we couple our standard SLMs with a thermo-electric cooler, allowing for tunability of the device operating temperature from 0 degrees - 75 degrees C. We show that there is an inherent tradeoff between the liquid crystal response time and the phase stability of an SLM, and that the operating temperature offers a means of controlling this tradeoff. Furthermore, this paper aims to provide the reader with a brief but thorough explanation of SLM operating principles and device structure, defines the performance metrics of the SLM, and provides a methodology for measuring the specifications. By allowing control over the SLM operating temperature and detailing how temperature affects device functionality, SLM users are afforded greater experimental flexibility and will be better able to tailor the performance of their device for the given project or application at hand.

关键词： Spatial light modulator SLM liquid crystal LCoS optoelectronics electro-optic devices polarization adaptive optics holography

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Large Laser Written Liquid Crystal Devices for Spherical Aberration Correction 10

Large Laser Written Liquid Crystal Devices for Spherical Abe...

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conference on adaptive optics and wavefront control for biological systems X

作者： Xu, Alec Nourshargh, Camron Salter, Patrick S. Elston, Steve J. Morris, Stephen M. Booth, Martin J. Univ Oxford Dept Engn Sci Parks Rd Oxford OX1 3PJ England

ISBN: (纸本)9781510669628;9781510669611

We present a new liquid crystal-based device for correction of spherical aberration, which is commonly observed in microscopy and related methods. The device is significantly larger than previous direct laser written aberration correctors, measuring 1 mm across. The device operates in transmission mode for easy integration into the optical path and is capable of continuous greyscale tuning of up to a total amplitude of 2 pi rad. This device could present a cost effective and simpler to use alternative to traditional wavefront modulation technologies used in adaptive optics.

关键词： adaptive optics liquid crystals Zernike modes direct laser writing

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Revisiting optical transfer function-based focal plane wavefront sensors for correcting NCPAs 9

Revisiting optical transfer function-based focal plane wavef...

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conference on adaptive optics systems ix

作者： Martinez, P. Dharmadhikari, R. Spang, A. Univ Cote Azur Observ Cote Azur Lab Lagrange CNRS Nice France Indian Inst Astrophys II Block Kormangala Bangalore 560034 Karnataka India Univ Calcutta Dept Appl Opt & Photon Kolkata India

ISBN: (纸本)9781510675186;9781510675179

We examine several focal plane wavefront sensing (FPWFS) approaches from a theoretical perspective, specifically comparing their fundamental origins and similarities. We consider various techniques, including the differential optical transfer function wavefront sensor, the self-coherent camera wavefront sensor, the fast-modulated self-coherent camera wavefront sensor, and other variants, as well as pair-wise probing. They are evaluated within a unified framework based on precise optical transfer function analysis and their implementation on the high-contrast imaging SPEED test bed. These concepts are highly similar in essence, even serving as mere extensions of one another. Indeed, we emphasise that these FPWFS can be seen as different expressions or embodiment of the same underlying principles.

关键词： Exoplanets wavefront control active optics extremely large telescopes instrumentation

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