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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI"

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three-dimensional imagery using multiple nanometric probes

Three-dimensional imagery using multiple nanometric probes

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Moneron, G Dubois, A Boccara, AC Ecole Super Phys & Chim Ind Ville Paris Lab Opt Phys CNRS UPR A0005 F-75231 Paris 5 France

ISBN: (纸本)0819452327

We present a high-resolution imaging technique using nanometric beads as multiple scattering local probes. The positions of the beads are determined in three dimensions by white-light interference microscopy. The technique has been applied to study the deformation of gels under mechanical constraint. The location of Brownian moving beads has also been demonstrated with nanometer spatial precision and 10 mus acquisition time. High-resolution 3-D imaging of hollow structures explored by the beads in relatively transparent materials should be possible.

关键词： interference microscopy three-dimensional microscopy scanning probe microscopy

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Fluorescence lifetime imaging microscopy based on a synchroscan streak camera

Fluorescence lifetime imaging microscopy based on a synchros...

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Qu, J Li, J Niu, HB Shenzhen Univ Inst Optoelect Shenzhen 518060 Guangdong Prov Peoples R China

ISBN: (纸本)0819452327

A new time-domain two-dimensional fluorescence lifetime detection method and a fluorescence lifetime imaging microscope that is based on a synchroscan streak camera are presented. This system can generate in parallel a complete two-dimensional fluorescence lifetime image without scanning the light. We demonstrate the acquisition of a fluorescence lifetime image of a semiconductor ET material sample at a temporal resolution of 2ps and spatial resolution of 4mum within 40ms.

关键词： fluorescence lifetime fluorescence lifetime imaging fluorescence lifetime imaging microscopy synchroscan scan streak camera temporal resolution spatial resolution

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Nano-scale optical imaging technique

Nano-scale optical imaging technique

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Wu, S Tang, YC PEER Ctr Covina CA 91722 USA

ISBN: (纸本)0819452327

The optical resolution limits the observation of fine features with size below diffraction limit, e.g. 500nm. We propose and demonstrate a technique that uses collimated laser scattering to characterize such features. We use a new illumination technique that let us image bio-molecules down to 50nm with optical microscopy. We observed vesicles with size down to 50nm and visualize the flow of such tiny molecules in the live cells. We could also characterize the detailed size and molecular weight of such particles.

关键词： single particle laser scattering microscopy

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Fast 3D blind deconvolution of even point spread functions

Fast 3D blind deconvolution of even point spread functions

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Yagle, AE Shah, S Univ Michigan Dept EECS Ann Arbor MI 48109 USA

ISBN: (纸本)0819452327

3-D mages obtained from optical sectioning microscopy are usually degraded by a point-spread function that is known to be an even function but is otherwise only approximately known, or even entirely unknown. We present a new algorithm for 3-D blind deconvolution of even point-spread functions that is both fast and (in the absence of noise) exact. Fourier transforms decouple the problem into 2-D, then 1-D blind deconvolution problems, greatly increasing computational speed. Numerical simulations demonstrate that the blind algorithm seems to perform both faster and more accurately than the non-blind iterative Lucy-Richardson algorithm.

关键词： Deconvolution Point spread functions 3D image processing Signal to noise ratio Convolution microscopy Fourier transforms Confocal microscopy image filtering Matrices

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Automatic quantification of neurite outgrowth by means of image analysis

Automatic quantification of neurite outgrowth by means of im...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Van de Wouwer, G Nuydens, R Meert, T Weyn, B Univ Antwerp Visielab Dept Phys B-2020 Antwerp Belgium

ISBN: (纸本)0819452327

A system for quantification of neurite outgrowth in in-vitro experiments is described. The system is developed for routine use in a high-throughput setting and is therefore needs fast, cheap, and robust. It relies on automated digital microscopical imaging of microtiter plates. image analysis is applied to extract features for characterisation of neurite outgrowth. The system is tested in a dose-response experiment on PC12 cells + Taxol. The performance of the system and its ability to measure changes on neuronal morphology is studied.

关键词： automation microscopy image analysis neurite outgrowth

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Point spread function models for digital holographic microscopy

Point spread function models for digital holographic microsc...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Marian, A Depeursinge, C Swiss Fed Inst Technol Inst Biomed Imaging Opt & Engn CH-1015 Lausanne Switzerland

ISBN: (纸本)0819452327

Few models, based on the diffraction theory, are proposed in order to evaluate the point spread function of different microscope objectives used in a digital holographic microscope. Because in holography the phase information is essential, a 3D amplitude point spread function (APSF), modulus and phase, is necessary, in order to properly deconvolute the 3D images obtained. Scalar Debye theory, paraxial approximation and vectorial Debye theory are used to solve the diffraction problem and the theoretical predicted 3D APSFs obtained. with these models are compared.

关键词： point spread function diffraction digital holographic microscopy

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Real-time digital design for an optical coherence tomography acquisition and processing system

Real-time digital design for an optical coherence tomography...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Ralston, TS Mayen, JA Marks, D Boppart, SA Univ Illinois Beckman Inst Adv Sci & Technol Urbana IL 61801 USA

ISBN: (纸本)0819452327

We present a real-time, multi-dimensional, digital, optical coherence tomography (OCT) acquisition and imaging system. The system consists of conventional OCT optics, a rapid scanning optical delay (RSOD) line to support fast data acquisition rates, and a high-speed A/D converter for sampling the interference waveforms. A 1M-gate Virtex-II field programmable gate array (FPGA) is designed to perform digital down conversion. This is analogous to demodulating and low-pass filtering the continuous time signal. The system creates in-phase and quadrature-phase components using a tunable quadrature mixer. Multistage polyphase finite impulse response (FIR) filtering and down sampling is used to remove unneeded high frequencies. A floating-point digital signal processor (DSP) computes the magnitude and phase shifts. The data is read by a host machine and displayed on screen at real-time rates commensurate with the data acquisition rate. This system offers flexible acquisition and processing parameters for a wide range of multi-dimensional optical microscopy techniques.

关键词： digital signal processing optical coherence tomography Doppler spectroscopic real-time

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Microscopic tomography by digital interference holography

Microscopic tomography by digital interference holography

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Kim, MK Univ S Florida Dept Phys Tampa FL 33620 USA

ISBN: (纸本)0819452327

We are developing the digital interference holography (DIH) as a novel method of microscopic tomographic imaging by numerical superposition of a number of holographic fields taken with varying wavelengths. The digital interference holography does not involve pixel-by-pixel mechanical scanning of three-dimensional volume and yet achieves high lateral and longitudinal resolutions. The holographic interference pattern of an ob ect is generated optically and recorded digitally using a CCD camera. The hologrpahic image field is numerically calculated using basic diffraction formulas and the process is repeated for a range of varying wavelengths at regular intervals. Numerical superposition, or digital interference, of the holographic image fields yields the desired three-dimensional representation of the object. Experiments have demonstrated a few-micron lateral and axial resolutions. Furthermore, since the DIH is a coherent imaging system, one can form true tomographic images of sub-surface structures, in the presence of diffuse scattering from overlying layers. By being able to generate true tomographic images of subsurface structures, without the need for three-dimensional mechanical scannig, the DIH method can provide a very efficient and versatile imaging modality for a wide range of applications in materials science and biomedical imaging.

关键词： digital holography tomography microscopy multi-wavelength imaging

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A phase-shifting DIC technique for measuring 3D phase objects: Experimental verification

A phase-shifting DIC technique for measuring 3D phase object...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： King, SV Cogswell, CJ Univ Colorado Dept Elect & Comp Engn Boulder CO 80309 USA

ISBN: (纸本)0819452327

Experimental verification of our previously proposed linear phase imaging technique for differential interference contrast microscopy (DIC) microscopy is presented. This technique first applies phase-shifting methods to DIC to acquire linear phase gradient images in two orthogonal directions. A special Fourier integration algorithm is then applied to the combined phase gradient images to create a single linear phase image in which intensity is proportional to phase. This overcomes the limitations of traditional DIC, which cannot accurately measure the phase (i.e. refractive index or thickness) of embedded 3D phase objects. The linear phase imaging technique is implemented using a standard DIC microscope altered to allow controlled phase shifting, a low noise CCD camera, and post-processing in Matlab. The results presented confirm the linear proportionality of intensity to phase in these images.

关键词： DIC phase phase gradient phase imaging spiral phase interference microscopy

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Agile optical confocal microscopy instrument architectures for high flexibility imaging

Agile optical confocal microscopy instrument architectures f...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Riza, NA Bokhari, A Nuonics Inc Orlando FL 32817 USA

ISBN: (纸本)0819452327

Ideally, a no-moving parts fast and agile scanning confocal microscope system is required that can produce true realtime 3-D scans with precision and repeatability. In this paper, such agile optical confocal microsopy designs are proposed that enable high speed precise non-invasive 3-D imaging. These compact confocal microscopes can provide real-time pin-point focussed imaging to enable confocal slices in-vivo, thus greatly reducing motion artifacts. These microscopes can be modified into interferometric microscopes for phase contrast imaging. The proposed microscopes can also greatly improve confocal fluorescence imaging as needed for cancer detection. An ultracompact confocal probe tip connected to a single ultra-thin fiber is another design option allowing flexibility for usage in tight cavities.

关键词： confocal microscopy fiber-optics instrument 3-D imaging

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