检索结果-内蒙古大学图书馆

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Chamgoulov, RO Lane, PM MacAulay, CE British Columbia Canc Res Ctr Canc Imaging Dept Vancouver BC V5Z 1L3 Canada

ISBN: (纸本)0819452327

A computed-tomography microscope using a digital spatial light modulator has been developed and has demonstrated cytological and histological three-dimensional (3-D) image acquisition. The system consists of two high NA objective lenses, a Digital Micromirror Device (DMD) placed conjugate to the back pupil plane of the illumination objective, a sample stage, a light source, and a CCD detector. Each DMD micromirror can control the illumination of a specific angle. A single 3-D reconstruction is obtained from parallel ray projections acquired by changing the polar angle of illumination -phi (max) less than or equal to phi less than or equal to phi (max) while holding the azimuthal angle theta constant. The polar angle is limited by the NA of the objective. To compensate for an incompleteness of information due to the limited polar angles, several reconstructions acquired at multiple azimuthal angles are combined to create a final reconstruction. A reconstruction algorithm was developed using simulation software based on the 3-D Radon transformation and 3-D synthetic objects. Microscopic 3-D volume reconstructions of quantitatively absorption-stained cells have been demonstrated. 3-D reconstructed images enables the analysis of cell morphology and tissue architecture, as well as virtual two-dimensional slices with the distance between slices of 0.3 mum.

关键词： computed-tomography optical microscopy 3-D imaging Radon transformation digital spatial light modulator

来源：评论

学校读者我要写书评

暂无评论

Development of micro-optical projection tomography for 3D analysis of single cells

Development of micro-optical projection tomography for 3D an...

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Fauver, M Seibel, EJ Rahn, JR Patten, FW Nelson, AC Univ Washington Coll Engn Human Interface Technol Lab Seattle WA 98195 USA

ISBN: (纸本)0819452327

The Micro-Optical Projection Tomographic Microscope (muOPTM) is an instrument that is being developed for three-dimensional (3D) imaging of cells and subcellular components. The target application for the muOPTM is the early detection of lung cancer by revealing the complex 3D information about chromatin redistribution in the nucleus. The muOPTM employs a scanning objective lens (100x, N.A.=1.25) to create an extended depth-of-field image, similar to a shadowgram or projection, that we call a pseudo-projection. A large number of pseudoprojections (90+) are acquired, from which a tomographically reconstructed 3D image is computed using a filtered backprojection algorithm. The prototype muOPTM uses a single objective lens, so the object (cell) must be rotated for each new pseudo-projection. A custom microtube stage minimizes the lateral and axial motion of the sample tube during scanning and rotation so that registration between successive pseudo-projections is maintained. image processing methods are used to correct any remaining registration errors. The media inside and outside the tube are refractive index-matched to each other and to the tube (Deltan(avg) < 0.02). The index-matched materials are pressed between two flat parallel windows, providing a nearly distortion-free image. Custom phantoms using microspheres have been constructed and images of these 3D test targets acquired. The minimum resolvable feature size is better than 3 microns. The first 3D image of a cell using muOPTM is also shown.

关键词： optical tomography optical projection tomography three-dimensional imaging computed tomography microscopy depth of field cancer screening

来源：评论

学校读者我要写书评

暂无评论

High-speed image acquisition synchronized with the motion of Galvanometer scanner for confocal microscopy - art. no. 686116

High-speed image acquisition synchronized with the motion of...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XV

作者： Bae, Yoon Sung Moon, Sucbei Kim, Dug Y. Gwangju Inst Sci & Technol Dept Informat & Commun Kwangju 500712 South Korea

ISBN: (纸本)9780819470362

We present a simple 2D image acquisition technique electronically implemented for laser scanning confocal microscope using galvanometer scanners. In order to synchronize image acquisition process with the movement of the galvanometer scanner, position signal of the mirror of the galvanometer scanner is used and manipulated for generating of sync-signals. This is achieved using an analog-digital converter to read a position signal from the scanner which tells about its position and to generate a trigger signal (or pixel clock) which tells the moment of digitizing the received analog signal from the photo-detector. This facilitates processing the image in synchronization with the actual motion of the scanning laser beam scanner. image construction is performed by a video acquisition board (frame grabber). The newly developed scanning and image acquisition systems are implemented in a confocal microscope with fiber-optic components for compact configuration and flexible light path.

关键词： confocal microscopy image acquisition galvanometer scanner

来源：评论

学校读者我要写书评

暂无评论

three-dimensional super-resolution imaging of single molecules by spectrally selective confocal microscopy

引用

Proceedings of SPIE - The International Society for Optical Engineering 1999年 3605卷 82-89页

作者： Brakenhoff, G.J. van Oijen, A.M. Kohler, J. Muller, M. Schmidt, J. Univ of Amsterdam Amsterdam Netherlands

ISBN: (纸本)0819430757;9780819430755

It is shown that based on spectrally selective excitation of individual molecules in the focus of a high NA lens together with position sensitive imaging sub-resolution imaging of three-dimensional structures can be realised. The feasibility of the idea is demonstrated with NA=0.55 optics on a model system of pentacene molecules in p-terphenal host matrix.

关键词： Optical microscopy

来源：评论

学校读者我要写书评

暂无评论

Standards in digital microscopy

Standards in digital microscopy

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Kriete, A Shah, S Inst Anat & Cell Biol IPL D-35385 Giessen Germany

This paper discusses some aspects of standardization in digital microscopy. Included are image acquisition, use of image and graphics formats and data protocols in multidimensions, particular image analysis procedures... 详细信息

ISBN: (纸本)0819439398

关键词： Standards microscopy image acquisition image formats image processing

来源：评论

学校读者我要写书评

暂无评论

Single-cell volume estimation by applying three-dimensional reconstruction methods

引用

Proceedings of SPIE - The International Society for Optical Engineering 1999年 3605卷 146-157页

作者： Khatibi, S. Allansson, L. Gustavsson, T. Blomstrand, F. Hansson, E. Olsson, T. Chalmers Univ of Technology

ISBN: (纸本)0819430757;9780819430755

The use of three-dimensional reconstruction methods to estimate the cell volume of astroglial cells in primary culture was studied. Two-dimensional microscopic images were collected by using an automated image-acquisition system. The images were reconstructed by the Linear Maximum a Posteriori method and the non-linear Maximum Likelihood Expectation Maximization (ML-EM) method. A fast variant of the ML-EM method was also developed. Results of this study show that the ML-EM reconstructed images are adequate for the determination of volume changes in cells or parts thereof.

关键词： image reconstruction

来源：评论

学校读者我要写书评

暂无评论

image enhancement for digital visualization of three dimensional integral imaging

Image enhancement for digital visualization of three dimensi...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xiII

作者： Ponce-Diaz, Rodrigo Alvarez-Elizondo, Martha Saenz, Julia Javidi, Bahram Inst Tecnol & Estudios Super Monterrey Av Eugenio Garza Sada 2501CP 64849 Monterrey NL Mexico Univ Connecticut Storrs CT 06269 USA

ISBN: (纸本)0819461326

In this work, we are proposing the use of digital image enhancement in three dimensional (3D) Integral Imaging (InI) applying in small objects visualization. We used a well known image filter algorithm for enhance the edges and detail information of the 3D reconstruction InI image via unsharp masking. Small objects as bugs were recorded in an elemental image array, image processed and digital reconstructed as 3D objects. We implemented the algorithm over the elemental image array as usual and using an innovative technique that involves a simple digital reconstruction algorithm by quadruple pixel extraction. Digital results show an improvement in details visualization, which have potential application in 3D microscopy.

关键词： integral imaging image enhancement three dimensional processing

来源：评论

学校读者我要写书评

暂无评论

three-dimensional data acquisition with aberrations correction for video-rate microscopy

Three-dimensional data acquisition with aberrations correcti...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Samim, Masood Cisek, Richard Sandkuijl, Daaf Tretyakov, Ian Siu, Sam Musikhin, Sergey Barzda, Virginijus Univ Toronto Dept Chem & Phys Sci Mississauga ON L5L 1C6 Canada

ISBN: (纸本)9780819484413

We demonstrate a multimodal, multifocal, differential nonlinear optical microscope, which is equipped with a pair of deformable mirrors and a Shack-Hartmann sensor for dynamic wavefront manipulation. The optical wavefronts of a home built Yb:KGW femtosecond (1028 nm) laser-beams are engineered to perform multidepth focusing in differential mode with simultaneous corrections for optical aberrations. The 39-actuator deformable mirrors provide fast reshaping of the wavefront and optical aberrations correction of the diffraction-limited focal volume allowing for fast axial scanning. Combination of similar to 200 frames per second lateral scanning with fast refocusing enables a three-dimensional video rate scanning capability, which is essential for studying rapid dynamics in biological organisms, such as blood flow, cardiac contractions, and motility of microorganisms in a three-dimensional volume.

关键词： Deformable mirrors adaptive optics nonlinear optical microscopy harmonic generation microscopy non-invasive in vivo imaging three-dimensional imaging multi-contrast microscopy

来源：评论

学校读者我要写书评

暂无评论

Dynamic structured illumination microscopy: focused imaging and optical sectioning for moving objects

Dynamic structured illumination microscopy: focused imaging ...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xiII

作者： Krzewina, Leo G. Kim, Myung K. Univ S Florida Dept Phys Tampa FL 33620 USA

ISBN: (纸本)0819461326

Structured illumination microscopy (SIM) is a valuable tool for three-dimensional microscopy and has numerous applications in bioscience. Its success has been limited to static objects, though, as three sequential image acquisitions are required per final processed, focused image. To overcome this problem we have developed a multicolored grid which when used in tandem with a color camera is capable of performing SIM with just a single exposure. images and movies demonstrating optical sectioning of three-dimensional objects are presented, and results of applying color SIM for wide-field focused imaging are compared to those of SIM. From computer modeling and analytical calculations a theoretical estimate of the maximum observable object velocity in both the lateral and axial directions is available, implying that the new method will be capable of imaging a variety of live objects. Sample images of the technique applied to lens paper and a pigeon feather are included to show both advantages and disadvantages of CSIM.

关键词： three-dimensional microscopy microscopy optical sectioning structured illumination

来源：评论

学校读者我要写书评

暂无评论

GPU-based image Registration in Aperture Correlation microscopy, and Reflection Mode Correlation microscopy

GPU-based Image Registration in Aperture Correlation Microsc...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Fafchamps, Lionel J. Neil, Mark A. A. Juskaitis, Rimas Imperial Coll London London England Aurox Ltd Abingdon Oxon England

ISBN: (纸本)9780819493583

Aperture Correlation microscopy (ACM) is a fluorescence microscopy technique capable of depth resolved imaging and enhanced lateral resolution at real-time acquisition rates. It relies on the subtraction of 2 separate images from different cameras which must be registered to the sub-pixel level. In order to achieve real-time registration and subtraction, the graphics processing unit (GPU) is used to apply a transformation from one frame to the other, resulting in a system capable of processing over 200 frames per second on modest hardware (GeForce 330M). Currently, this rate is limited by camera acquision to 16fps. Additionally, a novel reflection mode correlation microscope is introduced which functions on similar principles as the fluorescent system but can be used to examine reflective samples. images and z-stacks taken with this system are presented here.

关键词： Cameras microscopy image registration Microscopes Confocal microscopy image processing Objectives Polarizers image transmission Reflectivity

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：