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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是121-130 订阅

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Single image structured illumination (SISIM) for in-vivo imaging 25

Single image structured illumination (SISIM) for in-vivo ima...

引用

Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Hoffman, Zachary R. Kivanc, Kose Di Marzio, Charles A. Northeastern Univ 360 Huntington Ave Boston MA 02115 USA Mem Sloan Kettering Canc Ctr 1275 York Ave New York NY 10021 USA

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Recently, it has been demonstrated that sectioning within a turbid media using structured illumination microscopy can be greatly enhanced through the use of a Hilbert transform algorithm. This technique, known as single image structured illumination microscopy (SISIM), provides high quality sectioning even within a strongly refracting/scattering medium, such as tissue. It is found that motion artifacts comprise a large portion of the error in in-vivo imaging. Thus, we expand on the SISIM technique by implementing an image registration algorithm which greatly mitigates motion induced artifacts. Multiple images and movies are developed to demonstrate SISIMs ability to provide real-time, in-vivo imaging of human tissue.

关键词： Structured Illumination optical sectioning thick specimen turbid media incoherent light image registration

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Time-resolved confocal microscopy of cryogenic processes in biological tissues

Time-resolved confocal microscopy of cryogenic processes in ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Schellenberg, M. Bergmann, T. Peev, E. Kloster, M. Napier, J. Neu, W. Univ Appl Sci Emden Leer Dept Nat Sci Constantiapl 4 D-26723 Emden Germany Univ Appl Sci Giessen Friedberg Inst Medizin Phys & Strahlenschutz D-35390 Giessen Germany

ISBN: (纸本)9780819484413

Cryogenic procedures are fundamental tools in modern biology, e. g. for conservation or purification of biological materials. The processes occurring in biological cells and tissues during freezing and thawing are subject to ongoing research. Optimization of cell survival rates demands the development and evaluation of exactly defined temperature profiles. 4D-DMD-microscopy is capable of imaging these highly dynamic processes with high spatial and temporal resolution, utilizing well established staining procedures for differentiating structures of interest.

关键词： two-photon DMD confocal microscopy cryogenic process 4D-microscopy cryomicroscopy

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Coherence measurements applied to critical and Kohler vortex illumination

Coherence measurements applied to critical and Kohler vortex...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVI

作者： Brown, Dean P. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA

ISBN: (纸本)9780819474308

Both coherence and polarization play an important role in microscopy. It was long ago established that, in the context of scalar treatments, Kohler and critical illumination produce equivalent coherence functions in an object plane. This paper examines this equivalence in the context of polarization vortex illumination. Using a reversed-wavefront Young interferometer (RWYI), we show measurements of the coherence and correlation properties of the output illumination plane of various illumination systems. We compare the coherence properties of critical and Kohler vortex illumination schemes and look at the effects these properties have in an imaging system.

关键词： Coherence Polarization Vortex Partial Coherent Imaging Critical Illumination Kohler Illumination Polarization microscopy

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Dynamic phase imaging and processing of moving biological organisms

Dynamic phase imaging and processing of moving biological or...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Creath, Katherine Goldstein, Goldie 4D Technol Corp Tucson AZ 85706 USA

ISBN: (纸本)9780819488701

This paper describes recent advances in developing a new, novel interference Linnik microscope system and presents images and data of live biological samples. The specially designed optical system enables instantaneous 4-dimensional video measurements of dynamic motions within and among live cells without the need for contrast agents. "Label-free" measurements of biological objects in reflection using harmless light levels are possible without the need for scanning and vibration isolation. This instrument utilizes a pixelated phase mask enabling simultaneous measurement of multiple interference patterns taking advantage of the polarization properties of light enabling phase image movies in real time at video rates to track dynamic motions and volumetric changes. Optical thickness data are derived from phase images after processing to remove the background surface shape to quantify changes in cell position and volume. Data from a number of different pond organisms will be presented, as will measurements of human breast cancer cells with the addition of various agents that break down the cells. These data highlight examples of the image processing involved and the monitoring of different biological processes.

关键词： phase imaging interference microscopy polarization interferometry cellular imaging cell dynamics optical thickness measurement label-free imaging 4D microscopy

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Fuzzy Logic Components for Iterative Deconvolution Systems

Fuzzy Logic Components for Iterative Deconvolution Systems

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Northan, Brian M. True North Image Proc Guilderland NY 12084 USA

ISBN: (纸本)9780819493583

Deconvolution systems rely heavily on expert knowledge and would benefit from approaches that capture this expert knowledge. Fuzzy logic is an approach that is used to capture expert knowledge rules and produce outputs that range in degree. This paper describes a fuzzy-deconvolution-system that integrates traditional Richardson-Lucy deconvolution with fuzzy components. The system is intended for restoration of 3D widefield images taken under conditions of refractive index mismatch. The system uses a fuzzy rule set for calculating sample refractive index, a fuzzy median filter for inter-iteration noise reduction, and a fuzzy rule set for stopping criteria.

关键词： Deconvolution Fuzzy Logic Spherical Aberration

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Phase mask optimization for 3D parallax EDF microscopy

Phase mask optimization for 3D parallax EDF Microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Beckers, Ingeborg E. Gierlak, Michael Hoeppel, Robert Landskron, Juergen Beuth Univ Appl Sci Berlin Dept Appl Phys Med Engn D-13347 Berlin Germany

ISBN: (纸本)9780819498625

Extended depth-of-field (EDF) microscopy is a well-investigated and very simple method to obtain projection images with an extended depth of focus. Despite its advantages of being a real-time method applicable to any microscopic mode with high lateral resolution that can be simply realized by extending a commercial microscope, the lack of z-correlation is still a problem. In this work we present a combined technique of EDF and stereomicroscopy. By cross-correlation depth information is obtained. Finally, 3D images are reconstructed for best phase masks and simulation results are evaluated experimentally.

关键词： 3D imaging real time imaging extended depth of field microscopy EDF space light modulation SLM stereoscopy parallax EDFP

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Polarised light microscopy

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Torok, P Higdon, PD Wilson, T Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

ISBN: (纸本)0819427004

In this work we discuss the role of polarisation in confocal microscopy. The effect of the optical system on light polarisation is analysed in the absence of a specimen. We also present numerical results on confocal microscopes imaging small dielectric scatterers and suggest an optimum pinhole size for crossed linear polars. Finally, imaging of large spherical scatterers and contrast optimisation of the optical system is discussed.

关键词： confocal microscopy polarised light vectorial theory high aperture theory

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Evaluation of the use of wavefront encoding to reduce depth-induced aberration in structured illumination microscopy 25

Evaluation of the use of wavefront encoding to reduce depth-...

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Patwary, Nurmohammed Sola-Pikabea, Jorge Doblas, Ana Saavedra, Genaro Martinez-Corral, Manuel Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Univ Valencia Dept Opt Burjassot Spain

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

three-dimensional imaging is affected by depth-induced spherical aberration (SA) when imaging deep into an optically thick sample. In this work, we evaluate the impact of SA on the performance of incoherent grating-projection structured illumination microscopy (SIM). In particular, we analyze the reduction of the contrast in the structured pattern and compare the reconstructed SIM images for different amounts of SA. In order to mitigate the impact of SA, we implement and evaluate in SIM a wavefront encoded imaging system using a square cubic (SQUBIC) phase mask, an approach shown previously to be successful in conventional microscopy.

关键词： Spherical aberration wavefront encoding fluorescence microscopy structured illumination microscopy

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Single image sectioning using spatial frequency domain imaging 24

Single image sectioning using spatial frequency domain imagi...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIV

作者： Hoffman, Zachary R. DiMarzio, Charles A. Northeastern Univ 360 Huntington Ave Boston MA 02115 USA Draper Lab 555 Technol Sq Cambridge MA 02139 USA

ISBN: (数字)9781510605824

ISBN: (纸本)9781510605817;9781510605824

Structured illumination microscopy (SIM) works well to produce depth information by removing undesired light from out of focus planes within a specimen. However, it generally requires multiple images in order to produce sectioning. We show that, under the right conditions, we can leverage Spatial Frequency Domain Imaging (SFDI) to produce both sectioning and relative depth information about a specimen using only a single image. After processing, we find that the axial resolution is comparable to that of traditional 3 phase SIM. With this technique, we are able to produce real-time videos in-vivo at 21 frames per second. Additionally, no moving parts are needed to produce sectioning at depth.

关键词： Structured Illumination optical sectioning super-resolution incoherent SFDI

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Star Test Polarimetry using Stress-Engineered Optical Elements

Star Test Polarimetry using Stress-Engineered Optical Elemen...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Ramkhalawon, Roshita Beckley, Amber M. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA

ISBN: (纸本)9780819488701

Stress-engineered optical elements have potential applications in snapshot polarimetry, in which a single irradiance image is used to measure a spatially varying polarization. In this paper, we present star test polarimetry which is a method of polarization retrieval by analyzing a single frame point spread function. A trigonally stressed window placed at the pupil plane of an imaging system is used to produce point spread functions which are then processed to extract the polarization state of the incoming beam under investigation. We outline several methods which are used to recover the Stokes parameters of a beam of unknown polarization from the irradiance distribution of its point spread function.

关键词： Polarimetry microscopy Unconventional Polarization states

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