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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是281-290 订阅

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Quadriwave lateral shearing interferometry for quantitative phase microscopy: applications to long-duration imaging

Quadriwave lateral shearing interferometry for quantitative ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Bon, Pierre Wattellier, Benoit Monneret, Serge Marguet, Didier Aix Marseille Univ Ecole Cent Marseille CNRS Inst Fresnel Campus St Jerome F-13013 Marseille France Ecole Polytech PHASICS SA F-91128 Palaiseau France CNRS INSERM Ctr Immuno F-13288 Marseille France

ISBN: (纸本)9780819479662

Phase imaging with a high-resolution wavefront sensor is a useful setup for biological imaging. Our setup is based on a quadriwave lateral shearing interferometer mounted on a non-modified transmission white-light microscope. We propose here to study long-time duration imaging on different type of adherent cells : green monkey kidney COS7 cells, human breast epithelial MCF10A cells, and human breast cancer derived from MDA-231 cells. This study permits a enhanced visualization of the whole cell life at different levels of confluence. Post treatments on phase-shift images are proposed and become very interesting for enhanced visualization of small details and thresholding.

关键词： Wavefront sensor Quantitative phase measurements Time-lapse imaging

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Analysis of the Effects of Different Resampling Techniques for Optical Coherence Tomography

Analysis of the Effects of Different Resampling Techniques f...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Copeland, Christopher N. Ellerbee, Audrey K. Stanford Univ EL Ginzton Lab Stanford CA 94305 USA Stanford Univ Dept Elect Engn Stanford CA 94305 USA

ISBN: (纸本)9780819488701

Fourier domain optical coherence tomography (FD-OCT) uses interferometry and spatially coherent, polychromatic light to acquire cross-sectional images of scattering media such as biological tissue. Phase-sensitive derivatives of FD-OCT, such as spectral domain phase microscopy (SDPM), can perform quantitative phase imaging of cellular dynamics with sub-Angstrom sensitivity. FD-OCT and SDPM images are generated by taking the Fourier Transform of the raw spectral interferogram;the accuracy of the reconstruction depends on the choice of processing algorithms used, system noise, calibration and quantization errors. To decrease the processing time, the Fast Fourier transform (FFT) algorithm can be applied when the data are evenly sampled in wavenumber. Unfortunately, most OCT systems are designed for constant wavelength sampling, not constant wavenumber sampling. While there is general agreement on the qualitative superiority of some resampling methods over others, to the best of our knowledge there has been no study that compares these methods for OCT phase data. We examine the effects of various resampling techniques on simulated phase and amplitude OCT data. Given the trend towards high-speed imaging with OCT, the choice of resampling methods is critical, as one need balance processing time and image accuracy - not merely quality - when analyzing medical image data.

关键词： optical coherence tomography low coherence interferometry phase retrieval microscopy

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Wave-optical calibration of a light-field fluorescence microscopy 26

Wave-optical calibration of a light-field fluorescence micro...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXVI

作者： Pribosek, J. Steinbrener, J. Baumgart, M. Bereczki, T. Harms, K. Tortschanoff, A. Kenda, A. Carinthian Tech Res Europastr 12 A-9524 Villach Austria Mol Devicess Lagerhausstr 45 A-5071 Wals Austria

ISBN: (纸本)9781510624092

In light-field microscopy, a single point emitter gives rise to a complex diffraction pattern, which varies with the position of the emitter in object space. In order to use deconvolution-based wave-optical reconstruction schemes for light-field imaging systems, established methods rely on theoretical estimation of such diffraction patterns. In this paper we propose a novel method for direct experimental estimation of the light-field point spread function. Our approach relies on a modified reversed micro-Hartmann test to acquire a composite light-field point spread function of several thousand point emitters in the object plane simultaneously. By using fiducial markers and a custom image processing algorithm we separate the contributions of individual point emitters directly in raw light-field images and allow the construction of the forward imaging process without any prior assumption about the optical system required. The constructed forward imaging model can finally be applied in the 3D-deconvolution based wave-optical reconstruction scheme.

关键词： light-field microscopy light-field point spread function wave-optical calibration fluorescence imaging

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Snapshot Spectral Imaging System

Snapshot Spectral Imaging System

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Arnold, Thomas De Biasio, Martin McGunnigle, Gerald Leitner, Raimund Ctr Carinthian Tech Res Villach Austria

ISBN: (纸本)9780819479662

Spectral imaging is the combination of spectroscopy and imaging. These fields are well developed and are used intensively in many application fields including industry and the life sciences. The classical approach to acquire hyper-spectral data is to sequentially scan a sample in space or wavelength. These acquisition methods are time consuming because only two spatial dimensions, or one spatial and the spectral dimension, can be acquired simultaneously. With a computed tomography imaging spectrometer (CTIS) it is possible to acquire two spatial dimensions and a spectral dimension during a single integration time, without scanning either spatial or spectral dimensions. This makes it possible to acquire dynamic image scenes without spatial registration of the hyper-spectral data. This is advantageous compared to tunable filter based systems which need sophisticated image registration techniques. While tunable filters provide full spatial and spectral resolution, for CTIS systems there is always a tradeoff between spatial and spectral resolution as the spatial and spectral information corresponding to an image cube is squeezed onto a 2D image. The presented CTIS system uses a spectral-dispersion element to project the spectral and spatial image information onto a 2D CCD camera array. The system presented in this paper is designed for a microscopy application for the analysis of fixed specimens in pathology and cytogenetics, cell imaging and material analysis. However, the CTIS approach is not limited to microscopy applications, thus it would be possible to implement it in a hand-held device for e. g. real-time, intra-surgery tissue classification.

关键词： spectral imaging CTIS computed tomography microscopy snapshot

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Development of micro-optical projection tomography for 3D analysis of single cells

Development of micro-optical projection tomography for 3D an...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Fauver, M Seibel, EJ Rahn, JR Patten, FW Nelson, AC Univ Washington Coll Engn Human Interface Technol Lab Seattle WA 98195 USA

ISBN: (纸本)0819452327

The Micro-Optical Projection Tomographic Microscope (muOPTM) is an instrument that is being developed for three-dimensional (3D) imaging of cells and subcellular components. The target application for the muOPTM is the early detection of lung cancer by revealing the complex 3D information about chromatin redistribution in the nucleus. The muOPTM employs a scanning objective lens (100x, N.A.=1.25) to create an extended depth-of-field image, similar to a shadowgram or projection, that we call a pseudo-projection. A large number of pseudoprojections (90+) are acquired, from which a tomographically reconstructed 3D image is computed using a filtered backprojection algorithm. The prototype muOPTM uses a single objective lens, so the object (cell) must be rotated for each new pseudo-projection. A custom microtube stage minimizes the lateral and axial motion of the sample tube during scanning and rotation so that registration between successive pseudo-projections is maintained. image processing methods are used to correct any remaining registration errors. The media inside and outside the tube are refractive index-matched to each other and to the tube (Deltan(avg) < 0.02). The index-matched materials are pressed between two flat parallel windows, providing a nearly distortion-free image. Custom phantoms using microspheres have been constructed and images of these 3D test targets acquired. The minimum resolvable feature size is better than 3 microns. The first 3D image of a cell using muOPTM is also shown.

关键词： optical tomography optical projection tomography three-dimensional imaging computed tomography microscopy depth of field cancer screening

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acquisition of multiple image stacks with a confocal laser scanning microscope

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Zuschratter, W Steffen, T Braun, K Herzog, A Michaelis, B Scheich, H Fed Inst Neurobiol D-39118 Magdeburg Germany

ISBN: (纸本)0819427004

image acquisition at high magnification is inevitably correlated with a limited view over the entire tissue section. To overcome this limitation we designed software for multiple image-stack acquisition (3D-MISA) in confocal laser scanning microscopy (CLSM). The system consists of a 4 channel Leica CLSM equipped with a high resolution z-scanning stage (Leica Lasertechnik) mounted on a xy-motorized stage (Marzhauser). The 3D-MISA software is implemented into the microscope scanning software (Scanware, Leica Lasertechnik) and uses the microscope settings (magnification, zoom) for the movements of the xy-stage. It allows storage and recall of 70 xyz-positions and the automatic 3D-scanning of image arrays between selected xyz-coordinates. The number of images within one array is limited only by the amount of disk space or memory available. Although for most applications the accuracy of the xy-scanning stage is sufficient for a precise alignment of tiled views, the software provides the possibility of an adjustable overlap between two image stacks by shifting the moving steps of the xy-scanning stage. After scanning a tiled image gallery of the extended focus-images of each channel will be displayed on a graphic monitor. In addition, a tiled image gallery of individual focal planes can be created. In summary, the 3D-MISA allows 3D-image acquisition of coherent regions in combination with high resolution of single images.

关键词： multi-image-stacks confocal laserscanning microscopy 3D image acquisition

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Programmable array microsope employing two ferroelectric liquid crystal spatial light modulators

Programmable array microsope employing two ferroelectric liq...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Taylor, CM McCabe, EM Univ Dublin Trinity Coll Dept Phys Dublin 2 Ireland

ISBN: (纸本)0819439398

We present a programmable array microscope (PAM) which uses a pair of ferroelectric liquid crystal spatial light modulators (FLC SLMs). The system is similar to a confocal imaging system called a direct-view microscope (DVM), in which scanned aperture arrays are used to obtain real-time confocal images. The PAM, unlike the DVM, allows arrays to be scanned electronically rather than mechanically. In our system, one SLM is placed in the source plane of a conventional microscope system;the other is placed in the detector plane. Confocal aperture arrays are displayed and scanned synchronously on the SLMs and confocal imaging results. The resolution is improved when compared to a similar previously presented system which employed a single SLR I. We present axial resolution measurements for a variety of array dimensions and investigate the use of aperture correlation techniques to improve the light throughput in the devices.

关键词： programmable array microscopy ferroelectric liquid crystal spatial light modulator confocal microscope direct-view microscopy

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three-dimensional Fluorescence Imaging with an Automated Light-field Microscope 26

Three-dimensional Fluorescence Imaging with an Automated Lig...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXVI

作者： Steinbrener, J. Pribosek, J. Baumgart, M. Bereczki, T. Harms, K. Ripper, D. Tortschanoff, A. Kenda, A. CTR Carinthian Tech Res AG Europastr 12 A-9524 Villach Austria IMC Fachhsch Krems GmbH Piaristengasse 1 A-3500 Krems Austria Mol Devices Austria GmbH Lagerhausstr 45 A-5071 Salzburg Austria

ISBN: (纸本)9781510624092

In addition to the two-dimensional intensity distribution in the image plane, light field microscopes capture information about the angle of the incident radiation. This information can be used to extract depth information about the object, calculate all-in-focus images and perform three-dimensional reconstructions from a single exposure. In combination with automated microscopy setups, this makes the technique a promising tool for high-throughput, three-dimensional cell assay evaluation which could substantially improve drug development and screening. To this end, we have developed a novel generalized calibration and three-dimensional reconstruction scheme for a light-field fluorescence microscope setup. The scheme can handle Keplerian and Galilean light field camera configurations added to infinity corrected microscopes configured to be telecentric as well as non-telecentric or hypercentric. The latter provides a significant advantage over the state of the art as it allows for an application specific optimization of lateral and axial resolution, field-of-view, and depth-of-focus. The reconstruction itself is performed iteratively using an expectation maximization algorithm. Super-resolved reconstructions can be achieved by including experimentally measured point-spread-functions. To reduce the required computational power, sparsity and periodicity of the system matrix relating object space to light field space is exploited. This is particularly challenging for the non-telecentric cases, where the voxel size of the reconstructed object space depends on the axial coordinate. We provide details on the experimental setup and the reconstruction algorithm, and present results on the experimental verification of theoretical performance parameters as well as successful reconstructions of fluorescent beads and three-dimensional cell spheroids.

关键词： Light-field microscopy 3D fluorescence imaging Reconstruction algorithms

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Development of cellular resolution Gabor-domain optical coherence microscopy for biomedical applications 22

Development of cellular resolution Gabor-domain optical cohe...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXII

作者： Tankam, Patrice Huang, Jinxin Santhanam, Anand P. Won, Jungeun Canavesi, Cristina Rolland, Jannick P. Univ Rochester Inst Opt Rochester NY 14627 USA Univ Rochester Ctr Visual Sci Rochester NY 14642 USA Univ Calif Los Angeles Dept Radiat Oncol Los Angeles CA 90095 USA Univ Rochester Dept Biomed Engn Rochester NY 14627 USA LighTopTech Corp West Henrietta NY 14586 USA

ISBN: (纸本)9781628414202

We have developed a cellular resolution imaging modality, Gabor-Domain Optical Coherence microscopy, which combines the high lateral resolution of confocal microscopy with the high sectioning capability of optical coherence tomography to image deep layers in tissues with high-contrast and volumetric resolution of 2 mu m. A novelty of the custom microscope is the biomimetics that incorporates a liquid lens, as in whales's eyes, for robust and rapid acquisition of volumetric imaging of deep layers in tissue down to 2 mm, thus overcoming the tradeoff between lateral resolution and depth of focus. The system incorporates a handheld scanning optical imaging head and fits on a movable cart that offers the flexibility in different biomedical applications and clinical settings, including ophthalmology. In the later, the microscope has successfully revealed micro-structures within the cornea and in particular the endothelial cells micro-environment, an important step in understanding the mechanisms of Fuchs' dystrophy, a leading cause of the loss of corneal transparency. Also, the system was able to provide high definition of the edge of soft contact lenses, which is important for the fitting of the lens and the comfort of the patient. Overall, the imaging modality provides the opportunity to observe the three-dimensional features of different structures with micrometer resolution, which opens a wide range of future applications.

关键词： Optical coherence tomography imaging system image processing GPU parallel computing biomedical optics soft contact lens metrology corneal imaging

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Assessment of robust reconstruction algorithms for compressive sensing spectral-domain optical coherence tomography

Assessment of robust reconstruction algorithms for compressi...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Xu, Daguang Huang, Yong Kang, Jin U. Johns Hopkins Univ Dept Elect & Comp Engn Baltimore MD 21218 USA

ISBN: (纸本)9780819493583

In this paper, we performed an in-depth assessment of current state-of-the-art compressive sensing (CS) reconstruction algorithms, including YALL1, CSALSA, NESTA, SPGL1, TwIST and SpaRSA for use in spectral domain optical coherence tomography (SD-OCT). A brief description of mentioned algorithms and criterion in assessing performance between constraint and unconstraint algorithms are presented. The performance of all algorithms is initially assessed using a set of artificial noiseless A-scan signals with different spatial-domain dynamic range. Reconstruction error, computation time, noise tolerance and reliability of each algorithm are used as key metrics. A fair speed comparison is then implemented. Finally, computation time, SNR and local contrast of the algorithms are evaluated on real OCT B-scan data. Our results show that SPGL1 and YALL1 have moderately better performance.

关键词： Compressive sensing Optical coherence tomography Reconstruction algorithm image processing

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