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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是161-170 订阅

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Generation and imaging with the third harmonic

Generation and imaging with the third harmonic

引用

Conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Brakenhoff, GJ Squier, J Univ Amsterdam BioCentrum Dept Mol Cyt NL-1098 SM Amsterdam Netherlands

ISBN: (纸本)081943535X

In this paper we study the spatial distribution and wavefront characteristics of third harmonic generation in relation to some material and interface conditions over the focal region of the fundamental beam. We investigate, mostly from an experimental point of view, the implications the physics of the THG generation process has in situations where THG may be employed for -3D- imaging. Due to the non-linear character of the THG generation process it is inherently suitable for this application. For the first time images of the distribution of the THG radiation, as the interface is moved through focus, are shown. Experiments on closely spaced interfaces or bilayers confirm unambiguously the correctness of the vector model for THG generation (Ward et al. 1969) in uniform media. In view of these and other data the image formation, especially for biological objects, with THG radiation will be discussed.

关键词： THG third harmonic microscopy imaging

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Effects of a pupil filter on stimulated emission depletion microscopy

Effects of a pupil filter on stimulated emission depletion m...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Yoo, Hongki Song, Incheon Kim, Taehoon Gweon, Daegab Korea Adv Inst Sci & Technol Dept Mech Engn Lab Nano Optomechatron Taejon 305701 South Korea Hanyang Univ Dept Biomed Engn Hanyang Peoples R China

ISBN: (纸本)0819461326

Recently, stimulated emission depletion microscopy has achieved high resolution in fluorescent imaging. In this paper, we present effects of a pupil filter on the performances of stimulated emission depletion microscopy. In stimulated emission depletion microscopy, a saturated zero-centered spot is usually used to achieve a high lateral resolution. Using a half-coated phase plate, a zero-centered spot was made with a narrow and steep gap at the center. Numerical and experimental results show that by simply inserting a central obstacle as a pupil filter, it is possible to reduce the central gap of the zero-centered spot. However in order to compensate inevitable loss of light, which is blocked by the obstacle, increased laser power is required.

关键词： STED microscopy confocal microscopy high resolution pupil filter phase plate annular aperture

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Time-resolved wide-field optically sectioned fluorescence microscopy

Time-resolved wide-field optically sectioned fluorescence mi...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Dupuis, Guillaume Benabdallah, Nadia Chopinaud, Aurelien Mayet, Celine Leveque-Fort, Sandrine Univ Paris 11 CPBM Federat LUMAT FR 2764 Orsay France CNRS Orsay France Univ Paris 11 Inst Sci Mol Orsay UMR 8214 Orsay France

ISBN: (纸本)9780819493583

We present the implementation of a fast wide-field optical sectioning technique called HiLo microscopy on a fluorescence lifetime imaging microscope. HiLo microscopy is based on the fusion of two images, one with structured illumination and another with uniform illumination. Optically sectioned images are then digitally generated thanks to a fusion algorithm. HiLo images are comparable in quality with confocal images but they can be acquired faster over larger fields of view. We obtain 4-D imaging by combining HiLo optical sectioning, time-gated detection, and z-displacement. We characterize the performances of this set-up in terms of 3-D spatial resolution and time-resolved capabilities in both fixed- and live-cell imaging modes.

关键词： fluorescence microscopy fluorescence lifetime imaging microscopy (FLIM) structured illumination microscopy (HiLo) wide-field optical sectioning

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three-dimensional quantitative phase imaging: Current and future perspectives

Three-dimensional quantitative phase imaging: Current and fu...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Dragomir, Nicoleta M. Goh, Xiao Ming Roberts, Ann Univ Melbourne Sch Phys Melbourne Vic Australia

ISBN: (纸本)9780819470362

Great effort has been made in the recent past to develop new non-destructive imaging modalities for both two and three dimensional objects, based on the phase properties of a specimen. Quantitative phase tomography (QPT) is a hybrid technique that has been proposed to provide three-dimensional (3D) refractive index (RI) profiling of irregular phase objects by combining transverse phase measurements with traditional tomographic reconstruction techniques. This profiling is accomplished through measurements of sets of projections which are ultimately related to the RI values of the object's transverse cross-section. This is particularly useful for 3D refractive index determination of specimens where staining is not appropriate or for materials that cannot be stained and is essential to many applications in photonics and biotechnology. This article reviews recent developments in quantitative phase tomography as they are presently available and suggests future applications based on current research on the 3D RI. The enabling elements for 3D QPT in the context of four key areas are discussed: the effect of the refractive index of the surrounding matching fluid, spatial resolution, phase accuracy and optimal defocus. Recent progress and future perspectives related to each of these areas is presented with regard to various test objects of known optical properties.

关键词： phase imaging refractive index tomography three-dimensional microscopy

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Supercritical self-interference fluorescence microscopy for full-field membrane imaging

Supercritical self-interference fluorescence microscopy for ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Barroca, Thomas Bon, Pierre Leveque-Fort, Sandrine Fort, Emmanuel ESPCI ParisTech Inst Langevin Ctr Imageries Plasmon Appl CNRSUMR 7587 1 Rue Jussieu F-75231 Paris 05 France Univ Paris Sud CNRS CLUPS UMR 8214 F-91405 Orsay France

ISBN: (纸本)9780819493583

We present a new technique based on the self-interference of Supercritical Angle Fluorescence (SAF) emission in order to perform full-field cell membrane imaging. We show that our Point Spread Function (PSF) engineering technique allows us to obtain a 100 nm axial sectioning while conserving the original lateral resolution of the microscope. The images are acquired using an optical module that can be connected to any fluorescent microscope to simultaneously monitor in real time both the cell membrane and in-depth phenomena.

关键词： Cell membrane imaging Supercritical Angle Fluorescence (SAF) Fluorescence microscopy Dictyostelium discoideum

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LABEL-FREE CLASSIFICATION OF CELL TYPES BY IMAGING OF CELL MEMBRANE FLUCTUATIONS USING LOW-COHERENT FULL-FIELD QUANTITATIVE PHASE microscopy

LABEL-FREE CLASSIFICATION OF CELL TYPES BY IMAGING OF CELL M...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Yamauchi, Toyohiko Sugiyama, Norikazu Sakurai, Takashi Iwai, Hidenao Yamashita, Yutaka Hamamatsu Photon KK Hamakita Ku 5000 Hirakuchi Hamamatsu Shizuoka 4348601 Japan MIT Spectroscopy Lab Cambridge MA 02139 USA Hamamatsu Univ Sch Med Photon Med Res Ctr Hamamatsu Shizuoka 4313192 Japan

ISBN: (纸本)9780819479662

Cell membrane motions of living cells are quantitatively measured in nanometer resolution by low-coherent full-field quantitative phase microscopy. Our setup is based on a full-field phase shifting interference microscope with a very low-coherent light source. The reflection mode configuration and the low-coherent illumination make it possible to differentiate the weak reflection light from the cell membrane from the strong reflection from the glass substrate. Two cell populations are quantitatively assessed by the power spectral density of the cell surface motion and show different trends.

关键词： quantitative phase microscope cell imaging plasma membrane

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Performance evaluation of point-spread function engineering to reduce the impact of depth-induced aberrations on extended depth-of-field microscopy

Performance evaluation of point-spread function engineering ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Yuan, Shuai Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA

ISBN: (纸本)9780819488701

In this study, we evaluated a point-spread function (PSF) engineered using wave front encoding (WFE) and a generalized cubic phase mask (GCPM) design selected to reduce the impact of depth-induced spherical aberration (SA) on extended depth-of-field (EDOF) microscopy with high NA lenses. Mean-square-error based metrics computed from three-dimensional (3D) depth-variant WFE-PSFs with increasing amounts of SA were used to quantify the engineered PSF's sensitivity to SA and to compare it to the sensitivity of the cubic-phase mask (CPM) PSF traditionally used for EDOF microscopy. The potential performance of the engineered PSF with resilience to SA was further evaluated with simulations in which EDOF images of a 3D object were obtained by processing WFE images with and without SA. A qualitative and quantitative comparison of the EDOF images with the true object show that the WFE-PSF engineering with the selected GCPM design provides better performance in reducing the impact of SA. In addition, the GCPM-based EDOF images do not suffer from the known lateral shift of object features located away from the plane of focus encountered in traditional CPM-based EDOF images.

关键词： Extended depth-of-field microscopy point-spread function engineering wavefront encoding spherical aberration

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Multifocus imaging via polarization wavefront shaping 27

Multifocus imaging via polarization wavefront shaping

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Li, Chen Ding, Changqin Li, Minghe Rong, Jiayue Ulcickas, James Simpson, Garth J. Purdue Univ Dept Chem W Lafayette IN 47907 USA

ISBN: (纸本)9781510632547

Conventional three-dimensional (3D) images of biological samples are typically assembled from a stack of two-dimensional images acquired sequentially at different focal planes. This time-consuming manner hinders the application of 3D imaging techniques to the investigation of fast biochemical dynamics and light-sensitive biological events. The concept of multifocus imaging, which enables simultaneous acquisition of images from multiple focal planes, was introduced to achieve rapid 3D imaging. In the present study, we achieved multifocus imaging through polarization wavefront shaping via a micro-retarder array which splits the incident linearly polarized light into three beamlets that are focused to three axially-offset focal planes with 100 itm separation. Append to an existing beam-scanning microscope, this multifocus system enables rapid 3D imaging compatible with a variety of optical microscopic approaches including laser transmittance, two-photon excited fluorescence, and second harmonic generation microscopy.

关键词： Multifocus imaging beam scanning microscopy polarization wavefront shaping Jones calculus

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Fabrication and characterization of novel microsphere-embedded optical devices for enhancing microscopy resolution 25

Fabrication and characterization of novel microsphere-embedd...

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Darafsheh, Arash Washington Univ Sch Med Dept Radiat Oncol St Louis MO 63110 USA

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Microsphere-assisted imaging can be incorporated onto conventional light microscopes allowing wide-field and flourescence imaging with enhanced resolution. We demonstrated that imaging of specimens containing sub diffraction-limited features is achievable through high-index microspheres embedded in a transparent thin film placed over the specimen. We fabricated novel microsphere-embedded microscope slides composed of barium titanate glass microspheres (with diameter 10-100 lam and refractive index-4.9-2.2) embedded in a transparent polydimethylsiloxane (PDMS) elastomer layer with controllable thickness. We characterized the imaging performance of such microsphere-embedded devices in white-light microscopies, by measuring the imaging resolution, field-of-view, and magnification as a function of microsphere size. Our results inform on the design of novel optical devices, such as microsphere-embedded microscope slides for imaging applications.

关键词： Super-resolution microscopy nanoscopy microsphere barium titanate PDMS

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Super-resolution optical microscopy by using dielectric microwires 13

Super-resolution optical microscopy by using dielectric micr...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Darafsheh, Arash Wu, Gaoxiang Yang, Shu Finlay, Jarod C. Univ Penn Dept Radiat Oncol Philadelphia PA 19104 USA Univ Penn Dept Mat Sci & Engn 3231 Walnut St Philadelphia PA 19104 USA

ISBN: (纸本)9781628419474

We demonstrate that super-resolution imaging of specimens containing sub-diffraction-limited features is feasible by using dielectric microwires fabricated through capillary force lithography followed by photopatterning. As supplementary micron scale cylindrical lenses, we fabricated uniform-sized microwires with and 5 and 10 mu m diameters and refractive index similar to 1.3-1.6. The microwires are placed in contact with the specimen to collect the information of the sub-wavelength features of the specimen and transmit them to the far-field with magnification enabling imaging with two-fold resolution improvement. Potential applications of our imaging technique include biological imaging, microfluidics, and nanophotonics applications.

关键词： Super-resolution microscopy nanoscopy microwire

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