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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是101-110 订阅

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Gabor wavelet transform for dynamic analysis in digital holographic microscopy

Gabor wavelet transform for dynamic analysis in digital holo...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Zhong, Jingang Weng, Jiawen Hu, Cuiying Jinan Univ Inst Optoelect Engn Guangzhou 510632 Guangdong Peoples R China

ISBN: (纸本)9780819479662

Real time dynamic analysis of micro-object is one of the significant advantages of digital holographic microscopy, but the process of the spatial filtering limits the application of dynamic and automatic analysis in digital holographic microscopy. In this paper, a numerical reconstruction technique by means of Gabor wavelet transform (GWT) is presented for real time dynamic analysis. Appling the GWT to the hologram, the quantitative phase information of the micro-object is obtained automatically without the process of the spatial filtering. An experiment with a sequence of holograms of an animalcule is proposed for the dynamic and automatic analysis by employing the GWT method.

关键词： Digital holographic microscopy dynamic analysis wavelet transform full-field quantitative phase imaging

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In-line digital holographic microscopy based on intensity measurements at two planes

In-line digital holographic microscopy based on intensity me...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Das, Bhargab Yelleswarapu, Chandra S. Rao, D. V. G. L. N. Univ Massachusetts Dept Phys Boston MA 02125 USA

ISBN: (纸本)9780819484413

We propose a new approach of in-line digital holographic microscopy (DHM) with the capability of enhancing the hologram acquisition rate together with improved reconstruction capability. The method is based on the recording of two interferograms of the same object at slightly different planes. The technique utilizes the full spatial bandwidth of the camera and do not require phase-shifting of the reference beam. Furthermore, we exploit the method of subtraction of average intensity of the entire hologram to suppress the zero-order diffracted wave. The twin image is eliminated by Fourier domain processing of the two recorded holograms. Experimental results of both amplitude and phase objects demonstrate the feasibility of this method. Since the two interferograms can be recorded simultaneously by using two CCD or CMOS sensors, this new in-line DHM technique has the potential applications in biomedical research for the visualization of rapid dynamic processes at cellular level.

关键词： Digital holography microscopy Numerical Reconstruction Quantitative Phase Imaging

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Tomographic imagery by interference microscopy

Tomographic imagery by interference microscopy

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Dubois, A Guiot, E Schwartz, W Moneron, G Grieve, K Boccara, C CNRS UPR A0005 Ecole Super Phys & Chim Ind Lab Opt Phys F-75231 Paris France

ISBN: (纸本)0819456756

We have developed a white-light interference microscope as an alternative technique to conventional optical coherence tomography (OCT). The experimental setup is based on a Linnik interferometer illuminated with a tungsten halogen lamp. En face tomographic images are obtained in real-time without scanning by computing the difference of two phase-opposed interferometric images recorded by a CCD camera. The short coherence length of the source yields an optical sectioning ability with 0.7 mu m resolution (in water). Transverse resolution of 0.9 mu m is achieved by using high numerical aperture microscope objectives. A shot-noise limited detection sensitivity of 90 dB can be reached with similar to 1 s acquisition time. High-resolution images of mouse and tadpole embryos are shown.

关键词： interference microscopy three-dimensional microscopy optical coherence tomography medical and biomedical imaging

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Fast 3D blind deconvolution of even point spread functions

Fast 3D blind deconvolution of even point spread functions

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Yagle, AE Shah, S Univ Michigan Dept EECS Ann Arbor MI 48109 USA

ISBN: (纸本)0819452327

3-D mages obtained from optical sectioning microscopy are usually degraded by a point-spread function that is known to be an even function but is otherwise only approximately known, or even entirely unknown. We present a new algorithm for 3-D blind deconvolution of even point-spread functions that is both fast and (in the absence of noise) exact. Fourier transforms decouple the problem into 2-D, then 1-D blind deconvolution problems, greatly increasing computational speed. Numerical simulations demonstrate that the blind algorithm seems to perform both faster and more accurately than the non-blind iterative Lucy-Richardson algorithm.

关键词： Deconvolution Point spread functions 3D image processing Signal to noise ratio Convolution microscopy Fourier transforms Confocal microscopy image filtering Matrices

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Tracking fluorescent spots in biological video microscopy

Tracking fluorescent spots in biological video microscopy

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Genovesio, A Olivo-Marin, JC Inst Pasteur Quantitat Image Anal Grp F-75724 Paris France

ISBN: (纸本)0819447641

The analysis of cell and pathogen movement and motility is a major topic in cell biology for which computerized methods are most needed. This study proposes a method to detect and track multiple moving biological objects in image sequences acquired through fluorescence video microscopy. The method enables the analysis of video microscopy image sequences in order to obtain reliable quantitative data such as number, position, speed and movement phases. The method consists of three stages. A stage of detection is performed through a multi-scale analysis of images using an undecimated wavelet transform. The next stage is the prediction of the state of each detected spot in the next frame using a Kalman filter and an adapted model. Then comes a stage of data association which constructs the tracks and refines the filters. Once all moving objects have been assigned with unique spatio-temporal paths, trajectories are analyzed in terms of different parameters relevant to the motility analysis of biological objects.

关键词： image processing tracking wavelets Kalman filter data associations biological video microscopy fluorescent spots

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A study for accelerating the speed of all-in-focus image processing 27

A study for accelerating the speed of all-in-focus image pro...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Wang, Lihui Tabata, Satoshi Takeuchi, Hirotoshi Ishikawa, Masatoshi Guangdong Acad Sci Guangdong Inst Semicond Ind Technol 363 Changxing Rd Guangzhou 510650 Peoples R China Univ Tokyo Grad Sch Informat Sci & Technol Bunkyo Ku 7-3-1 Hongo Tokyo 1138656 Japan

ISBN: (纸本)9781510632547

To capture an all-in-focus and 3D depth image, shape from focus method is widely used. The phase accuracy of the image candidates should be processed and adjusted beforehand Phase only correction method was employed in this work and to accelerate the processing speed, the image processing by Fast Fourier Transform (FFT) was optimized Meanwhile, the processing task was assigned in several parallel threads so that the performance would be improved. The method was used on a variable focus imaging system, and, as a result, the processing speed was improved to around 2.5-fps.

关键词： all-in-focus shape from focus phase-only correction method 3D imaging variable focus system

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Use of bessel beams and position-sensitive detectors in scanning photon microscope for improved field depth and contrast

Use of bessel beams and position-sensitive detectors in scan...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Khmaladze, Alexander Kim, Myung K. Univ S Florida Dept Phys 4202 E Fowler Ave Tampa FL 33620 USA

ISBN: (纸本)0819461326

The scanning photon microscope technique is a method of microscopic image formation that employs a laser beam focused on a sample, while non-imaging detector receives the scattered light. The scans are achieved by means of a galvanometer based scanning mirror and a motorized micrometer. The system produces images analogous to the scanning electron microscopy with three-dimensional effects of shadowing and reflection. Compared to a conventional wide-field imaging system, the method allows for a greater ease of operation and flexibility, as the image quality is dependent upon the characteristics of the laser beam, rather than imaging optics. The image resolution on the order of a micron is demonstrated. A further gain in terms of resolution and the depth of focus by employment of Bessel rather than Gaussian beams is discussed. Additionally, we used a position-sensitive quadrant photodiode detector to highlight the overall spatial orientation of the imaged surface as well as its roughness. This concept can be useful in many areas, such as coherence imaging and fluorescence.

关键词： scanning microscopy axicon surface profile

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Confocal endoscopy

Confocal endoscopy

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Karadaglic, D Juskaitis, R Wilson, T Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

ISBN: (纸本)0819447641

We describe a simple modification to a rigid endoscope so as to provide both high quality conventional endoscopic as well as and confocal endoscopic images of reasonably accessible regions of the body in real time. The systems are based around either host lenslet-array tandem scanning microscope together with laser illumination or a structured illumination approach together with a conventional incoherent illumination source. images taken in fluorescence are presented using this combined conventional and confocal endoscope.

关键词： Confocal microscopy Endoscopes Microscopes Endoscopy Luminescence Optics manufacturing Tissue optics Tissues image quality Surgery

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Heart Synchronization for SPIM microscopy of Living Zebra Fish

Heart Synchronization for SPIM Microscopy of Living Zebra Fi...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Taylor, J. M. Saunter, C. D. Chaudhry, B. Henderson, D. J. Love, G. D. Girkin, J. M. Univ Durham Dept Phys Ctr Adv Instrumentat South Rd Durham DH1 3LE England Newcastle Univ Inst Human Genet Newcastle Upon Tyne NE1 3BZ Tyne & Wear England

We describe work on producing a selective plane illumination microscope for cardiac imaging in zebra fish embryos. The system has a novel synchronization system for imaging oscillating structures (e.g. the heart) and ... 详细信息

ISBN: (纸本)9780819484413

关键词： Optical microscopy Selective Plane Illumination Synchronization

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Multi-wavelength digital holographic tomography based on spectral interferometry

Multi-wavelength digital holographic tomography based on spe...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVI

作者： Yu, Lingfeng Chen, Zhongping Univ Calif Irvine Dept Biomed Engn Beckman Laser Inst Irvine CA 92612 USA

ISBN: (纸本)9780819474308

We report a multi-wavelength digital holographic tomography system based on a fiber-based spectral interferometer. Instead of using tunable lasers, a relatively inexpensive broadband source was used as the light source. Hundreds of 2-D "synthesized holograms" (or object wavefields) were obtained by transversal scanning of a probe beam within a few seconds. Holographic images of an object volume were numerically reconstructed from each synthesized hologram and tomographic images were obtained by superposition of all the image volumes.

关键词： Digital holography Tomography Holograms Holography 3D image reconstruction Holographic interferometry Laser beam diagnostics Reconstruction algorithms 3D image processing Interferometry

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