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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是111-120 订阅

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three dimensional imaging using phase retrieval with two focus planes 13

Three dimensional imaging using phase retrieval with two foc...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Ilovitsh, Tali Ilovitsh, Asaf Weiss, Aryeh Meir, Rinat Zalevsky, Zeev Bar Ilan Univ Fac Engn IL-5290002 Ramat Gan Israel Bar Ilan Univ Bar Ilan Inst Nanotechnol & Adv Mat IL-5290002 Ramat Gan Israel

ISBN: (纸本)9781628419474

This work presents a technique for a full 3D imaging of biological samples tagged with gold-nanoparticles (GNPs) using only two images, rather than many images per volume as is currently needed for 3D optical sectioning microscopy. The proposed approach is based on the Gerchberg-Saxton (GS) phase retrieval algorithm. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. In addition, since the method requires the capturing of two images only, it can be suitable for 3D live cell imaging. The proposed concept is presented and validated both on simulated data as well as experimentally.

关键词： three-dimensional microscopy Cellular imaging gold nanoparticle Phase retrieval

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Novel contrast mechanism for label free super-resolution imaging 13

Novel contrast mechanism for label free super-resolution ima...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Alexandrov, Sergey A. McGrath, James Subhash, Hrebesh Boccafoschi, Francesca Giannini, Cinzia Leahy, Martin Natl Univ Ireland Sch Phys Tissue Opt & Microcirculat Imaging Grp Galway Ireland Univ Piemonte Orientale Dept Hlth Sci I-28100 Novara Italy CNR Inst Crystallog Via Amendola 122-O I-70126 Bari Italy

ISBN: (纸本)9781628419474

We present a novel super-resolution spectral encoding of spatial frequency (srSESF) approach to break the diffraction limit and dramatically improve resolution in lateral direction. The idea is to utilize additional information about the internal structure of the object to resolve features in the lateral direction. The novel contrast mechanism is realized by reconstruction and comparison of the axial spatial frequency (period) profiles at each image point to form superresolution image. As a result, small features, unresolved by conventional microscopy, can be visualized. Numerical simulation and experiments confirm the super-resolution abilities of the srSESF approach without applying any labels.

关键词： Optical microscopy label-free imaging super-resolution

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Confocal imaging with orthogonally polarized illumination beams 13

Confocal imaging with orthogonally polarized illumination be...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Kalita, Ranjan Boruah, Bosanta R. Indian Inst Technol Guwahati Dept Phys Gauhati Assam India

ISBN: (纸本)9781628419474

In confocal microscopy the polarization of the illumination beam plays an important role in determining the orientation of the fluorescent molecules being illuminated. The efficiency of the excitation depends on the angle between the excitation electric field and the direction of the molecular dipole. In order to determine the orientation of the fluorescent molecules in the focal plane the molecules are to be excited using two mutually orthogonal electric fields. In this paper we show how a computer generated holography technique can be implemented using a ferroelectric liquid crystal spatial light modulator to conveniently obtain two images of the same target once with an X polarized illumination beam and another with a Y polarized illumination beam.

关键词： Confocal microscopy Computer generated holography Polarization Molecular orientation

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Super-resolution optical microscopy by using dielectric microwires 13

Super-resolution optical microscopy by using dielectric micr...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Darafsheh, Arash Wu, Gaoxiang Yang, Shu Finlay, Jarod C. Univ Penn Dept Radiat Oncol Philadelphia PA 19104 USA Univ Penn Dept Mat Sci & Engn 3231 Walnut St Philadelphia PA 19104 USA

ISBN: (纸本)9781628419474

We demonstrate that super-resolution imaging of specimens containing sub-diffraction-limited features is feasible by using dielectric microwires fabricated through capillary force lithography followed by photopatterning. As supplementary micron scale cylindrical lenses, we fabricated uniform-sized microwires with and 5 and 10 mu m diameters and refractive index similar to 1.3-1.6. The microwires are placed in contact with the specimen to collect the information of the sub-wavelength features of the specimen and transmit them to the far-field with magnification enabling imaging with two-fold resolution improvement. Potential applications of our imaging technique include biological imaging, microfluidics, and nanophotonics applications.

关键词： Super-resolution microscopy nanoscopy microwire

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Application of linear-scale differential analysis in phase correlation method of image stitching 13

Application of linear-scale differential analysis in phase c...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Bezzubik, Vitalii V. Belashenkov, Nickolai R. Losev, Sergei V. ITMO Univ Kronverksky Pr 49 St Petersburg 197101 Russia

ISBN: (纸本)9781628419474

A robust method of creation of panoramic images which does not consume much processing resources is proposed and investigated. The phase correlation (PC) method is taken as a basic one because of simplicity of its algorithm and low computing time due to application of FFT technique. Standard PC procedure is modified by preprocessing of source frames of panoramic images in spatial domain. Preprocessing comprises Linear-Scale Differential Analysis (LSDA) with sequent content-dependent thresholding of intensity gradients. Method is proved for artificially blurred and noise corrupted images. It is shown that new robust algorithm allows to increase the productivity of creation of panoramic images keeping the probability of successive stitching close to maximum even for low-quality source frames.

关键词： Digital image image stitching phase correlation linear-scale differential analysis

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3D imaging of the cleared intact murine colon with light sheet microscopy 13

3D imaging of the cleared intact murine colon with light she...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Zufiria, B. Bocancea, D. I. Gomez-Gaviro, M. V. Vaquero, J. J. Desco, M. Fresno, M. Ripoll, J. Arranz, A. Univ Autonoma Madrid CSIC Ctr Mol Biol Severo Ochoa CBMSO Madrid Spain Univ Carlos III Madrid Dept Bioengn & Aerosp Engn Madrid 28911 Spain Inst Invest Sanitaria Gregorio Maranon Madrid 28007 Spain

ISBN: (纸本)9781628419474

We here show 3D light sheet microscopy images of fixed and cleared murine colon tissue in-toto, which offer relevant cellular information without the need for physically sectioning the tissue. We have applied the recently developed CUBIC protocol (Susaki et al. Cell 157: 726, 2014) for colon tissues and have found that this clearing protocol enables imaging all the way to the central part of the lumen with cellular resolution, thus opening new ways for 3D imaging of colon samples

关键词： light sheet microscopy CUBIC protocol tissue clearing

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Lensfree on-chip high-resolution imaging using two-way lighting, and its limitations 13

Lensfree on-chip high-resolution imaging using two-way light...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Adachi, Yasuhiko Tamaki, Tokuhiko Motomura, Hideto Kato, Yoshihisa Panasonic Corp Adv Res Div Osaka Japan Panasonic Corp Automot & Ind Syst Co Div Engn Kyoto Japan

ISBN: (纸本)9781628419474

A high-magnification image of a biological sample can generally be obtained by an optical microscope with an objective lens, moving the image sensor with a sub-pixel shift and the subsequent image processing for super-resolution. However, to obtain a high-resolution image, a large number of images will be required for the super-resolution, and thus it is difficult to achieve real-time operation, and the field-of-view (FOV) is not sufficiently wide. The currently proposed digital holography technique places a sample on the image sensor and captures the interference fringe (hologram) to reconstruct a 3D high-resolution image in a computer. This technique ensures the features of a wide FOV, whereas the high resolution obtained by image processing cannot ensure real-time operation, because it requires recursive calculations of light propagation and adequate computer resources. To realize wide FOV and the real-time operation at the same time, we have developed a new technique: Lensfree on-chip high-resolution imaging using two-way lighting. High-resolution image is immediately obtained by image processing of the low-resolution images of the samples. This makes it possible to ensure a wide FOV, a deep depth of focus without the need for focus adjustment, and a continuously expanding operation. We also discuss the limitations of the high resolution.

关键词： Lensfree microscopy On-chip microscopy Two-way lighting

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microscopy using source and detector arrays 13

Microscopy using source and detector arrays

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Sheppard, Colin J. R. Castello, Marco Vicidomini, Giuseppe Duocastella, Marti Diaspro, Alberto Ist Italiano Tecnol Nanoscopy Nanophys Via Morego 30 I-16163 Genoa Italy Univ Genoa DIBRIS Via Opera Pia 13 I-16145 Genoa Italy

ISBN: (纸本)9781628419474

There are basically two types of microscope, which we call conventional and scanning. The former type is a full-field imaging system. In the latter type, the object is illuminated with a probe beam, and a signal detected. We can generalize the probe to a patterned illumination. Similarly we can generalize the detection to a patterned detection. Combining these we get a range of different modalities: confocal microscopy, structured illumination (with full-field imaging), spinning disk (with multiple illumination points), and so on. The combination allows the spatial frequency bandwidth of the system to be doubled. In general we can record a four dimensional (4D) image of a 2D object (or a 6D image from a 3D object, using an acoustic tuneable lens). The optimum way to directly reconstruct the resulting image is by image scanning microscopy (ISM). But the 4D image is highly redundant, so deconvolution-based approaches are also relevant. ISM can be performed in fluorescence, bright field or interference microscopy. Several different implementations have been described, with associated advantages and disadvantages. In two-photon microscopy, the illumination and detection point spread functions are very different. This is also the case when using pupil filters or when there is a large Stokes shift.

关键词： Confocal microscopy scanning optical microscopy fluorescence microscopy image scanning microscopy pixel reassignment

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New Approaches for the analysis of confluent cell layers with quantitative phase digital holographic microscopy 13

New Approaches for the analysis of confluent cell layers wit...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Pohl, L. Kaiser, M. Ketelhut, S. Pereira, S. Goycoolea, F. Kemper, Bjoern Univ Munster Fac Med Biomed Technol Ctr Mendelstr 17 D-48149 Munster Germany Univ Munster Inst Plant Biol & Biotechnol Schlossgarten 3 D-48149 Munster Germany

ISBN: (纸本)9781628419474

Digital holographic microscopy (DHM) enables high resolution non-destructive inspection of technical surfaces and minimally-invasive label-free live cell imaging. However, the analysis of confluent cell layers represents a challenge as quantitative DHM phase images in this case do not provide sufficient information for image segmentation, determination of the cellular dry mass or calculation of the cell thickness. We present novel strategies for the analysis of confluent cell layers with quantitative DHM phase contrast utilizing a histogram based-evaluation procedure. The applicability of our approach is illustrated by quantification of drug induced cell morphology changes and it is shown that the method is capable to quantify reliable global morphology changes of confluent cell layers.

关键词： digital holographic microscopy quantitative phase imaging live cell imaging minimally-invasive label-free stain-free confluent cell layers

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A linear algorithm for quantitative measure of corneal collagen fiber orientation using second harmonic generation microscopy 13

A linear algorithm for quantitative measure of corneal colla...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： McLean, James DiMarzio, Charles Northeastern Univ 360 Huntington Ave Boston MA 02115 USA

ISBN: (纸本)9781628419474

There is significant interest in the scientific community to develop a reliable and precise quantification of the direction of collagen fibers within the cornea in order to learn more about how collagen contributes to the cornea's shape and structure. Previous work has shown that quantification of these fibers' orientations is possible using Second Harmonic Generation (SHG) microscopy, a modality that utilizes the non-centrosymmetric properties of collagen to obtain details of their macromolecular structure. Many attempts at using SHG to this purpose result in whole volume approximations which do not take into account variations through the depth of the cornea. Additionaly, other algorithms have used non-linear processes which limit computation time and result in sensitivity to sample area, resolution, and illumination. We propose a linear method for quantification of collagen fiber orientation which utilizes the precise sectioning properties of SHG and is independent of non-linear artifacts to aid in further classification of cornea structure.

关键词： Second Harmonic Generation collagen cornea orientation quantification

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