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Conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Akiba, M Chan, KP Tanno, N Japan Sci & Technol Corp Reg Joint Res Project Yamagata 9902473 Japan

ISBN: (纸本)0819443603

En-face optical coherence tomography (OCT) employing parallel heterodyne detection technique is demonstrated for three-dimensional microscopy. To enable the use of commercially available CCD cameras as two-dimensional heterodyne detector arrays in OCT imaging, a frequency synchronous detection method is Employed. Depth-resolved, full-field images are acquired at 30 Hz video-rate without lateral scanning.

关键词： three-dimensional microscopy optical coherence tomography frequency synchronous detection

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three-dimensional microscopy through white light interferometry

Three-dimensional microscopy through white light interferome...

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Conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Laude, B De Martino, A Drévillon, B Benattar, L Schwartz, L Ecole Polytech CNRS UMR 7647 Lab Phys Interfaces & Couches Minces F-91128 Palaiseau France

ISBN: (纸本)0819443603

A very simple OCT system has been developed, based on a Linnik interferometric microscope with its reference mirror mounted on a piezoelectric translator. The geometrical extension of the optics allows efficient illumination of this device with a low power (3 W) light bulb, yielding full field interferometric images at 50 Hz acquisition rate with a fast CCD camera. Due to the very broad spectral width of the light source and camera response, a longitudinal resolution of 1.5 mum is achieved. Tomographic images of cell smears are shown.

关键词： Optical Coherence Tomography microscopy biomedical optical instrumentation

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Point spread functions with extended depth of focus

Point spread functions with extended depth of focus

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Conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Wilson, T Neil, MAA Massoumian, F Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

ISBN: (纸本)0819443603

The use of an annular pupil plane filter may be used to increase the depth of focus of an objective lens without significant deterioration of the lateral resolution. However this approach is very inefficient since most of the illumination light is blocked by the annular filter. We describe a method which uses a diffractive optical element to increase significantly the depth of focus but with dramatically increased light efficiency.

关键词： Axicons Point spread functions Optical components Binary data Optical filters Objectives Beam shaping Diffractive optical elements image filtering Roads

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Scanning aperture polarized light microscope: observation of small calcite crystals using oblique illumination

Scanning aperture polarized light microscope: observation of...

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Conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Shribak, M Oldenbourg, R Marine Biol Lab Woods Hole MA 02543 USA

ISBN: (纸本)0819443603

The article presents a new design of a polarization microscope with a scanning liquid crystal aperture. The scanning device is based on the earlier reported Pol-Scope technique and includes a liquid crystal universal compensator. It is mounted in the front focal plane of the high numerical aperture condenser lens on the microsccope. By occluding different portions of the aperture, an oblique beam of variable tilt angle and azimuth is created for illuminating the specimen. Birefringence measurements are recorded for different mask configurations and results are evaluated to determine the retardance magnitude, azimuth and direction of optic axis of the specimen. We report measurements using small calcite crystals that confirm our theoretical predictions.

关键词： polarization microscopy conoscopy birefringence crystal optic

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Source coherence and optical sectioning strength in direct-view microscopy

Source coherence and optical sectioning strength in direct-v...

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Conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Taylor, CM Yang, L McCabe, EM Univ Dublin Trinity Coll Dept Phys Dublin 2 Ireland

ISBN: (纸本)0819443603

The direct-view microscope is a confocal microscope which allows faster image acquisition rates than typical confocal scanning optical microscopes through the use of a pinhole array rather than the usual single pinhole. We present a theoretical investigation of the effects of source coherence on optical sectioning in direct-view microscopy. As a first step we present an equation which describes the optical sectioning strength of a coherent source brightfield DVM employing an infinite pinhole array. By simulation of both this and the finite array equation which show the existence of certain 'principal' sidelobes which are likely to represent the most problematic artifact of coherent source imaging. By further analysis of the infinite Arran, equation, we arrive at an expression which describes the defocus positions where the principal sidelobes occur. Finally, we move on to show how rectangular arrays are predicted, by the infinite array equation, to outperform square arrays and we show examples of this.

关键词： optical sectioning source coherence Real-Time imaging direct-niew microscopy Confocal microscopy

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microscopy environment for quantitative spatial and temporal analysis of multicellular interactions

Microscopy environment for quantitative spatial and temporal...

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Conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Sudar, D Parvin, B Callahan, DE Schwarz, RI Knowles, DW de Solorzano, CO Barcellos-Hoff, MH Lawrence Berkeley Lab Div Life Sci Berkeley CA 94720 USA

ISBN: (纸本)0819443603

Quantitative analysis of spatial and temporal concurrent responses of multiple markers in 3-dimensional cell cultures is hampered by the routine mode of sequential image acquisition, measurement and analysis of specific targets. A system was developed for detailed analysis of multi-dimensional, time-sequence responses and in order to relate features in novel and meaningful ways that will further our understanding of basic biology. Optical sectioning of the 3-dimensional structures is achieved with structured light illumination using the Wilson grating as described by Lanni. The automated microscopy system can image multicellular structures and track dynamic events, and is equipped for simultaneous/ sequential imaging of multiple fluorescent markers. Computer-controlled perfusion of external stimuli into the culture system allows (i) real-time observations of multiple cellular responses and (ii) automatic and intelligent adjustment of experimental parameters. This creates a feedback loop in real-time that directs desired responses in a given experiment. On-line image analysis routines provide cell-by-cell measurement results through segmentation and feature extraction (i.e. intensity, localization, etc.), and quantitation of meta-features such as dynamic responses of cells or correlations between different cells. Off-line image and data analysis is used to derive models of the processes involved, which will deepen the understanding of the basic biology.

关键词： live-cell microscopy 3-dimensional imaging real-time feedback image analysis image bioinformatics

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Application of phase retrieved pupil functions in wide-field fluorescence microscopy

Application of phase retrieved pupil functions in wide-field...

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Conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Hanser, BM Gustafsson, MGL Agard, DA Sedat, JW Univ Calif San Francisco Grad Grp Biophys San Francisco CA 94143 USA

ISBN: (纸本)0819443603

multidimensional fluorescence microscopy data are often collected under imaging conditions that cause aberrations. For proper deconvolution of such data, one needs to generate a Point Spread Function (PSF) that includes the aberrations. Even if it is possible to model the imaging aberrations theoretically, there remains the problem of modifying the PSF accordingly. Measured PSFs are difficult to modify, and theoretical PSFs lack lens- and system-specific features. Phase retrieval approaches can be used to produce a compact and modifiable description of a microscope system, including its measured, system-specific performance. These pupil functions can be used in a depth-dependent deconvolution to correct for geometric distortions and improve restoration of relative intensities compared to spatially invariant deconvolution using unaberrated PSFs.

关键词： phase retrieval Point Spread Function (PSF) wide-field microscopy fluorescence microscopy Optical Transfer Function (OTF) phase diversity optical microscopy pupil function depth aberrations spherical aberrations

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High-resolution three-dimensional imaging using multiple nanometric probes

High-resolution three-dimensional imaging using multiple nan...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Dubois, A Moneron, G Lecaque, R Lequeux, F Boccara, AC Ecole Super Phys & Chim Ind Ville Paris CNRS UPR A0005 Lab Opt Phys F-75231 Paris 5 France

We present a new imaging modality using nanometric beads as multiple local probes. The positions of the beads are determined in three dimensions using white-light interference microscopy, by over-sampling and fitting ... 详细信息

ISBN: (纸本)0819447641

关键词： interference microscopy three-dimensional microscopy scanning probe microscopy

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High resolution 3D-reconstruction from serial sections - Microscope instrumentation, software design and its implementations

High resolution 3D-reconstruction from serial sections - Mic...

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Conference on 3-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Verbeek, FJ Boon, PJ Netherlands Inst Dev Biol Hubrecht Lab NL-3584 CT Utrecht Netherlands

ISBN: (纸本)0819443603

This paper describes a set-up for high-resolution imaging with a conventional microscope that allows producing a 3D-image set from which a 3D model can be derived. The 3D-image set, is de facto, a gray value voxel model. In order to obtain such 3D image set a good administration needs to be maintained at acquisition and moreover, the acquisition must be realized in two steps. High-resolution images are built of image tiles and sophisticated algorithms are required to build a coherent image from the tiles.

关键词： image processing high-resolution image image tile image mosaic 3D reconstruction 3D image bioinformatics

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Data compression in microscopy: a comparative study

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Proceedings of SPIE - The International Society for Optical Engineering 1999年 3605卷 158-168页

作者： Kriete, Andres Klein, Norbert Berger, Lars C. Inst of Anatomy and Cell Biology Giessen Germany

ISBN: (纸本)0819430757;9780819430755

image compression in microscopy is a valuable technique, in particular if applied to multidimensional data. Information-preserving and competitive information-losing compression is applied to microscopical data and the resulting image quality is evaluated on a quantitative basis both in the spatial and frequency domain. Included are image data featuring different signal-to-noise ratios, but also voxel data for volume representations as well as graphical data for surface representations. The effect of compression on 3-D visualization and image management with data bases is included.

关键词： image compression

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