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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX"

共 591 条记录，以下是61-70 订阅

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three dimensional refractive index imaging with differential interference contrast microscopy

Three dimensional refractive index imaging with differential...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing Xix

作者： Aung, Htet Buckley, Jared Kostyk, Piotr Rodriguez, Braulio Phelan, Shelley Xu, M. Fairfield Univ Dept Phys 1073 N Benson Rd Fairfield CT 06824 USA

ISBN: (纸本)9780819488701

We report here a new approach based on an extension of the transport of the intensity equation for three dimensional refractive index imaging of a weak phase object from a series of images recorded by a differential interference contrast microscope at different focus (z-stack). Our method is first validated by imaging polystyrene spheres. We then apply this method to monitor in vivo apoptosis of human breast MCF7 epithelial cells. The potential applications are discussed at the end.

关键词： DIC microscopy intensity propagation equation quantitative phase imaging 3D refractive index map

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Numerical simulation of image formation in near-field optical microscopy

Numerical simulation of image formation in near-field optica...

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three-dimensional and multidimensional microscopy: image acquisition and processing ix

作者： Baibyrin, Vil B. Volkov, Uryi P. Avetisyan, Uryi A. Krasnikova, Irina V. Bespalova, Natalija V. Saratov State Technical University Politechnitcheskaja 77 Saratov 410007 Russia

Numerical simulation of image formation in near field optical microscopy is needed to understand a relationship between near field images and actual structure of sample since the image can be differs strongly from real structure. In order to estimate the near field image formation, two different approaches are used, namely numerical solution of integral equations with boundary element method or moment method and analytical solution of light scattering problem obtained by assumption of some hypotheses. The first approach results in reasonably complicated algorithm of calculation and often causes a bed conditioned system of linear equations to be formed. The approach based on an analytical solution of scattering problem is more preferable since allow to reduce the algorithm and increase the calculations speed. A new program of simulation of near field image formation for arbitrary sample shape has been developed. The program is based on the results of analytical calculation of near field light diffraction on sinusoidal diffraction grating of arbitrary corrugation amplitude. The sample shape is presented as a number of sinusoidal gratings of various amplitudes by Fourier transformation, for each of the gratings the light scattering amplitude is calculated and the amplitudes are summarized.

关键词： Near field scanning optical microscopy

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Adaptive optics for three-dimensional optical data storage and micromachining

Adaptive optics for three-dimensional optical data storage a...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Booth, Martin J. Schwertner, Michael Wilson, Tony Univ Oxford Dept Engn Sci Parks Rd Oxford OX1 3PJ England

ISBN: (纸本)0819461326

Many high resolution optical methods are affected by the presence of optical aberrations. These include microscopy, three-dimensional optical data storage and optical micromachining. We investigate the use of adaptive aberration correction applied to these techniques. In particular, it is shown how a deformable membrane mirror can be used to correct the aberrations when focusing deep into a multilayer optical data storage medium for both recording and read-out of data. Aberration correction for optical micromaching deep inside a substrate is also demonstrated.

关键词： Aberration correction Optical storage Monochromatic aberrations Adaptive optics Micromachining Microscopes 3D optical data storage Confocal microscopy Data storage Objectives

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High-speed focal modulation microscopy using acousto-optical modulators for visualization of thick biological specimens

High-speed focal modulation microscopy using acousto-optical...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Chong, Shau Poh Gao, Guangjun Sheppard, Colin J. R. Chen, Nanguang Natl Univ Singapore Div Bioengn Singapore 117576 Singapore

ISBN: (纸本)9780819484413

In recent development of fluorescence microscopy, the out-of-focus fluorescence background that arises when imaging deep inside biological tissues is critical in determining the image quality and penetration depth. Focal modulation microscopy (FMM) is an advanced fluorescence technique that can provide high subcellular resolution when imaging thick specimens mainly by preserving the signal-to-background ratio.

关键词： Fluorescence microscopy multiple scattering penetration depth

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Scanning microscopy with extended depth of focus

Scanning microscopy with extended depth of focus

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Juskaitis, R Botcherby, EJ Wilson, T Univ Oxford Dept Engn Sci Oxford England

We demonstrate a simple and light-efficient way of generating non-diffracting Bessel beams for use in confocal microscopy. A number of imaging modalities using such beams is discussed. Preliminary experimental results... 详细信息

ISBN: (纸本)0819456756

关键词： confocal extended depth of focus Bessel beam fluorescence microscopy polarisation multi-photon

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Optical design of a snapshot high-sampling image Mapping Spectrometer (IMS) for hyperspectral microscopy

Optical design of a snapshot high-sampling Image Mapping Spe...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Gao, Liang S. Kester, Robert T. Tkaczyk, Tomasz S. Rice Univ Dept Bioengn Houston TX 77005 USA

ISBN: (纸本)9780819479662

A snapshot high-sampling image Mapping Spectrometer (IMS) is developed for hyperspectral microscopy, measuring datacube of dimensions 285x285x60 (x,y,lambda). microscopy IMS is designed to work at the Nyquist sampling limit to realize high resolution imaging. The spatial resolution is similar to 0.45 mu m with FOV similar to 130 mu m. The spectral working range is 500-700nm with similar to 8.3nm average spectral resolution. Preliminary tests have been implemented on its imaging performances.

关键词： Hyperspectral microscopy image Mapping Spectrometer

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three-dimensional state space realization algorithm: noise suppression of fluorescence microscopy images and point spread functions

Three-dimensional state space realization algorithm: noise s...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Lai, XM Ward, ES Lin, ZP Ober, RJ Univ Texas SW Med Ctr Ctr Immunol Dallas TX 75235 USA

ISBN: (纸本)0819456756

A recently developed algorithm is applied to calculate a state space realization of a 3D microscopy image set. It is based on interpreting the image set as the impulse response of a 3D se arable system. As an application it is shown how this algorithm. combined with approximation steps, can be used to suppress noise in 3D experimental point spread functions. The approach was motivated by a well known problem that a noisy point spread function degrades the results of deconvolution algorithms for the restoration of 3D fluorescence microscopy image sets. The proposed approach can also be applied to 3D fluorescence microscopy image sets of cells.

关键词： point spread function estimation state space algorithm noise suppression

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Lateral resolution enhancement in confocal self-interference microscopy

Lateral resolution enhancement in confocal self-interference...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Kang, D Gweon, D Korea Adv Inst Sci & Technol Med Engn Dept NOM Lab Taejon 305701 South Korea

ISBN: (纸本)0819456756

Lateral resolution enhancement in confocal self-interference microscopy (CSIM) is evaluated. CSIM, which uses the birefringence of the calcite plate to generate self-interference pattern, sharpens the central lobe of the effective spot. Numerical simulation results of two-dimensional imaging performances are presented. Two-point resolution of 149nm is achieved, which is enhanced by nearly 100% compared to that of confocal microscopy.

关键词： confocal microscopy superresolution birefringence self-interference

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Fluorescence microscopy with isotropic resolution using three objectives 13

Fluorescence microscopy with isotropic resolution using thre...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Huelsnitz, Thomas Kner, Peter Univ Georgia Coll Engn Athens GA 30602 USA

ISBN: (纸本)9781628419474

Widefield and confocal fluorescence microscopy using a single objective suffer from poor resolution and a strong anisotropy between the lateral and axial resolution. Coherently combining the excitation and emission from two coaxial objectives improves the axial resolution up to sevenfold, but leaves the lateral resolution unchanged. Here we investigate the coherent combination of three objectives to create a point spread function (PSF) that is isotropic with higher resolution in the plane of the objectives. We develop a theoretical framework for simulating the performance of interferometric imaging with three objectives. Using three identical objectives with a large working distance and 0.9 numerical aperture (NA), the full-width half maximum of the confocal PSF is 135 nm compared to the lateral FWHM of 274 nm for imaging with a single objective at a wavelength of 515 nm.

关键词： microscopy fluorescence microscopy interferometric imaging PSF Engineering

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three-dimensional measurement of microorganism by retardation modulated differential interference contrast microscope

Three-dimensional measurement of microorganism by retardatio...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Aoki, G. Itoh, M. Yasuno, Y. Yatagai, T. Univ Tsukuba Inst Appl Phys 1-1-1 Tennodai Tsukuba Ibaraki 3958573 Japan

ISBN: (纸本)0819461326

We propose a new technique for obtaining three-dimensional phase distribution on differential interference contrast microscope to modulate relative phase retardation between two shear beams. Using partial coherent theory we extract the phase information from two different retardation images. For the object in a weak phase region, simple formula is derived. The images of nematomorph were obtained in vivo.

关键词： differential interference contrast retardation modulated phase measurement microscope Nomarski

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