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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是171-180 订阅

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Agile scanning using a MEMS focus control mirror in a commercial confocal microscope

Agile scanning using a MEMS focus control mirror in a commer...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Lukes, Sarah J. Dickensheets, David L. Montana State Univ Dept Elect & Comp Engn Bozeman MT 59717 USA

ISBN: (纸本)9780819498625

We present the use of a large stroke deformable membrane mirror for focus control over a range of 123 mu m in a commercial confocal microscope at 0.5-0.64 NA. The MEMS mirror is much faster than lens or stage translation allowing synchronization to x-y scanning for agile control over the 3D shape of the surface that is scanned. We describe a novel, low-actuator-count MEMS mirror designed specifically for focus control in scanning laser microscopes and present images taken with the mirror deployed on an Olympus Fluoview FV300.

关键词： confocal microscopy deformable membrane mirror MEMS MEOMS focus control aberration compensation

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Compressive sensing spectral domain optical coherence tomography with dispersion compensation

Compressive sensing spectral domain optical coherence tomogr...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Xu, Daguang Huang, Yong Kang, Jin U. Johns Hopkins Univ Dept Elect & Comp Engn Baltimore MD 21218 USA

ISBN: (纸本)9780819498625

In this paper, we describe a novel CS method that incorporates dispersion compensation into the CS reconstruction of spectral domain OCT (SD OCT) signal. We show that A-scans with dispersion compensation can be obtained by multiplying the dispersion correcting term to the undersampled linear-in-wavenumber spectral data before the CS reconstruction. We also implemented fast CS reconstruction by taking the advantage of fast Fourier transform (FFT). The matrix-vector multiplication commonly used in the CS reconstruction is implemented by a two-step procedure. Compared to the CS reconstruction with matrix multiplication, our method can obtain dispersion compensated A-scan at least 5 times faster. Experimental results show that the proposed method can achieve high quality image with dispersion compensation.

关键词： Compressive sensing optical coherence tomography reconstruction algorithm image processing dispersion compensation

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Focused beam scatterometry for deep subwavelength metrology

Focused beam scatterometry for deep subwavelength metrology

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Brown, Thomas G. Alonso, Miguel A. Vella, Anthony Theisen, Michael J. Head, Stephen T. Gillmer, Steven R. Ellis, Jonathan D. Univ Rochester Inst Opt Rochester NY 14627 USA Univ Rochester Dept Mech Engn Rochester NY 14627 USA

ISBN: (纸本)9780819498625

It is known that far-field scattered light requires a priori sample information in order to reconstruct nm-scale information such as is required in semiconductor metrology. We describe an approach to scatterometry that uses unconventional polarization states in the pupil of a high NA objective lens. We call this focused beam scatterometry;we will discuss the sensitivity limits to this approach and how it relates to micro-ellipsometry as well as low-NA scatterometry.

关键词： Spatial Light Modulators Polarization Control microscopy Pupil Engineering

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Quantifying melanin distribution using pump-probe microscopy and a 2D morphological autocorrelation transformation for melanoma diagnosis

Quantifying melanin distribution using pump-probe microscopy...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Robles, Francisco E. Wilson, Jesse W. Warren, Warren S. Duke Univ Dept Chem Durham NC 27708 USA

ISBN: (纸本)9780819498625

Pump-probe microscopy is a quantitative molecular imaging technique that yields diagnostically relevant information from endogenous pigments, like melanin, by probing their ultrafast photodynamic properties. Previously, the method was applied to image thin, pigmented, cutaneous samples at different stages of melanoma, and results have shown a correlation between melanin photodynamic behavior and malignancy. Here, we add to the diagnostic power of the method by applying principles of mathematical morphology to parameterize melanins' image structure. Along with bulk melanin chemical information, results show that this method can differentiate invasive melanomas from non-invasive and benign lesions with high sensitivity and specificity (92.3% and 97.5%, respectively, with N = 53). The mathematical method and the statistical analysis are described in detail and results from cutaneous and ocular conjuctival melanocytic lesions are presented.

关键词： Melanoma microscopy Diagnostics Molecules Chemical analysis Modulation Optical parametric oscillators Statistical analysis Molecular imaging Ultrafast imaging

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Fast fluorescence holographic microscopy

Fast fluorescence holographic microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Qin, Wan Yang, Xiaoqi Li, Yingying Peng, Xiang Qu, Xinghua Yao, Hai Gao, Bruce Z. Clemson Univ Dept Bioengn Clemson SC 29634 USA Shenzhen Univ Inst Optoelect Shenzhen 518060 Peoples R China Tianjin Univ State Key Lab Precis Measuring Technol & Instrum Tianjin 300072 Peoples R China

ISBN: (纸本)9780819498625

FINCHSCOPE is a new technology of fluorescence holographic microscopy. It has been successfully applied to recording high-resolution three-dimensional fluorescence images of biological specimens without the need for scanning. In this study, we revealed and analyzed an intrinsic phenomenon, called ghost lens effect, on spatial light modulator which is the core element enabling the incoherent correlation in the FINCHSCOPE. The ghost lens effect can degrade the imaging quality by introducing multiple spherical waves with different focal lengths into the correlation and thus increasing the noise in the recorded holograms.

关键词： Fluorescence holography spatial light modulator FINCHSCOPE ghost lens effect

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Full Field Photothermal Dynamics microscopy

Full Field Photothermal Dynamics Microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Tang, Guichen Kong, Fanting Chen, Y. C. Xu, M. Fairfield Univ Dept Phys 1073 North Benson Rd Fairfield CT 06824 USA Clemson Univ Ctr Opt Mat Sci & Engn Technol Clemson SC 29625 USA Univ New York Hunter Coll Dept Phys New York NY 10065 USA

ISBN: (纸本)9780819498625

We present here a novel full field pump-probe photothermal dynamics microscopy (PTDM) which uses a numerical lock-in mechanism for capturing full field photothermal responses and is capable of imaging 2D thermal dissipation dynamics by varying the time delay between the probing and pump nano-second pulses. PTDM may find interesting applications in biology and medicine. As one example, we report PTDM imaging nuclei contained on intact hematoxylin and eosin (H&E) stained prostate cancer specimens may potentially be used to distinguish low grade and high grade prostate cancer.

关键词： photothermal microscopy dynamics full-field microscope cancer diagnosis

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Correction of image artifacts caused by refractive index gradients in scanning laser optical tomography

Correction of image artifacts caused by refractive index gra...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Antonopoulos, Georgios Christian Pscheniza, Dimitri Lorbeer, Raoul-Amadeus Heidrich, Marko Schwanke, Kristin Zweigerdt, Robert Ripken, Tammo Meyer, Heiko Laser Zentrum Hannover eV Biomed Opt Dept Hannover Germany LEBAO Hannover Med Sch Hannover Germany HTTG Dept Cardiothorac Transplantat & Vascular Surg Hannover Med Sch Hannover Germany

ISBN: (纸本)9780819498625

We present a technique for correcting image artifacts caused by refractive index distributions in Scanning Laser Optical Tomography (SLOT) and Optical Projection Tomography (OPT). Projection images can be distorted due to the presence of a refractive index distribution around the sample. We consider the special case of a refractive index distribution given by a capillary around a sample. The particular application we are interested in is in vitro imaging of cell spheroids in a glass capillary. Numerical simulations and experimental results are used to illustrate the connection between the Radon transform and the refracted projection. Thereupon we will describe a technique that transforms refracted projections to parallel ray Radon projections and thus allows artifact free reconstruction within the sample volume.

关键词： 3D fuorescence microscopy optical projection tomography scanning laser optical tomography refraction refractive index aberration correction artifact correction

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Flash extreme ultraviolet holographic microscopy in a table top setup

Flash extreme ultraviolet holographic microscopy in a table ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Monserud, Nils C. Malm, Erik B. Wachulak, Przemyslaw W. Xu, Huiwen Balakrishnan, Ganesh Chao, Weilun Anderson, Erik Marconi, Mario C. Colorado State Univ Engn Res Ctr Extreme Ultraviolet Sci & Technol Dept Elect & Comp Engn Ft Collins CO 80523 USA Mil Univ Technol Inst Optoelctron PL-00908 Warsaw Poland Univ New Mexico Ctr High Technol Mat Dept Elect & Comp Engn Albuquerque NM 87106 USA Univ Calif Berkeley Lawrence Berkeley Natl Lab Ctr Xray Opt Berkeley CA 94720 USA

ISBN: (纸本)9780819498625

We present a single-shot Fourier transform holography setup with similar to 100nm spatial resolution and 1 ns temporal resolution using a tabletop extreme ultraviolet (EUV) laser. Flash images allowed for the imaging of nano-pillars oscillating at MHz frequencies that will enable the evaluation of mechanical properties of nanoscale mechanical oscillators.

关键词： Holography Extreme ultraviolet Holograms microscopy Zone plates Fourier transforms Scanning electron microscopy Oscillators Spatial resolution Temporal resolution

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Community detection for fluorescent lifetime microscopy image segmentation

Community detection for fluorescent lifetime microscopy imag...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Hu, Dandan Sarder, Pinaki Ronhovde, Peter Achilefu, Samuel Nussinov, Zohar Washington Univ Dept Phys One Brookings DrCampus Box 1105 St Louis MO 63130 USA Washington Univ Sch Med Dept Radiol St Louis MO 63110 USA

ISBN: (纸本)9780819498625

Multiresolution community detection (CD) method has been suggested in a recent work as an efficient method for performing unsupervised segmentation of fluorescence lifetime (FLT) images of live cell images containing fluorescent molecular probes. 1 In the current paper, we further explore this method in FLT images of e x vivo tissue slices. The image processing problem is framed as identifying clusters with respective average FLTs against a background or "solvent" in FLT imaging microscopy (FLIM) images derived using NIR fluorescent dyes. We have identified significant multiresolution structures using replica correlations in these images, where such correlations are manifested by information theoretic overlaps of the independent solutions ("replicas") attained using the multiresolution CD method from different starting points. In this paper, our method is found to be more efficient than a current state-of-the-art image segmentation method based on mixture of Gaussian distributions. It offers more than 1 : 25 times diversity based on Shannon index than the latter method, in selecting clusters with distinct average FLTs in NIR FLIM images.

关键词： image segmentation Fluorescence lifetime imaging Near infrared 3D image processing Critical dimension metrology Expectation maximization algorithms microscopy image processing algorithms and systems Tissues image resolution

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Further developments in addressing depth-variant 3D fluorescence microscopy imaging

Further developments in addressing depth-variant 3D fluoresc...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Ghosh, Sreya Schaefer, Lutz Schuster, Dietwald Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Adv Imaging Methodol Consultat Kitchener ON Canada Regensburg Univ Appl Sci Regensburg Germany

ISBN: (纸本)9780819498625

three-dimensional (3D) imaging with optical sectioning microscopy uses computational methods to obtain the true fluorescence distribution by ameliorating the effect of defocus, spherical aberration and noise. Inverse algorithms improve image quality at a fraction of the cost of implementing an optical system by accurate modeling of the imaging system. Good inverse imaging algorithms need to be accurate as well as fast. Better understanding of the image formation model is vital to obtain improved restoration through model-based algorithms. Forward imaging models based on a depth-varying point-spread function (DV-PSF) leads to a substantial improvement in the resulting images because it accounts for depth-induced aberrations present in the imaging system. PSFs at every layer can be represented using their principal components. Computation of the forward imaging model using a principal component analysis (PCA) representation of the DV-PSF requires fewer convolutions than a strata based approach investigated in the past. In this paper we present a new algorithm for maximum likelihood image restoration developed based on a PCA representation of the DV-PSF and an accelerated conjugate gradient (CG) iteration scheme. Results obtained with this PCA-CG algorithm from both simulated and experimental fluorescence microscope data are discussed and compared with results obtained from a CG iteration method based on the strata model and linear interpolation of the DV-PSF. The performance of the PCA-CG algorithms is shown to be promising for practical applications.

关键词： Point spread functions Principal component analysis 3D image processing Expectation maximization algorithms 3D modeling image restoration Algorithm development Computer simulations Luminescence Reconstruction algorithms

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