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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是141-150 订阅

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Fast scanning confocal microscopy with a rotary mirror array

Fast scanning confocal microscopy with a rotary mirror array

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Chen, NG Zhu, Q Univ Connecticut Dept Elect & Comp Engn Storrs CT 06269 USA

ISBN: (纸本)0819447641

We have developed a novel confocal microscopy system, which can perform both axial and lateral scanning at an ultra high speed. A rotary mirror array (RMA) consists of 36 small mirrors deployed on the surface of a round plate with a small tilting angle and a discrete rotational symmetry. When the RMA rotates at a constant speed, the focal point is scanned inside a sample periodically and linearly. We have demonstrated a scanning rate of 2400 lines per second, which can be readily improved to more than 20000 lines per second. Our scheme can also be used in a fast scanning multi-photon microscope.

关键词： rotary mirror array fast scanning confocal microscopy optical coherence microscopy

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Phase retrieval of widefield microscopy point spread functions

Phase retrieval of widefield microscopy point spread functio...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Hanser, BM Gustafsson, MGL Agard, DA Sedat, JW Univ Calif San Francisco Grad Grp Biophys San Francisco CA 94143 USA

ISBN: (纸本)0819439398

The three-dimensional imaging properties of a light microscope are traditionally described through an intensity point spread function (PSF) or its Fourier transform, the optical transfer function (OTF). However, the imaging properties can be more compactly described by a two-dimensional generalized pupil function. Use of the pupil function allows easy modification of an observed PSF to introduce known aberrations, a task that is much more difficult when using a PSF or OTF. Unfortunately, it is not straightforward to determine the complex-valued pupil function from the measured intensity PSF, because of the lack of phase information. This is the problem of phase retrieval. Several phase retrieval algorithms have been developed for two-dimensional imaging in astronomy. We have modified one such algorithm to be appropriate for the high-aperture, non-paraxial case of high resolution light microscopy. PSFs reconstructed from phase-retrieved pupil functions, modified by calculated aberrations closely reproduce the features of measured, aberrated PSFs.

关键词： phase retrieval Point Spread Function (PSF) wide-field microscopy fluorescence microscopy Optical Transfer Function (OTF) phase diversity optical microscopy pupil function

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A phase-shifting DIC technique for measuring 3D phase objects: Experimental verification

A phase-shifting DIC technique for measuring 3D phase object...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： King, SV Cogswell, CJ Univ Colorado Dept Elect & Comp Engn Boulder CO 80309 USA

ISBN: (纸本)0819452327

Experimental verification of our previously proposed linear phase imaging technique for differential interference contrast microscopy (DIC) microscopy is presented. This technique first applies phase-shifting methods to DIC to acquire linear phase gradient images in two orthogonal directions. A special Fourier integration algorithm is then applied to the combined phase gradient images to create a single linear phase image in which intensity is proportional to phase. This overcomes the limitations of traditional DIC, which cannot accurately measure the phase (i.e. refractive index or thickness) of embedded 3D phase objects. The linear phase imaging technique is implemented using a standard DIC microscope altered to allow controlled phase shifting, a low noise CCD camera, and post-processing in Matlab. The results presented confirm the linear proportionality of intensity to phase in these images.

关键词： DIC phase phase gradient phase imaging spiral phase interference microscopy

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Measurement of the Polarimetric Response of Suspended Gallium Doped Silicon Nanowires

Measurement of the Polarimetric Response of Suspended Galliu...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Theisen, Michael J. McIntyre, Brian L. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA Univ Rochester URnano Rochester NY USA

ISBN: (纸本)9780819488701

We describe an investigation of the polarimetric properties of suspended gallium doped silicon (Si:Ga) nanowires. Wire fabrication has been done with a combined gallium implantation (using a focused ion beam) and subsequent reactive ion and wet etches. A polarimetric microscope has been built and calibrated. Measurement of the polarimetric response shows a high reflectivity and strong retardance on reflection, with some samples showing low diattenuation, in contrast to conventional wire grid polarizers.

关键词： Polarimetry microscopy Nanowires

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Agile scanning using a MEMS focus control mirror in a commercial confocal microscope

Agile scanning using a MEMS focus control mirror in a commer...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Lukes, Sarah J. Dickensheets, David L. Montana State Univ Dept Elect & Comp Engn Bozeman MT 59717 USA

ISBN: (纸本)9780819498625

We present the use of a large stroke deformable membrane mirror for focus control over a range of 123 mu m in a commercial confocal microscope at 0.5-0.64 NA. The MEMS mirror is much faster than lens or stage translation allowing synchronization to x-y scanning for agile control over the 3D shape of the surface that is scanned. We describe a novel, low-actuator-count MEMS mirror designed specifically for focus control in scanning laser microscopes and present images taken with the mirror deployed on an Olympus Fluoview FV300.

关键词： confocal microscopy deformable membrane mirror MEMS MEOMS focus control aberration compensation

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Compressive sensing spectral domain optical coherence tomography with dispersion compensation

Compressive sensing spectral domain optical coherence tomogr...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Xu, Daguang Huang, Yong Kang, Jin U. Johns Hopkins Univ Dept Elect & Comp Engn Baltimore MD 21218 USA

ISBN: (纸本)9780819498625

In this paper, we describe a novel CS method that incorporates dispersion compensation into the CS reconstruction of spectral domain OCT (SD OCT) signal. We show that A-scans with dispersion compensation can be obtained by multiplying the dispersion correcting term to the undersampled linear-in-wavenumber spectral data before the CS reconstruction. We also implemented fast CS reconstruction by taking the advantage of fast Fourier transform (FFT). The matrix-vector multiplication commonly used in the CS reconstruction is implemented by a two-step procedure. Compared to the CS reconstruction with matrix multiplication, our method can obtain dispersion compensated A-scan at least 5 times faster. Experimental results show that the proposed method can achieve high quality image with dispersion compensation.

关键词： Compressive sensing optical coherence tomography reconstruction algorithm image processing dispersion compensation

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Evaluation of the use of wavefront encoding to reduce depth-induced aberration in structured illumination microscopy 25

Evaluation of the use of wavefront encoding to reduce depth-...

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Patwary, Nurmohammed Sola-Pikabea, Jorge Doblas, Ana Saavedra, Genaro Martinez-Corral, Manuel Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Univ Valencia Dept Opt Burjassot Spain

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

three-dimensional imaging is affected by depth-induced spherical aberration (SA) when imaging deep into an optically thick sample. In this work, we evaluate the impact of SA on the performance of incoherent grating-projection structured illumination microscopy (SIM). In particular, we analyze the reduction of the contrast in the structured pattern and compare the reconstructed SIM images for different amounts of SA. In order to mitigate the impact of SA, we implement and evaluate in SIM a wavefront encoded imaging system using a square cubic (SQUBIC) phase mask, an approach shown previously to be successful in conventional microscopy.

关键词： Spherical aberration wavefront encoding fluorescence microscopy structured illumination microscopy

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Phase mask optimization for 3D parallax EDF microscopy

Phase mask optimization for 3D parallax EDF Microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Beckers, Ingeborg E. Gierlak, Michael Hoeppel, Robert Landskron, Juergen Beuth Univ Appl Sci Berlin Dept Appl Phys Med Engn D-13347 Berlin Germany

ISBN: (纸本)9780819498625

Extended depth-of-field (EDF) microscopy is a well-investigated and very simple method to obtain projection images with an extended depth of focus. Despite its advantages of being a real-time method applicable to any microscopic mode with high lateral resolution that can be simply realized by extending a commercial microscope, the lack of z-correlation is still a problem. In this work we present a combined technique of EDF and stereomicroscopy. By cross-correlation depth information is obtained. Finally, 3D images are reconstructed for best phase masks and simulation results are evaluated experimentally.

关键词： 3D imaging real time imaging extended depth of field microscopy EDF space light modulation SLM stereoscopy parallax EDFP

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Multimodal optical microscope for imaging biological samples

Multimodal optical microscope for imaging biological samples

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Townsend, DJ DiMarzio, CA Laevsky, G Rajadhvaksha, M Northeastern Univ Ctr Subsurface Sensing & Imaging Syst Boston MA 02115 USA

ISBN: (纸本)0819456756

A multimodal microscope provides a researcher with the ability to capture images with multiple sources of contrast. Previous attempts at multimodal microscopy have been limited to capturing images at different instruments and digitally registering the images empirically using features common in the specimen. Other multimodal microscopes have combined different microscopy techniques, but have been limited in their sources of contrast. We present a unique microscope which contains 5 different imaging modalities, with four different sources of contrast. Quadrature microscopy measures the phase of the electric field of the light transmitted through an optically transparent specimen, by interference with a reference beam, using a polarimetric technique to resolve the ambiguities between phase and amplitude. Differential Interference Contrast (DIC) measures the phase gradient in an optically transparent specimen by combining two spatially separated images interferometrically. Confocal reflectance measures index of refraction changes in a plane or reflection from small scatterers. Confocal fluorescence and 2-photon laser scanning microscopy, measure fluorescent signatures of a specimen. The last three of these are inherently capable of producing three-dimensional images directly, through localized probing.

关键词： multimodal microscopy quadrature confocal

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Confocal microscopy with variable-focal length microlenses

Confocal microscopy with variable-focal length microlenses

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Mac Raighne, A Yang, LS Dunbar, LA McCabe, EM Scharf, T Trinity Coll Dublin Dept Phys Dublin 2 Ireland

ISBN: (纸本)0819452327

Confocal microscopy has a unique optical sectioning property, which allows three-dimensional images at different depths. Use of a microlens array is a potential alternative to the Nipkow disk for parallel imaging with high throughput in real-time confocal microscopy. The use of variable-focal-length microlenses can provide a way to axially scan the foci electronically avoiding the inflexible mechanical movement of the lens or the sample. Here we demonstrate a combination of a variable-focal-length microlens array and a fibre optic bundle as a way to create a high throughput aperture array that would be potentially applied as confocal imaging in vivo biological specimens. The incident side of the microlens array was determined by examining the focus distribution in the axial direction. The variation of the focal length obtained by changing the voltage and corresponding focus intensity were measured through a conventional microscope. Meanwhile, the fibre bundle was characterized by coupling with either coherent or incoherent light source. We use the fibre bundle as both a multiple aperture and an image-carrying element and combine it with a microlens array to built up a confocal system.

关键词： confocal microscopy endoscopy microlenses fibre bundle

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