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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI"

共 593 条记录，以下是41-50 订阅

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A novel multimodal laser scanning microscope control system 22

A novel multimodal laser scanning microscope control system

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XxiI

作者： Lai, Zhenhua Gu, Zetong Karasek, Stethen McLean, James Zhang, xi DiMarzio, Charles Yin, Jihao xiong, Daxi Chinese Acad Sci Suzhou Inst Biomed Engn & Technol Jiangsu Key Lab Med Opt Suzhou 215163 Peoples R China Northeastern Univ Dept Elect & Comp Engn Boston MA 02115 USA Northeastern Univ Dept Mech & Ind Engn Boston MA 02115 USA Beihang Univ Sch Astronaut Beijing 100191 Peoples R China

ISBN: (纸本)9781628414202

Traditional laser scanning microscopes require complex control systems to synchronize and control image acquisition. The control system is especially cumbersome in the multimodal laser scanning microscope. We have developed a novel multimodal laser scanning microscope control system based on a National Instruments multifunction data acquisition device (DAQ), which serves as both a data acquisition device and a programmable signal generator. The novel control system is low-cost and easy-to-build, with all components off-the-shelf. We have applied the control system in a multimodal laser scanning microscope. The control system has not only significantly decreased the complexity of the microscope, but also increased the system flexibility. We have demonstrated that the system can be easily customized for various applications.

关键词： Laser scanning multimodal microscopy microscope confocal multi-photon fluorescence 2-photon fluorescence SMPAF

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Bright field segmentation tomography (BFST) for use as surface identification in stereomicroscopy

Bright field segmentation tomography (BFST) for use as surfa...

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Thiesse, J Namati, E de Ryk, J Hoffman, E McLennan, G Univ Iowa Iowa City IA 52242 USA

ISBN: (纸本)0819452327

Stereomicroscopy is an important method for use in image acquisition because it provides a 3D image of an object when other microscopic techniques can only provide the image in 2D. One challenge with this type of imaging is determining the top surface of a sample that has otherwise indistinguishable surface and planar characteristics. We have developed a system that creates a form of oblique illumination and in conjunction with image processing, the top surface can be viewed. The use of BFST consists of the Leica MZ16A stereomicroscope with a unique attached lighting source, and is part of the Large image Microscope Array (LIMA) [1]. The lighting source consists of eight light emitting diodes (LED's) that are separated by 45-degree angles. Each LED in this system illuminates with a 20-degree viewing angle once per cycle with a shadow over the rest of the sample. Subsequently, eight segmented images are taken per cycle. After the images are captured they are stacked through image addition to achieve the full field of view, and the surface is then easily identified. image processing techniques, such as skeletonization, can be used for further enhancement and measurement. With the use of BFST, advances can be made in detecting surface features from metals to tissue samples, such as in the analytical assessment of pulmonary emphysema using mean linear intercept.

关键词： stereomicroscopy illumination surface identification image processing

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The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes

The three-dimensional imaging of microspheres in confocal an...

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Yang, LS Taylor, CM Rakovich, Y McCabe, EM Univ Dublin Trinity Coll Dept Phys Dublin 2 Ireland

ISBN: (纸本)0819447641

images of the microspheres are studied in three-dimensions by using the confocal and conventional scanning polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.

关键词： three-dimensional microscopy polarization microsphere

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Mueller polarimetric microscopy

Mueller polarimetric microscopy

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Laude-Boulesteix, B De Martino, A Le Naour, G Genestie, C Schwartz, L Garcia-Caurel, E Drévillon, B Ecole Polytech Phys Interfaces & Couches Minces Lab CNRS UMR 7647 F-91128 Palaiseau France

ISBN: (纸本)0819452327

We present a multispectral polarimetric imagine, system well suited for complete Mueller matrix microscopy. The source is a spectrally filtered halogen light bulb, and the image is formed on a fast CCD camera The light polarization is modulated before the sample and analyzed after the sample by using nematic liquid crystal modulators.. The whole Mueller matrix image of the sample is typically measured over 5 seconds for a good signal-to-noise ratio. The instrument design, together with an original and easy-to-operate calibration procedure provides a high polarimetric accuracy over wide ranges of wavelengths and magnifications. Mueller polarimetry provides separate images of scalar and vector retardation and dichroism of the sample, together with its depolarizing power, while all these effects do contribute simultaneously to the contrasts observed in standard polarized microsopy. Polarimetric images of several samples, namely an unstained rabbit cornea, a picrosirius red stained hepatic biopsy, and a rat artery specifically stained for collagen III are shown and discussed.

关键词： imaging systems polarimetry medical optics and biotechnology histology

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Object segmentation, quantification, counting, and tracking

Object segmentation, quantification, counting, and tracking

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Abu-Tarif, A Khiznichenko, V Northan, B Holmes, T Yoon, P Girroir, J Brathwaite, P AutoQuant Imaging Inc Watervliet NY 12189 USA

ISBN: (纸本)0819452327

A complete system for object segmentation, counting, quantification, and tracking from microscopic images was implemented. We found that image deconvolution and reconstruction operations are essential to the success of any general-purpose segmentation algorithm and hence are of paramount importance for a counting and tracking software system. Wavelet-based image enhancement, background equalization, and noise suppression routines are the components in our novel general-purpose segmentation algorithm. Simple object recognition based on averages and preset tolerances suffices for most applications. As expected, boundary smoothing is important if watershed-based blob separation is to be used. One of the challenges of a general-purpose counting and tracking system is the need for a large number of object quantification components (features). In tracking we found that incorporating weighted features into an error function improves the accuracy over just the path coherence criterion and that evaluating correspondences over multiple time frames improves the accuracy over using only two consecutive time frames.

关键词： deconvolution image reconstruction segmentation object counting tracking

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4D image processing in microscopy by combined methods

4D image processing in microscopy by combined methods

引用

Conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Kriete, A Image Proc Lab Inst Anat & Cell Biol D-35385 Giessen Germany

ISBN: (纸本)081943535X

In time-lapse microscopy image quality is limited by instrumental and specimen characteristics. Moreover, an enormous amount of data may be accumulated. This challenges not only the development of new tools for image processing, but typically forces methods to be executed in combination. To remedy essential problems, an image processing pipeline is employed which features image restoration, image compression, visualization and analysis. To control the flow of information within this pipeline and to optimize the parameter settings like compression rates or number of iterations, the information content is evaluated on the basis of the ratio of signal and noise.

关键词： time-lapse microscopy 4D image processing compression image restoration visualization and analysis

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image estimation for structured-illumination microscopy

Image estimation for structured-illumination microscopy

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xiI

作者： Conchello, JA Oklahoma Med Res Fdn Program Mol Cell & Dev Biol Oklahoma City OK 73104 USA

ISBN: (纸本)0819456756

When recording three-dimensional (3D) images by the method of optical sectioning microscopy, each optical section contains the in-focus information plus out-of-focus contributions that obscure the in-focus detail and reduce contrast. There are several methods to remove or prevent the out-of-focus contributions from the stack of optical sections. One such method is image estimation -the use of a computer program based on a mathematical description of the microscope to remove the out-of-focus contributions. Another method is the use of structured illumination and a simple arithmetic operation to obtain a image that in which the out-of-focus contributions are greatly reduced. We derived a method for image estimation that uses the images collected from the structured-illumination microscope. The method improves the resolution of small detail over that possible with the structured illumination using the simple arithmetic formula.

关键词： image analysis Expectation maximization algorithms Microscopes microscopy 3D image processing Confocal microscopy image acquisition image processing Point spread functions Luminescence

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Phase imaging with partially coherent light

Phase imaging with partially coherent light

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Waller, Laura Univ Calif Berkeley Dept Elect Engn & Comp Sci Berkeley CA 94702 USA

We review several quantitative phase imaging methods that retrieve phase from a through-focus series of intensity images (specifically, iterative methods and Transport of Intensity) and compare their performance when ... 详细信息

ISBN: (纸本)9780819493583

关键词： Phase imaging partial coherence microscopy transport of intensity

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Digital holographic confocal microscope

Digital holographic confocal microscope

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Goy, Alexandre S. Psaltis, Demetri Ecole Polytech Fed Lausanne CH-1015 Lausanne Switzerland

ISBN: (纸本)9780819493583

We demonstrate experimentally a scanning confocal microscopy technique based on digital holographic recording of the scanned spot. The data collected in this way contains all the information to produce three-dimensional images. Several methods to treat the data are presented, such as the dynamic placement of the pinhole. Examples of reflection and transmission images of epithelial cells and mouse brain tissue are shown. The computations can be performed in real time, the speed being limited only by the frame rate of the camera. This method enables a convenient implementation of confocal microscopy, especially in transmission as no de-scan device is required.

关键词： digital holography confocal microscopy three-dimensional imaging

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Efficient calibration algorithm and calibration pattern for correcting distortions for three-dimensional image acquisition systems for microscopic applications

Efficient calibration algorithm and calibration pattern for ...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Walecki, Wojtek J. Szondy, Fanny Sunrise Opt LLC Sunrise FL 33351 USA

ISBN: (纸本)9780819470362

We present novel method for fully automated detection of calibration features. It reduces computation effort when compared to traditional corner, edge, and conic detection algorithms. Our method employs generalized method based on properties of prospective projection invariants [1], but is also applicable for the optical systems suffering high order, non-spherical distortions. We also show an example when in presence of significant barrel distortion procedure developed for ideal pinhole camera system outlined in [1] does not converge to Unique solution. We suggest new modified algorithm which produces unique solution.

关键词： machine vision camera calibration image processing

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