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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是571-580 订阅

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three-dimensional reconstruction by focus sensing from conventional 2D image slices

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Nahm, KB Shin, ES Ryoo, SM Hallym Univ Dept Phys Chunchon 200702 Kangwon South Korea

ISBN: (纸本)0819427004

We introduce an optical means of reconstructing the topology of rough objects observed under the microscope or at a relatively high magnification. First, 2D images were obtained under the conventional optical microscope or under the macroscopic imaging system, focused on differing height of the sample. The depth information was extracted by sensing the focus of each pixel of the conventional image slice. Each sample height associated with the best "focus figure" for each pixel was marked and were later utilized to generate the three dimensional coordinate map.

关键词： surface reconstruction microscopy focus sensing image slice

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microscopy using an interference pattern as illumination source

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Juskaitis, R Neil, MAA Wilson, T Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

ISBN: (纸本)0819427004

We describe a method of obtaining optically sectioned fluorescence images in a widefield conventional microscope by interfering two beams on an object so as to illuminate it with a single spatial frequency fringe pattern. images taken at three spatial positions of the fringe pattern are processed in real time to produce optically sectioned images which are substantially similar to those obtained with confocal microscopes.

关键词： 3D microscopy confocal microscopy fluorescence microscopy

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Polarised light microscopy

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Torok, P Higdon, PD Wilson, T Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

ISBN: (纸本)0819427004

In this work we discuss the role of polarisation in confocal microscopy. The effect of the optical system on light polarisation is analysed in the absence of a specimen. We also present numerical results on confocal microscopes imaging small dielectric scatterers and suggest an optimum pinhole size for crossed linear polars. Finally, imaging of large spherical scatterers and contrast optimisation of the optical system is discussed.

关键词： confocal microscopy polarised light vectorial theory high aperture theory

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Comparing methods for 3D microscopy

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： McNally, JG Markham, J Conchello, JA Washington Univ Inst Biomed Comp St Louis MO 63110 USA

ISBN: (纸本)0819427004

In previous studies, we examined three-dimensional images of a latex bead containing a surface layer of fluorescence whose thickness was determined by physical sectioning. Confocal images of the bead from six different microscopes all exhibited a significantly thicker fluorescent shell than actually present. In contrast, deconvolved wide-field images of the bead produced an image with the correct shell thickness. We have now repeated some of these studies using a new latex bead containing a much thinner layer of surface fluorescence. In contrast to earlier studies, confocal images of this bead appear to show an accurate thickness for the fluorescent shell. These particular confocal microscopes (a Biorad 1024 and a Zeiss 510) also yielded essentially aberration free images, which was not the case for some of the earlier microscopes tested. In these recent studies however, deconvolution of the bead appeared less robust than earlier. Of three independent wide-held images of the bead, only one yielded a substantially artifact-free image upon deconvolution.

关键词： 3D microscopy comparison confocal wide held deconvolution

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Transmission confocal microscopy: making it a reality

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Arnison, MR Fekete, PW Serrano, M Nguyen, PTA Romagnoli, R Guan, L O'Byrne, JW Cogswell, CJ Univ Sydney Sch Phys Dept Phys Opt Sydney NSW 2006 Australia

ISBN: (纸本)0819427004

Imaging thick specimens in 3D transmission confocal modes presents two key problems. The first problem is variable aberrations introduced by changes in refractive index. The second problem is revealed when visualising acquired data, where thick 3D datasets are difficult to interpret. In this paper we present our emerging solutions to these problems. Aberrations can be classified as simple tip-tilt deflection of the beam, or more complicated higher order aberrations. We discuss our results which demonstrate successful on-the-fly detection and correction for tip-tilt For detecting higher order aberrations, we have chosen to investigate the wavefront curvature sensing technique. The second problem of rendering thick 3D datasets can be solved by extracting features of interest from the background. Simple intensity threshholding is not sufficient for complex biological specimens, and image processing in only two dimensions neglects any three dimensional structure. Use of Kohonen's self-organising map neural network in 3D results in clear segmentation of features for sample chromosome specimens.

关键词： 3D microscopy confocal transmission DIC adaptive optics neural network image processing

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three-dimensional limited-angle microtomography of blood cells: experimental results

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Levin, GG Vishnyakov, GN Zakarian, CS Likhachov, AV Pickalov, VV Kozinets, GI Novoderzhkina, JK Streletskaya, EA Russian Res Inst Optophys Measurements Moscow 119361 Russia

ISBN: (纸本)0819427004

Tomographic measurements of the 3D refractive index spatial distribution within optically transparent phase samples with computerized interferometric microscopes are proposed. Phase shifting interferometric microtomography applications for the 3D image reconstruction of the blood cells (lymphocytes) are represented. The immersion 100x, N.A.=1.25 objective was used to increase the spatial resolution and observation angle range to 90 degree. ART, combined ART and iterative Gerchberg-Papoulis 3D algorithm were used for the tomogram reconstruction. To determine the accuracy and spatial resolution of the blood cells image reconstruction by means of the interferometric microtomographic method the numerical simulations were implemented.

关键词： microtomography interferometric microscope refractive index living cells and lymphocyte

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Improved optical method for measuring of AFM cantilever deflection

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Baibyrin, VB Konnov, NP Volkov, UP Saratov Tech Univ Saratov 410016 Russia

ISBN: (纸本)0819427004

In our lab have been developed an atomic force microscope (AFIM) for biological and technical applications with improved optical method for measuring of cantilever deflection. We use conventional technique of measuring the change in angle of light reflected off the cantilever with an additional lens placed between cantilever and four-segment photodiode, The lens forms an image of the cantilever in the photodiode plane. Small sizes of the cantilever image improve the resolution of AFM and reduce the requirements to the optical scheme of the microscope. The lens non-linear transmission of the cantilever deflection is compensated by means of computer program. With the A FM were imaged the DNA of plague microbes (Y. pestis EV) and phages of plague and V. Cholera.

关键词： atomic force microscopy cantilever deflection methods phage DNA biology

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acquisition of multiple image stacks with a confocal laser scanning microscope

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Zuschratter, W Steffen, T Braun, K Herzog, A Michaelis, B Scheich, H Fed Inst Neurobiol D-39118 Magdeburg Germany

ISBN: (纸本)0819427004

image acquisition at high magnification is inevitably correlated with a limited view over the entire tissue section. To overcome this limitation we designed software for multiple image-stack acquisition (3D-MISA) in confocal laser scanning microscopy (CLSM). The system consists of a 4 channel Leica CLSM equipped with a high resolution z-scanning stage (Leica Lasertechnik) mounted on a xy-motorized stage (Marzhauser). The 3D-MISA software is implemented into the microscope scanning software (Scanware, Leica Lasertechnik) and uses the microscope settings (magnification, zoom) for the movements of the xy-stage. It allows storage and recall of 70 xyz-positions and the automatic 3D-scanning of image arrays between selected xyz-coordinates. The number of images within one array is limited only by the amount of disk space or memory available. Although for most applications the accuracy of the xy-scanning stage is sufficient for a precise alignment of tiled views, the software provides the possibility of an adjustable overlap between two image stacks by shifting the moving steps of the xy-scanning stage. After scanning a tiled image gallery of the extended focus-images of each channel will be displayed on a graphic monitor. In addition, a tiled image gallery of individual focal planes can be created. In summary, the 3D-MISA allows 3D-image acquisition of coherent regions in combination with high resolution of single images.

关键词： multi-image-stacks confocal laserscanning microscopy 3D image acquisition

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On-line multi-spectral image analysis using unsupervised neural network

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Lu, T Tan, SZ Lu, RQ Lerner, JM In Harmony Technol Corp Petaluma CA 94952 USA

ISBN: (纸本)0819427004

The overwhelming size of a hyperspectral image creates serious problem for users to understand and utilize the data. A novel unsupervised neural network (UNN) model is presented. The UNN is designed to analyze the spectral contents of the multi-spectral images. The UNN automatically grows its layers and neurons by scanning the training images and by learning spectral features. The learning strategy is optimized to ensure fast convergence. At the end of learning, the UNN provides a table of the spectral content of the images. The image contents are categorized based on spectral similarities. The resulting spectral classes are then mapped onto the image, thus the UNN effectively compresses a hyperspectral image cube into a single image. The UNN is also able to automatically recognize objects by their spectral features. The ability of the UNN in identifying subtle spectral differences is shown.

关键词： hyperspectral multispectral image analysis unsupervised neural networks automated object recognition

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Data correction methods for 3D light microscopy

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V

作者： Wang, CL O'Connor, NJ Wang, M Turner, JN Holmes, TJ AutoQuant Imaging Inc Watervliet NY 12189 USA

ISBN: (纸本)0819427004

A cooled or video-rate CCD camera is often used to collect optical slices from many modalities of microscopy including widefield-fluorescence (WF) and transmitted-light brightfield (TLB). Raw optical slices collected from both of these types of cameras are contaminated by imperfect performance of the CCD camera. Optical slice image data must be corrected for the bias level and nonuniformity in the photometric response of CCD elements (flatfield). Fluctuation of the exposure time in a cooled CCD camera needs to be calibrated as well. Bad pixels due to the severely low sensitivity of some of the CCD elements often occur and otherwise impair the conventional schemes of correction and calibration. An adaptive median filter has been introduced by us previously to treat these bad pixels. In this study a Wiener-type regularization scheme is proposed as robust, fast, and practical alternative treatment of these bad pixels. One advantage of using this scheme is that doing so provides a nicely modularized design of the computer algorithm. As such, the correction scheme is software readily integrated as a software component in a three-dimensional (3D) microscopy system and may operate independently without affecting the design of other software components in the system.

关键词： data correction 3D light microscopy adaptive median filter Wiener-type regularization

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