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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是201-210 订阅

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Wide-Field microscopy using Microcamera Arrays

Wide-Field Microscopy using Microcamera Arrays

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Marks, Daniel L. Youn, Seo Ho Son, Hui S. Kim, Jungsang Brady, David J. Duke Univ Dept Elect & Comp Engn Durham NC 27708 USA

ISBN: (纸本)9780819493583

A microcamera is a relay lens paired with image sensors. Microcameras are grouped into arrays to relay overlapping views of a single large surface to the sensors to form a continuous synthetic image. The imaged surface may be curved or irregular as each camera may independently be dynamically focused to a different depth. Microcamera arrays are akin to microprocessors in supercomputers in that both join individual processors by an optoelectronic routing fabric to increase capacity and performance. A microcamera may image ten or more megapixels and grouped into an array of several hundred, as has already been demonstrated by the DARPA AWARE Wide-Field program with multiscale gigapixel photography. We adapt gigapixel microcamera array architectures to wide-field microscopy of irregularly shaped surfaces to greatly increase area imaging over 1000 square millimeters at resolutions of 3 microns or better in a single snapshot. The system includes a novel relay design, a sensor electronics package, and a FPGA-based networking fabric. Biomedical applications of this include screening for skin lesions, wide-field and resolution-agile microsurgical imaging, and microscopic cytometry of millions of cells performed in situ.

关键词： Wide-field microscopy camera array image processing agile resolution

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Tomographic incoherent phase imaging, a diffraction tomography alternative for any white-light microscope

Tomographic incoherent phase imaging, a diffraction tomograp...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Bon, Pierre Aknoun, Sherazade Savatier, Julien Wattellier, Benoit Monneret, Serge Aix Marseille Univ Inst Fresnel Ecole Cent Marseille CNRSUMR7249 Domaine Univ St Jerome F-13397 Marseille France CNRS ESPCI UMR 7587 F-75005 Paris France PHSICS SA Campus Ecole Polytech F-91128 Palaiseau France

ISBN: (纸本)9780819493583

In this paper, we discuss the possibility of making tomographic reconstruction of the refractive index of a microscopic sample using a quadriwave lateral shearing interferometer, under incoherent illumination. A Z-stack is performed and the acquired incoherent elecromagnetic fields are deconvoluted before to retrieve in a quantitative manner the refractive index. The results are presented on polystyrene beads and can easily be expanded to biological samples. This technique is suitable to any white-light microscope equipped with nanometric Z-stack module.

关键词： Wavefront sensor Quantitative phase microscopy Diffraction Tomography Unlabeled biological samples

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Adapting phasor analysis for nonlinear pump-probe microscopy

Adapting phasor analysis for nonlinear pump-probe microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Robles, Francisco E. Wilson, Jesse W. Fischer, Martin C. Warren, Warren S. Duke Univ Dept Chem Durham NC 27708 USA

ISBN: (纸本)9780819493583

Phasor analysis has become a powerful tool for examining signals in fluorescence life-time microscopy (FLIM), where the analysis provides a fast, robust and intuitive means of separating different fluorescent species and mixtures thereof. In this work we adapt this analysis for pump-probe microscopy, a method that provides molecular contrast of pigmented samples by probing their excited state dynamic properties. The bipolar nature of the pump-probe signals presents important differences in the resulting phasors compared to FLIM-here, we discuss these differences and describe the behavior of bipolar signals in phasor analysis. Results show that this method is indeed able to separate multiple molecular species of interests and allows facile assessment of pigment chemistry and its distribution in samples.

关键词： microscopy Molecules Terahertz radiation Signal analyzers Statistical analysis Chemistry Fluorescence lifetime imaging Biological research Laser beam diagnostics Modulation

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Improved quantitative phase contrast in self-interference digital holographic microscopy and sensing dynamic refractive index changes of the cytoplasm using internalized microspheres as probes

Improved quantitative phase contrast in self-interference di...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Kemper, Bjoern Schubert, Robin Dartmann, Sebastian Vollmer, Angelika Ketelhut, Steffi von Bally, Gert Univ Munster Ctr Biomed Opt & Photon D-48149 Munster Germany

ISBN: (纸本)9780819493583

In self-interference digital holographic microscopy (DHM), scattering patterns that are induced by coherent laser light affect the resolution for the detection of optical path length changes. We present a simple and efficient approach for the reduction of coherent disturbances in quantitative DHM phase images by amplitude and phase modulation of the sample illumination. The performance of the method in quantitative phase imaging of living cells is illustrated. Moreover, the application of self-interference DHM for sensing of dynamic refractive index changes of adherent cells is demonstrated. Therefore, silica microspheres are used in living cells as optical probes to determine the refractive index of the cytoplasm from single quantitative phase images.

关键词： Digital holographic microscopy quantitative phase imaging cellular refractive index label-free minimally invasive live cell imaging

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Comparison of computational methods developed to address depth-variant imaging in fluorescence microscopy

Comparison of computational methods developed to address dep...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Rahman, Muhammad Mizanur Schaefer, Lutz H. Schuster, Dietwald Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Adv Imaging Mathodol Consultat Ontario ON Canada Univ Appl Sci Dept Math Regensburg Germany

ISBN: (纸本)9780819493583

In three-dimensional microscopy, the image formation process is inherently depth variant (DV) due to the refractive index mismatch between the imaging layers. In this study, we present a quantitative comparison among different image restoration techniques developed based on a depth-variant (DV) imaging model for fluorescence microscopy. The imaging models employed by these methods approximate DV imaging by either stratifying the object space (analogous to the discrete Fourier transform (DFT) "overlap-add" method) or image space (analogous to the DFT "overlap-save" method). We compare DV implementations based on maximum likelihood (ML) estimation and a previously developed expectation maximization algorithm to a ML conjugate gradient algorithm, using both these stratification approaches in order to assess their impact on the restoration methods. Simulations show that better restoration results are achieved with iterative methods implemented using the overlap-add method than with their implementation using the overlap-save method. However, the overlap-save method makes it possible to implement a non-iterative DV inverse filter that can trade off accuracy of the achieved result for computational speed. Results from a non-iterative regularized inverse filtering approach are also presented.

关键词： Depth-variant imaging 3D fluorescence microscopy spherical aberration image de-blurring

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Time-resolved wide-field optically sectioned fluorescence microscopy

Time-resolved wide-field optically sectioned fluorescence mi...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Dupuis, Guillaume Benabdallah, Nadia Chopinaud, Aurelien Mayet, Celine Leveque-Fort, Sandrine Univ Paris 11 CPBM Federat LUMAT FR 2764 Orsay France CNRS Orsay France Univ Paris 11 Inst Sci Mol Orsay UMR 8214 Orsay France

ISBN: (纸本)9780819493583

We present the implementation of a fast wide-field optical sectioning technique called HiLo microscopy on a fluorescence lifetime imaging microscope. HiLo microscopy is based on the fusion of two images, one with structured illumination and another with uniform illumination. Optically sectioned images are then digitally generated thanks to a fusion algorithm. HiLo images are comparable in quality with confocal images but they can be acquired faster over larger fields of view. We obtain 4-D imaging by combining HiLo optical sectioning, time-gated detection, and z-displacement. We characterize the performances of this set-up in terms of 3-D spatial resolution and time-resolved capabilities in both fixed- and live-cell imaging modes.

关键词： fluorescence microscopy fluorescence lifetime imaging microscopy (FLIM) structured illumination microscopy (HiLo) wide-field optical sectioning

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Dual detection confocal fluorescence microscopy: depth imaging without depth scanning

Dual detection confocal fluorescence microscopy: depth imagi...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Lee, Dong-Ryoung Kim, Young-Duk Jeong, Hyoung-Jun Lee, Jong-Min Gweon, Dae-Gab Yoo, Hongki KAIST (Korea Republic of) Hanyang Univ. (Korea Republic of)

ISBN: (纸本)9780819493583

We propose a new method for three-dimensional fluorescence imaging without depth scanning that we refer to as the dual detection confocal fluorescence microscopy (DDCFM). Compared to conventional beam-scanning confocal fluorescence microscopy, where the focal spot must be scanned either optically or mechanically to collect a three-dimensional images, DDCFM is able to obtain depth information without depth scanning. DDCFM utilizes two photo multiplier tubes (PMTs) in the confocal detection system. The emitted fluorescence is divided by the beam splitter and received by the two PMTs through pinholes with different size. Each PMT signal generates different axial response curve according to the pinhole diameter, which decides stiffness of the curve. Since the PMT signal is determined by the intensity of the fluorescent emitter and the distance from the focal point, we can acquire depth position of a fluorescent emitter by comparing two intensity signals from the PMTs. Since the depth information can be obtained by a single excitation without depth scanning, DDCFM has many advantages. The measurement time is dramatically reduced for volume imaging. Also, photo-bleaching and photo-toxicity can be minimized. The system can be easily miniaturized because no mechanical depth scan is needed. Here, we demonstrate the feasibility of the proposed method by phantom study using fluorescent beads.

关键词： Confocal fluorescence microscopy three-dimensional imaging depth scanning

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Supercritical self-interference fluorescence microscopy for full-field membrane imaging

Supercritical self-interference fluorescence microscopy for ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Barroca, Thomas Bon, Pierre Leveque-Fort, Sandrine Fort, Emmanuel ESPCI ParisTech Inst Langevin Ctr Imageries Plasmon Appl CNRSUMR 7587 1 Rue Jussieu F-75231 Paris 05 France Univ Paris Sud CNRS CLUPS UMR 8214 F-91405 Orsay France

ISBN: (纸本)9780819493583

We present a new technique based on the self-interference of Supercritical Angle Fluorescence (SAF) emission in order to perform full-field cell membrane imaging. We show that our Point Spread Function (PSF) engineering technique allows us to obtain a 100 nm axial sectioning while conserving the original lateral resolution of the microscope. The images are acquired using an optical module that can be connected to any fluorescent microscope to simultaneously monitor in real time both the cell membrane and in-depth phenomena.

关键词： Cell membrane imaging Supercritical Angle Fluorescence (SAF) Fluorescence microscopy Dictyostelium discoideum

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processing and improvements in dynamic quantitative phase microscope

Processing and improvements in dynamic quantitative phase mi...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Creath, Katherine Goldstein, Goldie 4D Technol Corp Tucson AZ 85706 USA

ISBN: (纸本)9780819493583

This paper describes recent research and development related to data processing and imaging performance for a dynamic quantitative phase imaging microscope. This microscope provides instantaneous measurements of dynamic motions within and among live cells without labels or contrast agents. It utilizes a pixelated wire grid polarizer mask in front of the camera sensor that enables simultaneous measurement of multiple interference patterns. Optical path difference (OPD) and optical thickness (OT) data are obtained from phase images. Simulated DIC (gradient) and simulated dark field (gradient magnitude) images can be directly obtained from the phase enabling simultaneous capture of brightfield, phase contrast, quantitative phase, DIC and dark field. The OT is further processed to remove background shapes, and enhance topography. This paper presents a number of different processing routines to remove background surface shape enabling quantification of changes in cell position and volume over time. Data from a number of different moving biological organisms and cell cultures are presented.

关键词： phase imaging interference microscopy polarization interferometry cellular imaging cell dynamics optical thickness measurement label-free imaging 4D microscopy quantitative phase microscopy

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Intensity-based segmentation and visualization of cells in 3D microscopic images using the GPU

Intensity-based segmentation and visualization of cells in 3...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Kang, Mi-Sun Lee, Jeong-Eom Jeon, Woong-Ki Choi, Heung-Kook Kim, Myoung-Hee Ewha Womans Univ Dept Comp Sci & Engn Seoul 120750 South Korea Inje Univ UHRC Sch Comp Engn Gyeong 621749 South Korea

ISBN: (纸本)9780819493583

3D microscopy images contain abundant astronomical data, rendering 3D microscopy image processing time-consuming and laborious on a central processing unit (CPU). To solve these problems, many people crop a region of interest (ROI) of the input image to a small size. Although this reduces cost and time, there are drawbacks at the image processing level, e. g., the selected ROI strongly depends on the user and there is a loss in original image information. To mitigate these problems, we developed a 3D microscopy image processing tool on a graphics processing unit (GPU). Our tool provides efficient and various automatic thresholding methods to achieve intensity-based segmentation of 3D microscopy images. Users can select the algorithm to be applied. Further, the image processing tool provides visualization of segmented volume data and can set the scale, transportation, etc. using a keyboard and mouse. However, the 3D objects visualized fast still need to be analyzed to obtain information for biologists. To analyze 3D microscopic images, we need quantitative data of the images. Therefore, we label the segmented 3D objects within all 3D microscopic images and obtain quantitative information on each labeled object. This information can use the classification feature. A user can select the object to be analyzed. Our tool allows the selected object to be displayed on a new window, and hence, more details of the object can be observed. Finally, we validate the effectiveness of our tool by comparing the CPU and GPU processing times by matching the specification and configuration.

关键词： GPU CUDA 3D microscopic image processing visualization

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