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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是131-140 订阅

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3,3′-thiodipropanol as a refractive index matching mounting medium for fluorescence microscopy 27

3,3′-thiodipropanol as a refractive index matching mounting...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Alata Tejedo, Milvia Iris Martinez Cervantes, Juan Carlos Piazza, Valeria Ctr Invest Opt AC Loma Bosque 115 Guanajuato Mexico

ISBN: (纸本)9781510632547

In this work, several tests of the performance of 3,3'-thiodipropanol (TDP) as a mounting medium for fluorescence microscopy of biological samples were performed. Besides optical properties like the dispersion curve of TDP and the effect of the embedding medium on the fluorescence of commonly used dyes, the interaction with biological specimens, including the labeling of filamentous actin with fluorescent phalloidins, was tested: TDP showed to represent an interesting alternative to commercial mounting media. By mixing TDP with 2,2'-thiodiethanol (TDE), it was possible not only to fine tune the refractive index of the resulting solution, but to preserve the compatibility with fluorescent phalloidins.

关键词： Mounting media fluorescence microscopy filamentous actin

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three-dimensional super-resolution imaging of single molecules by spectrally selective confocal microscopy.

Three-dimensional super-resolution imaging of single molecul...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VI

作者： Brakenhoff, GJ van Oijen, AM Kohler, J Muller, M Schmidt, J Univ Amsterdam Dept Mol Cytol BioCentrum Amsterdam NL-1098 SM Amsterdam Netherlands

ISBN: (纸本)0819430757

It is shown that based on spectrally selective excitation of individual molecules in the focus of a high NA lens together with position sensitive imaging sub-resolution imaging of three-dimensional structures can be realised. The feasibility of the idea is demonstrated with NA = 0.55 optics on a model system of pentacene molecules in p-terphenal host matrix.

关键词： three-dimensional superresolution fluorescence single molecule spectrally selective cryogenic

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3D Effects in DIC images of Extended Objects

3D Effects in DIC Images of Extended Objects

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVI

作者： Sierra, Heidy DiMarzio, Charles A. Brooks, Dana H. Northeastern Univ Dept Elect & Comp Engn Boston MA 02115 USA

ISBN: (纸本)9780819474308

Phase microscopy has been widely used to acquire information about unstained transparent biological objects. These objects are well visualized by techniques such as Differential Interference Contrast (DIC) microscopy, but standard DIC systems don't provide quantitative phase information. Quantitative analysis is limited by the nonlinear relation between the intensity image and the object phase. In this work we combine nonlinearity concepts of the image formation process to model 3D DIC images, specifically the point spread function (PSF) formulation;capturing behavior of measured images along the optical axis that are not obtained when using other models. We verify our model by comparing simulated images to real measured images. This model is designed so that the reconstruction of the 3D properties of imaged specimens can be done in a simple form.

关键词： Differential Interference Contrast (DIC) thick objects coherent microscopy image formation theory

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Graphics processing unit-accelerated real-time compressive sensing spectral domain optical coherence tomography 22

Graphics processing unit-accelerated real-time compressive s...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXII

作者： Xu, Daguang Huang, Yong Kang, Jin U. Johns Hopkins Univ Dept Elect & Comp Engn Baltimore MD 21218 USA

ISBN: (纸本)9781628414202

In this paper, we systematically demonstrate two real-time CS SD OCT systems based on a conventional desktop having three GPUs. The first one takes fast Fourier transform (FFT) as the sensing technique and under-sampled linear wavenumber spectral sampling as input data, while the second one uses non-uniform fast Fourier transform (NUFFT) and under-sampled nonlinear wavenumber spectral sampling, respectively. The maximum reconstruction speed of 72k and 33.5k A-line/s were achieved for these two systems, respectively, with A-scan size 2048. It is > 100 times faster than the C++ implementation and > 400 times faster than the MATLAB implementation. Finally, we present real-time dispersion compensated image reconstruction for both systems.

关键词： Compressive sensing optical coherence tomography graphics processing unit reconstruction algorithm image processing

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Application of linear-scale differential analysis in phase correlation method of image stitching 13

Application of linear-scale differential analysis in phase c...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Bezzubik, Vitalii V. Belashenkov, Nickolai R. Losev, Sergei V. ITMO Univ Kronverksky Pr 49 St Petersburg 197101 Russia

ISBN: (纸本)9781628419474

A robust method of creation of panoramic images which does not consume much processing resources is proposed and investigated. The phase correlation (PC) method is taken as a basic one because of simplicity of its algorithm and low computing time due to application of FFT technique. Standard PC procedure is modified by preprocessing of source frames of panoramic images in spatial domain. Preprocessing comprises Linear-Scale Differential Analysis (LSDA) with sequent content-dependent thresholding of intensity gradients. Method is proved for artificially blurred and noise corrupted images. It is shown that new robust algorithm allows to increase the productivity of creation of panoramic images keeping the probability of successive stitching close to maximum even for low-quality source frames.

关键词： Digital image image stitching phase correlation linear-scale differential analysis

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Multispectral time-resolved fluorescence microscopy based on compressive acquisitions 29

Multispectral time-resolved fluorescence microscopy based on...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXix

作者： Ghezzi, Alberto Farina, Andrea Valentini, Gianluca Bassi, Andrea D'Andrea, Cosimo Politecn Milan Dipartimento Fis Piazza L da Vinci 32 I-20133 Milan Italy CNR IFN Piazza L da Vinci 32 I-20133 Milan Italy Ist Italiano Tecnol Ctr NanoSci & Technol PoliMi Via Pascoli 70-3 I-20133 Milan Italy

ISBN: (纸本)9781510648043;9781510648036

multidimensional fluorescence microscopy techniques produce dataset rich of information (space, emission spectrum and lifetime) to investigate photophysical processes in biological samples. To acquire a 4D dataset, one promising microscope design is based on the single-pixel camera scheme and on compressive sensing acquisitions, thanks to which the measurement time can be reduced. Within this framework, a computational step is required to move from the acquisition space to the pixel space and, subsequently, the analysis can be carried out exploiting the high dimensionality. In this work we present an experimental system and a fast-fit method that can produce a map of fluorophore concentrations in parallel to the measurement routine.

关键词： fluorescence microscopy compressive sensing multispectral imaging time-resolved imaging

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Near-field optical and atomic force constraints for super-resolution 3D deconvolution in far-field optical microscopy

Near-field optical and atomic force constraints for super-re...

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Conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Axelrod, N Radko, A Ben-Yosef, N Khatchatouriants, A Treinin, M Lewis, A Hebrew Univ Jerusalem Dept Appl Phys IL-91904 Jerusalem Israel

ISBN: (纸本)081943535X

We demonstrate that near-field optical and atomic force microscopy data can be used for super-resolution three-dimensional (3-D) image restoration in optical sectioning fluorescence microscopy. A crucial feature in this approach is the full integration of such data sets with digital far-field images. The scanned probe data is used to provide modalities for boundary constraints which define surface optical information and spatial domains of optical alterations in a sample with a spatial precision that has been unachievable in the past. A restoration algorithm that can use such a complex of data for 3-D image deconvolution is presented. It uses a Tikhonov-Miller regularization scheme and allows for the imposition of different types of constraints to obtain super-resolution deconvolved images. Performance was tested by using simulated 3-D imaging. An example is given of the restoration of a 3-D wide field optical image of a biological specimen in the presence of atomic force constraints.

关键词： image restoration deconvolution algorithm constraints probe microscopy NSOM super-resolution

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Single image structured illumination (SISIM) for in-vivo imaging 25

Single image structured illumination (SISIM) for in-vivo ima...

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Hoffman, Zachary R. Kivanc, Kose Di Marzio, Charles A. Northeastern Univ 360 Huntington Ave Boston MA 02115 USA Mem Sloan Kettering Canc Ctr 1275 York Ave New York NY 10021 USA

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Recently, it has been demonstrated that sectioning within a turbid media using structured illumination microscopy can be greatly enhanced through the use of a Hilbert transform algorithm. This technique, known as single image structured illumination microscopy (SISIM), provides high quality sectioning even within a strongly refracting/scattering medium, such as tissue. It is found that motion artifacts comprise a large portion of the error in in-vivo imaging. Thus, we expand on the SISIM technique by implementing an image registration algorithm which greatly mitigates motion induced artifacts. Multiple images and movies are developed to demonstrate SISIMs ability to provide real-time, in-vivo imaging of human tissue.

关键词： Structured Illumination optical sectioning thick specimen turbid media incoherent light image registration

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Structured illumination microscopy using photoswitchable fluorescent proteins

Structured illumination microscopy using photoswitchable flu...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Hirvonen, Liisa Mandula, Ondrej Wicker, Kai Heintzmann, Rainer Kings Coll London Randall Div Cell & Mol Biophys London SE1 1UL England

ISBN: (纸本)9780819470362

In fluorescence microscopy the lateral resolution is limited to about 200 nm because of diffraction. Resolution improvement by a factor of two can be achieved using structured illumination, where a fine grating is projected onto the sample, and the final image is reconstructed from a set of images taken at different grating positions. Further resolution improvement can be achieved by saturating the transitions involved in fluorescence emission. Recently discovered photoswitchable proteins undergo transitions that are saturable at low illumination intensity. Combining this concept with structured illumination, theoretically unlimited resolution can be achieved, where the smallest resolvable distance will be determined by signal-to-noise ratio. This work focuses on the use of the photoswitchable protein Dronpa with structured illumination to achieve nanometre scale resolution in fixed cells.

关键词： structured illumination nonlinear excitation photoswitching Dronpa

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A wide-field microscopy technique using a linear image sensor for obtaining 3D images

A wide-field microscopy technique using a linear image senso...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Macedo, MP Barata, AJ Fernandes, AG Correia, CM Inst Engn Coimbra Dept Phys & Math P-3030 Coimbra Portugal

ISBN: (纸本)0819456756

three-dimensional (3D) microscopy is a huge field that includes several microscopy techniques. Among these techniques the confocal microscopy is widely known and used due to its improved axial resolution or optical sectioning ability. However its high cost and image acquisition time as well as low signal-to-noise ratio are important drawbacks. Other techniques use wide-field structured illumination to get depth information from the sample. The detectors in commercial microscopes are normally of the point detector type (PMTs) in confocal and area sensors (CCDs) in widefield microscopy. Examples of microscopes that take advantage of lower cost and faster readout cycles of linear image sensors are very uncommon. The purpose of this work is to develop a low-cost microscopy technique for obtaining 3D images with lower acquisition time and higher signal-to-noise ratio (SNR) than in classical confocal microcopy. It uses a linear CMOS image sensor and specific reconstruction algorithms will be able to extract 3D information from the distribution of light intensity collected. The sensor readout circuitry has been developed and tests are currently running in a laboratory prototype in reflection mode with an epi-illuminated configuration of scanning-stage type. The light source is a commercial incandescent lamp with regulated intensity. For the development, test and optimization of the reconstruction algorithms the object is mounted on a 3-axis translation stage to scan the object in three perpendicular directions. Contrast and resolution results obtained using a resolution test slide and preliminary images of integrated circuit (IC) bonding are presented.

关键词： 3D microscopy confocal microscopy reconstruction algorithms optical sectioning linear sensor

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