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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是181-190 订阅

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Heart Synchronization for SPIM microscopy of Living Zebra Fish

Heart Synchronization for SPIM Microscopy of Living Zebra Fi...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Taylor, J. M. Saunter, C. D. Chaudhry, B. Henderson, D. J. Love, G. D. Girkin, J. M. Univ Durham Dept Phys Ctr Adv Instrumentat South Rd Durham DH1 3LE England Newcastle Univ Inst Human Genet Newcastle Upon Tyne NE1 3BZ Tyne & Wear England

We describe work on producing a selective plane illumination microscope for cardiac imaging in zebra fish embryos. The system has a novel synchronization system for imaging oscillating structures (e.g. the heart) and ... 详细信息

ISBN: (纸本)9780819484413

关键词： Optical microscopy Selective Plane Illumination Synchronization

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Fourier Analysis of Mitochondrial Distribution in Oocytes

Fourier Analysis of Mitochondrial Distribution in Oocytes

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Hollmann, Joseph L. Brooks, Dana H. Newmark, Judith A. Warner, Carol M. DiMarzio, Charles A. Northeastern Univ Dept Elect & Comp Engn Dana Res Ctr 409 360 Huntington Ave Boston MA 02115 USA Northeastern Univ Dept Biol Mugar Life Sci 134 Boston MA 02115 USA Compucyte Boston MA 02115 USA Northeastern Univ Dept Mech & Ind Engn Boston MA 02115 USA

ISBN: (纸本)9780819484413

This paper describes a novel approach to quantifying mitochondrial patterns which are typically described using the qualitative terms "diffuse" "aggregated" and are potentially key indicators for an oocyte's health and survival potential post-implantation. An oocyte was isolated in a confocal image and a coarse grid was superimposed upon it. The spatial spectrum was calculated and an aggregation factor was generated. A classifier for healthy cells was developed and verified. The aggregation factor showed a clear distinction between the healthy and unhealthy oocytes. The ultimate goal is to screen oocytes for viability preimplantation, thus improving the outcome of in vitro fertilization (IVF) treatments.

关键词： Spatial frequencies image analysis Confocal microscopy 3D image processing Algorithm development MATLAB Digital image correlation Microscopes microscopy Visualization

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Design and performance evaluation of reflection confocal microscopy using acousto-optical deflector and slit detector

Design and performance evaluation of reflection confocal mic...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Lee, SW Kang, DK Yoo, HK Gweon, DG Lee, SW Kim, KS Korea Adv Inst Sci & Technol Dept Mech Engn Taejon South Korea

ISBN: (纸本)0819452327

We describe the design and the implementation of reflection confocal scanning microscopy (CSM) using an acousto-optical deflector (AOD) for the fast horizontal scan and a galvanometer mirror (GM) for the slow vertical scan. In the beam scanning system it is important to maintain the lateral and the axial performance during scanning operation. We propose a simple method to design a scanning system using the finite ray tracing and the diffraction theory. We define a cost function which contains the effect of aberrations on the performance of microscopy. We construct the designed system and evaluate its performance. The OSLO simulation shows that the performances of CSM are not changed with deflection angle. So we conclude that the beam scanning system is properly designed. In addition, we propose an image formation method and show images obtained with the system.

关键词： confocal microscopy beam scanning system acousto-optical deflector slit detector finite ray tracing relay optics image formation

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Extended depth-of-field microscopy

Extended depth-of-field microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Smith, Christopher Botcherby, Edward Booth, Martin Juskaitis, Rimas Wilson, Tony Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

ISBN: (纸本)9780819479662

In this paper, we present details of a scanning two-photon fluorescence microscope we have built with a near-isotropic scan rate. This means that the focal spot can be scanned at high speed along any direction in the specimen, without introducing systematic aberrations. We present experimental point spread function measurements for this system using an Olympus 1.4 NA 60X oil immersion lens that demonstrates an axial range of operation greater than 70 mu m. We give details of a novel actuator device used to displace the focusing element and demonstrate axial scan responses up to 3.5 kHz. Finally, we present an application of this system in liquid crystal research to image the dynamic response of a nematic device during switching. Information about the director field at different levels in the device can be inferred from images acquired with a temporal resolution of 2.5 ms.

关键词： optical refocusing two-photon microscopy

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Restoration of high-resolution AFM images captured with broken probes

Restoration of high-resolution AFM images captured with brok...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Wang, Y. F. Corrigan, D. Forman, C. Jarvis, S. Kokaram, A. Univ Dublin Trinity Coll Dept Elect Engn Dublin 2 Ireland Univ Coll Dublin Conway Grp Nanoscale Funct Grp Dublin Ireland

ISBN: (纸本)9780819488701

A type of artefact is induced by damage of the scanning probe when the Atomic Force Microscope (AFM) captures a material surface structure with nanoscale resolution. This artefact has a dramatic form of distortion rather than the traditional blurring artefacts. Practically, it is not easy to prevent the damage of the scanning probe. However, by using natural image deblurring techniques in image processing domain, a comparatively reliable estimation of the real sample surface structure can be generated. This paper introduces a novel Hough Transform technique as well as a Bayesian deblurring algorithm to remove this type of artefact. The deblurring result is successful at removing blur artefacts in the AFM artefact images. And the details of the fibril surface topography are well preserved.

关键词： Atomic force microscopy Distortion Hough transforms Deconvolution image analysis image processing Convolution Gaussian filters image resolution Atomic force microscope

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Development of fast two-dimensional standing wave microscopy using acousto-optic deflectors

Development of fast two-dimensional standing wave microscopy...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Gliko, Olga Reddy, Duemani G. Brownell, William E. Saggau, Peter Baylor Coll Med Dept Otorhinolaryngol Houston TX 77030 USA Rice Univ Dept Bioengn Houston TX USA Baylor Coll Med Dept Neurosci Houston TX 77030 USA

ISBN: (纸本)9780819470362

A novel scheme for two-dimensional (2D) standing wave fluorescence microscopy (SWFM) using acousto-optic deflectors (AODs) is proposed. Two laser beams were coupled into an inverted microscope and focused at the back focal plane of the objective lens. The position of each of two beams at the back focal plane was controlled by a pair of AODs. This resulted in two collimated beams that interfered in the focal plane, creating a lateral periodic excitation pattern with variable spacing and orientation. The phase of the standing wave pattern was controlled by phase delay between two RF sinusoidal signals driving the AODs. Nine SW patterns of three different orientations about the optical axis and three different phases were generated. The excitation of the specimen using these patterns will result in a SWFM image with enhanced 2D lateral resolution with a nearly isotropic effective point-spread function. Rotation of the SW pattern relative to specimen and varying the SW phase do not involve any mechanical movements and are only limited by the time required for the acoustic wave to fill the aperture of AOD. The resulting total acquisition time can be as short as 100 mu s and is only further limited by speed and sensitivity of the employed CCD camera. Therefore, this 2D SWFM can provide a real time imaging of subresolution processes such as docking and fusion of synaptic vesicles. In addition, the combination of 2D SWFM with variable angle total internal reflection (TIR) can extend this scheme to fast microscopy with enhanced three-dimensional (3D) resolution.

关键词： super resolution fluorescence microscopy interference phase shift

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Fully Automated Data acquisition and Fast Data Interpretation in a Customized Multimodal Multiphoton Microscope

Fully Automated Data Acquisition and Fast Data Interpretatio...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Rahn, C. D. Meine, H. Gallinat, S. Wenck, H. Wittern, K. P. Fischer, F. Beiersdorf AG R&D Struct Res Lab Unnastr 48 D-20245 Hamburg Germany Univ Hamburg Dept Informat Cognit Syst Lab D-22527 Hamburg Germany

ISBN: (纸本)9780819479662

Examining a volunteer or patient in-vivo usually is a strictly time-constrained process. When complex (custom) multicomponent, multi-modal imaging devices are involved, an examination session might be significantly stressful for the experimenter who is in charge of instrumental control. This is especially true when many parameters, several different devices or several different software applications are to be controlled during a single session. Stress may increase the probability of suboptimal data acquisition and data loss. The experimenter should be focused on the sample, not on instrumental control. In order to ease image acquisition, we present concepts for a new intuitive and interactive software application that integrates control of all hardware components, guides the experimenter through customizable acquisition workflows, presents the recorded data ready for facilitated interpretation using state-of-the-art image processing algorithms and thus supports the experimenter in acquiring optimized and highly valuable image data. Our concept combines into a single application many functions that were previously performed by several different programs, it allows linking together the functionalities, it allows automation, and it adds several convenience and safety features.

关键词： microscopy image acquisition image analysis software automatization

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Comparison of three dimensional transfer function analysis of alternative phase imaging methods

Comparison of three dimensional transfer function analysis o...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Kou, Shan Shan Sheppard, Colin J. R. Natl Univ Singapore Div Bioengn 9 Engn Dr Singapore 117576 Singapore NUS Grad Sch Interact Sci & Engn Singapore 117597 Singapore

ISBN: (纸本)9780819465566

Phase imaging is an invaluable tool for observation of biological sample, especially for living cells, where staining might not be appropriate, or for materials that do not absorb stain. Imaging of phase distributions with high spatial resolution can be used to derive the actual thickness and refractive index variations in the specimen. The detection of very small phase variations enables the detailed structure in the specimen to be revealed. As a result, the development and utilization of various phase imaging modalities have been important aspects of microscopy research. Differential Interference Contrast (DIC) and Quantitative Phase Microcopy (QPM) are based on partially coherent light, thus enabling high-resolution imaging. However, the low coherence requirement prevents the acquisition of quantitative phase data directly. On the other hand, Digital Holography microscopy (DHM) is able to yield quantitative phase information but is compromised on resolution and cannot give full three dimensional (3D) reconstructions. In this paper, we present the 3D theoretical formalism of the above mentioned phase imaging methods with the focus on DHM. A comparative analysis here through visualization of 3D optical transfer functions gives an insight into the behaviors of these phase imaging methods.

关键词： phase imaging 3D optical transfer function

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Performance evaluation of an image estimation method based on principal component analysis (PCA) developed for quantitative depth-variant fluorescence microscopy imaging

Performance evaluation of an image estimation method based o...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Yuan, Shuai Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA

ISBN: (纸本)9780819488701

In 3D wide-field computational microscopy, the image formation process is depth variant due to the refractive index mismatch between the imaging layers. In a previous study, an image estimation method based on a principle component analysis (PCA) model for the representation of the depth varying point spread function (DV-PSF) was presented and demonstrated with noiseless simulations(1). In this study, the performance of the PCA-based DV expectation maximization algorithm (PCA-DVEM) was further evaluated with noisy simulations. Different levels of Poisson noise were used in simulated forward images of a synthetic object computed using theoretically-determined DV-PSFs approximated by the PCA approach. The noise influence on the reconstructed images obtained with PCA-DVEM was evaluated. We found that without regularization, the algorithm performs well when the signal-to-noise ratio (SNR) is 14 dB or higher. The relationship of the number of PCA components, B, to the image reconstruction performance was also investigated on both noiseless and noisy simulated data. In both cases, we found that the number of PCA components has limited effect on the image reconstruction performance for B > 1. To reduce computational complexity while maintaining image estimation performance, B = 2 is suggested for processing experimental data.

关键词： computational microscopy principle component analysis spherical aberration 3D image estimation noise analysis

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Theory and Applications of Structured Light Single Pixel Imaging 25

Theory and Applications of Structured Light Single Pixel Ima...

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Stokoe, Robert J. Stockton, Pateick A. Pezeshki, Ali Bartels, Randy A. Colorado State Univ Dept Elect & Comp Engn Ft Collins CO 80523 USA Colorado State Univ WM Keck Lab Raman Imaging Cell To Cell Commun Ft Collins CO 80523 USA Colorado State Univ Sch Biomed Engn Ft Collins CO 80523 USA

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Many single-pixel imaging techniques have been developed in recent years. Though the methods of image acquisition vary considerably, the methods share unifying features that make general analysis possible. Furthermore, the methods developed thus far are based on intuitive processes that enable simple and physically-motivated reconstruction algorithms, however, this approach may not leverage the full potential of single-pixel imaging. We present a general theoretical framework of single-pixel imaging based on frame theory, which enables general, mathematically rigorous analysis. We apply our theoretical framework to existing single-pixel imaging techniques, as well as provide a foundation for developing more-advanced methods of image acquisition and reconstruction. The proposed frame theoretic framework for single-pixel imaging results in improved noise robustness, decrease in acquisition time, and can take advantage of special properties of the specimen under study. By building on this framework, new methods of imaging with a single element detector can be developed to realize the full potential associated with single-pixel imaging.

关键词： Fluorescence microscopy interference microscopy microscopy image formation theory optical transfer functions

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