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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI"

共 593 条记录，以下是71-80 订阅

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High-speed focal modulation microscopy using acousto-optical modulators for visualization of thick biological specimens

High-speed focal modulation microscopy using acousto-optical...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Chong, Shau Poh Gao, Guangjun Sheppard, Colin J. R. Chen, Nanguang Natl Univ Singapore Div Bioengn Singapore 117576 Singapore

ISBN: (纸本)9780819484413

In recent development of fluorescence microscopy, the out-of-focus fluorescence background that arises when imaging deep inside biological tissues is critical in determining the image quality and penetration depth. Focal modulation microscopy (FMM) is an advanced fluorescence technique that can provide high subcellular resolution when imaging thick specimens mainly by preserving the signal-to-background ratio.

关键词： Fluorescence microscopy multiple scattering penetration depth

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Scanning microscopy with extended depth of focus

Scanning microscopy with extended depth of focus

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xiI

作者： Juskaitis, R Botcherby, EJ Wilson, T Univ Oxford Dept Engn Sci Oxford England

We demonstrate a simple and light-efficient way of generating non-diffracting Bessel beams for use in confocal microscopy. A number of imaging modalities using such beams is discussed. Preliminary experimental results... 详细信息

ISBN: (纸本)0819456756

关键词： confocal extended depth of focus Bessel beam fluorescence microscopy polarisation multi-photon

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Detection, imaging and kinetics of sub micron organelles of chondrocytes by multiple beam interference microscopy

Detection, imaging and kinetics of sub micron organelles of ...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xi

作者： Joshi, NV Medina, H Barboza, JM Colantuoni, G Quintero, M Hosp Univ Los Andes Dept Physiol Fac Med Merida Venezuela

ISBN: (纸本)0819452327

Chondrocytes, obtained from testosterone treated human articular cartilage, were examined by a recently developed Multiple Beam Interference microscopy (MBIM) attached to a confocal set up, Video-enhanced differential interference microphotography and also by cinematography. In the MBIM, the intensity of the transmitted pattern is given by the Airy function which increases the contrast dramatically as the coefficient of the reflectance of the parallel plates increases. Moreover, in this configuration, the beam passes several times through a specific organelle and increases its optical path difference both because of the increase in the trajectory and refractive index (high density) of the organelle. The improved contrast enhances the resolving power of the system and makes visible several structural details of sub micron dimensions like nucleolus, retraction fibers, podia, etc. which are not possible to reveal with such a clarity by conventional techniques such as bright field, phase contrast or DIC. This technique permits to detect the oscillatory and rotational motions of unstained cilia for the first time. The frequency of oscillations was found to be 0.8 HZ. Keywords: Multiple Beam Interference Microscope, Chondrocytes Nucleolus and microfilaments, cilium and oscillatory & rotatory motion.

关键词： multiple beam interference microscope chondrocytes nucleolus and microfilaments cilium and oscillatory & rotatory motion

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Optical design of a snapshot high-sampling image Mapping Spectrometer (IMS) for hyperspectral microscopy

Optical design of a snapshot high-sampling Image Mapping Spe...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Gao, Liang S. Kester, Robert T. Tkaczyk, Tomasz S. Rice Univ Dept Bioengn Houston TX 77005 USA

ISBN: (纸本)9780819479662

A snapshot high-sampling image Mapping Spectrometer (IMS) is developed for hyperspectral microscopy, measuring datacube of dimensions 285x285x60 (x,y,lambda). microscopy IMS is designed to work at the Nyquist sampling limit to realize high resolution imaging. The spatial resolution is similar to 0.45 mu m with FOV similar to 130 mu m. The spectral working range is 500-700nm with similar to 8.3nm average spectral resolution. Preliminary tests have been implemented on its imaging performances.

关键词： Hyperspectral microscopy image Mapping Spectrometer

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three-dimensional state space realization algorithm: noise suppression of fluorescence microscopy images and point spread functions

Three-dimensional state space realization algorithm: noise s...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xiI

作者： Lai, XM Ward, ES Lin, ZP Ober, RJ Univ Texas SW Med Ctr Ctr Immunol Dallas TX 75235 USA

ISBN: (纸本)0819456756

A recently developed algorithm is applied to calculate a state space realization of a 3D microscopy image set. It is based on interpreting the image set as the impulse response of a 3D se arable system. As an application it is shown how this algorithm. combined with approximation steps, can be used to suppress noise in 3D experimental point spread functions. The approach was motivated by a well known problem that a noisy point spread function degrades the results of deconvolution algorithms for the restoration of 3D fluorescence microscopy image sets. The proposed approach can also be applied to 3D fluorescence microscopy image sets of cells.

关键词： point spread function estimation state space algorithm noise suppression

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Lateral resolution enhancement in confocal self-interference microscopy

Lateral resolution enhancement in confocal self-interference...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing xiI

作者： Kang, D Gweon, D Korea Adv Inst Sci & Technol Med Engn Dept NOM Lab Taejon 305701 South Korea

ISBN: (纸本)0819456756

Lateral resolution enhancement in confocal self-interference microscopy (CSIM) is evaluated. CSIM, which uses the birefringence of the calcite plate to generate self-interference pattern, sharpens the central lobe of the effective spot. Numerical simulation results of two-dimensional imaging performances are presented. Two-point resolution of 149nm is achieved, which is enhanced by nearly 100% compared to that of confocal microscopy.

关键词： confocal microscopy superresolution birefringence self-interference

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Fluorescence microscopy with isotropic resolution using three objectives 13

Fluorescence microscopy with isotropic resolution using thre...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XxiII

作者： Huelsnitz, Thomas Kner, Peter Univ Georgia Coll Engn Athens GA 30602 USA

ISBN: (纸本)9781628419474

Widefield and confocal fluorescence microscopy using a single objective suffer from poor resolution and a strong anisotropy between the lateral and axial resolution. Coherently combining the excitation and emission from two coaxial objectives improves the axial resolution up to sevenfold, but leaves the lateral resolution unchanged. Here we investigate the coherent combination of three objectives to create a point spread function (PSF) that is isotropic with higher resolution in the plane of the objectives. We develop a theoretical framework for simulating the performance of interferometric imaging with three objectives. Using three identical objectives with a large working distance and 0.9 numerical aperture (NA), the full-width half maximum of the confocal PSF is 135 nm compared to the lateral FWHM of 274 nm for imaging with a single objective at a wavelength of 515 nm.

关键词： microscopy fluorescence microscopy interferometric imaging PSF Engineering

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three-dimensional measurement of microorganism by retardation modulated differential interference contrast microscope

Three-dimensional measurement of microorganism by retardatio...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xiII

作者： Aoki, G. Itoh, M. Yasuno, Y. Yatagai, T. Univ Tsukuba Inst Appl Phys 1-1-1 Tennodai Tsukuba Ibaraki 3958573 Japan

ISBN: (纸本)0819461326

We propose a new technique for obtaining three-dimensional phase distribution on differential interference contrast microscope to modulate relative phase retardation between two shear beams. Using partial coherent theory we extract the phase information from two different retardation images. For the object in a weak phase region, simple formula is derived. The images of nematomorph were obtained in vivo.

关键词： differential interference contrast retardation modulated phase measurement microscope Nomarski

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Deformable mirror based remote focusing for fast three-dimensional microscopy 13

Deformable mirror based remote focusing for fast three-dimen...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XxiII

作者： Zurauskas, Mantas Frade, Maria Booth, Martin Univ Oxford Ctr Neural Circuits & Behav Mansfield Rd Oxford OX1 3SR England Univ Oxford Dept Engn Sci Parks Rd Oxford OX1 3PJ England

ISBN: (纸本)9781628419474

We present a deformable mirror based remote focusing method for three-dimensional imaging in high-resolution microscopy systems. The method relies on predefined mirror mode arrays that are obtained during initial mirror training step with a low complexity wavefront-sensing module. The imaging plane can be refocused over distances over a hundred times greater than the original depth of field of the objective lens along the optical axis at millisecond rates. We will demonstrate the combination of the remote focusing method with spatiotemporally focused two-photon excitation applied to three-dimensional imaging of biological samples.

关键词： Remote focusing adaptive optics three dimensional microscopy two photon microscopy

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Inverse scattering with subwavelength resolution in the near-zone

Inverse scattering with subwavelength resolution in the near...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Carney, PS Schotland, JC Washington Univ Dept Elect Engn St Louis MO 63130 USA

ISBN: (纸本)0819439398

In classical microscopy and inverse scattering, images are made from far field measurements. It will be shown that the standard Abbe or Rayleigh resolution limits arise as a result of assumptions which do not hold in the near field. Existing technologies such as TIRM and PSTM that exploit these properties of the near field produce two dimensional images which exhibit structure on a subwavelength scale. These images have proven difficult to interpret when the sample presents variations in structure and optical properties simultaneously in three dimensions. We present the analytic singular value decomposition of the linearized scattering operator. This representation leads to a robust inversion algorithm for the inverse scattering problem in the near zone. Numerical implementation of the algorithm is demonstrated using simulated noisy data to produce tomographic images of a three dimensional scatterer.

关键词： near-field optics inverse scattering microscopy tomography

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