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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是161-170 订阅

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A novel method for image denoising of fluorescence molecular imaging based on fuzzy C-Means clustering 22

A novel method for image denoising of fluorescence molecular...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXII

作者： An, Yu Liu, Jie Ye, Jinzuo Mao, Yamin Yang, Xin Jiang, Shixin Chi, Chongwei Tian, Jie Beijing Jiaotong Univ Sch Comp & Informat Technol Dept Biomed Engn Beijing 100044 Peoples R China Inst Chinese Acad Sci Key Lab Mol Imaging Beijing 100190 Peoples R China

ISBN: (纸本)9781628414202

As an important molecular imaging modality, fluorescence molecular imaging (FMI) has the advantages of high sensitivity, low cost and ease of use. By labeling the regions of interest with fluorophore, FMI can noninvasively obtain the distribution of fluorophore in-vivo. However, due to the fact that the spectrum of fluorescence is in the section of the visible light range, there are mass of autofluorescence on the surface of the bio-tissues, which is a major disturbing factor in FMI. Meanwhile, the high-level of dark current for charge-coupled device (CCD) camera and other influencing factor can also produce a lot of background noise. In this paper, a novel method for image denoising of FMI based on fuzzy C-Means clustering (FCM) is proposed, because the fluorescent signal is the major component of the fluorescence images, and the intensity of autofluorescence and other background signals is relatively lower than the fluorescence signal. First, the fluorescence image is smoothed by sliding-neighborhood operations to initially eliminate the noise. Then, the wavelet transform (WLT) is performed on the fluorescence images to obtain the major component of the fluorescent signals. After that, the FCM method is adopt to separate the major component and background of the fluorescence images. Finally, the proposed method was validated using the original data obtained by in vivo implanted fluorophore experiment, and the results show that our proposed method can effectively obtain the fluorescence signal while eliminate the background noise, which could increase the quality of fluorescence images.

关键词： image denoising fluorescence molecular imaging fuzzy C-Means clustering

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An information-theoretic approach to designing the plane spacing for multifocal plane microscopy 22

An information-theoretic approach to designing the plane spa...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXII

作者： Tahmasbi, Amir Ram, Sripad Chao, Jerry Abraham, Anish V. Ward, E. Sally Ober, Raimund J. Texas A&M Univ Dept Biomed Engn College Stn TX 77843 USA Texas A&M Hlth Sci Ctr Dept Mol & Cellular Med College Stn TX USA Univ Texas SW Med Ctr Dallas Dept Immunol Dallas TX 75390 USA Texas A&M Hlth Sci Ctr Dept Microbial Pathogenesis & Immunol College Stn TX USA

ISBN: (纸本)9781628414202

Multifocal plane microscopy (MUM) is a 3D imaging modality which enables the localization and tracking of single molecules at high spatial and temporal resolution by simultaneously imaging distinct focal planes within the sample. MUM overcomes the depth discrimination problem of conventional microscopy and allows high accuracy localization of a single molecule in 3D along the z-axis. An important question in the design of MUM experiments concerns the appropriate number of focal planes and their spacings to achieve the best possible 3D localization accuracy along the z-axis. Ideally, it is desired to obtain a 3D localization accuracy that is uniform over a large depth and has small numerical values, which guarantee that the single molecule is continuously detectable. Here, we address this concern by developing a plane spacing design strategy based on the Fisher information. In particular, we analyze the Fisher information matrix for the 3D localization problem along the z-axis and propose spacing scenarios termed the strong coupling and the weak coupling spacings, which provide appropriate 3D localization accuracies. Using these spacing scenarios, we investigate the detectability of the single molecule along the z-axis and study the effect of changing the number of focal planes on the 3D localization accuracy. We further review a software module we recently introduced, the MUMDesignTool, that helps to design the plane spacings for a MUM setup.

关键词： Cramer-Rao lower bound Fisher Information Matrix Single molecule microscopy

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three-dimensional and multidimensional microscopy: image acquisition and processing XXI

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXI

ISBN: (纸本)9780819498625

The proceedings contain 40 papers. The topics discussed include: study of self-interference incoherent digital holography for the application of retinal imaging;further developments in addressing depth-variant 3D fluorescence microscopy imaging;flash extreme ultraviolet holographic microscopy in a table top setup;compressive sensing spectral domain optical coherence tomography with dispersion compensation;the Gray Institute open microscopes applied to radiobiology and protein interaction studies;high-contrast 3D microscopic imaging of deep layers in a biological medium;analysis of phase conjugation in a turbid medium;a model for ultrasound modulated light in a turbid medium;quantifying melanin distribution using pump-probe microscopy and a 2D morphological autocorrelation transformation for melanoma diagnosis;numerical spherical aberration correction method using spatial light modulator under deep-part fluorescence observation;and wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light.

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Spectral-domain interferometry for quantitative DIC microscopy

Spectral-domain interferometry for quantitative DIC microsco...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Li, Chengshuai Zhu, Yizheng Virginia Tech Bradley Dept Elect & Comp Engn Blacksburg VA 24060 USA

ISBN: (纸本)9780819498625

A spectral-domain differential interference contrast (SD-DIC) microscopy system is presented for quantitative imaging of both reflective and transparent samples. The spectral-domain interferometry, combined with the common-path DIC geometry, provides a shot noise-limited sensitivity of 14.3pm in optical pathlength gradient measurement. The optical resolution of the system was characterized using images of a USAF resolution target. Fused silica microspheres were imaged to demonstrate the reconstruction of two-dimensional optical pathlength topography from measured gradient fields. The exquisite sensitivity of the system showed potential in quantitative imaging of sub-diffraction limit objects such as gold nanoparticles.

关键词： Differential interference contrast quantitative phase imaging spectral-domain interferometry

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Phase mask optimization for 3D parallax EDF microscopy

Phase mask optimization for 3D parallax EDF Microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Beckers, Ingeborg E. Gierlak, Michael Hoeppel, Robert Landskron, Juergen Beuth Univ Appl Sci Berlin Dept Appl Phys Med Engn D-13347 Berlin Germany

ISBN: (纸本)9780819498625

Extended depth-of-field (EDF) microscopy is a well-investigated and very simple method to obtain projection images with an extended depth of focus. Despite its advantages of being a real-time method applicable to any microscopic mode with high lateral resolution that can be simply realized by extending a commercial microscope, the lack of z-correlation is still a problem. In this work we present a combined technique of EDF and stereomicroscopy. By cross-correlation depth information is obtained. Finally, 3D images are reconstructed for best phase masks and simulation results are evaluated experimentally.

关键词： 3D imaging real time imaging extended depth of field microscopy EDF space light modulation SLM stereoscopy parallax EDFP

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High-contrast 3D microscopic imaging of deep layers in a biological medium

High-contrast 3D microscopic imaging of deep layers in a bio...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Faridian, Ahmad Pedrini, Giancarlo Osten, Wolfgang Univ Stuttgart Inst Tech Opt SCoPE Pfaffenwaldring 9 D-70569 Stuttgart Germany

ISBN: (纸本)9780819498625

Multilayer imaging of biological specimens is a demanding field of research, but scattering is one of the major obstacles in imaging the internal layers of a specimen. Although in many studies the biological object is assumed to be a weak scatterer, this condition is hardly satisfied for sub-millimeter sized organisms. The scattering medium is inhomogeneously distributed inside the specimen. Therefore, the scattering which occurs in the upper layers of a given internal layer of interest is different from the lower layers. That results in a different amount of collectable information for a specific point in the layer from each view. An opposed view dark-field digital holographic microscope (DHM) has been implemented in this work to collect the information concurrently from both views and increase the image quality. Implementing a DHM system gives the possibility to perform digital refocusing process and obtain multilayer images from each side without depth scanning of the object. The results have been presented and discussed here for a Drosophila embryo.

关键词： Digital holographic microscopy Multilayer imaging three-dimensional microscopy image enhancement image fusion

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Experimental analysis of focal fields in laser scanning fluorescence stereomicroscopy

Experimental analysis of focal fields in laser scanning fluo...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Yang, Yanlong Peng, Tong Lei, Ming Li, Runze Zhou, Xing Wu, Di Yao, Baoli Ye, Tong Chinese Acad Sci Xian Inst Opt & Precis Mech State Key Lab Transient Opt & Photon Xian Shaanxi Peoples R China Clemson Univ Ctr Optic Mat Sci & Engn Technol Dept Bioengineering Clemson SC 29631 USA

ISBN: (纸本)9780819498625

Bessel beams have been proved to have the ability to extend the depth of focus in fluorescence microscopy. But the depth discrimination was not investigated thoroughly. Following our previous work(2), we investigated focal fields of Bessel-Gauss beams at different scanning angles. We found that the central focusing lines were tilted differently at different scanning angles. This effect manifests the ability of the true perspective view in the fluorescence stereomicroscopy.

关键词： Bessel-Gauss beams extended depth of field stereomicroscopy laser scanning three-dimensional imaging volume imaging

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Detecting jaundice by using digital image processing

Detecting jaundice by using digital image processing

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Castro-Ramos, J. Toxqui-Quitl, C. Villa Manriquez, F. Orozco-Guillen, E. Padilla-Vivanco, A. Sanchez-Escobar, J. J. Inst Nacl Astrofis Opt & Electr Puebla Mexico Univ Politecn Tulancingo Tulancingo Mexico Univ Politecn Sinaloa Mazatlan Mexico Ctr Ensenanza Tecn Ind Guadalajara Jalisco Mexico

ISBN: (纸本)9780819498625

When strong Jaundice is presented, babies or adults should be subject to clinical exam like "serum bilirubin" which can cause traumas in patients. Often jaundice is presented in liver disease such as hepatitis or liver cancer. In order to avoid additional traumas we propose to detect jaundice (icterus) in newborns or adults by using a not pain method. By acquiring digital images in color, in palm, soles and forehead, we analyze RGB attributes and diffuse reflectance spectra as the parameter to characterize patients with either jaundice or not, and we correlate that parameters with the level of bilirubin. By applying support vector machine we distinguish between healthy and sick patients.

关键词： Digital image processing Diffuse Reflectance Bilirubin Support Vector Machine

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Amplitude, phase, and polarization control with a single spatial light modulator

Amplitude, phase, and polarization control with a single spa...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Theisen, Michael J. Head, Stephen T. Brown, Thomas G. Gillmer, Steven R. Ellis, Jonathan D. Univ Rochester Inst Opt Rochester NY 14627 USA Univ Rochester Dept Mech Engn Rochester NY 14627 USA

ISBN: (纸本)9780819498625

Point spread function engineering is usually accomplished by controlling the amplitude, phase and/or polarization of the pupil fields. We analyze and test an optical design for full amplitude, phase, and polarization control of the pupil fields using a single spatial light modulator. In our scheme, the beam is spatially split into four components whose relative phases provide the four degrees of freedom necessary for amplitude, phase, and polarization control.

关键词： Spatial Light Modulators Polarization Control microscopy Pupil Engineering

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Membrane-substrate separation distance assessed by normalized total internal reflection fluorescence microscopy

Membrane-substrate separation distance assessed by normalize...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Dos Santos, Marcelina Cardoso Vezy, Cyrille Jaffiol, Rodolphe Univ Technol Troyes CNRS UMR 6281 Lab Nanotechnol & Instrumentat OptInst Charles D F-10004 Troyes France

ISBN: (纸本)9780819498625

As a consequence of the recent progress in nanoscale technology, more and more sensitive methods are developed to characterize and understand the dynamic of cell membrane adhesion process. In this paper we present a new quantitative method to measure the separation distances between the membrane and the substrate. This technique is based on a normalization of Total Internal Reflection Fluorescence (TIRF) images by usual epi-illumination images. This simple method allows to achieve a nanometric axial resolution, typically 10 nm. We demonstrate the potential of our technique through the study of phospholipids membranes such as Giant Unilamellar Vesicles (GUVs), which are usual biomimetic systems to investigate membrane-substrate interactions.

关键词： Total Internal Fluorescence (TIRF) nanoscale fluorescence imaging cell adhesion Giant Unilamellar Vesicles (GUV)

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