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A novel biomimetic olfactory cell-based biosensor with DNA-directed site-specific immobilization of cells on a microelectrode array

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sensorS AND ACTUATORS B-CHEMICAL 2015年 217卷 186-192页

作者： Du, Liping Zou, Ling Wang, Qin Zhao, Luhang Huang, Liquan Wang, Ping Wu, Chunsheng Zhejiang Univ Dept Biomed Engn Minist Educ Biosensor Natl Special LabKey Lab Biomed Engn Hangzhou 310027 Peoples R China Zhejiang Univ Sch Med Dept Biochem & Genet Hangzhou 310058 Zhejiang Peoples R China Monell Chem Senses Ctr Philadelphia PA 19104 USA Chinese Acad Sci State Key Lab Transducer Technol Shanghai 200050 Peoples R China

The coupling of cells with transducers is a key step to develop cell-based biosensors in order to meet the specific performance requirements for biomedical applications. This study describes a novel biomimetic olfactory cell-based biosensor by the employment of a DNA-directed site-specific cell immobilization method, which can achieve controllable and high-efficient coupling between olfactory cells and the microelectrode array (MEA) chip. A pair of complementary thiol-modified single-stranded DNA (ssDNA) was designed and synthesized. One of them was covalently attached to the plasma membrane of olfactory cells that were isolated from rats. The other one was used as ssDNA probes and immobilized on the gold surface of microelectrodes on MEA chip. Based on the mechanism of complementary ssDNA hybridization, olfactory cells were site-specifically immobilized on the surface of microelectrodes. The distribution of olfactory cells on the surface of MEA chip was controlled by the pattern of ssDNA probes on the sensor surface. The fluorescent staining results indicate that olfactory cells were site-specifically coupled with the microelectrode array on the surface of a MEA chip, which formed a desirable olfactory cell array. In addition, extracellular recordings from the coupled olfactory cells demonstrate that microelectrodes can efficiently monitor the membrane potential changes originated from olfactory cells in response to odorant stimulations. This biomimetic olfactory cell-based biosensor can not only be used for the detection of odorant molecules, but also be applied for the research on the olfactory signal transduction mechanisms. This DNA-directed cell immobilization method provides a novel approach for controllable cell immobilization on solid support and consequently, contributes to the development of cell micro arrays for cell-based biosensors. (C) 2014 Elsevier B.V. All rights reserved.

关键词： Biosensor Olfactory cell DNA hybridization Cell immobilization Microelectrode array

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Editors' Choice-Critical Review-A Critical Review of solid state Gas sensors

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JOURNAL OF THE ELECTROCHEMICAL SOCIETY 2020年第3期167卷

作者： Hunter, Gary W. Akbar, Sheikh Bhansali, Shekhar Daniele, Michael Erb, Patrick D. Johnson, Kevin Liu, Chung-Chiun Miller, Derek Oralkan, Omer Hesketh, Peter J. Manickam, Pandiaraj Vander Wal, Randy L. NASA Glenn Res Ctr Cleveland OH 44134 USA Ohio State Univ Columbus OH 43210 USA Florida Int Univ Miami FL 33174 USA North Carolina State Univ Raleigh NC 27695 USA Univ North Carolina Chapel Hill Chapel Hill NC 27599 USA US Naval Res Lab Washington DC 20375 USA Case Western Reserve Univ Cleveland OH 44106 USA Georgia Inst Technol Atlanta GA 30332 USA CSIR Cent Electrochem Res Inst Karaikkudi 630003 Tamil Nadu India Penn State Univ State Coll PA 16801 USA

solid state gas sensors are a core enabling technology to a range of measurement applications including industrial, safety, and environmental monitoring. The technology associated with solid-state gas sensors has evolved in recent years with advances in materials, and improvements in processing and miniaturization. In this review, we examine the state-of-the-art of solid state gas sensors with the goal of understanding the core technology and approaches, various sensor design methods to provide targeted functionality, and future prospects in the field. The structure, detection mechanism, and sensing properties of several types of solid state gas sensors will be discussed. In particular, electrochemical cells (solid and liquid), impedance/resistance based sensors (metal oxide, polymer, and carbon based structures), and mechanical sensing structures (resonators, cantilevers, and acoustic wave devices) as well as sensor arrays and supporting technologies, are described. development areas for this field includes increased control of material properties for improved sensor response and durability, increased integration and miniaturization, and new material systems, including nano-materials and nano-structures, to address shortcomings of existing solid state gas sensors. (c) 2020 The Author(s). Published on behalf of The Electrochemical Society by IOP Publishing Limited.

关键词： Metals

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Signal Evaluation of Gas sensors with Artificial Neural Nets

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IFAC Proceedings Volumes 1997年第7期30卷 363-368页

作者： Hanns-Erik Endres Fraunhofer-Institute Solid State Technology Hansastraße 27d D-80686 München Germany Tel +49 (089) 54759-223 Fax +49 (089) 54759-100

Due to the lack in selectivity and separability of most common gas sensors, the use of sensor arrays together self adapting systems as artificial neural nets (ANN) is necessary. These systems can evaluate the gas concentration or deliver a binary signal (gas present or not, ventilation on or off, etc.), which gives a chance to solve measurement problems in gas mixtures. Also the estimation of indoor air quality, olfactometric measurements, etc. may be possible using ANN methods. A promising new approach is the use of non-stationary (transient) signals of a single sensor after a certain stimulus (i.e. a temperature change, etc.). This additional information enhances the redundancy of such a system and a single sensor delivers further information (virtual sensor array). Signal evaluation methods for chemical sensors are powerful tools, they are adaptable to specific applications and are compatible to customary micro controllers.

关键词： Gas sensor Semiconductor sensor Neural Networks Signal Processing Algorithms Air Quality Process Control Olfactometry Gas Warning

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Fishbone architecture: an equivalent mechanical model of cochlea and its application to sensors and actuators

Fishbone architecture: an equivalent mechanical model of coc...

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International Conference on solid state sensors and Actuators (TRANSDUCERS)

作者： S. Ando K. Tanaka M. Abe University of Tokyo Bunkyo Tokyo Japan University of Tokyo Japan

By focusing on the sophisticated functions of the mammalian cochlea, we are aiming to apply its resonator array structure to highly efficient smart sensors and actuators. In this paper, we propose an equivalent mechanical model named "fishbone architecture" which is easily realized by Si micromachining. applications of the architecture to a sensor-artificial basilar membrane (BM) microphone (ABMM)-and an actuator-"giant impulse generator"-are proposed and examined by FEM analyses. The fabrication process and experimental results of the Si device are shown.

关键词： Mechanical sensors Intelligent sensors sensor arrays Biomembranes Frequency Vibrations solid modeling Actuators Coupling circuits Transmission lines

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Design possibilities for photodiode arrays with integral silicon-gate scan generators

Design possibilities for photodiode arrays with integral sil...

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IEEE International Conference on solid-state Circuits (ISSCC)

作者： G. Weckler R. Dyck Fairchild Research and Development Lab. Palo Alto CA USA

A RECENTLY-DESCRIBED single-dimension self-scanning photodiode array has been seminal to a growing number of modified structures for a variety of applications. The MOS shift register, which controls array scanning, has in particular demonstrated considerable versatility and applicability for different array designs. This paper will discuss the tradeoffs in shift register and photodiode structure which set the limits on array performance.

关键词： Photodiodes sensor arrays Shift registers Clocks FETs Capacitance Impedance Sampling methods Diodes Charge transfer

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People count system using multi-sensing application

People count system using multi-sensing application

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International Conference on solid state sensors and Actuators (TRANSDUCERS)

作者： K. Hashimoto K. Morinaka N. Yoshiike C. Kawaguchi S. Matsueda Human Environment Research Laboratory Matsushita Elecrric Indusrrial Company Limited Moriguchi Osaka Japan Kyushu Matsushita Electric Company Limited Sadowara Miyazaki Japan

A people counting system with human information sensors for use in a multi-sensing application for detecting the number of persons and their directions of movement at a wide door has been developed. The sensor head module of this sensing system consists of a 1-dimensional 8-element detector array fabricated by a sheet forming method of pyroelectric PbTiO/sub 3/ ceramics, a IR-transparent lens and oscillating mechanical chopping parts. The sensor output is binarized by using the background mean method, and the number of passing persons and their directions of movement are recognized by a pattern recognition algorithm. By using this people counting system, the number of passersby at a 200 cm wide door can be detected with more than 95% accuracy.

关键词： sensor arrays Mechanical sensors Pattern recognition Humans sensor systems and applications sensor systems Detectors Pyroelectricity Ceramics Lenses

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A high resolution electron imaging integrated circuit

A high resolution electron imaging integrated circuit

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European Conference on solid-state Circuits (ESSCIRC)

作者： D.G. Lomas J.V. Hatfield Department of Electrical Engineering & Electronics UMIST (University of Manchester Institute of Science and Technology) Manchester UK

This paper reports the development of a new kind of two dimensional high resolution electron imaging integrated circuit. It is used in conjunction with Microchannel Plate electron multiplier arrays for image amplification. The High-resolution Electron Detector Array IC (HEDAIC) consists of a two dimensional array of 256 × 256 nMOS charge sensing circuits, each covered by its own 20µm × 20µm aluminium electrode on the IC surface. These electrodes are used to collect the electron charge from the MCP channel outputs. The sensors are arranged and accessed in a RAM like manner, and can be configured to generate either an analogue or digital output.

关键词： Image resolution High-resolution imaging sensor arrays Electrodes Microchannel Electron multipliers Detectors MOS devices Circuits Aluminum

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A biomedical multiprocessor SoC for closed-loop neuroprosthetic applications

A biomedical multiprocessor SoC for closed-loop neuroprosthe...

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IEEE International Conference on solid-state Circuits (ISSCC)

作者： Tung-Chien Chen Kuanfu Chen Zhi Yang Kimberly Cockerham Wentai Liu National Taiwan University Taipei Taiwan University of California Santa Cruz CA Stanford University Stanford CA

Successful proof-of-concept laboratory experiments on cortically controlled motor prostheses, brain pacemakers and hippocampal prostheses motivate continued development for neural prosthetic systems. Advances in implantable electrode arrays and miniaturized multichannel recording ICs enable long-duration, wireless and closed-loop experiments on freely moving subjects. To further improve clinically viable neural prostheses, the bulk associated with external systems must be eliminated. Thus a miniaturized processing-and-controlling system interfacing recording ICs and actuators in real time is required.

关键词： Neural prosthesis Sorting Principal component analysis Epilepsy Prosthetics sensor arrays Covariance matrix Logic gates Feature extraction Implants

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sensor systems-interface between environment and application

Sensor systems-interface between environment and application

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European Conference on solid-state Circuits (ESSCIRC)

作者： D. Hammerschmidt Fraunhofer Institute of Microelectronic Circuits and System Duisburg Germany

The rapidly growing market for sensor systems on the one hand boosts the development of new sensor principles and technologies [1,2], on the other hand the increasing fields of use require an integration of these sensors on system level under a wide spread of application conditions. This paper will discuss the main tasks that have to be solved by the electronic system, which interfaces the sensor and the application environment [3,4]. On side of the sensor physical points of view dominate the requirements, which, e.g., call for low noise readout, high gain, minimum sensor loading, and compensation of crossensitivities. The application requires completely different considerations, like compatibility with former solutions, standardized interfaces, low power consumption or networking capability as well as cost efficiency. Between these completely different classes of requirements electronic circuitry operates as a mediator featuring sophisticated readout electronics for each type of sensor, intermediate signal processing layers, and customisable interfaces that are usable for all kinds of sensors. After the general consideration of the above topics some examples of sensor systems will be presented and the individual solutions of the mentioned challenge will be discussed from the circuit designers point of view. At last some approaches for future developments of sensor systems will be presented.

关键词： sensor systems sensor arrays Magnetic sensors sensor systems and applications Chemical sensors Costs Consumer electronics Circuits Pressure measurement Manufacturing

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development of micromachined switches with increased reliability

Development of micromachined switches with increased reliabi...

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International Conference on solid state sensors and Actuators (TRANSDUCERS)

作者： K.M. Hiltmann B. Schmidt H. Sandmaier W. Lang Hahn Schickard Society Institute of Micro and Informationtechnology Villingen-Schwenningen Germany

By micromachining of silicon and glass, an electrical switch with low-resistance metal contacts was made. The switch is aimed at applications as a binary pressure sensor and to replace conventional push-button switches. First measurements on switches yielded electrical lifetimes over 10/sup 5/ cycles and contact resistance clearly below 1 /spl Omega/.

关键词： Switches Silicon Glass Bonding Switching circuits Surface morphology Biomembranes Contact resistance sensor systems solid state circuits

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