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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

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Efficient and versatile aberration correction through sensorless adaptive optics 29

Efficient and versatile aberration correction through sensor...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXix

作者： Hu, Qi Hailstone, Martin Booth, Martin Univ Oxford Oxford England

ISBN: (纸本)9781510648043;9781510648036

Wavefront-sensorless adaptive optics methods are often used to correct phase aberrations in optical systems and thus to improve imaging quality. However, sensorless methods have an intrinsic disadvantage of requiring multiple images that can lead to non-desirable photo-bleaching. We have proposed a machine learning assisted aberration correction method which could correct aberrations consisting of not fewer than five Zernike modes with as few as two images. We showed that our method could be used in microscopes to provide instant aberration predictions when imaging biological samples of non-specific structures. We showed that compared to conventional function fitting sensorless adaptive optics methods, the new method corrected much faster with observable advantages. This novel method has a great potential to be used in any adaptive optics equipped microscopy for efficient sensorless aberration correction for biomedical microscopy.

关键词： Aberrations adaptive optics microscopy

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A Wavelet-Based Bayesian Framework for 3D Object Segmentation in microscopy

A Wavelet-Based Bayesian Framework for 3D Object Segmentatio...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Pan, Kangyu Corrigan, David Hillebrand, Jens Ramaswami, Mani Kokaram, Anil Univ Dublin Trinity Coll Dept Elect & Elect Engn Dublin 2 Ireland Trinity Coll Dublin Inst Neurosci Dublin 2 Ireland

ISBN: (纸本)9780819488701

In confocal microscopy, target objects are labeled with fluorescent markers in the living specimen, and usually appear with irregular brightness in the observed images. Also, due to the existence of out-of-focus objects in the image, the segmentation of 3-D objects in the stack of image slices captured at different depth levels of the specimen is still heavily relied on manual analysis. In this paper, a novel Bayesian model is proposed for segmenting 3-D synaptic objects from given image stack. In order to solve the irregular brightness and out-of-focus problems, the segmentation model employs a likelihood using the luminance-invariant 'wavelet features' of image objects in the dual-tree complex wavelet domain as well as a likelihood based on the vertical intensity profile of the image stack in 3-D. Furthermore, a smoothness 'frame' prior based on the a priori knowledge of the connections of the synapses is introduced to the model for enhancing the connectivity of the synapses. As a result, our model can successfully segment the in-focus target synaptic object from a 3D image stack with irregular brightness.

关键词： image segmentation Wavelets 3D modeling 3D image processing 3D acquisition Feature extraction Confocal microscopy Binary data Discrete wavelet transforms microscopy

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Multi-wavelength phase imaging interference microscopy

Multi-wavelength phase imaging interference microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Warnasooriya, Nilanthi Kim, Myung K. Univ S Florida Dept Phys Tampa FL 33620 USA

ISBN: (纸本)0819461326

Multi-wavelength phase imaging interferography is a technique that combines phase-shifting interferometry with multiwavelength phase unwrapping. It can be used to obtain phase profile of an object without 2 pi ambiguities inherent to single wavelength phase images. In this technique, a Michelson-type interferometer is illuminated by an LED and the reference mirror is dithered for obtaining interference images at four phase quadratures, which are combined to calculate the phase of the object surface. The 2 pi discontinuities are removed by repeating the experiment using two or three LEDs at different wavelengths, which yields phase images of effective wavelength much longer than the original. The resulting image is a profile of the object surface with a height range of several microns and noise levels of 10's of nm. The technique is applied to imaging of phase profile of microscopic objects. The interferographic images using broadband source are significantly less affected by coherent noise, and there are a number of other advantages, such as lower cost and ease of operation.

关键词： interference microscopy phase shift phase measurement

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three-dimensional imaging with a quadrature tomographic microscope

Three-dimensional imaging with a quadrature tomographic micr...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Stott, JJ Bennett, RE Warner, CM DiMarzio, CA Northeastern Univ Ctr Subsurface Sensing & Imaging Syst Boston MA 02115 USA

ISBN: (纸本)0819439398

The quadrature tomographic microscope combines conventional microscopy with interferometric quadrature detection techniques to yield a simultaneous measurement of both the amplitude and phase of the electric field. This is of tremendous utility in imaging nearly-transparent objects since changes in phase can be directly attributed to changes in the object's index of refraction while the small amplitude changes provide additional structural information. In this paper we will discuss the progress we have made towards reconstructing three dimensional images from the measured two-dimensional complex electric field. BS applying an artificial curvature to the measured field and back-propagating the Gaussian beams, we are able to digitally manipulate the imaging kernel and image different focal planes.

关键词： microscopy optical quadrature tomography quantitative biology

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Novel contrast mechanism for label free super-resolution imaging 13

Novel contrast mechanism for label free super-resolution ima...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Alexandrov, Sergey A. McGrath, James Subhash, Hrebesh Boccafoschi, Francesca Giannini, Cinzia Leahy, Martin Natl Univ Ireland Sch Phys Tissue Opt & Microcirculat Imaging Grp Galway Ireland Univ Piemonte Orientale Dept Hlth Sci I-28100 Novara Italy CNR Inst Crystallog Via Amendola 122-O I-70126 Bari Italy

ISBN: (纸本)9781628419474

We present a novel super-resolution spectral encoding of spatial frequency (srSESF) approach to break the diffraction limit and dramatically improve resolution in lateral direction. The idea is to utilize additional information about the internal structure of the object to resolve features in the lateral direction. The novel contrast mechanism is realized by reconstruction and comparison of the axial spatial frequency (period) profiles at each image point to form superresolution image. As a result, small features, unresolved by conventional microscopy, can be visualized. Numerical simulation and experiments confirm the super-resolution abilities of the srSESF approach without applying any labels.

关键词： Optical microscopy label-free imaging super-resolution

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Lensfree on-chip high-resolution imaging using two-way lighting, and its limitations 13

Lensfree on-chip high-resolution imaging using two-way light...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Adachi, Yasuhiko Tamaki, Tokuhiko Motomura, Hideto Kato, Yoshihisa Panasonic Corp Adv Res Div Osaka Japan Panasonic Corp Automot & Ind Syst Co Div Engn Kyoto Japan

ISBN: (纸本)9781628419474

A high-magnification image of a biological sample can generally be obtained by an optical microscope with an objective lens, moving the image sensor with a sub-pixel shift and the subsequent image processing for super-resolution. However, to obtain a high-resolution image, a large number of images will be required for the super-resolution, and thus it is difficult to achieve real-time operation, and the field-of-view (FOV) is not sufficiently wide. The currently proposed digital holography technique places a sample on the image sensor and captures the interference fringe (hologram) to reconstruct a 3D high-resolution image in a computer. This technique ensures the features of a wide FOV, whereas the high resolution obtained by image processing cannot ensure real-time operation, because it requires recursive calculations of light propagation and adequate computer resources. To realize wide FOV and the real-time operation at the same time, we have developed a new technique: Lensfree on-chip high-resolution imaging using two-way lighting. High-resolution image is immediately obtained by image processing of the low-resolution images of the samples. This makes it possible to ensure a wide FOV, a deep depth of focus without the need for focus adjustment, and a continuously expanding operation. We also discuss the limitations of the high resolution.

关键词： Lensfree microscopy On-chip microscopy Two-way lighting

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Optically Sectioned Imaging by Oblique Plane microscopy

Optically Sectioned Imaging by Oblique Plane Microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Kumar, Sunil Lin, Ziduo Lyon, Alex R. MacLeod, Ken T. Dunsby, Chris Univ London Imperial Coll Sci Technol & Med Dept Phys Photon Grp S Kensington Campus London SW7 2AZ England Imperial Coll London Natl Heart & Lung Inst London W2 1NY England Imperial Coll London Natl Heart & Lung Inst London SW3 6LY England Imperial Coll London Dept Med Div Med London W12 0HS England

ISBN: (纸本)9780819484413

Oblique Plane microscopy (OPM) is a light sheet microscopy technique that combines oblique illumination with correction optics that tilt the focal plane of the collection system. OPM can be used to image conventionally mounted specimens on coverslips or tissue culture dishes and has low out-of-plane photobleaching and phototoxicity. No moving parts are required to achieve an optically sectioned image and so high speed optically sectioned imaging is possible. The first OPM results obtained using a high NA water immersion lens on a commercially available inverted microscope frame are presented, together with a measurement of the achievable optical resolution.

关键词： Fluorescence microscopy three-dimensional microscopy Selective Plane Illumination microscopy

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Simultaneous multiplane imaging for 3D confocal microscopy using high-speed z-scanning multiplexing 22

Simultaneous multiplane imaging for 3D confocal microscopy u...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXII

作者： Duocastella, Marti Vicidomini, Giuseppe Diaspro, Alberto Ist Italiano Tecnol Nanophys Via Morego 30 I-16163 Genoa Italy

ISBN: (纸本)9781628414202

One of the key frontiers in optical imaging is to maximize the spatial information retrieved from a sample while minimizing acquisition time. Confocal laser scanning microscopy is a powerful imaging modality that allows real-time and high-resolution acquisition of two-dimensional (2D) sections. However, in order to obtain information from three-dimensional (3D) volumes it is currently limited by a stepwise process that consists of acquiring multiple 2D sections from different focal planes by slow z-focus translation. Here, we present a novel method that enables the capture of an entire 3D sample in a single step. Our approach is based on an acoustically-driven varifocal lens integrated in a commercial confocal system that enables axial focus scanning at speeds of 140 kHz or above. Such high-speed allows for one or multiple focus sweeps on a pixel by pixel basis. By using a fast acquisition card, we can assign the photons detected at each pixel to their corresponding focal plane allowing simultaneous multiplane imaging. We exemplify this novel 3D confocal microscopy technique by imaging different biological fluorescent samples and comparing them with those obtained using traditional z-scanners. Based on these results, we find that image quality in this novel approach is similar to that obtained with traditional confocal methods, while speed is only limited by signal-to-noise-ratio. As the sensitivity of photodetectors increases and more efficient fluorescent labeling is developed, this novel 3D method can result in significant reduction in acquisition time allowing the study of new fundamental processes in science.

关键词： confocal microscopy optofluidics acousto-optics 3D imaging liquid lenses

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Optical coherence computed tomography for quantitative 3D imaging of adult zebrafish (Withdrawal Notice) 25

Optical coherence computed tomography for quantitative 3D im...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXV 2018

作者： Van Der Horst, Jelle Trull, Anna Kalkman, Jeroen Technische Univ. Delft Netherlands

“Publisher’s Note: This conference presentation, originally published on 3/21/18, was withdrawn per author request.”

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614833

“Publisher’s Note: This conference presentation, originally published on 3/21/18, was withdrawn per author request.”

关键词： Tissue optics Coherence (optics) Computed tomography Optical coherence tomography Optical imaging Refractive index Signal attenuation 3D image processing Light scattering Optical tomography

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Confocal imaging with orthogonally polarized illumination beams 13

Confocal imaging with orthogonally polarized illumination be...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Kalita, Ranjan Boruah, Bosanta R. Indian Inst Technol Guwahati Dept Phys Gauhati Assam India

ISBN: (纸本)9781628419474

In confocal microscopy the polarization of the illumination beam plays an important role in determining the orientation of the fluorescent molecules being illuminated. The efficiency of the excitation depends on the angle between the excitation electric field and the direction of the molecular dipole. In order to determine the orientation of the fluorescent molecules in the focal plane the molecules are to be excited using two mutually orthogonal electric fields. In this paper we show how a computer generated holography technique can be implemented using a ferroelectric liquid crystal spatial light modulator to conveniently obtain two images of the same target once with an X polarized illumination beam and another with a Y polarized illumination beam.

关键词： Confocal microscopy Computer generated holography Polarization Molecular orientation

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