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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是111-120 订阅

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Single image sectioning using spatial frequency domain imaging 24

Single image sectioning using spatial frequency domain imagi...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIV

作者： Hoffman, Zachary R. DiMarzio, Charles A. Northeastern Univ 360 Huntington Ave Boston MA 02115 USA Draper Lab 555 Technol Sq Cambridge MA 02139 USA

ISBN: (数字)9781510605824

ISBN: (纸本)9781510605817;9781510605824

Structured illumination microscopy (SIM) works well to produce depth information by removing undesired light from out of focus planes within a specimen. However, it generally requires multiple images in order to produce sectioning. We show that, under the right conditions, we can leverage Spatial Frequency Domain Imaging (SFDI) to produce both sectioning and relative depth information about a specimen using only a single image. After processing, we find that the axial resolution is comparable to that of traditional 3 phase SIM. With this technique, we are able to produce real-time videos in-vivo at 21 frames per second. Additionally, no moving parts are needed to produce sectioning at depth.

关键词： Structured Illumination optical sectioning super-resolution incoherent SFDI

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High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

High speed slit-scanning confocal laser microscopy with an a...

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Han, SM Im, KB Park, H Kim, D Kim, BM Yonsei Univ Dept Biomed Engn Kangwon Do 220710 South Korea

ISBN: (纸本)0819456756

Using an acousto-optic beam deflector and a line scan camera, we constructed a non-mechanical, slit-scanning confocal microscope. It generates two-dimensional 512x512 images with speed of 60 frames/sec, which can be easily expanded, to 100 frames/sec. The measured axial and lateral resolutions of the system are 3.3 mu m and < 1 mu m, respectively, with a 50X objective. This simple design can produce frames rates faster than other commercial products with comparable resolution, which may be useful for analysis of rapid interactions in various biological applications.

关键词： confocal microscope three-dimensional microscopy acousto-optic deflector line scan CCD

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Star Test Polarimetry using Stress-Engineered Optical Elements

Star Test Polarimetry using Stress-Engineered Optical Elemen...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Ramkhalawon, Roshita Beckley, Amber M. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA

ISBN: (纸本)9780819488701

Stress-engineered optical elements have potential applications in snapshot polarimetry, in which a single irradiance image is used to measure a spatially varying polarization. In this paper, we present star test polarimetry which is a method of polarization retrieval by analyzing a single frame point spread function. A trigonally stressed window placed at the pupil plane of an imaging system is used to produce point spread functions which are then processed to extract the polarization state of the incoming beam under investigation. We outline several methods which are used to recover the Stokes parameters of a beam of unknown polarization from the irradiance distribution of its point spread function.

关键词： Polarimetry microscopy Unconventional Polarization states

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Microscopic tomography by digital interference holography

Microscopic tomography by digital interference holography

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Kim, MK Univ S Florida Dept Phys Tampa FL 33620 USA

ISBN: (纸本)0819452327

We are developing the digital interference holography (DIH) as a novel method of microscopic tomographic imaging by numerical superposition of a number of holographic fields taken with varying wavelengths. The digital interference holography does not involve pixel-by-pixel mechanical scanning of three-dimensional volume and yet achieves high lateral and longitudinal resolutions. The holographic interference pattern of an ob ect is generated optically and recorded digitally using a CCD camera. The hologrpahic image field is numerically calculated using basic diffraction formulas and the process is repeated for a range of varying wavelengths at regular intervals. Numerical superposition, or digital interference, of the holographic image fields yields the desired three-dimensional representation of the object. Experiments have demonstrated a few-micron lateral and axial resolutions. Furthermore, since the DIH is a coherent imaging system, one can form true tomographic images of sub-surface structures, in the presence of diffuse scattering from overlying layers. By being able to generate true tomographic images of subsurface structures, without the need for three-dimensional mechanical scannig, the DIH method can provide a very efficient and versatile imaging modality for a wide range of applications in materials science and biomedical imaging.

关键词： digital holography tomography microscopy multi-wavelength imaging

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In-line digital holographic microscopy based on intensity measurements at two planes

In-line digital holographic microscopy based on intensity me...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Das, Bhargab Yelleswarapu, Chandra S. Rao, D. V. G. L. N. Univ Massachusetts Dept Phys Boston MA 02125 USA

ISBN: (纸本)9780819484413

We propose a new approach of in-line digital holographic microscopy (DHM) with the capability of enhancing the hologram acquisition rate together with improved reconstruction capability. The method is based on the recording of two interferograms of the same object at slightly different planes. The technique utilizes the full spatial bandwidth of the camera and do not require phase-shifting of the reference beam. Furthermore, we exploit the method of subtraction of average intensity of the entire hologram to suppress the zero-order diffracted wave. The twin image is eliminated by Fourier domain processing of the two recorded holograms. Experimental results of both amplitude and phase objects demonstrate the feasibility of this method. Since the two interferograms can be recorded simultaneously by using two CCD or CMOS sensors, this new in-line DHM technique has the potential applications in biomedical research for the visualization of rapid dynamic processes at cellular level.

关键词： Digital holography microscopy Numerical Reconstruction Quantitative Phase Imaging

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Confocal endoscopy

Confocal endoscopy

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Karadaglic, D Juskaitis, R Wilson, T Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

ISBN: (纸本)0819447641

We describe a simple modification to a rigid endoscope so as to provide both high quality conventional endoscopic as well as and confocal endoscopic images of reasonably accessible regions of the body in real time. The systems are based around either host lenslet-array tandem scanning microscope together with laser illumination or a structured illumination approach together with a conventional incoherent illumination source. images taken in fluorescence are presented using this combined conventional and confocal endoscope.

关键词： Confocal microscopy Endoscopes Microscopes Endoscopy Luminescence Optics manufacturing Tissue optics Tissues image quality Surgery

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Wide-Field Time-Correlated Single Photon Counting imaging for luminescence microscopy

Wide-Field Time-Correlated Single Photon Counting imaging fo...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Sergent, Nicolas Levitt, James A. Green, Mark A. Suhling, Klaus Kings Coll London Dept Phys London WC2R 2LS England

ISBN: (纸本)9780819470362

We demonstrate Wide-Field Time-Correlated Single Photon Counting (WiFi TCSPC) imaging based on an image intensifier and a high-speed camera running at 30,000 frames per second. The timing of photon events is thus performed in parallel, simultaneously on every pixel. The system is applied to lanthanide lifetime measurements and time-resolved imaging of the lanthanide complex Europium Polyoxometalate (Eu POMs). We measure a lifetime of 2.98 ms for Eu POMs in solid state, which is in excellent agreement with the literature value.

关键词： Time-Correlated Single Photon Counting Lifetime Imaging microchannel plate TCSPC

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Point spread functions for particle imaging using inhomogeneous polarization in scanning optical microscopy

Point spread functions for particle imaging using inhomogene...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Youngworth, KS Biss, DP Brown, TG Univ Rochester Inst Opt Rochester NY 14627 USA

ISBN: (纸本)0819439398

Polarization control and analysis continue to be a useful tools for microscopy. We have previously addressed the use of spatially inhomogeneous polarization, specifically azimuthal polarization, in scanning optical microscopy. In this paper we describe new techniques for generating both azimuthally and radially polarized cylindrical-vector beams, investigate high numerical aperture focusing, compare the theoretical and experimental point spread functions for small particle imaging, and explore the use of these beams with microscopy.

关键词： radial polarization azimuthal polarization inhomogeneous polarization cylindrical-vector beams axial electric field high numerical aperture focusing scanning optical microscopy point spread function small particle imaging

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High-resolution volumetric imaging using hybrid image of Fourier light-field microscopy and wide-field microscopy 29

High-resolution volumetric imaging using hybrid image of Fou...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXix

作者： Liu, Wenhao Jia, Shu Georgia Inst Technol Wallace H Coulter Dept Biomed Engn Atlanta GA 30332 USA Emory Univ Atlanta GA 30332 USA

ISBN: (纸本)9781510648043;9781510648036

We have developed wFLFM to further enhance the resolution by taking the advantage of both wide-field image and FLFM. We proposed the principle of wFLFM and verified the approach both numerically using simulated rings, and experimentally using phantom samples on the wFLFM system we constructed as a prototype. The principle of wFLFM requires only additional wild-field image at the aperture plane, making it readily compatible with various extended depth of focus wide-field modules, and the validating results showed a 2-3x enhancement in lateral resolution without cost in axial information, thereby advancing wFLFM as a promising high-resolution 3D imaging method.

关键词： Light-field microscopy Fourier light-field microscopy high resolution volumetric imaging

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Tomographic imagery by interference microscopy

Tomographic imagery by interference microscopy

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Dubois, A Guiot, E Schwartz, W Moneron, G Grieve, K Boccara, C CNRS UPR A0005 Ecole Super Phys & Chim Ind Lab Opt Phys F-75231 Paris France

ISBN: (纸本)0819456756

We have developed a white-light interference microscope as an alternative technique to conventional optical coherence tomography (OCT). The experimental setup is based on a Linnik interferometer illuminated with a tungsten halogen lamp. En face tomographic images are obtained in real-time without scanning by computing the difference of two phase-opposed interferometric images recorded by a CCD camera. The short coherence length of the source yields an optical sectioning ability with 0.7 mu m resolution (in water). Transverse resolution of 0.9 mu m is achieved by using high numerical aperture microscope objectives. A shot-noise limited detection sensitivity of 90 dB can be reached with similar to 1 s acquisition time. High-resolution images of mouse and tadpole embryos are shown.

关键词： interference microscopy three-dimensional microscopy optical coherence tomography medical and biomedical imaging

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